ObjectiveTo detect the expression on p53 gene in retinal ganglion cells(RGCs) of rabbit eyes with different degrees of intraocular hypertension.MethodsIntraocular pressure(IOP)was elevated by intracameral injection of 0.10～0.15 ml of 2% methylcellulose.Animals were grouped up by different values of IOP.The rabbit eyes were enucleated,fixed,paraffin-embedded and cut into serial 6- μm thin sections.For histological evaluation,the slides were stained with DAB chromogen(conducting an immuohistochemical experiment),and were examined by light microscopic and automatic image analysis.ResultsThe number of RGCs with a positive reaction for p53 monoclonal antibody was statistically higher in the experimental eyes than in their control fellow eyes(P<0.01),and apoptosis was mainly found in the early stage of research.ConclusionOur research presents a new alternative view of apoptosis in experimental glaucoma(According to former reports,apoptosis was mainly found in the advanced stage of the patients with POAG)

Gastric cancer is one of the most commonly seen malignant tumors,and is the fourth leading cause of morbidity and second leading cause of mortality among malignancies worldwide. The genesis of gastric cancer is the result of interaction between genetic and environmental factors,and is a multi-factor and multi-step carcinogenesis. As one of the most important members in the heat shock protein(HSP)family,HSP70 plays a molecular chaperone role,and is involved in body specific immunity and innate immunity. Studies have demonstrated that over expression of HSP70 often correlates with the genesis and development of gastric cancer. This article reviewed the advances in study on relationship between HSP70 and gastric cancer.

Objective To develop a new software system that cau directly display the predicted results on an electronic map,in order to get a directly perceived understanding of the affected areas of nuclear and radiological terrorism events in city areas.Methods Three scenarios of events including spreading radioactive materials, dirty bomb attack,and explosion or arson attacks on the radiation facilities were assumed.Gaussian diffusion model was employed to predict the spread and deposition of radioactive pollutants,and both the internal and external doses were estimated for the representative person by using the corresponding dose conversion factors.Through integration of the computing system and Mapinfo geographic information system(GIS),the predicted results were visually displayed on the electronic maps of a city.Results The new software system could visually display the predicted results on the electronic map of a city, and the predicted results were consistent with those calculated by the similar software Hotspot(R)The deviation between this system and Hotspot was less than 0.2 km for predicted isoplethic curves of dose rate downwind.Conclusions The newly developed software system is of the practical value in predicting the effects of nuclear and radiological terrorism events in city areas.

Objective To determ ine the norm al reference values of 33 elem ents, Ag, Al, As, Au, B , B a, Be, Ca, Cd, Co, Cr, Cs, Cu, Fe, G a, H g, Li, Mg, Mn, Mo, N i, Pb, Rb, Sb, Se, Sr, Th, Ti, Tl, U , V , Zn and Zr, in the blood and urine sam ples from the general population in Sanm en County of Zhejiang province, a typical coastal area of eastern China. Methods The 33 elem ents in 272 blood and 300 urine sam ples w ere determ ined by inductively coupled plasm a-m ass spectrom etry (ICP-MS ). The norm ality test of data w as conducted using SPSS 17.0 Statistics.The data w as com pared w ith other reports. Results The norm al reference values of the 33 elem ents in the blood and urine sam ples from the general population in Sanm en County w ere obtained, w hich of som e elem ents w ere found to be sim ilar w ith other reports, such as Co, Cu, Mn and Sr, w hile As, Cd, H g and Pb w ere generally found to be higher than those previously reported. There w as a w ide variation betw een the reports from different countries in blood B a. Conclusion The norm al reference values of the 33 elem ents in the blood and urine sam ples from the general population in Sanm en County are established, and successfully applied to tw o poisoning cases.

Herpes simplex virus type Ⅰ(HSV-1) is a common pathogen, and human is the only natural host of it.Following a period of lytic replication in epithelial cells, HSV-1 enters axon terminals of sensory neurons and then travels via retrograde transport to the sensory ganglia where latency can be established.Upon the stimulation of some stressors, the latent virus can reactivate, leading to recurrent diseases.Therefore, to clarify the mechanism of HSV-1 latent infection and stress-induced reactivation will offer new insights into the prevention, treatment and control of HSV-1 infection.In this review, we describes the mechanisms underlying HSV-1 latent infection and stress-induced reactivation.

