Forty-one type 2 diabetic patients with fasting plasma glucose≥15 mmol/L and postprandial plasma glucose≥16.8 mmol/L underwent short-term intensive insulin treatment (IIT). Glucose load tests were performed before and after treatment in these patients and proinsulin and C peptide were assayed by RIA. The results showed that IIT decreased the levels of fasting and postprandial proinsulin and improved the ? cell function.

Objective To evaluate effects of two different flap designs (envelope flap and triangular flap) on complica?tions after the mandibular third molar surgery. Methods A randomized, self controlled clinical trial design was selected for 52 patients treated in the outpatient surgery of Stomatological Hospital Affiliated to Tianjin Medical University. Patients were treated with envelope flap design for lower third molar removal in one side and triangular flap on the other side. VAS scores were used to evaluate postoperative pain. The postoperative swelling was evaluated by patient`subjective index. The degree of the upper and lower incisor distance was used to evaluate trismus. Data of postoperative swelling, pain and trismus were re?corded 1, 2 and 7 days after surgery. Data of postoperative wound dehiscence, bleeding situation and alveolitis were also re?corded and compared between two groups. Results There were no significant differences in postoperative pain after 1, 2 and 7 days between two flap designs (P>0.05). After 1 and 2 days there was more severe facial swelling in triangular flap group than that of envelope flap group (Z=2.005, Z=2.017, P<0.05). There was more limited mouth opening after 1 and 2 days in triangular flap group than that of envelope flap group (t=2.578, t=2.410, P<0.05), but on the seventh day there was no significant difference in the severity of facial swelling and trismus between two groups (P>0.05). There were no signifi?cant differences in postoperative pain, alveolitis, bleeding and wound dehiscence between two groups (P > 0.05). Conclu?sion The envelope flap is more conductive to the early recovery in patients after surgery, but in the long term there is no ob?vious difference between the two flap designs.

The reproductive toxicity of environmental endocrine disruptors has attracted substantial attention from researchers in recent years. Bisphenol A (BPA) is among the most prominent environmental estrogens worldwide, demonstrated to be related with the impairment of male reproductive function as well as other health problems, such as diabetes, obesity, cardiovascular diseases, and cancer. BPA acts primarily by mimicking antiandrogenic and estrogenic effects, disturbing the hypothalamic-pituitary-testicular axis and modulating gene expressions and enzyme activities in the hormone biosynthesis affecting steroids or its receptors. BPA is also involved in DNA methylation and the effects of epigenetics, resulting in dyszoospermia, oligoasthenoteratospermia/azoospermia and/or infertility in males. This review addresses the effects of BPA on male reproductive function, focusing on the mechanisms of its toxicity on spermatogenesis, semen quality, and the reproductive system.
Benzhydryl Compounds ; toxicity ; Endocrine Disruptors ; toxicity ; Endocrine System ; drug effects ; Environmental Pollutants ; toxicity ; Estrogens ; toxicity ; Humans ; Infertility, Male ; chemically induced ; Male ; Phenols ; toxicity ; Semen Analysis ; Spermatogenesis ; drug effects

Bone Morphogenetic Proteins ; therapeutic use ; Calcitriol ; analogs & derivatives ; therapeutic use ; Electric Stimulation Therapy ; methods ; Humans ; Osteogenesis ; Osteogenesis, Distraction ; classification ; methods ; Transforming Growth Factor beta ; therapeutic use ; Vitamin D ; analogs & derivatives

BACKGROUND:Recently, the effects of lumbar facet joint morphology on the onset of degenerative lumbar spondylolisthesis have been a discussion point. However, the discussion mainly concerned CT manifestations of joint angle and osteoarthritis. Studies have shown that the changes in cross-sectional area of paraspinal muscle were considered as the essential marker for the pathological diagnosis of degenerative lumbar spondylolisthesis. However, previous studies limited the accuracy of the conclusion due to smal sample size, young age, and measurement.
OBJECTIVE:To observe changes in MRI images of bilateral paraspinal muscles after degenerative lumbar spondylolisthesis.
METHODS:This study adopted the method of retrospective study, selected 80 cases of degenerative lumbar spondylolisthesis as lumbar spondylolisthesis group, and 80 healthy persons as control group. Image J software was utilized to measure cross-sectional areas of bilateral erector spinae and psoas major muscle on the L 3-5 vertebral endplate levels using T2-weighted MRI in both groups. The ratio of relevant data was calculated.
RESULTS AND CONCLUSION:Compared with the control group, the ratio of cross-sectional area of paraspinal muscle was increased on the same side and the same level in the lumbar spondylolisthesis group (P<0.05), and the mean value of cross-sectional area of bilateral psoas major muscle was decreased on the same level (P<0.05). No significant difference in the mean value of cross-sectional area of left and right erector spinae was detected on the same level (P>0.05). Results indicated that the changes in cross-sectional area of paraspinal muscle could induce degenerative lumbar spondylolisthesis, and could be considered as a diagnostic standard for degenerative lumbar spondylolisthesis.

