To test whether Con A has antirejection effect on mouse islet allograft, 600-700 handpicked islets of C57BL/6 mice was transplanted beneath the kidney capsule of the streptozotocin induced diabetic BALB/C recipients. In control group the mean survival time (MST) of the grafts was 14.1?2.4 days. Treatment with Con A significantly prolong the MST of the islet allografts. In group with Con A treatment of the donor islets prior to the transplantation the MST was 25?5.4 days. The MST increased sigificantly after ip injection of Con A to recipient mice. The combined treatment of islet and recipient with Con A resulted in a prolongation of MST to 25.2?4.4 days. The experiments concerning the mechanisms of the antirejection effect of Con A showed that Con A was able to combine with the islet cell membrane well masking perhaps the histocmpatibility antigen. Furthermore the lymphocytes of mice after i. p Con A injection suppressed the lymphocyte proliferation reaction of the svnergic mice to mitogen.

In this paper, the importance of using advanced medical equipment to check treatment was ana-lyzed, and then pointed out that nowadays there were the following problems: the medical staff had excessive de-pendence on medical equipment; medical equipment inspection treatment of irrationality; medical equipment in-spection treatment with humanity was not enough; medical equipment management of dereliction of duty. Put for-ward to strengthen medical personnel professional moral education;to strengthen the medical students' ethical edu-cation;regular hospital manage concept;improve the cognitive level of medical staff of medical equipment.

Objective To observe the changes of serum 25‐hydroxy vitamin D[25‐(OH)D] level of patients with rheumatoid ar‐thritis(RA) and study its clinical significance .Methods The method of chemiluminescence was adopted to detect peripheral blood serum 25‐(OH) D of 60 cases of RA patients(RA group) and 60 cases of healthy people(control group) .Results The serum 25‐(OH) D level of patients in RA group was(34 .37 ± 8 .87) nmol/L ,while that of the control group was(61 .33 ± 7 .74) nmol/L ;after comparison ,the differences were statistically significant (t= -6 .892 ,P< 0 .05);the serum 25‐(OH) D level of patients in RA group was in negative correlation with RA patient age ,body mass index(BMI) ,time of morning stiffness ,tender joint count(TJC) , swollen joint count(SJC) ,C‐reactive protein(CRP) ,erythrocyte sedimentation rate(ESR) ,visual analog scale(VAS) for disease o‐verall pain and health assessment questionnaire (HAQ) and disease activity score (DAS28)(r= -0 .201 ,-0 .255 ,-0 .362 ,-0 .296 ,-0 .273 ,-0 .198 ,-0 .323 ,-0 .356 ,-0 .379 ,-0 .418 ;P<0 .05 in all cases);but had no correlation with course of dis‐ease ,rheumatoid factor (RF) ,anti‐cyclic citrullinated peptide (CCP) antibody (anti‐CCP) and antibody concentration (P> 0 .05) . Conclusion The serum 25‐(OH) D level of RA patients is significantly low ,which might be associated with the RA disease activi‐ty .

Objective To investigate the effects of Ligustrazine on TNF-α-induced TGF-β and CTGF expression in human peritoneal mesothelial cells (HPMCs). Methods HPMCs were isolated from human omenta by trypsin di-gestion. Then, the subcultured HPMCs were divided into control group, TNF-α-induced (1 μg/L) group and TNF-α-induced plus low-, medium-and high-dose Ligustrazine (10, 20 and 40 mg/L Ligustrazine respectively) groups. The viability of HPMCs was measured by MTT assay. RT-PCR was used to detect the expressions of TGF-β1 and CTGF mRNAs in HPMCs. TGF-β1 and CTGF in supernatants were measured by ELISA. Cell protein concentration was measured by trace bicinchoninic acid (BCA) method to validate the ELISA assay results. Results Ligustrazine significantly decreases TNF-α-induced TGF-β1 and CTGF expression in a dose-dependent manner at both protein and gene levels ( P < 0. 05 ). In addition, medium-and high-dose Ligustrazine injection significantly ameliorates the viability of HPMCs inhibited by TNF-α ( P < 0. 05 ). Conclusion Ligustrazine inhibits expressions of TGF-β and CTGF of HPMCs in an inflammatory conditions.

