This study was aimed at exploring the viability of osteoblasts on conductive tissue engineering material. Conductive biodegradable Polyprrole/Polylactide (PPy/PLA) was prepared by emulsion polymerization. Scanning electron microscope (SEM) and spectroscopy showed evenly dispersed PPy in PLA. PPy/PLA membrane was found being able to keep conductive stability for more than one month to provide electric circumstances (ECs) for osteoblasts. SEM displayed that osteoblasts adhered and spread well on PPy/PLA. ECs of 12.5, 25, 50, 75, 100, 125, 150, 175, 200 microA/cm2 were separately used to stimulate osteoblasts for 24h, 48h, 72h and 96h. Methyl-thiazole-tetrazolium (MTT) assay after 24h revealed that 50 microA/cm2 evidently accelerated osteoblasts proliferation. Alkaline phosphatase (ALP) activity assay revealed that, 48h later, 50 microA/cm2, and 75 microA/cm2 promoted osteoblasts differentiation. 50 microA/cm2 enhanced osteoblasts mineration. Conclusively, 50 microA/cm2 can strengthen osteoblasts' function and promote their viability.
Absorbable Implants ; Biocompatible Materials ; chemistry ; metabolism ; Cell Differentiation ; Cell Survival ; Cells, Cultured ; Electric Conductivity ; Electric Stimulation ; Humans ; Osteoblasts ; cytology ; Polyesters ; chemistry ; Polymers ; chemistry ; Pyrroles ; chemistry ; Tissue Scaffolds ; chemistry

In order to provide experimental data for the development and application of drug in clinic, we determined the effects of extracts from different parts of folium perillae (L. ) Britt. on hemorheological parameters, extracted from leaves (folium perillae), seeds (fructus perillae) and peduncles (caulis perillae). The results showed that all extracts from different parts of folium perillae (L. ) Britt. can significantly reduce the whole blood viscosity at low shear rate (10 s(-1)), erythrocyte aggregation index, erythrocyte electrophoresis index (P<0.05), and the whole blood reductive viscosity at low shear rate (10 s(-1)) (P<0.01). Extracts from folium perillae and caulis perillae can significantly decrease erythrocyte deformation index (P<0.05), whereas extracts from fructus perillae can not. Extracts from fructus perillae and caulis perillae can significantly decrease plasma viscosity at low shear rate(10 s(-1)), but extracts of folium perillae can not. Aspirin can only decrease the whole blood reductive viscosity at low shear rate and plasma viscosity (P<0.05). All extracts from different parts of folium perillae (L. ) Britt. had no significant effects on hematocrit, erythrocyte rigidity index, fibrinogen concentration , the whole blood viscosity and the whole blood reductive viscosity at middle and high shear rate (60 s(-1),120 s(-1)).
Animals ; Blood Viscosity ; drug effects ; Erythrocyte Aggregation ; drug effects ; Erythrocyte Deformability ; drug effects ; Male ; Perilla frutescens ; anatomy & histology ; chemistry ; Plant Extracts ; pharmacology ; Plant Leaves ; chemistry ; Plant Stems ; chemistry ; Random Allocation ; Rats ; Rats, Wistar ; Seeds ; chemistry

This study is to quantify time-varying dominant frequencies in electrocardiogram (ECG) during the ventricular fibrillation (VF). Orthogonal ECG (sagittal, x; transverse, y; and longitudinal, z) and the transvenous two-leads defibrillation systems were set in 19 dogs. Time-frequency analysis was used to assess changes in the dominant frequency within ECG recorded in dogs during trials of 10-30 s of VF. In 4 additional dogs, the dominant frequencies were compared during 10 s of VF before and after administration of amiodarone. Results showed that in the 427 trials of 10 s VF and 335 trials of 30 s VF, average variation in the dominant frequency was considerable, between +/- 12%-18%. The frequencies orthogonal ECG during 10 s VF were distributed symmetrically above and below the mean frequency like a normal distribution. In the 79 trials with administration of amiodarone during 10 s VF in all three ECG, the mean frequencies decreased from 6.5 (x), 7.4 (y) and 7.0 Hz (z) to 6.1, 6.4 and 6.3 Hz (P < 0.01), respectively, and the variation in dominant frequencies decreased from 1.18, 1.38 and 1.19 to 0.98, 1.11 and 1.02 Hz (P < 0.05) respectively. The results confirmed that the frequencies of 10-30 s VF in ECG vary considerably and continuously, and amiodarone decreases this variation.
Amiodarone ; pharmacology ; Animals ; Anti-Arrhythmia Agents ; pharmacology ; Dogs ; Electric Countershock ; Electrocardiography ; drug effects ; Female ; Male ; Ventricular Fibrillation ; physiopathology