Objective To investigate the proliferation and the change of antigen on the cord blood CIK cells. Methods Cord blood CIK cells were generated by culture cord blood mononuclear cells in the presence of ?-IFN on day 0 and rhIL-2, antiCD3 MCAb on day 1. CIK cultures were analysed on different time point by FACS(Fluorescence activated cell sorter). The killing efficacy in tumor cell lines and primary leukemia cells were detected by MTT assay. Results After 2~3 weeks of culturing cord blood CIK cells expanded about 64.14?16 folds and CD+3 CD+56 cells which were the major cytolytic effector in CIK cells proliferated about 900 folds. Cord blood CIK cells have great cytotoxity against tumor cell lines as well as primary leukemia cells. Conclusions Cord blood MNCs can be cultured to CIK cells and the times of expansion are very high. Cord Blood CIK cells are highly efficient cytolytic effector cells.

Objective To explore an alternative strategy of ganciclovir-mediated cytotoxicity and study the mechanism of enhancing bystander effect of HSV-TK/GCV system by combination gene transfer of Rb gene and HSV-TK gene.Methods Recombinant eukaryotic expression plasmid pcDNA3.1-Rb harbour ing1.65kb of Rb gene was constructed.The pcDNA3.1-Rb and the plasmids tgCMV/HyTK car-ry ing HSV-TK gene were transfected respectively or co-transfected into the osteosarcoma cell line OS732by lipofection using DOTAP reagent.The mRNA expression of Rb gene and HSV-TK gene were detected by RT-PCR and Northern blot.Cell counting,cell cycle analysis and soft agar colony formation test were adopted to observe cell growth features.The expression of gap junction Connexin43gene was performed by RT -PCR and Western blot.The direct confirmation of gap junction intercellular commu ni cation was demonstrated by Lucifer yellow dye transfer.Cell sensitivity to GCV and″bystander effect″of HSV-TK/GCV system were measured by MTT assay.Results The eukaryotic expression plasmid pcD NA3.1-Rb was constructed successfully.The transfected cell lines OS732TK,OS732Rb and OS732RbTK were har-vested.mRNA expression of HSV-TK gene was demonstrated in OS732TK and OS732RbTK cells.Both exogenous and endogenous Rb gene expression could be detected in OS732Rb and OS732RbTK cells si-multa neously.Compared with parental cell OS732,OS732Rb and OS732RbTK cells changed their mor -phology and decreased the growth rate;the ability of soft agar colony formation reduced and the cell cycles were arrested at G 0 G 1 checkpoint.The Connexin43expression and gap junction in tercellular communica-tion en hanced in OS732Rb cell.GCV was of toxicity to only TK -positive cells,OS732TK and OS732RbTK,in a concentration dependent manner,the mixed coculture experiments of OS732and OS732Rb with the TK-negative cell,both showed sensitive to GCV,but the survival rate of OS732Rb cell was significantly lower than OS732cell under the same condition.Conclusion Coordinate ex pression of Rb andHSV-TK gene conferred a more po tent bystander effect by enhancing gap junction intercellular communica-tion and in hibiting prolifera tion.

Objective: To investigate the rule of the alignment of the major and minor segments of the maxillary alvedar bone in infants with comp lete unilateral cleft palate (CUCP).Methods: 30 models of CUCP were used in the study,Markers on the model were designed.The lenth,width, midline and the malposition of minor and major segments on both sides of the cle ft were measured and analyzed.The models of 9 normal infants were employed as th e controls.Results: In CUCP group,the width of alvelar arc h was found significantly larger ( P

Objective To observe the correlation between the C-myc expression and pathologic lesions of kidney to explore the role of C-myc re-expression in children's primary nephrotic syndrome(CPNS). Methods The C-myc expression of renal tissue in 30 cases of CPNS was detected using immunohistochemical method, and the correlation between C-myc expression and renal pathological lesions was analyzed. Results There were various degrees of C-myc positive staining in the renal tissue of all patients with CPNS, while no C-myc positive expression in the renal tissue of control group. C-myc expression was mainly located in podocytes and less in endothelial cells of glomeruli. There was high level of C-myc expression in nephric tubules, especially in proximate tubules. There was no C-myc expression in the Henle's loop,tubulous matrix and vessel areas. There was no obvious difference in C-myc expression level in the podocytes and proximate tubules among the different pathological types of CPNS. The level of focal segmental glomerulosclerosis(FSGS) in proximate tubules obviously reduced compared with mesangil proliferative glomerulonephritis(MsPGN) and minimal change disease(MCD)(P

To study the mechanism and influence of pneumonia on myocardium in D-galactose(D-gal) treated mice, 36 normal male NIH mice were randomly selected and pretreated with D-gal, and then they were divided into three groups, namely control group, the first day after pneumonia group, and the third day after pneumonia group. Another group of 36 mice were pretreated with normal saline to form a general control group. The result showed that in the D-gal prtreated pneumonia mice, the degeneration of myocardial mitochondria was more pronounced, the content of MDA in myocardium was increased, and the activity of SOD and the content of ATP in the myocardium were reduced. There was a positive correlation between the content of ATP and the activity of SOD, and there was a negative correlation between the content of ATP and MDA in the myocardium. These results suggusted that the myocardial lipid peroxidation in the D-gal treated mice increased the susceptibility to the damaging factors, such as pneumonia.