Objective To evaluate the efficacy of recombinant human interferon α-2b gel onflooding prevention in loop electrosurgical excision procedure (LEEP) during and after the operation.Method The 152 patients with cervical disease were randomly divided into three groups: group A(recombinant human interferon α-2b gel treatment group), group B (hypophysin treatment group)and groupC(control group). Results The amount of intraoperative bleeding and the amount of post-operative bleedingof the group A was (2.57±1.19) ml and (2.24±1.75) ml, the group B was (3.00±1.49) ml and (45.86±26.14) hal, the group C was (45.62±39.57) ml and (56.90±41.90) ml, respectively. There was no differencebetween group A and group B in the amount of intraoperative bleeding (P>0.05), but significantly less thangroup C (P<0.01). There was no difference between group B and group C in the amount of post-operativebleeding (P>0.05), but significantly much more than group A (P<0.01). Conclusion Recombinanthuman interferon α-2b gel is effective on reducing intraoperative and post-operative bleeding in LEEP.

Adaptive Immunity ; Bacterial Infections ; immunology ; prevention & control ; Biological Evolution ; Epithelial Cells ; immunology ; Gastrointestinal Diseases ; immunology ; prevention & control ; Humans ; Immunity, Innate ; Lymphocytes ; immunology ; Mucous Membrane ; cytology ; immunology ; Receptors, Pattern Recognition ; immunology ; Toll-Like Receptors ; immunology

In the study, the inhibitory effect of epigallocatechin gallate (EGCG) on Calcium oxalate nephrolithiasis and its possible mechanism were investigated. The rat Calcium oxalate nephrolithiasis model was induced through the combined oral administration of ethylene glycol and ammonium chloride, which was intervened with EGCG. Rat blood samples were collected to detect blood creatinine (Cr), blood urea nitrogen (BUN) and blood calcium. Rat urine samples were collected to observe and compare 24-hour urine volume, oxalic acid (Ox) and calcium in urine. Renal samples were collected to prepare tissue slices and observe the pathological changes in Calcium oxalate nephrolithiasis. The expression of osteopontin (OPN) in renal tissues was evaluated by Real-time PCR and Western blot. According to the results, compared with normal rats, rats in the nephrolithiasis model showed significant increases in Cr, BUN, urine Calcium, urine Ox and renal OPN expression (P < 0.05), but obvious decrease in 24-hour urine volume (P < 0.05); Compared with rats with nephrolithiasis, those processed with EGCG revealed remarkable declines in Cr, BUN, urine Calcium and urine Ox (P < 0.05), with significant rise in 24-hour urine volume (P < 0.05) in a concentration-dependent manner. Additionally, compared with the control group, nephrolithiasis rats showed significant pathological changes in Calcium oxalate calculus. After ECCG treatment, the renal pathological changes and OPN expression attenuated significantly in a concentration-dependent manner. The results showed that EGCG inhibits the formation of calcium oxalate nephrolithiasis in rats and shows a notable protective effect on renal functions.
Animals ; Blood Urea Nitrogen ; Calcium ; blood ; Calcium Oxalate ; metabolism ; Catechin ; administration & dosage ; analogs & derivatives ; Creatinine ; blood ; Disease Models, Animal ; Humans ; Kidney ; drug effects ; metabolism ; Male ; Nephrolithiasis ; blood ; drug therapy ; genetics ; Osteopontin ; genetics ; metabolism ; Rats ; Rats, Wistar

OBJECTIVETo observe the effect of Angong Niuhuang Pill (ANP) on Thl/Th2 in cerebral infarction patients and to explore the mechanism of phlegm-heat obstructing orifices.

METHODSRe- cruited were 30 cerebral infarction patients of phlegm-heat obstructing orifices syndrome (PHOOS) both in China and Indonesia. They were assigned to 4 groups according to the use of ANP, the Chinese treatment group, the Indonesia treatment group, the Chinese control group, and the Indonesia control group. Patients in the two control groups received conventional treatment, while those in the two treatment group additionally took ANP for 30 successive days. Their adverse reactions were observe, and levels of INF-γ and IL-4 were detected.

RESULTSThe INF-γ level and the INF-γ/IL-4 ratio significantly decreased, and the IL-4 level increased after treatment in the four groups with statistical difference (P < 0.05). Compared with the control group after treatment in the same country, the INF-γ level and the INF-γ/IL-4 ratio were lower, and the IL-4 level was higher in the two treatment groups (P < 0.05). Compared with the two Chinese groups, the INF-γ level and the INF-γ/IL-4 ratio were higher, and the IL-4 level was lower in the two Indonesian groups (P < 0.05). There was no statistical difference in the post-treatment indices between the two treatment groups.

CONCLUSIONSANP had moderating effect on Th1/Th2 in cerebral infarction pa- tients. Cerebral infarction patients of PHOOS might exist certain relation with Th1/Th2.

