AIM: To investigate the effects of c-fos antisense oligoneuleotide and p21 genetic transfection on the intimal proliferation of venous autografts. METHODS: The external jugule veins were autografted into common carotid arteries in the same side in 20 New Zealand rabbits, which were divided evenly into experimental and control group randomly. The transplanted veins of experimental group were immersed in the adenovirus - mediated p21 gene solution for 15 minutes just before anastomosis and coated with c-fos antisense oligoneucleotide glue gel just after anastomosis, while the control was only treated with empty vector. The transplanted vascular sample were taken at 2 weeks after operation. The intimal thickness (IT), degree of restenosis (DR), expression of proliferating cell nuclear antigen (PCNA), quantity of VSMC were determined by immunohischemistry. RESULTS: The IT , DR and expression of PCNA, VSMC were decreased, compared to control group. CONCLUSION: Transfection of c-fos antisense oligoneuleotide and p21 gene inhibits the intimal proliferation of venous antografs. [

Acupuncture Points ; Acupuncture Therapy ; Adult ; Female ; Humans ; Male ; Middle Aged ; Shoulder Pain ; therapy ; Young Adult

Endocrine Disruptors ; Environmental Exposure ; adverse effects ; Environmental Pollutants ; Humans ; Obesity ; epidemiology

The eukaryotic expression vector pREP-8-IL-2 or and pREP-8-IFN-? was injected i.p. into mice by calcium phosphate coprecipitation method and IL-2 gene or IFN-? gene was successfully transfected into peritoneal M?s whose expression products could enhance the cytotoxicity of M?s, which secrete some TNF, IL-1 and NO, activate the nonspecific immunity and inhibit the growth of tumor effectively. In particular, IL-2 gene in combination with IFN-? gene transfection were successfully transfected into peritoneal M?s whose high expression products not only significantly enhance the M?s cytotoxicity and made it secrete high level TNF, IL-1 and NO, but also activated the CTLs of spleen and initiated specific immunity and nonspecific immunity . This would produce synergic antitumor effects. The results showed that IL-2 and IFN-? gene transfection produced more antitumor effects than IL-2 or IFN-? gene transfection alone.

AIM: To analyze single nucleotide polymorphisms (SNP) of primary open angle glaucoma- and metabolic syndrome-related genes in primary open angle glaucoma (POAG), in order to elucidate the roles of metabolic syndrome as a risk factor in POAG progress.METHODS: SNP genotypes and alleles of interleukin-6 (IL- 6), IL- 6 receptor (IL- 6R), dopamine D2 receptor (DRD2), beta-fibrinogen (FGB), peroxisome proliferator-activated receptor-γ2 (PPARG), transforming growth factor-β1 (TGF-β1), E-selectin (E-Sel), apolipoprotein A-5 (APOA5), C-reactive protein (CRP), ectonueleotide pyrophosphatase/ phosphodiesterase 1 (ENPP1), hepatic lipase (LIPC), adiponectin (ADIPOQ), paraoxonase 1 (PON1) and serine protease inhibitor E (SERPINE1) genes in POAG (n= 37) and normal control (n=100) groups were measured with ABI Prism 7900HT Fluorescence Quantitative PCR and TaqMan SNP Genotyping fluorescence probe kit.RESULTS: Genotypes and allele frequencies of IL- 6R, IL- 6, FGB, CRP, ENPP1, LIPC, ADIPOQ, PON1, and SERPINE1 in total POAG group were significantly different compared to the control group. CONCLUSION: Metabolic syndrome as a risk factor for POAG may be associated with genotypes and allele frequencies of the related genes.The corresponding gene expression and function can affect POAG progress, including roles of SERPINE1 in extracellular matrix, ENPP1 in insulin inhibition, IL- 6 in endogenous neuroprotection, IL- 6, IL- 6R and E-Sel in autoimmune response, LIPC and FGB in blood hyperviscosity syndrome, ADIPOQ in NOS/NO production, PON1 in vascular endothelial protection.

