Objective To investigate the effects of platelet-derived growth factor(POGF),basic fibroblast growth factor(bFGF)and epidermal growth factor(EGF)on the proliferation,migration and phenotypie modulation of airway smooth muscle cells(ASMCs)in vitro.Methods Cultured human ASMCs were stimulated with PDGF.bFGF,EGF or DMEM(control).Cell pmliferation was detected by BrdU incorporation and cell count.Cell migration was measured using Boyden's chamber.Reverse transcription-poly-merase chain reaction(RT-PCR)and Western blot analysis were used for the detection of sm-α-actin and sm-MHC mRNA and protein expression in ASMCs.Resuits The mitotic index(%BrdU-positive per total number of cells)of ASMCs stimulated with PDGF.bFGF and EGF were significantly higher than those in control(P<0.001 or P<0.05).And the cell number of ASMCs stimulated with PDGF,bFGF and EGFwere also significantly higher than those in control(P<0.05).The number of migrating cells treated with PDGF,bFGF and EGF were higher than that in eontrol(P<0.05).The expressions of sm-α-actin mRNA treated with bFGF and EGF were significantly lower than those in control.but it was higher than control when treated with PDGF.The expressions of sm-MHC mRNA treated with bFGF and EGF were significantly lower than those in control,but it was higher than control when treated with PDGF(P<0.01 orP<0.05).Theexpressions of sm-α-actin protein treated with bFGF and EGF were significantly lower than those in control,but it was higher than control when treated with PDGF(P<0.05).The expressions of sm-MHC protein treated with bFGF and EGF were significantly lower than those in control.but it was higher than control when treated with PDGF(P<0.05).Conclusion PDGF,bFGF and EGF can directly induce ASMCs,proliferation and migration in vitro;and meanwhile,the expressions of cells,contractive phenotype increased treated with PDGF and decreased treated with bFGF and EGF.

Role of chronic inflammation in type 2 diabetes and obesity has been recognized in the past few years. Examples of potent inflammatory cytokines are C-reactive protein (CRP),tumor necrosis factor-?(TNF-?) ,plasminogen activator inhibitor-1 (PAI-1) and interleukin-6 (IL-6). Inflammatory processes may have important roles in the development of type 2 diabetes,obesity and insulin resistance. This article reviewed the relationship between some inflammatory cytokines and diabetes, providing references for clinical diagnosis and treatment.

Objective To investigate the use of antimicrobial drugs in our hospital and provide guide for clinical use of antimicrobial drugs.Methods Tabulate and analyze the usage of antimicrobial drugs according to the discharged patients' medical records from Jan 2004 to Dec 2006.Then use statistical methods for retrospective survey.Results Among 4003 medical records,2756 of them used antibiotics,with the percentage being 68.85%.1103 recodes used combined medication,which accounted for 40.02% of the total records.Type I incision occupied 90.86% in surgical departments.The percentage of the inpatients who used antimicrobial drugs after operation for about five to seven days was 78.85%.For selecting drugs,the majority were empirical antibiotics treatment,which achieved 93.72%.The ratio of irrational use of drugs was 25.90%.Conclusion The clinical use of antimicrobial drugs in our hospital is dramatically lower than the average level of the whole nation.However,non-standardized administrations of medication,super-standardization,super-range use of drugs are still the problems needed to solve.

This paper discussed the different ways on the pharmacology of traditional Chinese medicine teaching to cultivate students' autonomous learning ability, promoting independent learning through PBL teaching, enhancing students' ability to think independently and practical operation using heuristic teaching.Survey results showed this student-centered, teacher-led, student-teacher interaction style pharmacology teaching model could improve the quality of teaching of Pharmacology.

Objective To investigate the role of rosiglitazone in high glucose(HG)-induced intercellular adhesion molecule-1(ICAM-1) expression of rat mesangial cells.Methods The rat mesangial cells(MCs) were cultured in the medium with normal glucose(5.6 mmol/L,NG),high glucose(25 mmol/L,HG),HG+5 ?mol/L rosiglitazone,HG+20 ?mol/L rosiglitazone and HG+PDTC(?NF-?B inhibitor).ICAM-1 mRNA expression was measured by semi-quantitative RT-PCR assay.Activation of nuclear factor-?B(NF-?B) of rat mesangial cells was measured by electrophoretic mobility shift assay(EMSA).The levels of ICAM-1 in the supernatants were determined by enzyme-linked immunosorbant assay(ELISA.) Results RT-PCR results showed that high-glucose increased the ratio of PCR products of ICAM-1 over GAPDH to 2.9-fold,which was prevented by rosiglitazone(5 and 20 ?mol/L) pre-treatment.The NF-?B binding activity was 2.5-fold higher in MCs exposed to HG as compared with NG(P