Cerebral Infarction ; drug therapy ; China ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Hot Temperature ; Humans ; Indonesia ; Interferon-gamma ; metabolism ; Interleukin-4 ; metabolism

Objective To investigate the role of matrix metalloproteinase 2, 9 (MMP 2, MMP 9) and tissue inhibitor of matrix metalloproteinases 2, 1(TIMP 2, TIMP 1 ) in human amniochorionic membrane Methods Amniochorionic membranes were collected from the following groups of women: (1) women with spontaneous premature rupture of membrane (PROM) before onset of labor (PROM group, n =8), (2) Women with term labor after vaginal delivery (vaginal delivery group, n =8), (3) Women undergoing elective cesarean section (C section group, n =8) Messenger ribonucleic acid expression for MMP 2, MMP 9, and their specific inhibitors TIMP 2 and TIMP 1 were studied with reverse transcriptase polymerase chain reaction Results (1)MMP 2 level in PROM group was 0 849?0 037;in vaginal delivery group 0 327?0 023;in C section group 0 307?0 028 Expression in PROM group was highest, with significant difference compared with the other groups ( P 0 05) (2)MMP 9 level in PROM group was 0 026?0 004;in vaginal delivery group 0 008?0 001,with significant difference between the two groups ( P 0 05) Conclusions In PROM group,the expression of MMP 2, MMP 9 and their inhibitors TIMP 2, TIMP 1 is imbalanced, leading to increased extracellular matrix degradation, and weakening of the fetal membranes, and eventually premature rupture of the membranes

room temperature.

Objective:The aim of the present study was to examine the relationships between alexithymia, negative affect,and quality of life(QOL)of lung cancer patients receiving chemotherapy.Methods:51lung cancer patients receiving chemotherapy completed questionnaires of quality of life questionnaire for Chinese cancer patients receiving chemobiotherapy(QLQ-CCC),26-item Toronto alexithymia scale(TAS-26)and symptom checklist (SCL-90).Results:1)The patients had better score in QLQ(increased by 11%,P

Objective To investigate the gene therapy of hypoparathyroidism (HPT) with hemopoietic stem cell s(HSCs) integrated with parathyroid hormone (PTH) gene.Methods Human PTH gene was amplified by polymerase chain reaction from pcDNA 3.1 -PTH templates and inserted into murine stem cell virus (MSCV) vectors. The plasmids recombinated with PTH gene were transfected into PA317 packaging cells and screened for positive clones with Geneticin. The condensed recombinant retroviruses were obtained and used to infect HSCs coming from umbilical cord blood. The infected HSCs were injected into the mice suffering from HPT. The change of symptoms and serum level of PTH and calcium in each group of mice was observed.Results Human PTH gene was proved to be inserted into MSCV vectors and the titres were up to 2?10 7 colony forming unit (CFU)/ml in condensed retroviral solution. The secretion of PTH reached 15 ng per 10 6 cells per 48 h. The wild type viruses were not detected by PCR amplification. The mice suffering from HPT recovered quickly and the serum level of calcium and PTH remained normal for about 3 months after the HSCs recombinated with PTH gene were injected into them. In the groups of mice subject to the injection of the recombinant retroviruses containing PTH gene, the serum level of PTH and Calcium was significantly increased in a short time, and then declined slowly. And the mice took on the manifestation of hypoparathyroidism again. The mice only subject to the implantation of HSCs were all dead within 20 days.Conclusion A long term therapeutic effect could be obtained by infusing the HSCs which were integrated with PTH gene into the mice suffered from hypoparathyroidism.

Objective To probe into the remodeling and maturity process along with extension of time in anterior cruciate ligament (ACL) reconstruction with hamstring autograft in order to confirm the date of maturity postoperatively. Methods Thirty-three patients after arthroscopic ACL reconstruction with hamstring autograft, were undergone second-look arthroscopical surgery. Meantime, biopsy specimens were obtained from the mid-zone of the hamstring graft. As a control group, specimens of normal ACL were obtained from total knee replacement of four cases, and specimens of semitendinous tendons obtained from ACL reconstruction of the four cases. The interval from initial reconstruction to second-look arthroscopy ranged from 2 to 36 months(mean 11.9 months). Patients were divided into different groups by postoperative time such as 1-, 4-, 7-, 10-, 13-, 18- and 25- month group. Thirty-three specimens were ordinarily sliced up into longitudinal sections. The sections were stained with hematoxylin and eosin so that cell and collagen fibrils were observed under light microscope, then compared the results with those of control group. Results Under microscopical observation, it was showed that tissue of hamstring graft was remodeled from more blood vessels and cellular amount to normal one, and from an irregular orientated crimp pattern of collagen to regular orientated crimp pattern of collagen in a time-dependent manner. Decreasing cellular amount, changing cell type, and a regular orientated crimp pattern of collagen of the hamstring graft were observed in 7- month group primarily, comparing with the original ACL. However, there existed a slower process in the other groups that are older than that of 7- month. Conclusion There are well histological features in the process of remodeling and maturation of the hamstring autograft after operation following extension of time, such as better survival of early period, and remodel of vessels and collagen. The process of remodeling and maturation in the hamstring autograft is similar to BPB autograft. The maturation period of the hamstring autograft after ACL reconstruction in human patients appears between 7 month and 9 month postoperatively.