Objective To understand the cognitive status of urology medical staff about hematuria color.Methods The self-designed questionnaire about color cognition was adopted,and the concentration of hematuria samples were determined.The investigation was carried out in 50 health care workers about their cognition of hematuria color.Results The study found that there were differences in color perception between doctors and nurses,doctors and nurses with different titles,doctors and the standard sample,nurses and the standard sample.Conclusions There are differences in judgment and description of the urine sample color in medical personnel,they are more attempted to judge by virtue of work experience and subjective consciousness.Due to the lack of a uniform and objective standards,the medical staff can not accurately and objectively describe the development of patients' condition,thus patients' recovery was affected.Therefore,a unified objective criteria for clinical reference norms of hematuria should be developed as soon as possible to ensure that patients recover quickly and safely.

CENP-Fisacell-cycledependentkinetochoreprotein,whoseexpressionandlocalization patterns are regulated in a cell cycle-dependent manner.CENP-F plays an important role in the development and progress of malignant tumor.The expression of CENP-F is up-regulated in many tumors.It serves as an important marker in cell proliferation.Studies have demonstrated that CENP-F is involved in tumor genesis, progress,invasiveness,relapse and prognosis.CENP-F might be a valuable prognostic marker and potential therapy target in cancer treatment.

Objective To establish a gas chromatography method which will be suitable for determination of one time of the benzene, toluene, dimethylbenzene in the adhesives. Methods DMF was used for dissolving the sample, polyethylene glycol chrom-atographic column, hydrogen flames detector gas chromatography were employed to separate the compounds, determine the nature of serve time, quantify apex area. Results The contents of benzene, toluene, dimethylbenzene in gooey were 7.33-146.50, 7.23-144.50 and 7.21-144.20 mg/L respectively, with a good linear relativity (r=0.999), the rate of recovery were 96.45%-100.09%, 98.45%-100.76%, 97.81%-100.14%, the detection limits of the three chemicals were 0.22, 0.24 and 0.24 mg/L respectively, RSD were 1.60%-2.85% . Conclusion The method is accurate, feasible and suitable for determination of the benzene, toluene, dimethylbenzene in the adhesives.

AIM: To investigate the transfection efficiency of mouse liver with non-viral vector containing manganese superoxide dismutase (Mn-SOD) gene. METHODS: The eukaryotic expression vector, gWiz/Mn-SOD, encoding human manganese superoxide dismutase was constructed. The plasmids of gWiz/Mn-SOD were mixed with cationic lipids, followed by injection into mice via branch of superior mesenteric vein, to induce Mn-SOD over-expression in murine liver detected by RT-PCR, Western blotting, SOD activity and immunohistochemical staining. RESULTS: gWiz/Mn-SOD transfection resulted in the obvious expression of exogenous Mn-SOD mRNA and protein in hepatic tissues at 8 hours after injection, and elevated mitochondria SOD activity 8.4 times in transfected hepatocytes than that in non-transfected cells at 72 hours after injection. It was showed that nearly 70% of mouse hepatocytes was obviously Mn-SOD positive after transfection. CONCLUSION: High expression efficiency of Mn-SOD gene in mouse liver is achieved safely by injection of gWiz/Mn-SOD and cationic lipid mixture into branch of superior mesenteric vein. [

Objective:To study the content of polysaccharide and its antitumor activity in the edible part and the disused part of asparagus. Methods:A orthogonal design of 4 factors and 3 levels was applied to optimize the solvent concentration, solid-liquid ratio, extraction duration and temperature that influencing the extraction efficiency. The difference in the polysaccharides content between the two parts was compared, and the difference in the antitumor activity against MCF-7 cells was observed with SPSS software. Results:The optimum extraction technology was used. The difference in the polysaccharide content between the edible part and disused part of asparagus was significant (P<0. 05). The antitumor activity of the two refined polysaccharides against MCF-7 cells was without nota-ble difference (P>0. 05). Conclusion:There is significant difference in the content of polysaccharide in the edible part and the dis-used part of asparagus, however, there is no significant difference in the antitumor activity in the two parts of asparagus.

Acid-sensing ion channels (ASICs), which are activated by extracelluar H+, are H+-gated cation channels. At present, seven subunits of ASICs family have been identified. Numerous studies revealed that ASIC1a plays important roles in synaptic transmission and plasticity,spatial learning and memory, nociception, as well as cerebral ischemia under physiological and pathological conditions. This review concerns the latest research about ASIC1a to promote the understanding of its physiological and pathological functions.

OBJECTIVE To study the nursing strategies for the complications of peripherally inserted central venous catheter(PICC) used in patients with leukemia chemotherapy.METHODS Seventy-one patients with PICC for anti-leukemic chemotherapy were observed.The indwelling time of catheter varied from 1 month to 8 months.RESULTS Ten patients developed PICC related complications,3 phlebitis,3 infections,3 occlusions and 1 was with bleeding.CONCLUSIONS With the characteristics of easy operation,high safety and long time indwelling,PICC has been widely used in the chemotherapy of leukemia patients.To improve safety,the nusing strategies should on strictly sterile operation,application of correct veins and catheters,careful observation and nursing.

In recent years,the study about the mechanisms of cell apoptosis has been increasingly concerned.Many liver diseases are correlated with caspase family,such as hepatic ischemia/reperfusion injury、inflammation and cancer,etc.In the article,we reviewed the importance of caspase family and the new research on the mechanisms of hepatic cell apoptosis in liver diseases.Meanwhile,we summarized the evidence concerned with apoptosis,and discussed the knowledge of therapeutic strategies.

In recent years, great progress has been made in the pathogenesis of psoriasis, which has provided multiple new targets for the treatment of psoriasis, and ushered the treatment, especially topical treatment, of psoriasis to a new era. Some new targeted topical drugs with action mechanisms different from those of traditional drugs have been applied in clinical practice, which are mainly small -molecule inhibitors targeting cell signaling pathways or enzymes involved in the pathogenesis of psoriasis, such as the Janus kinase/signal transducer and activator of transcription(JAK/STAT) pathway, phosphodiesterase type 4 (PDE4), mitogen-activated/extracellular-signal-regulated kinase kinase (MEK), vascular endothelial growth factor receptor (VEGFR), tropomyosin receptor kinase A (TrKA), retinoic acid receptor-related orphan receptor γ (RORγ), and so on. At present, many phaseⅠ and Ⅱ clinical trials are conducted for these drugs. Some completed trials have shown that they have good effectiveness, safety and tolerance, providing more options for the topical treatment of psoriasis.