Increased research and exploitation of the low-temperature enzymes of antarctic microorganisms have occurred over recent years. There are a lot of study on protease, lipase and amylase, and so on. In antarctic microorganisms while some peculiar enzymes of antarctic microorganisms are still less researched. The recent development of the peculiar enzymes of the antarctic microorganisms was reviewed in this article. The properties of chitinase, dehydrogenas,?-galactosidase,RNA polymerase,DEAD-box RNA Helicase and metalloprotease etc. were introduced in detail, and the prospects of their application were also discussed.

ObjectiveTo observe the clinical efficacy of microinvasive thread embedding plusShe Gan Ma Huangdecoction based on ordinary Western medications in treating chronic obstructive pulmonary disease (COPD) (cold-phlegm obstructing the lung). MethodThe treatment group was intervened by microinvasive thread embedding plusShe Gan Ma Huangdecoction based on ordinary Western medications, while the control group was by the Western medications alone.ResultThe markedly effective rate and total effective rate were respectively 10.0% and 93.3% in the treatment group;the pulmonary function showed significant improvements in both groups.ConclusionMicroinvasive thread embedding plusShe Gan Ma Huangdecoction based on Western medications can produce a more significant therapeutic efficacy than dry Western medicationsin treating COPD (cold-phlegm obstructing the lung) in acute attack stage, and it can simultaneously improve the pulmonary function.

BACKGROUND:With elevated medical levels, limb salvage surgery for limb malignant tumor is valuable day by day. At present, the limb salvage surgery has substituted amputation and becomes mainstream and development direction of present surgical treatment of limb tumors. However, so far, there are no unified surgical indications for proximal femur tumor, which are controversial. OBJECTIVE:To investigate the efficacy of artificial total hip arthroplasty combined with extended resection of tumor sections for proximal femoral bone tumors. METHODS:Patients with proximal femoral bone tumors in the Second Xiangya Hospital of Central South University were selected and divided into control group and observation group, with 30 patients in each group. According to the disease, lesion curettage, aneurysm wal inactivation, autologous and (or) al ogeneic bone, mixed bone graft and bone graft fixation were selected to treat the control group. The observation group patients received extended resection of tumor sections and total hip arthroplasty. The operative time, intraoperative blood loss, hospital stay and joint function of two groups were compared. 2 years later, patients were revisited. Metastasis and recurrence rate, death rate and quality of life of two groups were compared. RESULTS AND CONCLUSION:There was no significant difference in intraoperative blood loss and surgery time between the two groups (P>0.05). However, hospital stay of patients in the observation group was shorter than the control group. The excellent and good rate of recovery of joint function (83%) was higher in the observation group than in the control group (53%). The metastasis and recurrence rate within 2 years after surgery (7%) and death rate (3%) were lower in the observation group than in the control group (30%, 23%). Various indicators of quality of life of patients in the observation group were significantly better than the control group (P<0.05). These results confirmed that artificial total hip arthroplasty in the treatment of proximal femur tumors is safe and effective.

Objective To investigate the association of the SNP in miRNA146A with genetic prediposion and earlier recurrence after resection for hepatocellular carcinoma (HCC).Methods In the casecontrol study including 173 HCC cases,DNA were exacted from cancer tissue embedded with paraffin and were amplificated by PCR,SNP was explored in gene sequence of miRNA146a (385 base pair including extron).The outcome were analyzed with genetic prediposion and clinical features.Result Only hsa-mir-146a rs2910164 was found.The genetype frequence of C/C 、G/G and C/G at rs2910164 gene locus were separately 61 (35.3％),21 (15％) and 86(49.7％) in cases.Compared to G/G genetype,C/C and C/G genetype were danger factor to onset risk of HCC (OR =3.086,95％ CI:1.289-7.390) ; C/G was danger factor to earlier recurrence after resection(OR =8.179,95％ CI:2.248-29.759).Conclusion rs2910164 may be associated with genetic prediposion and earlier recurrence after resection of HCC in Jiangxi hans

Colorectal cancer (CRC) is one of the most common digestive system neoplasms in China. Recently, the morbidity and mortality rates of CRC continue to increase with the improvement of people's living standard and the change of the dietary structure. It was accepted that the etiology of colorectal cancer is a multi-factor and multi-step process. However, the mechanism of CRC is still unclear. MicroRNAs (miRNAs) are a class of -21 nucleotide non-coding RNAs and function as a negative regulator of gene expression after transcription, participating in tumor angiogenesis, tumor cell proliferation and invasion. MicroRNA-21 (miR-21), as an important oncogenic RNA, plays a vital role in the development and progression of CRC. This review summarizes the advances in research on miR-21 in colorectal cancer.

Spinal cord injury(SCI) is a serious nervous system trauma,which may consequently lead to various degrees of paralysis and toilet obstacles,and its disability and the high cost heavily burdened the families and community.Therefore,the research on spinal cord tissue regeneration and the repair after injury has important practical significance.A large number of experimental studies have shown that neurotrophic factor plays an important role in the nerve tissue repair process.This article reviews new research progress about the effects of neurotrophic factor in this field.

Objective To understand the current research status of sentinel lymph node (SLN) biopsy in colorectal cancer. Methods Literatures about the application of SLN biopsy in the field of colorectal surgery were collected and reviewed.Results The results of SLNs biopsy accurately reflected the status of the nodal basin. Focused examination of the SLNs could identify micrometastases that might otherwise had been missed by standard histopathological analysis, thus upstaged this group of patients.Conclusion SLN biopsy represents a new and effective technique to predict the tumor status of regional lymph nodes, which offers a potential alternative to improve the accuracy of tumor staging in colorectal cancer.

Objective: To explore the latent structure of Antonovsky's Sense of Coherence(SOC) Scale(13-items) in China.Methods: Data from 656 Chinese subjects were analysed by EFA(exploratory factor analysis) and CFA(confirmatory factor analysis).Results: As a global construct,Antonovsky's theory that an individual's SOC is composed of meaningfulness,comprehensibility and manageability was supported.Conclusion: On the whole,Antonovsky's Sense of Coherence(SOC) Scale(13-items) was suitable to Chinese subjects,however several items of the scale were found to be unfit to the Chinese subjects,and revision of them should be done in further study.

Some published research reports on phthalate esters (PAEs) in the municipal sewage sludge were reviewed in the present paper, and aimed at the problems in the field, some preventive measures and the related research emphases were put forwarded also.

Rat lens aldose reductase (AR) activity was assayed in this study with a fiuorometric method. Rat lenses were homogenized in Na, K-phosphate buffer and centrifuged at 19127 ? g, 4℃ for 15 min. The supernatant was reserved as enzyme fraction. The incubation mixture contained 135 mmol KHaPO4-Na2HPO4 buffer (pH 7.0), 100 mmol Li2SO4, 6 ?mol NADPH, 1.0 mmol DL-glyceraldehyde or 10 mmol glucose and 20/ul enzyme fraction. AR activity in diabetic rat lent have not been shown to be significantly higher than that of the con rols. This method can be used properly to screen AR inhibitors in vitro.