Acute leukemia is one of the most common cancers among children.The biological mechanisms leading to leukemia have not been fully clarified until now.Experiments were improved that most patients developing acute leukemia had abnormal chromosomes,including TEL/AML-l,BCR/ABL,PML/RAR-α fusion genes,which would be potential clinical biomarkers.Many genetic polymorphisms have effect on acute lymphoblastic leukemia susceptibility,which were containing genes involved in folate metabolism pathways,cytochrome P450,glutathione-S-transferase enzymes and quinone oxidoreductase-1.As the results of the studies,alcohol,tobacco,TNF-α and IFN-γ might be risk factors to the disease.Furthermore infection and IRF-3 were reported to reduce the occurrence of acute leukemia.Finally,as more is learned about the molecular pathology,it may be possible to develop new therapeutic agents which are specifically targeted to treat childhood acute leukemia.

The medical image registration between preoperative three-dimensional (3D) scan data and intraoperative two-dimensional (2D) image is a key technology in the surgical navigation. Most previous methods need to generate 2D digitally reconstructed radiographs (DRR) images from the 3D scan volume data, then use conventional image similarity function for comparison. This procedure includes a large amount of calculation and is difficult to archive real-time processing. In this paper, with using geometric feature and image density mixed characteristics, we proposed a new similarity measure function for fast 2D-3D registration of preoperative CT and intraoperative X-ray images. This algorithm is easy to implement, and the calculation process is very short, while the resulting registration accuracy can meet the clinical use. In addition, the entire calculation process is very suitable for highly parallel numerical calculation by using the algorithm based on CUDA hardware acceleration to satisfy the requirement of real-time application in surgery.
Algorithms ; Humans ; Imaging, Three-Dimensional ; Tomography, X-Ray Computed ; X-Rays

STAT3 can be activated by various cytokines and participate in the process of tumor cells proliferation,angiogenesis,invasion and chemotherapy resistance.Epithelial-mesenchymal transition (EMT)also plays an important role in the process of tumor invasion and metastasis and chemotherapy resistance.Studies have shown that STAT3 can regulate the process of EMT so as to change the invasion and metastasis abilities of tumor cells,and to enhance their ability of chemotherapy resistance.Discussion of the role and relation of STAT3 and EMT in tumor cells will be helpful to provide reliable theory for tumor molecular targeted therapy.

Objective　To study the prognostic factors in cholangocarcinoma(CC). Methods　Retrospective analysis of the reported clinical data of CC in lectures. Results and Conclusions　The prognosis of CC in lower portion of bile duct(BD) is much better, CC in meddle portion of BD take the second place of prognosis, CC in upper portion of BD has the worst prognosis. If CC with following cases, which would have much better prognosis: higher differentiation without metastasis, diploid, higher PCNA; if CC with negative expression of epithelial membrane antigen or keratine, which would have worse prognosis. The activate of type IV collegen is related linearly to the metastasis capacity of the tumor, and the expression of nm23 of tumor is related to invation, lymphatic metastasis and prognosis of the tumor. Radical resection of the tumor has the best effect, palliative resection take the second place of prognosis, internal or external drainage operation has the worst effect. The effect of local radiotherapy is limited, and chemotherapy showes no effect. Early diagnosis, radical or extending resection and combined therapy can improve the prognosis of CC.

Objective To make a research on the expression of perlecan in the formation of epidural scar after posterior lumbar laminectomy in the rat.Methods Sixty Wistar rats were randomly and evenly divided into five groups:the experimental groups including A(the first week after surgery),B(the second week),C(the fourth week),D(the sixth week)and the control group.Laminectomy was performed on lumbar vertebra in each experimental group.The hematoxylin-eosin(HE)staining was used to observe the formation of epidural scar.Immunohistochemistry was performed to detect the perlecan located in the epidural scar.Western blot and RT-PCR were made to quantify the expression of the perlecan after surgery.Results According to HE,with prolonged,the scar tissue gradually formed.Immunohistochemistry demonstrated that the perlecan was expressed in the fibroblast.Western blot and RT-PCR showed the high level expression of perlecan in the experimental groups compared to the control group(P＜0.01).Furthermore,the expression of perlecan gradually increased with the prolongation of exposure time and had significantly statistical significance (P＜0.01)between the first three experimental groups.However,compared to the fourth week group,the expression of perlecan in the sixth week decreased obviously(P＜0.01).Conclusion Perlecan was expressed in the fibroblast of the epidural scar,and which plays an important role in the formation of epidural scar.

TCM boosts plenty theories and practice on delaying aging.Many TCM recognitions on the mechanism of female aging are still useful.The female has menstration,pregnancy,and lactation physiologically,which are different from the male,so the mechanism of the female aging is obviously different from the male.Many factors leads to female aging,such as the growth of age,interaction between deficiency,qi stagnation,blood stasis,and turbid phlegm,and results in functional retrogradation of the whole body.A deep discussion on the mechanism and law of female aging will bring about positive significance in the prevention and treatment of this disease.

Objective To study the variable number tandem repeats (VNTR) polymorphism in intron 4 of the endothelial nitric-oxide synthase (eNOS) gene and its relationship with plasma NO in pregnancy induced hypertension (PIH). Method The VNTR polymorphism in intron 4 of the eNOS genes of PIH and healthy control pregnant women were detected by PCR. The plasma level of NO~(3-)/NO~(2-) was measured by spectrophotometer, which was used as the index of NO synthesis.Data were analyzed according to the different type of genotypes and allelic gene frequency. Results The PIH group showed significant higher frequency of VNTR 4a allele and 4a/4b+4a/4a genotype than those of the control (16.00% vs 7.50%,P

OBJECTIVE:To probe into the optimum condition and dosage of activated carbon in the preparation of arilin injection.METHODS:The impacts of activated carbon of different dosage at different temperature,different storage time and different pH value of preparation on the contents of arilin injection were investigated and analyzed statistically.RESULTS:Positive correlation was found between the descending degree of the contents of arilin and the dosage of the activated carbon(P

OBJECTIVE:To study the effect of kallidinogenase on senile diabetic nephropathy.METHODS:95senile cases with diabetic nephropathy treated with the basic therapy were randomly divided into treatment group(plus kallidino?genase)and control group(plus aspirin),the proteinuria ejection level,hemorheological parameters and blood lipid level of2groups in24hours were investigated before and after treatment.RESULTS:As compared with prior treatment and the coutrol group,the proteinuria ejection level decreased significantly,the hemorheology improved and the blood lipid level decreased in the treatment group.CONCLUSION:Kallidinogenase is effective to some degree in the prevention and cure of senile diabetic nephropathy and diabetic macroangiopathy.