Plasma concentrations of cyclic GMP and cyclic AMP were measured by radioimmunoassay in 114 patients with chronic atrial fibrillation, 15 patients with paroxysmal atrial fibrillation and 10 patients with paroxysmal supraventricular tachycardia. The results showed that the plasma cyclic GMP concent rations in all these three groups were significantly higher than those in the control group (21. 45 ?. 01, 23. 10?1. 66 and 20. 91?0. 92nmol/L vs 6. 23?0. 23nmol/L, respectively, P

Objective: To study the related factors of artery occlusion induced cardiac infarction. Methods: The coronary angiography data of 56 patients with total artery occlusion were retrospectively studied. The patients were divided into 2 groups: Group A with cardiac infarction, Group B without cardiac infarction, and the clinical features of the 2 groups were compared. Results: It was found that diabetes mellitus, anginal pectoris, hypercholesterolemia, high and low density lipoprotein, smoking, long term medication, collateral circulation, and changes of electrocardiogram presentation were all related factors of infarction( P 6 months), long term medication, well controlled blood glucose and lipoprotein, and non smoking, probably because these factors can improve the collateral circulation and induce ischemia preconditioning.

Cone-beam computed tomography(CBCT) was used to analyze the anatomic morphology of maxillary canine fossa of different sagittal skeletal patterns patients;implants of various dimensions were virtually placed into the maxillary premolars region.The relationships among maxillary canine fossa and implant were analyzed.The depth of the maxillary canine fossa was(4.33±0.73),(3.77±0.58),(5.18±0.93)mm in the 1st premolar region of class Ⅰ,Ⅱ and Ⅲ respectively.The depth of the maxillary canine fossa was(3.20±0.63),(2.81±0.58),(3.90±0.79)mm in the 2nd premolar region of class Ⅰ,Ⅱ and Ⅲ respectively.There were no significant statistical differences in genders,sides,and loss of tooth.And there were significant statistical differences in three classes(P<0.05).The length of the implants was (10.30±1.70)mm(d=3.3 mm) and (8.77±1.58)mm(d=4.1 mm) in the 1st premolar region and (8.09±1.51)mm(d=3.3 mm),(6.69±1.35)mm(d=4.1 mm) in the 2nd premolar region in patients with teeth and buccal perforation;the length of the implants was (8.98±1.54)mm(d=3.3 mm) and (7.67±1.52)mm(d=4.1 mm) in the 1st premolar region and(7.09±1.59)mm(d=3.3 mm) and (5.79±1.34)mm(d=4.1 mm) in the 2nd premolar region in patients with tooth loss,respectively.There were significant statistical differences in loss of tooth(P<0.05).Analyzing of spatial relationships among maxillary canine fossa and implants of different sagittal skeletal patterns patients especially class Ⅲ patients,by using CBCT has important significance in guiding dental implants.

Objective:To investigate whether plasma lipoprotein-associated phospholipase A_(2)(Lp-PLA_(2)) activity correlates with severity and stability of coronary atherosclerosis and other established cardiovascular risk factors.Methods: Coronary angiography(CAG) was performed in 180 hospitalized patients who were suspected as having coronary heart disease(CHD).The severity of pathological changes of the coronary artery was assessed by the number of diseased coronary branches and Gensini′s score.According to the results of CAG,the 180 patients were divided into 2 groups: CHD group(n=112) and non-CHD group(n=68).The CHD patients were further divided into subgroups according to the clinical types,the number of diseased coronary branches and Gensini′s score.Lp-PLA_(2) activity,white blood cell(WBC) count,high sensitive C reactive protein(hsCRP),lipids,blood pressure and body mass index were measured.The age,sex and prior medical histories including hypertension,diabetes mellitus and smoking status were obtained before CAG in all patients.Lp-PLA_(2),WBC count and hsCRP were compared statistically between the subgroups,and correlation coefficients of Lp-PLA_(2) activity with WBC count,hsCRP and other conventional risk factors for CHD were calculated.Results: Plasma Lp-PLA_(2) activity in CHD patients was significantly higher than that in controls(P

Objective:To analyze the clinical characteristics and laboratory examinations of hospitalized children with influenza, and improve medical personal awareness of severe influenza.Methods:Data of inpatients with influenza from November 2018 to February 2019 at Shenzhen Children′s Hospital was collected, and the clinical characteristics and laboratory examinations were statistical analyzed.Results:Of the 702 cases, 464(66.1%) cases were mild influenza, and 238 cases(33.9%)were severe influenza; the median age was 3.8 years, and 457(65.1%) cases were 0 to 5 years old.The number of patients with basic diseases and wheezing symptoms in the severe group was more than those in the mild group, with statistically significant differences(all P<0.05). The white blood cell count, percentage of neutrophils, neutrophils to lymphocytes ratio(NLR), C-reactive protein and procalcitonin in the severe group were higher than those in the mild group, while the percentage of lymphocytes was lower than that in the mild group(all P<0.05). Logistic multivariate regression analysis showed that underlying diseases, wheezing and NLR were risk factors for severe influenza.The area under the curve of NLR for severe influenza was 0.574, whose best cutoff value was 1.43. Conclusion:Influenza virus infection among children is mainly influenza A, and mostly affected children are under 5 years old.The blood routine of children with severe influenza mostly showes increased white blood cell, mainly neutrophils increased significantly, and increased C-reactive protein and procalcitonin.Children with underlying diseases, wheezing symptoms during the course of the disease, and elevated NLR are more likely to progress to severe cases, and NLR cannot be used as a independent predictor of severe influenza.