Objective To explore the diagnosis and treatment of closed abdominal injuries in infants.Methods A retrospectively analysis of the clinical data of 84 infants with closed abdominal injury was made.Results Among the 84 cases, 60 cases had closed parenchymal visceral injury (32 cases of liver and 28 cases of spleen), 10 cases had small intestine perforation, and 14 cases had soft tissue contusion of abdominal wall. Non-operative treatment was used in 27 cases of liver injury and 22 cases of splenic injury, operative treatment in 5 cases of liver injury and 6 cases of splenic injury, and operative treatment for the 10 cases of other types of injury. All the 84 infant patients were cured.Conclusions In diagnosis and treatment of closed abdominal injuries in infants, abdominal centesis, type-B ultrasound and CT should be rationally chosen and applied in order to quickly determine location and extent of visceral injury; indications for operation should be under strict control. Non-operative treatment of parenchymal visceral injury of infants is proved to be safe and with a high cure rate.

Neovascularization plays an important role in embyonic development and many diseases. VEGFs and angiopoietins are two known growth factor families that are specific to vascular endothelium. The action of angiopoietins is associated not only with angiogenesis but also with postnatal vasulogenesis. Thus there is a good prospective use for angiopoietins or their antagonists to promote or inhibit angiogenesis in clinical therapy.

PNAS-4 is a novel pro-apoptosis gene identified latetly. In recent years, there has been a large number of research reports on the basic studies about PNAS-4 in cancer gene therapy and gene therapy of PNAS-4 alone or combined with chemotherapy or radiotherapy manifested a good application prospect, but its molecular mechanisms to promote apoptosis is not clear yet. In this paper, recent research about PNAS-4 in cancer gene therapy is briefly reviewed, and recent hypotheses on its molecular mechanisms to promote apoptosis are especially elucidated. Based on its newly identified characteristics of structural domain, we made a point that PNAS-4 might regulate functions of some target protein related to apoptosis by deSumoylation as a new deSumoylating isopeptidase, and consequently promote apoptosis.
Apoptosis ; Apoptosis Regulatory Proteins ; genetics ; Genetic Therapy ; Humans ; Neoplasms ; therapy ; Sumoylation

Objective In order to optimize patient's medical treatment processes, and to improve the working efficiency of health care professionals and the quality of medical services. Methods The design and application of the electronic prescription module were introduced, and its advantages and existing problems were discussed. Results The electronic prescription module were applied generally. Orthodox patient's medical treatment processes were changed and the prescription management was normalized. Conclusion The electronic prescription module is one of the core components of the workstation system for the out-patient physicians. It optimizes the patient's medical treatment processes and improves the efficiency and the quality of medical services.

Objective:To choose a best method of estimating the amount of blood loss after cesarean deliveryby comparing of three methods,to analyze the related factors of the amount of the blood loss more than 500ml after cesarean section.Methods:We collected the clinical data of the patients who were accepted cesarean delivery and treated in our hospital between January 2014 and December 2014.We estimate the amount of blood loss by different methods(including visually estimated,hematocrit (HCT) change and formula).Based on the formula method as the gold standard,we analyze the risk factors of the patients whose blood loss were more than 500 ml.Results:Among the 583 patients,using the method of visually estimated,there were 23 cases whose blood loss were more than 500 ml(3.95％),using the method of formula,there were 133 cases whose blood loss were more than 500 ml (22.81％),using the method of HCT change,there were 116 cases whose blood loss were more than 500 ml(19.90％).The incidence of patients whose blood loss were more than 500 ml between HCT change and formula method showed no significant difference(P ＞ 0.05),but the incidence by visually estimated method was less than the two other methods (P ＜ 0.05).We found the value of HCT change was a statistically significant positive correlation with the amount of blood loss more than 500ml.When the blood loss was more than 500 ml,the accuracy and sensitiveness of HCT method were significantly higher than those of visually estimated method(76.69％,87.93％ vs 12.78％,73.91％,respectively).Abnormal labor,uterine leiomyoma,placental factors,and macrosomia were risk factors of cases whose blood loss more than 500ml.Conclusions:HCT method is similar in accessing the blood loss after cesarean section with formula method,especially in the cases whose blood loss was more than 500 ml;HCT method is more convenient than formula method,and more accurate than visually estimated method.HCT method can be used as the standard for estimating blood loss initially,and formula method should be used as the method for estimating the blood loss accurately.Patients with macrosomia and abnormal labor are more likely to occur massive hemorrhage after cesarean section.