[Objective] The better optimization of traditional Chinese medicine for the treatment of respiratory syncytial virus, in the past five years is re-viewed in this paper, also the related domestic experimental research results, and according to the pathogenesis of RSV, it further summarizes the current domestic research for the main direction of the disease. [Methods] The article from the two aspects of compound Chinese medicine and traditional Chinese medicine monomer, according to the pathogenesis of respiratory syncytial virus section, discusses the experiment in vivo and in vitro related rich research results. [Results] It is found by inducing and summarizing, whether individual or compound traditional Chinese medicine, for respiratory syncytial virus re-search has made great achievement, but with experimental study limited to suppress the virus more value-added; replication and inhibiting inflammatory infiltrates the two big aspects, lacking of deeper side of molecular biology research, making the traditional Chinese medicine in the treatment of respiratory syncytial virus research appear too single, not a broader recognition by the industry. [Conclusion] We need to be sure the safety of traditional Chinese medicine for the treatment of respiratory syncytial virus and curative effect, but at the same time must recognize that because of the complexity of its com-position and design of experiment of traditional Chinese medicine, the irrationality of the existence of many factors, it makes progress with many problems, so we must be aware of their own insufficiency, more in-depth explore its mechanism of action, with al kinds of research direction in the future for us.

Purpose To investigate the CT features of different pneumocystis pneumonia (PCP) types in AIDS as well as the relationship between different PCP types and CD4+ T lymphocyte count,in order to deepen the understanding and improve the diagnosis and treatment of PCP.Materials and Methods The CT examination data of 102 AIDS patients with PCP were retrospectively analyzed,the CT findings were divided into 6 types,and CD4+ T lymphocyte count of the 6 PCP types was statistically analyzed.Results ① The CT findings of PCP were divided into the following 6 types:transparent ground-glass type (42 cases,41.2％),muddy ground-glass type (17 cases,16.7％),solid variant (11 cases,10.8％),cystic variant (6 cases,5.9％),interstitial type (19 cases,18.6％),and mixed type (7 cases,6.9％).② The CD4+ T lymphocyte count of different PCP types was analyzed,and the difference was statistically significant (F=2.672,P＜0.05).Further pairwise comparison showed that the CD4+T lymphocyte count of transparent ground-glass type was higher than that of solid variant and cystic variant (P＜0.05),and there was no significant difference between the other groups (P＞0.05).Conclusion CT findings of AIDS with PCP have certain characteristics,and it is helpful to deepen the understanding of PCP through imaging classification.There is a certain correlation between different PCP types and CD4+T lymphocyte count,and it is helpful to improve the diagnosis and treatment of PCP by analyzing the correlation.

Objective To analyze retrospectively the result of nonspecific mastitis diagnosed by mammography and color Doppler's ultrasonic, and to improve the diagnosis accuracy of nonspecific mastitis as well as the ability to identify with breast cancer.Methods 31 cases of nonspecific mastitiswere examined by mammography and color Doppler's ultrasonic.All cases were punctured by biopsy or surgery, and confirmed by pathology.Results The coincidence rates between mammography and color Doppler's ultrasonic with pathology were 64.5% and 67.7% respectively.The coincidence rate between the combined mammography and color Doppler's ultrasonic with pathology were 87.1%.There was no statistically significant difference between the two separate inspection methods (P>0.05).Conclusion Mammography and color Doppler's ultrasonic have their own advantages in the diagnosis of nonspecific mastitis, and are easy to be confused with breast cancer.Combining mammography with ultrasonography can improve the accuracy of unspecific mastitis diagnosis and the ability to identify breast cancer.

Breast tumor is the most common malignant tumor in female,its incidence is increasing in recent years.With magnetic resonance equipment,image acquisition and processing technology is improved,perfusion imaging studies gradually become the focus of study.The author reviewed MRI perfusion imaging and its application in breast tumors.

