Animals ; Humans ; Influenza A virus ; genetics ; immunology ; Influenza Vaccines ; administration & dosage ; genetics ; immunology ; Influenza, Human ; prevention & control ; virology ; Vaccines, Virus-Like Particle ; administration & dosage ; genetics ; immunology

OBJECTIVEDiffuse panbronchiolitis (DPB) is a chronic progressive disease of the lower respiratory tract, which is prevalent in Asian population. So far, many DPB cases have been found in adults in China. To our knowledge, no pediatric DPB case has ever been reported in China. We describe the first pediatric DPB case in Chinese literature and the second case in the English-language literature.

METHODThe clinical manifestations, characteristic imaging and histological features of this DPB case were summarized.

RESULTSThe patient was a 13-year old girl complained of chronic productive cough with wheezing. Chest auscultation revealed fine moist rales and wheezing over both lung fields. The chest X-ray showed small nodules and reticular opacities in left lower lobe. High-resolution thorax computerized tomography (HRCT) demonstrated bilateral diffuse small centrilobuler nodules and bronchial wall thickening or bronchiectasis in some parts of the lungs. Histopathological examination of transbronchial biopsy specimen revealed lymphocytes and foamy histocytes infiltrated in the walls of bronchi, respiratory bronchioles and adjacent alveoli. Lymphoid follicles were present around some bronchi. Sinus radiographs revealed sinusitis. Lung function studies showed obstruction and restriction. PaO2 was 65 mm Hg. The diagnosis of DPB was made according to the current diagnostic criteria. Low-dose erythromycin [5 - 10 mg/(kg.d)] was effective.

CONCLUSIONDPB could occur in children in China. The major diagnostic clues may include the following: (1) persistent cough, sputum, and dyspnea; (2) coexistent chronic sinusitis; (3) bilateral diffuse small nodular opacities on HRCT. Low-dose erythromycin was effective in treatment of the case with DPB.

Adult ; Bronchiolitis ; pathology ; China ; epidemiology ; Chronic Disease ; Cough ; blood ; diagnosis ; etiology ; Diagnosis, Differential ; Female ; Humans ; Lung ; pathology ; Pulmonary Disease, Chronic Obstructive ; diagnosis ; pathology ; Respiratory Function Tests ; methods ; Sputum ; microbiology

Objective To construct recombinant headless HA of avian influenza H5N1virus based on baculovirus expression system.Methods Headless HA gene of an avian H5N1virus,A/Hubei/1/2010 was cloned into pFastBac1vector.The recombinant shuttle vector,headless HA-Bac,was obtained by transforming pFastBac1headless HA into DH10bac cell and then the baculovirus bearing with headless HA gene,headlessHA-Bac,was achieved viaBac-to-Bac baculovirus expression system. The headless HA expression was detected in headless HA-Bac-infected Sf9 cells by Western Blot and HA assay.Results The headless HA has been efficiently expressed and displayed no reaction with turkey red blood cells.Conclusion The constructs provide fundamental work for future studies on universal influenza vaccine.

Objective To explore the effect of enteral nutrition with nursing intervention on the prognosis of the patients with severe intestinal dysfunction in Neurology.Methods One hundred and twenty patients were chosen and randomly divided into the control group (n =58) and the observation group (n =62)from July 2011 to June 2012.The control group received enteral nutrition and routine care.The observation group received enteral nutrition and complications of care on the basis of the routine care.The course of disease,the cure rate,mortality,sequelae and complications were observed in patients.The serum total protein (TP),serum albumin (ALB) and serum precursor protein (PA) were detected in the two groups.Results The course of disease was (55.2 ± 8.3) d in the observation group,shorter than (87.7 ± 10.5) d in the control group,the difference was statistically significant (t =-18.872,P ＜ 0.05).The cure rate was 95.2％ in the observation group,higher than 36.2％ in the control group,the difference was statistically significant (x2 =77.235,P ＜0.05).The mortality (one case,1.6％) and sequelae (six cases,8.6％) of the observation group were lower than those of the control group (five and thirteen cases,respectively),the difference between the control and observation group was statistically significant (x2 =5.062,5.985,respectively; P ＜ 0.05).The major complications of upper gastrointestinal bleeding,electrolyte imbalance,liver and kidney dysfunction and neurological impairment was respectively 0,5,2,10 cases in the observation group,and 9,16,10,23 cases in the control group,the difference was statistically significant (x2 =10.401,7.910,6.541,8.319,respectively;P ＜0.05).The TP,ALB,PA in the observation group were significantly increased after the treatment,and higher than those of the control group [(68.2 ± 2.3) / (61.5 ± 2.1) g/L,(36.8 ± 2.1) / (31.7 ± 2.0) g/L,(251.8 ± 30.01)/(173.1 ± 27.6)mg/L],the difference was statistically significant (t =14.149,12.842,10.792; P ＜ 0.05).Conclusions The enteral nutrition with nursing intervention can effectively shorten the patient's course of disease,improve the cure rate,reduce mortality and complications and improve the TP,ALB and PA in serum.

Nucleoprotein (NP) of influenza virus is highly conserved and type-specific. NP can trigger strong cell-mediated immune responses in host and is involved in the protection against the challenges with different subtype influenza viruses. Here, NP of an avian H5N1 (A/Hubei/1/2010, HB) was expressed by baculovirus surface-display technology and its immunogenicity as well as protective mechanism was investigated in mice infection model. Western blot and immunolabeled electron microscopy assay showed NP was displayed on baculovirus surface. ELISA results showed NP could induce high level of anti-NP IgG in the sera from NP-Bac-inoculated mice. Two cellular immune peptides (NP57-74 IQNSITIERMVLSAFDER and NP441-458 RTEIIKMMESARPEDLSF) were identified by IFN-gamma ELISPOT assay. NP57-66 and NP441-450 and NP protein could be able to trigger the activation of CD4+ and CD8+ T cells, and the response of CD8+ T was more predominant. The challenge study of mice-adapted virus A/PR/8/34 (H1N1) showed that NP-Bac could reduce viral load and attenuate the damage to lung tissue. 50% protection ratio against the virus could be detected.
Animals ; Antibodies, Viral ; immunology ; Baculoviridae ; genetics ; metabolism ; Cross Protection ; Enzyme-Linked Immunospot Assay ; Female ; Humans ; Immunity, Cellular ; Influenza A Virus, H1N1 Subtype ; genetics ; immunology ; Influenza A Virus, H5N1 Subtype ; genetics ; immunology ; Influenza, Human ; immunology ; virology ; Mice ; Mice, Inbred BALB C ; RNA-Binding Proteins ; genetics ; immunology ; T-Lymphocytes ; immunology ; Viral Core Proteins ; genetics ; immunology