BACKGROUND:The human body pulse signal can be regarded as the convolution of the heart excitation resource signal and the pulse transfer system. The backward signal was studied more before, but the research to the forward signal is not enough.OBJECTIVE: To extract the heart excitation source signals in human pulse wave.DESIGN: A randomized controlled design.SETTING: Second Affiliated Hospital of China Medical University;Biomedical Engineering institute of Shandong University.PARTICIPANTS: Health physical-examined persons in the Second Affiliated Hospital of China Medical University on March 11,2004 were recurited. RM6240 physiological signals collection system was adopted.METHODS: The collection of pulse wave in healthy persons was input into system-analysis mode and based on the principle of blind deconvolution and feasible arithmetic, heart source signals were obtained.MAIN OUTCOME MEASURES: ①Normal human pulse wave ②The heart excitation source signals.RESULTS: In the back-half segment of cepstrum (n＞ n0), periodic impulse was in accordance with the basic human pulse frequency. There was an excitation source in the pulse wave. If high-pass filter was defined to the signal (when n0 equals to 30 in the experimentation the result will be best.), we could get the heart excitation source signal after it was filtered, F transformation, index and inverse transformation.CONCLUSION: This method is practical and feasible and will provide a basis to further analyze pulse wave.

Cells, Cultured ; Humans ; Neovascularization, Physiologic ; immunology ; Pericytes ; metabolism ; physiology

Objective To observe the therapeutic effect and effects on coagulation indexes ofXuefu Zhuyu Capsules combined with chemotherapy in treating patients with mid-advanced small cell lung cancer (SCLC).Methods Totally 100 patients with mid-advanced SCLC were randomly divided into observation group and control group with 50 cases in each group. The observation group was given EP chemotherapy combined withXuefu Zhuyu Capsules, while the control group was given EP chemotherapy for 4 cycles. The coagulation indexes of two groups before and after treatment were observed, and the clinical efficacy and TCM syndrome efficacy were evaluated.Results After treatment, the tumor volume and TCM syndrome scores of two groups were lower than those before treatment (P<0.01), and the observation group was lower than the control group (P<0.01). The effective rate of the observation group was 100%, higher than 88% of the control group, the difference was statistically significant (P<0.05). The improvement rate of TCM syndrome score was 98% in the observation group, and was 84.0% in the control group. The observation group was superior to the control group (P<0.05). In observation group, D-dimer, platelet count and fibrinogen after treatment were lower than those before treatment (P<0.05,P<0.01), and were lower than those in control group (P<0.01). There was no significant difference of all kinds of adverse reactions between the two groups (P>0.05).Conclusion EP chemotherapy combined with Xuefu Zhuyu Capsules can improve hypercoagulable state in patients with SCLC, improve the effect of chemotherapy, and do not increase the adverse reactions.

Objective To discuss the reasons for the difference in the results of medical checkups for cardidates enlisted in teenager aviation classes, in order to offer tips for the establishment of regional training programs and improve the qualification rate and acceptability.Methods A total of 2894 junior high school graduates as candidates for selection to teenager aviation classes in 2015 were given a double-blind medical examination.Cross-sectional survey methodology was used to compare the differences in the results of physical examinations, for candidates from seven areas.Results The qualification rate of physical checkups in the above regions were significantly different.The regional differences in the rejection rate related to ophthalmology, surgery, neurology and ultrasound were significant(P <0.05),but the regional differences in the rejection rate in internal medicine and ENT were not(P>0.05).The vision, height and relative body mass in different regions were significantly different (P<0.05)except for blood pressure test (P>0.05).Conclusion The physical differences are the results of various factors.In order to improve the passing rate of candidats, the aviation training plan for teenager aviation classes should consider the geography, climate, socio-economic environment and the physical health of local students.We should implement training programs in line with local conditions reather than use a unified national standard.

Objective To investigate the characteristics in vitro and hemostatic function of O -carboxymethyl chitosan multi-hemostatic sponge. Methods Freeze-drying technology was applied on preparation of O -carboxymethyl chitosan hemostatic sponge. The physicochemical properties of the self-produced multi-hemostatic sponge were observed for its appearance, porosity rate,water absorption,and density. The hemostatic effect was compared between the self-produced hemostatic sponge and commercially available absorbable gelatin sponge in rabbit models with ear venous and arterial injuries. Results The self-produced hemostatic sponge was off-white and full of pores with good tenacity and even net texture. Its porosity ratio, water absorption rate, and density were 67. 23%,38. 77%,and 0. 043 4 g·(cm3)-1,respectively. The bleeding time volume were significantly lower from the self-produced sponge than that from the commercially available gelatin sponge. No secondary re-bleeding was observed. Conclusion The self-produced O -carboxymethyl chitosan multi-hemostatic sponge displays stable physicochemical characteristics and reliable hemostatic effect.