The incidence of the lung cancer increased as the aging of population. Lung cancer in the aged characterized in feature and treatment. This paper discussed the characteristics, early features, the problems of routine treatment, the alternative managements such as green therapy, assessment and improvement of quality of life, etc., in old lung cancer.

Objective Observing the effect of general anesthesia (GA) with remifentanil on parturients undergoing cesarean section and Apgar score of child.Methods 40 parturients were divided into two groups,GA combined remifentanil (group A,n =20) and standard GA(group B,n =20).Observe the change of hemodynamics parameter and Apgar score of child.Results In group A than in group B maternal hemodynamic index smooth,two groups of intubation after lmin SBP,DBP,HR decreased,while the5 min after intubation returned to normal.SBP DBP,HR before induction,the difference between groups was not statistically significant (P＞ 0.05),the two group after induction,SBP,DBP,HR intubation after 1 min were (120.8 ± 19.6 mm Hg,80.6 ± 11.2 mm Hg,98.1 ± 17.1 BPM),(128.1 ± 17.5 mm Hg,98.9 ± 13.8mm Hg,108.7 ± 20.7 BPM) after intubation of 5 min were (98.3 ± 24.7 mm Hg 75.3 ± 13.8 mm Hg,79.9 ±22.6 BPM),(121.5 ± 16.2 mm Hg,88.6 ± 13.7 mm Hg,110.5 ± 17.4 BPM) had a significant difference (P ＜ 0.05),neonatal 1 min,5 min Apgar score,no significant differences between the two groups (P ＞0.05).Conclusion When remifentanil is used in the general anesthesia,its inducing is smoothly and it is no significant bad effect on the newborn.

Objective To investigate the regulatory effect of transforming growth factor-? 1 (TGF-? 1)on the proliferation and apoptosis of human prostatic epithelial cells in vitro. Methods The primary culture was prepared by collagenase dissociation of minced prostatic tissue obtained from 8 BPH patients (53 to 70 of age) on open prostatectomy and from 5 organ donors (23 to 36).Cells were cultured in serum free prostate epithelial cell growth media and identified by immunocytochemical studies.The cells were passaged and cultured in media free of bovine pituitary extract and EGF and TGF-? 1 and EGF in different doses were then added either alone or in combination according to experimental requirement.The rate of cell proliferation was assessed by counting the cell number and by 5-bromo-2'-deoxyuridine (ELISA).Apoptosis induced by TGF-? 1 was examined by in situ transferase-mediated dUTP nick and labeling. Results The cultured cells had the typical morphological features of epithelium and stained positive for PSA and Pan-Keratin.The cells from normal prostates of young donors showed more mitogenic activity than those from BPH prostates.EGF enhanced cell proliferation whereas TGF-? 1 inhibited cell proliferation which could be reversed by increasing concentration of EGF.TGF-? 1 induced apoptosis of human prostatic epithelial cells.The effect of TGF-? 1 and EGF was concentration dependent. Conclusions TGF-? 1 is a direct physiological regulator of cell proliferation and cell apoptosis of human prostatic epithelial cells.

Objective To investigate the effect of TGF ? 1 and EGF on the proliferation of cultured human prostatic epithelial cells. Methods The primary culture was prepared by collagenase digestion of minced prostatic tissue obtained from prostatectomies of 8 benign prostatic hyperplasia(BPH) patients (aged 53 to 70) and 5 organ donors (aged 23 to 36). Cells were cultured in serum free prostatic epithelial cell growth media and identified by immunocytochemical method. The cells were passaged and cultured in media without bovine pituitary extract (BPE) and then, EGF and TGF ? 1 with different concentration were added into the culture either alone or in combination according to the experimental protocol. The cell proliferation was assessed by counting the cell number (BrdU) and ELISA. Results Cultured cells showed typical morphological features of epithelium and stained positive for PSA and Pan Keratin. After 5 7 passages cells went senescence. BPE and EGF enhanced whereas TGF ? 1 inhibited the cell proliferation and the inhibition was reversed by increasing the concentrations of EGF. The effect of both TGF ? 1 and EGF displayed concentration dependent manner. Conclusions Cell proliferation of the cultured human prostatic epithelial cells could be modulated by TGF ? 1 and EGF. These results indicated that TGF ? 1 and EGF, as the negative and positive growth factors might play an important role in the pathogenesis of BPH.

