OBJECTIVE:To improve the quality standard for Jingwu oral liquid.METHODS:The qualitation of Radix Polygoni Multiflori preparata and solomonseal rhizome in Jingwu oral liquid were identified by TLC and the assaying of 2,3,5,4’—tetra—hydroxyl diphenyl ethylene—2—O—?—D—glucoside in the oral liquid were determined by HPLC.RESULTS:In the TLCs of Radix Polygoni Multiflori preparata and solomonseal rhizome,the color of speckles was the same as that of the control articles in the corresponding places.The linear range of 2,3,5,4’—tetra—hydroxyl diphenyl ethylene—2—O—?—D—glucoside was 0.0 562?g～0.6 744?g(r=0.9 996);The average recovery was 100.1%(RSD=1.89%).CONCLUSION:The method is simple,fast,and reproducible and of high recovery rate,which can be used as quality control for Jingwu oral liquid.

Objective To explore the correlation of serum ferritin (SF),fibrinogen(FIB) and D-dimer levels in patients with small vessel disease(SVD).Methods 359 patients with SVD were selected,including 186 patients with lacunar infarction ( LI) and 173 patients with leukoaraiosis ( LA) .176 healthy controls were enrolled in this study as control group.The SF,FIB and D-dimer concentrations were measured by radioimmunoassay method and Clauss method respectively .Results The serum levels of SF , FIB and D-dimer in SVD patients were significantly higher than those in the controls [(171.82 ±156.28)μg/L vs (100.37 ±70.11)μg/L,(3.27 ±0.93) g/L vs (2.97 ± 0.88)g/L,(1.10 ±1.06)mg/L vs (0.83 ±0.55)mg/L](all P<0.05).Compared with LI subgroup,the levels of SF,FIB and D-dimer were higher in LA subgroup [(185.22 ±108.55)μg/L vs (159.36 ±89.69)μg/L,(3.32 ± 0.86)g/L vs (3.23 ±0.99)g/L,(1.29 ±1.17)mg/L vs (1.03 ±0.80)mg/L],but the differences were not signifi-cant(all P<0.05).Multivariate regression analysis revealed that by comparison to the reference quartile ,the odds ra-tio (95%CI) for SVD patients in the higher quartile (SF>151.16μg/L,D-Dimer>1.02 mg/L) were 3.444(95%CI=2.097-5.158,P<0.001) and 1.767(95%CI=1.114-2.803,P=0.016),respectively after multivariable ad-justment.Conclusion The increased levels of SF ,FIB and D-dimer are closely related with SVD .FIB and D-dimer might come to be attractive approaches to prevent the progress of SVD .

Neoadjuvant systemic therapy for breast cancer patients means systemic treatments before operations.Combination chemotherapy,endocrine therapy and targeted therapy before operation can degrade the tumor staging and increase the breast-conserving rates.Neoadjuvant chemotherapy has been accepted as one of the standard therapies for patients with locally advanced breast cancers.In the neoadjuvant setting,the studies about the use of aromatase inhibitors and trastuzumab in selected patients have also made great progresses.

Objective: To observe the effect of peptisorb on the result of intestinal preparation,the change of correlated nutritional index,the fuction of intestine and the immune function after operation in perioperative colorectal cancer patients.Methods: Sixty patients were randomly allocated into two groups. The trial group (n=30) received peptisorb 3days before and 7days after operation. Control group (n=30) received coloclysis treatment.The the level of Alb, globulin,the number of IgG, IgA,IgM, CD3+,CD4+/ CD8+ ,the station of intestinal mucosa and intestine cleaning during operation, nutritional state after operation, immune function, infcetious complications, length of stay were detected.Results: There were no significant difference in the station of intestinal mucosa and intestine cleaning. The index of nutritional and immune in two groups were descend, but the trail group was better(P＜0.01). The number of IgG,IgA, IgM, CD3+ in the trail group were significantly higher than the control group(P＜0.01),the rate of postoperative infectious complications in the trail group was lower than the control group.The trail group aslo showed shorter average length of stay after surgery than the control(P＜0.01).Conclusion: The peptisorb can ameliorate the host immune function and improve clinical outcome,which is a good nutrition support pathway to perioperative colorectal cance patinets.

Sulfotransferase (SULT) enzymes play important roles in the biosynthesis of estrogen.These enzymes catalyze the sulfation of estrogens to form biologically inactive molecules. Several studies have shown that Arg213His polymorphism in SULT1A1 gene may be a risk factor for breast cancer. In addition, a low mRNA expression of SULT has been significantly associated with reduced recurrence and improved overall survival in patients with estrogen receptor (ER)-positive tumors. Furthermore, SULT may be a crucial factor in the response to tamoxifen treatment. As an important part of estrogen synthetic pathways, SULTs are closely re lated to breast cancer.

