Objective: To understand the sex related knowledge, attitude and practice of senior kindergarten children in Luzhou and its influencing factors, and to provide a scientific basis for comprehensive early childhood sexuality education. Methods: In January 2022, the stratified cluster sampling method was used to select 1 438 large class children and their parents in 24 kindergartens in Luzhou. Results: The eligible rate of sex related knowledge, attitude and practice of senior kindergarten children was 27.2% . About 91.0% of children knew that they should not let others see/touch their private parts, 88.0% of children could correctly name the source of life, 35.1% of children could correctly and completely point out the location of male/female private parts and 11.5% of children believed that girls could not play with toy pistols and 1.2% of children thought boys should not wear skirts. The results of multivariate analysis showed that public kindergarten, girls, ≥6 years old, high maternal education (college/undergraduate or above), parental sexuality education with positive attitude/good behavior were associated with high sex related knowledge, attitude and practice( OR =1.69, 1.74, 1.86, 1.37, 1.32, 1.48, P <0.05). Conclusion Sex related knowledge, attitude, and practice among senior kindergarten children is insufficient, however, knowledge regarding sexual assault prevention and life education is sufficient. It is necessary to pay attention to the role of parents attitudes and behaviors in child sexuality education, especially among boys, children from private kindergartens, as well as those with young age or low maternal education.