Objective:To investigate the effects of siRNA-mediated Plk1 depletion on COS-7 cells and the therapeutic potential of Plk1 in cancer treatment.Methods:Small interfering RNA(siRNA) were used to specifically deplete Plk1 in COS-7 cells.RT-PCR and immol/Lunoblotting analysis were used to verify the inhibitory effect of siRNA against Plk1.Trypan blue exclusion assay and FACS analysis were conducted to determine the effects of Plk1 depletion on the growth and cell cycle profile of COS-7 cells.Results:Synthetic siRNA duplexes against Plk1 were introduced into COS-7 cells,which subsequently resulted in a significant inhibition in Plk1 expression in the cells.Cell growth assay indicated that siRNA-mediated Plk1 depletion significantly inhibited the proliferation of COS-7 cells.FACS analysis revealed that Plk1 depletion caused cell cycle arrest,resulting into large population of sub-G1 cells.Conclusion:siRNA-mediated Plk1 depletion significantly inhibited the proliferation of COS-7 cells suggesting the therapeutic potential of Plk1 in cancer treatment.

Objective:To investigate the expression and significance of NF-?B,and ICAM-1 in laryngeal carcinoma patients.Methods:Two groups of laryngeal carcinoma tissue (one group derived from the laryngeal carcinoma patients;the other from the normal persons) were detected for NF-kappa B P65 protein and ICAM-1′s expressions by immunohistochemistry staining.Results:(1)The expressions of NF-kappa B P65 protein and ICAM-1 of the laryngeal carcinoma patients were much more strengthened than those of the normal persons(P

Objective:To explore the expression and significance of IKK, I?B and NF-?B P65 of NF-? B signal transduction pathway in laryngeal carcinoma patients.Methods:The expression of IKK?, I?B?,NF-?B P65 was examined by immunohistochemical S-P method in laryngeal carcinoma tissues of 40 cases and normal laryngeal tissues of 20 cases.Results:The expression of IKK? and NF-?B P65 in laryngeal carcinoma tissue was higher compared with normal laryngeal tissue,while the expression of I?B? was lower( P

Acute promyelocytic leukemia (APL) is a subtype of acute myeloid leukemia,which is rare in children.The treatment of APL mainly includes anthracycline-based chemotherapy,all-trans retinoic acid,and arsenic trioxide,which allow for complete remission,improve the long time survival rate and reduce related toxicity as well.About the optimized treatment,quick breakthroughs have already reached,not only at home in the 80's at the end of the 90's,but also abroad,like National Comprehensive Cancer Network of the United States in 2006-2013.This review summarizes the progress in diagnosis and treatment of children with APL.

0.05 ; and (2) in combined test, the combined OR= 3.2353 and its 95% confidence interval was in 2.21 - 4.74 (? 2= 36.2 ,P

Objective To evaluate the association between genetic polymorphisms in promoter regions of matrix metalloprotein‐ase‐1 (MMP‐1)‐1607bp1G/2G ,matrix metalloproteinase‐7 (MM P‐7)‐181‐bpA /G and susceptibility of cervical cancer using Meta‐analysis .Methods Relevant studies that had relationships of the polymorphism in promoter regions of matrix metalloproteinase‐1 (MM P‐1)‐1607bp1G/2G ,matrix metalloproteinase‐7 (MMP‐7)‐181‐bpA /G and susceptibility of cervical cancer were collected as case‐control groups .RevMan5 .0 software was applied to analyze the odds ratios (OR) and 95% confidence intervals(CI) of the as‐sociation between polymorphisms and susceptibility of cervical cancer .Results Finally ,6 studies were selected ,containing 4 studies of MMP‐1 and 2 studies of MMP‐7 .For MMP‐1‐1607bp1G/2G ,OR was 0 .76 (95% CI = 0 .51 - 1 .13 ,P= 0 .17) under 1G/1G +1G/2G ∶ 2G/2G model ,OR was 0 .72 (95% CI = 0 .42 - 1 .23 ,P = 0 .42) under 1G/1G ∶ 2G/2G model ,OR was 0 .79(95% CI =0 .57 - 1 .09 ,P= 0 .15) under 1G ∶ 2G model ,it was concluded that there was no statistical significance between them .For MM P‐7‐181bpA /G ,OR was 0 .47 (95% CI = 0 .30 - 0 .74 ,P= 0 .001) under AA + AG ∶ GG model ,OR was 0 .43 (95% CI = 0 .26 - 0 .74 , P= 0 .002) under AA ∶ GG model ,OR was 0 .77(95% CI = 0 .62 - 0 .94 ,P= 0 .02) under A ∶ G model ,there were statistical signifi‐cance differences under all models .Conclusion Polymorphisms in promoter of MMP‐7‐118bpA/G are associated with the suscepti‐bility of cervical cancer and an allele is a protective factor .It is still not confirmed that significant association between the MM P‐1‐1607bp1G/2G and susceptibility to cervical cancer exists in current literature .