A high performance liquid chromatographic method with hypersil C_(18) column, 0.020 mol/L potassium dihydrogen phosphate solution(which contains 0.25% triethylamine, pH 5.1)-methanol(20∶ 80, V/V) as mobile phase and fluorescence detection with 280 nm as excitation wavelength and 320 nm as emission wavelength has been developed for the determination of terbutaline sulfate and propranolol hydrochloride in Erythrocyte suspensions. Erythrocyte surface receptor oscillating reacted 1 h with terbutaline in 37 ℃ water bath. Free terbutaline and propranolol were separated from the membrane receptor-binding complex through 1500 r/min centrifugal in 5 min. The linear range was 0.010-3.000 mg/L in Alsever's solutions and the correlation coefficients were 0.9999 and 0.9998. The relative standard deviations(RSDs) of terbutaline and propranolol were 0.8% and 1.2%, respectively. The limits of detection(LODs) of both were 1.00 and 3.00 mg/L. This simple, safety and sensitive method was suitable for the determination of terbutaline and propranolol in erythrocyte suspensions and for the study of receptor-ligand binding.

Forecasting ; Quantum Theory ; Toxicology ; trends

OBJECTIVE:To establish a method for the assaying of total flavonoids in the extracts of Gynostemma and to provide basis for the study of its internal quality.METHOD:The contents of total flavonoids in6kinds of Gynostemma made from Guangxi were determined by UV-spectrophotometry with a detection wavelength at510nm.RESULTS:Good linear re-lationship with absorbability occurred when the concentration range of rutin was0.0092～0.0553mg/ml(r=0.9999,n=6)and its average recovery was100.8%(RSD=2.25%,n=6).CONCLUSION:The method is simple and accurate,of the testing samples,Gynostemma from Guangxi ranked the first in terms of the content of total flavonoids.

Objectives: To observe the clinical effects of nutritional support with protein diet in patients with moderate and severe burns. Methods: 50 cases were randomly divided into treatment group(25 cases) and control group(25 cases). The control group was treated with routine and nutritional support. The treatment group was given additionally with protein diet (80g/d,consisting of Gln 4g and protein 66g). Either oral admistration or tube feeling was chosen according to patient condition. The treatment lasted 21 days. Results: There was no significant difference between the two groups on the levels of Alb、PA、TF and TLC before the management. The level of Alb in the treatment group was obviously changed after the management. The levels of PA、 TF and TLC in the treatment group were increased significantly and had significant differences between the two groups(P

Objective To investigate hepatitis B virus surface antign (HBsAg) quantitative value quantitatively in serum of chronic hepatitis B virus (HBV) infection patients with different clinical stages,at the same time,to explore the correlation between HBV DNA,patient's age and HBsAg quantitative values.Methods Collected 774 cases without antiviral treatment of chronic HBV infection from our hospital,according to the clinical features,divided cases into six groups:chronic HBV carrier group (102 cases),inactive HBsAg carrier group (211 cases),hepativis B virus e antigen (HBeAg) positive chronic hepatitis B group (236 cases),HBeAg negative chronic hepatitis B group (114 cases),HBeAg positive hepatitis B cirrhosis group (52 cases),HBeAg negative hepatitis B cirrhosis group (59 cases).Used chemiluminescence immunoassay particles analysis to determine HBsAg quantitative value in serum in patients,used real-time fluorescent quantitative polymerase chain reaction method to determine HBV DNA quantitative value in patients,and then compared differences among groups.Results HBsAg quantitative value from high to low respectively were chronic HBV cartier group,HBeAg positive chronic hepatitis B group,HBeAg negative chronic hepatitis B group,inactive HBsAg carrier group,HBeAg negative hepatitis B cirrhosis group,HBeAg positive hepatitis Bcirrhosis group,the median quantitative value of HBsAg were [7.80 (6.69-8.32),7.11 (5.42-8.27),6.57 (5.66-7.53),6.38 (4.39-7.40),6.22 (4.84-6.91),6.13 (5.48-7.01)] ; positive correlation was found between HBsAg quantitative value and HBV DNA in HBeAg positive chronic hepatitis B group and HBeAg negative chronic hepatitis B group (r =0.714,0.390,P ＜ 0.01); negative correlation was found between HBsAg quantitative value and age in chronic HBV infection(r =-0.416,P ＜ 0.01) ; Monitored HBsAg quantitative value of inactive HBsAg carriers after the age 40 had important clinical value.Conclusions HBsAg quantitative value is different in the various phases of chronic HBV infection.HBV DNA levels and age are related with HBsAg quantitative value.HBsAg quantitative value should be monitored in inactive HBsAg carriers after the age 40.