OBJECTIVE:To establish a method for the content determination of Vincristine sulfate for injection. METHODS:HPLC was performed on the column of Agilent HC-C8 with mobile phase of 1.67% Diethylamine aqueous solution(adjusted to pH 7.5 with phosphoric acid)-methanol(38:62,V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 297 nm,the column temperature was 35℃,and the injection volume was 10μl. RESULTS:The linear range of vincristine sulfate was 0.032-3.2 mg/ml (r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 1%;recovery was 99.3%-101.7%(RSD=0.6%,n=9). CONCLUSIONS:The method is accurate,reliable and sensitive,and can be used for the content determination of Vincristine sulfate for injection.

Objective To assess the clinical effectiveness and safety of gatifloxacin gel to bacterial conjunctivitis. Methods Double-blind and random selection were designed for the study,with levofloxacin gel as the control medicine.Thirty-six eyes of the experimental group and 36 of the control group were eligible for evaluation.Each eye received the gel one drop a time and three times per day.The gels were applied to the conjunctiva sac for 7 d. Results The clinic effectiveness of experimental group and control group were 88.89% and 91.67%,which indicated no significant difference.Microbial eradication rates were 90.48% and 93.75%,no significant difference either.Safety composite scores were similar between groups. Conclusion Gatifloxacin ophthalmic gel is safe and effective for treatment of bacterial conjunctivitis,and contains certain clinical value.

Child ; Common Cold ; diagnosis ; therapy ; Communicable Disease Control ; methods ; organization & administration ; standards ; Communicable Diseases ; diagnosis ; therapy ; Humans ; Patient Education as Topic ; Respiratory Tract Infections ; therapy ; Risk Factors ; Severe Acute Respiratory Syndrome ; diagnosis ; therapy ; Virus Diseases ; diagnosis ; therapy

China ; Humans ; Measles ; prevention & control ; Measles Vaccine ; Vaccination

Female ; Hepatitis B ; prevention & control ; transmission ; Humans ; Infant ; Infectious Disease Transmission, Vertical ; prevention & control

Hepatic drug transporters(HDTs),including uptake transporters and efflux transporters, play an important role in drug distribution and elimination in the liver. The influx HDTs include organic anion transporters,organic cation transporters,organic anion transporter polypeptides and Na+-tauro-cholate cotransporting polypeptide. The efflux HDTs include multidrug resistance proteins,multidrug resistance-associated proteins,breast cancer resistance protein and bile salt export pumps. Major research methods involve gene knock-out animals,isolated perfused livers,liver slices,primary hepato-cytes and transfection cells. They have been used in study of drug distribution,drug-drug interaction and drug toxicity.

Subject-oriented and specialization information service is a new service model adapting to new information environment and catering to demands of customers. On the basis of the practices and researches of subject-oriented and specialization information service in Guang'anmen hospital, this article put forward that providing the information service has positive significance for accelerating the construction of information resource, improving the level of information service and enhancing the core competitiveness of hospitals.

Objective: To summarize the clinical experiences and effect of laparoscopic cholecystectomy(LC)combined with laparoscopic appendectomy(LA). Methods: The clinical data of 92 cases which successfully underwent three-trocar LC and LA was retrospectively analyzed. Results: All operations were performed successfully without complications. The mean operation time was 70 min. The mean hospital stay was 5 days. No complications occurred in the 85 cases who were followed up in 6 months. Conclusion: The simultaneous approach to LC and LA is a safe and effective treatment.

Hand, Foot and Mouth Disease ; epidemiology ; prevention & control ; Humans

Under the characteristics of pathological change of children:insufficiency spleen,excess liver,easy to show liver wood attacking spleen earth,teacher Rong applies the method supporting earth to inhibit wood to children diseases of respiratory system,digestive system,nerve system and others,with definite cure effect.