Objective To evaluate the method of plasmodium DNA template extraction from dried blood on filter paper . Methods Collectedand tested 47 blood samples of suspected imported malaria patients ,the DNA was extracted and tested by using conventional procedures and PCR ,then count and analyze test results of matched design .Results The Kappa value was 0 .492 when compared filter paper dried blood spots with thick and thin blood smear test results ,and the Kappa value was 0 .686 when com‐pared with whole blood sample .Using the results of thick and thin blood smear and whole blood samples as the gold standard to e‐valuate the sensitivity and specificity of filter paper dried blood spot method respectively ,the sensitivity was 75 .0% ,71 .4% and specificity was 78 .9% ,100 .0% .Conclusion Collected blood samples using filter paper can′t get good results in PCR test ,still needs further research .

Objective To explore the diagnosis and treatment of unsuspected gallbladder cancer (UGC)related with laparoscopic cholecystectomy(LC).Methods From January 1995 to Dece m ber 2008,4520 cases of LC were performed,UGC waS discovered in 15 cases.Clinical data of the 15 cases were analyzed retrospectively.Results Among the 15 cases of UGC,7 were discovered intraoperatively,and 8 were diagnosed postoperatively.Postoperative pathological examination showed Nevin stage I in 4 cases,stage Ⅱin 7 cases,stage Ⅲ in 3 cases,stage Ⅳ in 1 case.LC was carried out in the patients of Nevin stage I.Radical resection of gallbladder cancer was performed on the patients at stage Ⅱ and stage Ⅲ.One patient at stage Ⅳ received extended radical resection.The patients were followed up from 6 months to 5 years,during the period 3 cases were died and 12 cases were still alive,the 5-year survival rate wag 80.0%.Conclusions Most of LC-related UGC cases are early cancer.Frozen section examination should be performed for high-risk groups and suspicious persons,which contributes to the discovery of UGC.LC can achieve the radical purpose of Nevin stage I patients,Nevin stage Ⅱ-Ⅳ requires open radical operation.

Ample evidences have showed that the failure of early regeneration of the central nervous system(CNS) in adult mammals is mainly due to the growth inhibitory factor.Efforts have been made to promote neuron regeneration and neurological function recovery by damaging myelin and removing myelin-associated inhibitors.At present,the Nogo protein associated drugs and gene therapy have become a novel effective way to promote axon regeneration after CNS injury;here we reviews the recent progress on the related issues.

Objective To investigate the protective effects of pharmacological preconditioning with diazoxide, an ATP-sensitive potassium channel opener, against anoxia/reoxygenation (A/R) injury to primarily cultured hippocampal neurons of newborn Wistar rats and the mechanism involved and the effects of diazoxide preconditioning on A/R-induced neuronal apoptosis. Methods Primarily cultured hippocampal neurons prepared by enzymatic digestion of hippocampus isolated from newborn (

Objective To study the dynamic changes of DNA content of the fibroblasts in narrow pedicle subdermal vascular network flap (SVNP) during their surviving. Methods Eighteen pieces of surviving skin in pig back with size of 16 cm?8 cm and pedicle wideness of 4 cm were stained with acridine orange, and their DNA contents were measured with the adherent cell analysis and sorting cytometer 570. The tissue samples were taken at 3, 4 ,5 ,and 7 days after operation from different parts of the flap.Results The DNA content of fibroblast in the proximal part of the flap was slightly changed, those in the middle portion increased from 3rd to 5th day and became normal at 7th day, and in the distal portion progressively increased after operation. The results suggested that the transitional part of the flap and skin graft in the middle portion was most active in function and metabolism, and well survived, but the distal portion of the graft was poor in surviving and had a long repairing period. These indicated that local tissue hypoxia stimulated proliferation of fibroblasts and synthesis of the DNA in a short time, but as time wert by, the proliferative speed and DNA synthesis in the individual cell were heterogeneous, probably because of the degeneration or necrosis of some epidermal cells in the graft. Conclusion The wideness and length of the pedicle as well as its vascular anastomoses should be readjusted to promote the blood circulation of the distal skin graft tissues.

Objective To investigate the effects of different concentrations of propofol on anoxia / reoxygenation injury to primary cultured rat hippocampal neurons. Methods Newborn (