Objective To investigate and analyse the status and influential factors of nurses' palliative care knowledge in Shanghai city.Methods Using cluster sampling method,a questionnaire survey using Palliative Care Quiz for Nursing (PCQN) was conducted on 973 nurses in 9 hospitals of Shanghai city.Results 939 nurses completed the survey whose scores were 8.88±2.51.The status quo of palliative care knowledge of Shanghai nurses were not very desirable.The scores of palliative care philosophy and principles and psychological,social and spiritual support were 0.376±0.009 and 0.310±0.008.The scores of knowledge of the pain and symptomcontrol was 0.496±0.005,which was better than the other two aspects.There were differences between dimension 1 and dimension 2,3 (t=-1 1.78,5.465,P＜0.01),and also between dimension 2 and 3 (t=19.673,P＜0.01).Conclusions Lacking of education and training,insufficient recognition of related institutions and the limit of traditional ideas caused the defect in the knowledge structure of palliative nursing.

Objective To investigate the effect of early rehabilitation treatment in the patients with small cerebral hemorrhage and its possible mechanisms .Methods 133 patients with cerebral hemorrhage were randomly divided into the routine treatment , non-early rehabilitation and early rehabilitation groups and given the routine drug treatment .The early rehabilitation group and the non-early rehabilitation group were additionally given the rehabilitative training after 2 d and 14 d respectively .The scores of Fugl-Meyer reassessment and the Barthel index were assessed before training and after 4-week training ,and the serum levels of circulat-ing endotheial progenitor cells(EPCs) and vascular endotheial cell growth factors(VEGF) were detected .Results Compared with the routine treatment group and the non-early rehabilitation group ,the scores of Fugl-Meyer reassessment and the Barthel index af-ter treatment in the early rehabilitation group were significantly increased (P< 0 .01) .The serum levels of circulating EPCs and VEGF in the early rehabilitation group were also significantly increased compared with the routine treatment group control (P<0 .01) .Conclusion Early rehabilitation treatment can obviously improve the limb movement function and the daily living ability in the patients with small cerebral hemorrhage ,its mechanisms may be involved with the increase of circulating EPCs and VEGF .

Objective: To examine and analyze the expression of two isoforms of livin (livin α/livin β), a novel inhibitor of apoptosis protein (IAP) family member, in tumor cell lines and malignant tumor tissues and analyze the relationship between livin expression and clinical pathological features of different tumors. Methods: Livin mBNA expression was detected by reverse transcription polymerase chain reaction (BT-PCR) in 12 malignant tumor cell lines, 50 different malignant tumor tissues, and 20 cases of normal tissues. Results: Livin mBNA was highly expressed in seven cell lines (Hela, SPEA-1, SBE-2, PC14, MKN45, LOVO, and HHCC) and poor expressed in A549 cells, It had negative expression in other four kinds of cell lines ( HL-7702, Hep-2, 7721 and K562). Livin mRNA was positively expressed in 32 of 50 malignant tumor cases (64.0%). The expression of livin mRNA was at low level in paracancerous tumors tissues (2%). Livin expression had no significant association with differentiation degree, lymph node metastasis and TNM staging of tumor patients. Conclusion: Livin expression in tumor cell lines and malignant tumor tissues provides stronger evidence for further investigating the relationship between livin and tumor initiation and development. It may serve as a new target for malignant tumor diagnosis and therapy.

OBJECTIVE To investigate the drug-resistance status of clinically isolated Staphylococcus aureus(SAU) and provide scientific evidence for clinically reasonable use of antibiotics.METHODS Retrospective review was adopted to analyze the specimen source and the clinical distribution of 311 SAU strains.BioMerieux VITEK 32 was used to identify SAU.Antibiotic susceptibility testing was performed by K-B method.RESULTS Isolating rate of meticillin-resistant S.aureus(MRSA) was 48.9%.SAU were resistant to the diverse antibiotics in different degree except for 100% sensitivity to glycopeptide antibiotics.The drug-resistance to antibiotcs in MRSA were all higher than those in meticillin-sensitive S.aureus(MSSA).CONCLUSIONS Monitoring and controlling of antibiotics resistance are effective measures of prevention from nosocomial infections.

