1.Expressions of Connexin and Par-3 in the Distal Margin of Rectal Cancer after Ultra-low Anterior Resection
LIU JUN ; ZHANG WEIKANG ; LIU JINLIN ; LU XIAOMING ; LONG YAOPING ; ZHOU YANCAI ; LIU SHENGHONG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2009;29(3):330-334
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2. A Study on the establishment of immune thrombocytopenia model induced by anti-platelet GPⅠbα antibodies
Kangxi ZHOU ; Rong YAN ; Mengxing CHEN ; Jun LIU ; Qingya CUI ; Renping HU ; Yancai LIU ; Yang ZHANG ; Changgeng RUAN ; Kesheng DAI
Chinese Journal of Hematology 2017;38(5):390-393
Objective:
To establish primary immune thrombocytopenia (ITP) animal model induced by anti-platelet membrane glycoprotein GPⅠbα antibodies AN51 and R300.
Methods:
Twenty guinea pigs (6-8 week) were divided into 4 groups. Five guinea pigs in each group were intravenously injected with different doses of AN51 (0.05, 0.1, 0.2 μg/g) and 0.2 μg/g IgG as control. The whole blood was collected from inner angular venous plexus. Platelets number was determined by an automated cell counter and Swiss-Jim method. Then, the similar protocol was used to establish ITP nude mice model by intraperitoneal injection of different concentrations of anti-platelet GPⅠbα antibody R300, respectively.
Results:
①Five minutes after intravenous injection of AN51 at 0.05, 0.1 and 0.2 μg/g, the platelet counts of guinea pigs reduced about 0-5%, 50%-60% and 70%-80% compared to the control group, respectively. The difference was statistically significant (