Objective To study the feasibility of laparoscopic procedures in the treatment of ectopic pregnancy with hemorrhagic shock. Methods Laparoscopic procedures were carried out in 35 patients with ectopic pregnancy concurrent with hemorrhagic shock. Patients' blood pressures were all above 60/30 mmHg, and B-ultrasonography revealed the intra-abdominal blood loss

To improve the precision of image registration based on corner detection, a relative position function between multiple points to determine matching points accurately. First the corners in images are detected using Harris detector, and clustering method is used to eliminate most wrong matches after coarse screening. Then the proposed relative position function is used as a criterion of precise matching. Finally the image registration process is accomplished by affine transformation. Results show that the proposed algorithm is more effective and accurate than conventional registration algorithm.

Objective: To study the diagnosis and treatment of primary hyperparathyroidism.Methods: The clinical data of 8 primary hyperparathyroidism patients(1992 2002) in our hospital were summarized and the references were reviewed.Results: It was found that primary hyperparathyroidism was liable to be misdiagnosed.Information on blood calcium,parathyroid hormone(PTH) and imaging may be helpful for diagnosis. Surgical resection(4 cases), anhydrous alcohol injection (2 cases), electron radiation (1 case) and internal medicine (1 case) were applied. Conclusion:Information on blood calcium,PTH and early imaging is helpful for diagnosis.Operation is the first choice of treatment, and for patients who can not tolerate surgery, anhydrous alcohol injection is an effective and safe treatment.

ObjectiveTo assess rat behavior, observe apoptotic morphology of neurons and measure expression of caspase-3 in substantia nigra(SN) of Parkinson's disease(PD) animal model. Methods6-OHDA was stereotacticly injected into the right striatum of the rats at two sites to produce PD models. After 5 weeks, the behavior tests including modified Morris Water Maze and narrow beam test were measured. Then tyrosine hydroxylase (TH) histochemistry, Hoechst 33258 staining, Western blotting for caspase-3 in right substantia nigra was separately conducted.ResultsEscape velocity significantly decreased and its latency was obviously enlarged in modified Morris Water Maze, and the latency and total time in narrow beam test were also markedly increased (P<0-05) in 15 successful PD rats compared with either the sham group or the normal group. Furthermore, there were obvious less TH-positive neurons in lesioned SN while more apoptotic cells appeared there. In addition, the expression of caspase-3 was highly upregulated in lesioned SN.Conclusion6-OHDA induced neuronal apoptosis in SN is associated with high levels of caspase-3, and the results of rat behavior tests are correspondent with the morphological changes including TH immunohistochemistry and Hoechst 33258 staining. Thus modified Morris Water Maze and narrow beam test are beneficial for assessments of effects of new drugs in PD animal model.

ObjectiveTo investigate the protective effects of epigallocatechin gallate (EGCG) in a mouse model of Parkinson's disease induced by 1-Methyl-4-phenyl-1,2,3,6 tetrahydropyridine (MPTP).Methods32 C57BL/ 6 male mice were randomly divided into 4 groups: Model group was administrated with 16 mg/kg MPTP (i.p., four times, 2 h interval); Sham group was treated with saline; EGCG treatment group was given EGCG (5 mg/kg) after MPTP administration; normal group was just given EGCG (5 mg/kg) as treatment group. After given EGCG for 3 weeks, behavioral tests, tyrosine hydroxylase (TH) immunohistochemistry staining and the HPLC for dopamine (DA) and its metabolites were used.ResultsThe present results indicated that oral administration of EGCG significantly improved the behavioral impairement in mice induced by MPTP (P<0.05). And in the EGCG treatment group, there were more TH-positive neurons than in model group. In addition, levels of DA and its metabolites in striatum decreased significantly in MPTP group (P<0.05). Though the concentration of DA and its metabolites in EGCG treatment group tended to increase, however, there was no significance between EGCG treatment and model group.ConclusionEGCG could improve the behavioral impairment in a mouse model of Parkinson's disease induced by MPTP and protect against the loss of the dopamine neurons in the substantia nigra (SN).

Objective To study the oxidative stress and apoptosis relative protein expression in rat striatum during the pathogenesis of Parkinson's disease (PD) induced by 6-hydroxydopamine (6-OHDA). Methods 6-OHDA was stereotacticly injected into the right striatum of the rats at two sites to produce PD models. Activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), contents of reduced glutathione (GSH) and malondialdehyde (MDA) in the injured and normal striatum were measured using assay kits; and levels of Bax and Bcl-2 were detected by Western blotting in injured striatum. Results In 10 successful PD rats, compared with either the sham group or the normal group, activities of SOD and GSH-Px and contents of GSH in the right striatum significantly decreased while contents of MDA increased obviously (P<0.05); And levels of Bax significantly increased while expression of Bcl-2 obviously decreased. Conclusion Oxidative stress plays a key role in the pathogenesis of PD. Furthermore, Bax and Bcl-2 were involved in the regulation of apoptosis under oxidative stress induced by 6-OHDA.