[Objective]To compare the clinical results between allograft and autograft for treatment of scoliosis in the adolescent.[Method]A retrospective study was made in 63 cases of adolescent patients with scoliosis who were operated on with posterior instrumentation from 1996~2006.These patients were divided into two groups.In group A,32 patients aged 10 to 15 years with an average of 12.2 years,had a mean Cobbs angle of 62?(ranged,38? to 113?),were received allograft bone grafting.The patients were followed up for at least 18 months with an average of 26 months.Clinical and radiographic evaluations were performed.[Results]Operating time and blood loss in group A were less than that in group B(P

Objective To investigate molecular changes in growing rats masticatory muscle after functional mandibular protrusion.Methods SD rats were randomly divided into eight groups as control 3d,7d,14d,21d and experimental 3d,7d,14d,21d.The RTPCR and qPCR methods were used to find expression and any differences of mRNA of MMPs in growing rats masticatory muscle after functional mandibular protrusion. (Results)(① Expression) of MMP-2,MMP-9 mRNA expressed in all growing rats masticatory muscle.② Expression differences of MMP-2 and MMP-9 mRNA in 3d,21d were found between control and experimental groups after functional mandibular protrusion.Conclusion ①MMP-2 and MMP-9 mRNA participate in masticatory muscle normal development.(② MMPs) mRNA involve changes in growing rats masticatory muscle after functional mandibular protrusion.

AIM: To construct the eukaryotic expression vector CD80-IgG by fusing the cDNA encoding extracellular portion of murine CD80 to the 5'-terminus of cDNA encoding Fc fragment of murine immunoglobulin G1 and to express the fusion protein in Chinese hamster ovary (CHO) cells. METHODS: The two cDNAs was amplified by PCR respectively from plasmid pcDNA/B7 containing the full-length cDNA of murine CD80 from murine spleen cells, and cloned to the eukaryotic expression vector pcDNA3.0 by directional cloning. The resultant recombinant plasmid pcDNA/CD80-IgG was transfected into CHO cells with liposome transfection reagent. The stably expressing cells were obtained by G418 screening. Western blot, Dot ELISA, and flow cytometry were used to detect the expression of the fusion protein and its immunological activity. RESULTS: DNA sequencing verified the correction of the construction of recombinant plasmid pcDNA/CD80-IgG. The expressed fusion protein was detected in the supernatant of transfected CHO cells and the molecular weight of the protein was similar to what we expected. Its immunological activity was also established. CONCLUSION: The recombinant plasmid pcDNA/CD80-IgG was successfully constructed and it expressed the fusion protein CD80-IgG.

Cells, Cultured ; Humans ; Neovascularization, Physiologic ; immunology ; Pericytes ; metabolism ; physiology

Objective To analyze the influencing factors of perioperative coagulation dysfunction in neonates with gastrointestinal surgery.Methods Coagulation indexs, including plasma prothrombin time (PT), partial activated thromboplastin time (APTT), thrombin time (TT) and plasma fibrinogen (Fib), were collected retrospectively from June 2002 to April 2016 in gastrointestinal neonatal surgery, as well as perioperative clinical data and the perioperative coagulation dysfunction associated risk factors were analyzed.Results There were 158 cases with abnormal coagulation indexs within 4 hours after operation, including 61 cases of mild abnormalities and 97 cases of obvious abnormalities.Multivariate logistic regression analysis showed that preoperative pneumonia (OR=2.880, 95%CI 1.417-5.852), perioperative low calcium (OR=2.381, 95%CI 1.167-4.857) and invasive puncture catheter (OR=2.490, 95%CI 1.299-4.773) were independent risk factors for neonatal coagulation index abnormality within 4 hours after surgery (P<0.05).Conclusion In the perioperative management, pneumonia should be actively treated, correcting hypocalcemia and choosing concentrations of less than 6.25 U/ml of heparin solution or normal saline careing arteriovenous catheter reduces the occurrence of neonatal postoperative coagulation dysfunction.