Objective:To test Jiang Guangrong's(2005)three-dimension model on the depth of counseling process.Methods:In this process-outcome research,28 sessions in a university counseling center were videotaped and coded in terms of the model.Structure Equation Model(SEM)was used to examine the relations between the coding and counselors and clients' evaluation of the session depth.Results:(1)Clients and counselors evaluated differently on the session depth,and clients' evaluation was more positive than counselors' [(4.95±1.12)vs.(4.23±0.94),P<0.05];(2)The model was significantly correlated with counselors' session depth evaluation(path coefficient = 0.55,P<0.01),but not with the clients';(3)The session curves describing each dimension changing over time had different patterns in sessions of different depth level,through which more about the counseling process could be known.Conclusion:The three-dimension model on the depth of counseling process has got some empirical evidences.

Objective To study the relationship between CA125 and endometriosis.Methods We tested the level of CA125 in serum and fluid of ovarian cysts of patients with endometriosis and non-endometriosis,respectively.Results The mean CA125 concentration in the fluid of endometrial cysts of the ovary(ECO) was remarkably higher than that of the serum in ECO group(P0.05).Conclusion The level of CA125 has bearing on the occurrence,development and prognosis of endometriosis,CA125 level is positively correlated with the severity of the disease.Therefore,it is valuable to test serum CA125 level for diagnosing endometriosis.

Objective: To investigate the expressions of MMP-2 and MMP-7 between colorectal cancer and normal tissue.Methods: RT-PCR was used to determine the expressions of MMP-2 and MMP-7 in colorectal cancer and normal tissue.Results: The expression rate and level of MMP-2 and MMP-7 were higher in cancerous tissue than that in normal tissue(P

Objective:To study VEGF-C expression in colon cancer and the relationship of VEGF-C expression with clinicopathological features and prognosis.Methods:VEGF-C expression was examined by immnohistochemical staining in 44 cases of primary colon cancers.To analyze the relation of VEGF-C expression with clinicopathological features and prognosis.Results:Of the 44 cases of colon cancers,19 cases of carcinoma (43.2%) were evaluated to be VEGF-C positive expression and 25 cases of carcinoma (56.8%) were determined to be VEGF-C negative expression.VEGF-C expression was associated with tumor differentiation (P=0.003),nodal status (P=0.002) ,Dukes stage (P=0.001).The median survival time of the patients with positive VEGF-C expression was lower than that of patients with negative VEGF-C expression (P=0.0225).Metastatic status,the nodal status and VEGF-C were found to be significant independent prognostic factors (P=0.021,P=0.0004,P=0.0469,respectively).Conclusion:VEGF-C expression is upregulated in colon cancer.VEGF-C expression plays a important role in nodal metastasis.VEGF-C appears to be a new prognostic factor for the prediction of outcome in patients with colon cancer.