At present,there are many problems in the cultivation for medical students including lack of humanistic education,insufficient healthy personality shaping and innovation education.We made explorations in aspects of updating education concept,enhancing faculty construction,emphasizing basic course teaching and promoting students' abilities so as to cultivate the comprehensive quality of students majored in medical imaging (ultrasonic medicine).

Alteration in gastric emptying may play a role in the pathogenesis of many diseases.And various methods to measure gastric emptying have been developed.Nine procedures for the assessment of gastric emptying were reviewed in this paper including intubation test,radiology method,absorption test,real time ultrasound,breath test,radionuclide technique et al .Among all of these methods,radionuclide technique is considered as the good standard method.

Recent developments on separation and analysis of bacterial cells by capillary electrophoresis are reviewed. Based on bacterial surface characterization, capillary electrophoresis technique can be utilized for rapidly identify and quantify intact bacteria and microbes at a certain phase. The microbe-molecule interactions can also be described. The separation of microbes that can not be cultured in lab appears to be possible by this method. It makes profound effects on separation science and microbiology to establish capillary electrophoresis procedure for the separation and analysis of bacterial cells.

Objective To establish a qualitative and quantitative method for controlling the quality of Radix Puerariae and its preparation. Methods The qualitative fingerprint of effective part (extract part of ethyl acetate) was obtained by using RP-HPLC with methanol-water (42∶58) as mobile phase and UV detection at 250 nm . The quantitative measurement of effective components (puerarin and daidzein) was finished under the above chromatographic conditions. And the 11 kinds of different samples of Radix Puerariae were analyzed by the method. Results Under the qualitative conditions, the 5 common peaks in RP-HPLC fingerprint of the effective part could be used as index peaks for qualitative identification. In RP-HPLC quantitative analysis, the recoveries of the method were 98.0% with RSD of 1.30% for puerarin and 95.4% with RSD of 1.61% for daidzein, respectively. The amount of puerarin and daidzein in Radix Puerariae was 0.213%～3.626% and 0.012%～ 0.049% , respectively. Conclusion The analytical method with qualitative fingerprint of effective part and quantitative measurement of effective components of Radix Puerariae can be accurately used for controlling its quality.

In this work, some interference in the assay of vitamin E in liver and serum was studied and the method was improved.The sample must be protected from oxidative attack. The interference of high vitamin A content in liver could be removed by H2SO4. The amount of KOH for saponification must be in keeping with the amount of sample assayed both in synthetic a-tocopherol and in biological material by fluorometry. A linear correlation of the fluorescence and the vitamin E content was obtained when 0.5ml of 10 N KOH was used for the saponification of 0.5-100?g of ?-tocopheryl acetate.

[Objective]The article summarizes the experience of Professor HE Lingzhi in treating purpura nephritis . [Methods]To collect and analyse the theory of HE's experience of herb pairs in treating purpura nephritis by studying from her for a long time and one medical record. [Results]Professor HE Lingzhi believes that the disease needs stage therapeutics and it is caused by pathogen wind pyretic pathogenic factor and poisonous evil in the stag of onset. Deficiency of both qi and yin in remission period. Syndrome of blood stasis consistently throughout purpura nephritis. Pro. HE treats purpura nephritis by pairing drugs,such as schizonepetae and honeysuckle, caulis sinomenii and saposhnikovia divaricata, astragalus membranaceus and eclipta alba, the root of red-rooted salvia and puccoon. [Conclusion] Professor HE's clinical experience in treating purpura nephritis from pairing drugs is well worth learning for it always gets outstanding effect.