Objective To investigate the vision-related quality of life （VRQL） in patients with glaucoma and to explore the intervention. Methods 260 patients with primary open angle glaucoma (POAG) were investigated with questionnaire of VRQL and self-evaluation before and after health education. 100 health adults with similar age and vision was selected as controls. Results There were significant decrease on VRQL and self-evaluation in patients with glaucoma. Systematic health education can significantly improve their VRQL and self-evaluation. Conclusion The in patients with Glaucoma may result in decrease of VRQL， and systemic health education can improve the quality of life.

Objective To analyze the features of surface electromyography (sEMG) signal of the tibial anterior muscle and gastrocnemius muscle in the stroke patients when sitting and standing.Methods Fifteen stroke patients and fifteen normal subjects were involved in this study. All subjects were asked to stand up and sit down. It repeated five times continuously. The electromyographic signals were collected by surface electrode and then processed by linear time and frequency domain method.Results In sitting position, tibial anterior muscle had significant differences in mean power frequency and median frequency ( P<0.05) when the paretic and non-paretic lower limb, the non-paretic lower limb in stroke patients and in normal subjects were compared. The gastrocnemius muscle had notable differences ( P<0.05) in root mean square when the non-paretic lower limb in stroke patients and in normal subjects were compared. In standing position, tibial anterior muscle also had significant differences in root mean square and integrated electromyography ( P<0.05) as the same frequency domain comparing as above. The gastrocnemius muscle had notable differences on time domain, when the paretic and non-paretic lower limb, the paretic lower limb in stroke patients and the limb in normal subjects were compared ( P<0.05 or P<0.01).Conclusion sEMG is a brief, applied, feasible assessment method in rehabilitation.

Objective To evaluate the feasibility and efficiency of transthoracic echocardiography(TTE) combined with real-time three dimensional echocardiography(RT-3DE)in guiding transcatheter closure of atrial septal defect(TCASD)without X-ray.Methods Eleven patients with atrial septal defects(ASD)underwent the procedure of TCASD guided by TTE combined with RT-3DE.The position of the catheter and transporting sheath,the location and deploying of ASD occluder(ASO)were monitored by muhisection echoscan.especially in RT-3DE.The results were compared with those guided by conventional method(TTE and X-ray).Results All the operations were held successfully.The complications of cardiac tamponade,shedding of occluder,residual shunt,atrioventricular valve regurgitation,embolism and arrhythmia were not observed.Compared with the conventional procedure of the control group,the operation time was significantly prolonged[(65.76±14.15)min versus(45.50±20.88)min,P＜0.000)].Conclusions TCASD was tested to be safe and efficient guided by TTE combined RT-3DE without X-ray,especially for children and pregnant women.It may be expected used widely in the future independently from X-ray.

Objective To investigate the related mechanism of ligamentum flavum (LF) hypertrophy in diabetic pa-tients with lumbar spinal canal stenosis ( LSCS ) .Methods Twenty-four diabetes mellitus patients [ DM (+) ] and twenty normoglycemic patients [ DM (-) ] with LSCS were enrolled in this study .Sorbitol in LF was analyzed using D-Sorbitol/Xylitol test kit .The thickness of LF was measured by CT .The structure of LF was observed after HE and Masson's trichrome staining .The cell cycle and proliferation of fibroblastic cell NIH 3T3 line cultured in high glucose were analyzed .Sorbitol of NIH3T3 was detected under different backgrounds in vitro, normal glucose , high glucose and high glucose burdened with aldose reductase inhibitor ( ARI) , Epalrestat .The expression of inflammatory factors was detected by qPCR and Western blot under above different backgrounds .Results LF of diabetic patients exhibi-ted significantly higher level of sorbitol and pro-inflammatory cytokines , TGF-βand of CD68-positive staining than that of the normoglycemic subjects ( P<0.01 ) .The diabetic LF was significantly thicker than that of the controls , and showed evidence of degeneration .The high glucose-cultured fibroblasts exhibited significantly higher levels of sorbitol , pro-inflammatory factors , and TGF-βcompared to the low glucose-cultured cells , and these levels were dose-dependently reduced by treatment with the aldose reductase inhibitor (P<0.05).Conclusions Sorbitol level of the LF is significantly increased in the DM patients with LSCS .Increased sorbitol recruites inflammatory factors and fibrogenic-related factor TGF-βin LF of DM patients with LSCS which may contributes to the LF hypertrophy .