Aim To investigate the antidepressant effect of imperatorin(IMP) and explore its mechanism. Methods The model of depression was established by prenatal stress (PS) on late pregnant mother, then male offspring rats were randomly divided into five groups: control, PS model, fluoxetine group(5 mg • kg"1), IMP( 15, 30 mg • kg"1). Sucrose preference test, forced swimming test, and open field test were used to evaluate the behavioral changes. 5- hydroxytryptamine ( 5-HT ) concentration, serotonin transporters(5-HTT) and 5-HT,A receptor(5-HT,AR) mRNA expression in the hippocampus and frontal cortex were measured using an enzyme-linked immunosorbent assay and real-time PCR. Results Compared with PS group, the percentage of sucrose preference and the number of total crossings, center crossings, rearing, grooming and 5-IIT concentration significantly increased in IMP group(/> <0. 05 ,/> <0. 01). The im-mobility time was significantly reduced in IMP group compared with PS group ( P < 0. 01). The 5-HT concentration and 5-HT1A R mRNA expressions of PS offspring rats significantly increased by the administration ofIMP (P<0.05,P<0.01). And, the increased 5- HTT mRNA markedly decreased in IMP group compared with PS group (P < 0. 05 , P < 0. 01 ). Conclusions IMP can obviously improve rat behavior and show anti-depressant effect, which may relate to the 5- HT concentration, 5-HTT and 5-HT,AR mRNA expression in hippocampus and prefrontal cortex.

AIM:To find an efficient and simple surgical procedure of the external dacryocystorhinostomy ( EXT- DCR) in the treatment of chronic dacryocystitis.
METHODS:A total of 270 cases were performed in this retrospective study. A comparison of clinical effects and hemorrhage complications between the experimental group, in which 189 eyes of 167 patients that underwent traditional EXT-DCR without packing, and the control group, in which 121 eyes of 103 patients that underwent traditional EXT-DCR with Vaseline gauze were performed. The postoperative follow-up ranged from 6 to 36mo ( the mean follow-up was 15±3. 6mo).
RESULTS: There were no significant differences in the curative rates between the two groups (97. 9% vs 97. 5%, P>0. 05). But the incidence rate of moderate or severe postoperative nasal bleeding was lower in the experimental group (15. 9%, 30 eyes) than the control group (31. 4%, 38 eyes) and P<0. 01.
CONLUSION: The traditional EXT - DCR without packingis an effective procedure and is more effective in reducing moderate or severe postoperative bleeding when the intraoperative anastomose and the postoperative drainage are well done and the intraoperative bleeding is well controlled.

Objective The recurrence and metastasis of malignant tumor could affect the survival of patients. Early detection of cancer cells in peripheral blood and effective therapy promptly are of clinical importance. The present study was to investigate the carcinoembryonic antigen (CEA) mRNA expression and serum CEA level in blood from patients with colorectal cancer and to evaluate its clinical significance. Methods Peripheral blood samples were collected from 35 eligible patients with colorectal cancer and 9 post operative patients. CEA mRNA was detected by reverse transcriptase polymerase chain reaction (RT PCR) and serum CEA was detected by time resolved fluorometric methods. Results The positive rate of CEA mRNA in blood samples of 35 patients with colorectal cancer was 45.7%, and 2.7% in healthy volunteers. Two of the nine post operative patients (22.2%) showed CEA mRNA positive. The positive rate increases with the pathological staging and showed no significant relation with the malignant extent of cell differentiation. Serum CEA mRNA positive rates in patients with CEA positivity (72.2%) were significantly higher than those in patients with negative CEA mRNA expression (42.3%).Conclusion The expression of CEA mRNA in peripheral blood cells correlates with the pathological staging of colorectal cancer and it could be of potential use to monitor the micrometastases of tumor. Long term follow up is needed to evaluate its clinical significance.

Objective To evaluate the determinants of successful recanalization of chronic coronary arterial total occlusion(CTO) by percutaneous coronary intervention(PCI) as well as the effect of the recanalization on the prognosis of the patients. Methods Fifty-two patients with CTO were treated by PCI. 30 patients (duration more than one month) were successfully recanalized with stent implantation. Their clinical features and prognosis were compared with those without successful recanalization. Results Compared with patients without successful recanalization, patients with successful recanalization had a higher incidence of hypertension(53% vs 23%, P

Objective To evaluate the efficacy of primary percutaneous coronary intervention (PCI) for acute myocardial infarction. Methods 63 patients with acute myocardial infarction were treated by primary PCI within 12 hour from onset and were followed up for one to fourteen months. Results Procedure was successed in 61 patients (96.8%). Three patients received only primary angioplasty and 58 patients received stent implantation. All of them gained TIMI Ⅲ grade perfusion flow. Non-perfusion flow occurred in five patients but their flows were improved by intracoronary urokinase infusion. Ventricular tachycardia and fibrillation happened in five patients, and were converted to sinus rhythm by defibrillation in four and one died. Two of the five patients complicated with cardiogenic shock, with one died on 1st day and the other on 4th day after successful PCI, respectively. Main cardiac events occurred in five patients (17%) during follow-up with two sudden death , one reinfarction and repeated PCI, one received selective CABG and one PCI due to intrastent restenosis. Conclusions Primary PCI could provide higher successful rate of reperfusion with low hospital mortality and good short-term prognosis.