BACKGROUND:More recently, stem cel therapy has become an issue of concern. Exogenous neural stem cel transplantation brings new hope for the treatment of nervous system injury by self-replication and differentiation to complement and replace damaged or dead nerve cels.
OBJECTIVE:To explore the therapeutic efficacy of neural stem cel transplantation on Alzheimer’s disease.
METHODS:Thirty APP/PS1 mice with Alzheimer’s disease were randomly assigned into model group, cel solution transplantation group or cel transplantation group (n=10 per group). Another 10 C57BL/6 mice were selected as controls. Embryos of C57BL/6 mice at 18 embryonic days were taken to make neural stem cel suspension folowed by transfection using lentiviral vectors carrying GFP gene at different multiplicities of infection (1, 5, 10, 15, 20). Afterwards, GFP-transfected neural stem cels were implanted into the hippocampus of Alzheimer’s disease mice in the cel transplantation group, while the same volume of complete medium was injected into the hippocampus of mice in the cel solution transplantation group. Morris water maze test was performed at 2 weeks after cel transplantation, and brain tissues of mice was taken and detected histologicaly at 4 weeks after cel transplantation.
RESULTS AND CONCLUSION: Compared with the control group, the escape latency was significantly higher, and the number of crossings over the target quadrant was lower in the other three groups (P < 0.05). Compared with the cel solution transplantation and model groups, in contrast, the escape latency was significantly lower, and the number of crossings over the target quadrant was significantly higher in the cel transplantation group (P < 0.05). Four weeks after transplantation, more intact neurons were found in the cel transplantation group as compared with the model group. These findings indicate that neural stem cel transplantation can improve behavior and morphology performance of mice with Alzheimer’s disease.

Neonatal hyperbilirubinemia is one of the major causes of neonatal hospitalization,which is common in the early stage of the newborn and is characterized by skin and sclera yellow staining.The levels of serum total bilirubin and unconjugated bilirubin increased greatly in children,which may damage the brain function,kidney function,hearing and heart function of the newborn,even cause death.Research has found that hyperbilirubinemia can damage the brain nerve,hearing,kidney and heart of the newborn.The damage degree of target organs is positively related to serum bilirubin level.This article reviews the adverse effects of hyperbilirubinemia on newborns.

Objectives To investigate the specific site for measuring inferior alveolar nerve canal (IANC) by cone beam computed tomography (CBCT),and to provide reference data of the course of the IANC of the crowd.Methods This study was a retrospective data analysis.One hundred and thirty CBCT cases were measured and compared after classification by gender and age (65 men and 65 women,65 middle age and 65 youth).Since the proximal center of the mental foramen area,the cross-section at which the mental hole just completely breaks the buccal cortical bone was defined as S1,and S2,S3,S4 were defined as cross-sections with 1-cm apart from each other since S1.L1,L2,L3,and L4 were measured as the distance from specific sites on the outer wall of the IANC to the alveolar crest,the outer wall of lingual lateral cortical bone,the mandibular minimum margin,and the outer wall of buccal cortical bone.The results were analyzed using independent samples t test,with P＜0.05 being considered statistically significant.Results On the right IANC S2L1,S3L1,S4L1,S2L2,S3L2,S4L2,S1L3,S3L4,the 8 groups of L values measured on these 8 sites,all showed differences between genders (P＜0.05).So did the 10 groups of L values measured on the left IANC S1L1,S2L1,S3L1,S4L1,S1L2,S3L2,S4L2,S2L3,S3L3,S4L4 (P＜0.05).The middle age group and the youth group were statistically different for the data measured on the right IANC S2L4 (P＜0.05).This was the same case for the data measured on the left IANC S1L3.When classified according to gender,L1 in the S1 to S4 sections firstly increased and then decreased,and eventually decreased.The change pattern of L3 was opposite to that of L1.L2 in the S1 to S4 sections firstly decreased and then decreased,and eventually increased.The change pattern of L4 was opposite to that of L2.When the samples were classified according to age,the change pattern L value in the S1 to S4 sections was the same as that under gender classification.Conclusions The effect of gender on the L value of individuals is relatively great,while the effect of age on the L value of individuals value is very small.Setting the corresponding long axis of mandible as a reference,the change pattern of IANC in the mandible can be deduced based on the change of L value from S 1 to S4:from S1 to S4,the course of IANC on the vertical direction is firstly downward,tben turns to upward and goes upward;from S1 to S4,the trend of IANC on the horizontal direction is firstly toward the side of tongue,and eventually turns towards the buccal side.The current study provides reference data for IANC-related anatomy from the mandibular foramen to the mandibular second molars.

Objective To study astragalus polysaccharide′s synergistic enhancement on Carvedilol′s improvement effects on dilated cardiomyopathy′s heart function.Methods SD rat model of dilated cardiomyopathy was established and divided into CON group (no treatment),CAR group (only Carvedilol treatment),APS group (only astragalus polysaccharide treatment) and C+A group (Carvedilol and astragalus polysaccharide treatment) according to their treatment methods.The left ventricular ejection fraction (LVEF),Tei index,high sensitive C reactive protein (hs-CRP),tumor necrosis factor (TNF)-α,interleukin (IL)-1,IL-6,IL-8,CD3+,CD4+ and CD8+ T lymphocyte subsets in four groups were detected and compared.Results The LVEF in C+A group and CAR group were significantly higher than those in CON group and APS group respectively (P<0.05,respectively),while Tei index in C+A group and CAR group were significantly lower than those in CON group and APS group respectively (P<0.05,respectively).The LVEF in C+A group was significantly higher than that in CAR group (P<0.05),while Tei index in C+A group was significantly lower than that in CAR group (P<0.05).The hs-CRP,TNF-α,IL-1,IL-6 and IL-8 in C+A group and APS group were significantly lower than those in CON group and CAR group respectively (P<0.05,respectively),while CD3+,CD4+ and CD8+ T lymphocyte subsets in C+A group and APS group were significantly higher than those in CON group and CAR group respectively (P<0.05,respectively).The hs-CRP,TNF-α,IL-1,IL-6 and IL-8 in C+A group were significantly lower than those in APS group respectively (P<0.05,respectively),while CD3+,CD4+ and CD8+ T lymphocyte subsets in C+A group were significantly higher than those in APS group respectively (P<0.05,respectively).Conclusion Astragalus polysaccharide has significant synergistic enhancement on Carvedilol′s improvement effects on dilated cardiomyopathy′s heart function,which may be related to the enhancement of T lymphocyte immunity and the decreasing of inflammatory reaction.

AIM:To discuss the changes of IL-17, IL-21 in glucocorticoid therapy of active thyroid associated ophthalmopathy (TAO) and analyze the relation with results.METHODS:The 67 patients (134 eyes) of TAO in our hospital, were divided into activity group(32 patients 64 eyes) and atypical activity group(35 patients 70 eyes) according clinical activity score (CAS) grading standard, and 30 cases of healthy as control group at the same time.The activity group were treated by glucocorticoid therapy treatment, and proceed the CAS scores before and after treatment, measured the degree of exophthalmus and width of palpebral fissure.At the same time, compared the expression level of IL-17 and IL-21 in all groups, and analyzed the correlation between the IL-17 and IL-21 and CAS score.RESULTS:Compared with control group, expressions of IL-17 and IL-21 in TAO patients were significantly higher (P<0.05).The expressions of IL-17 and IL-21 in active period TAO patients were higher than atypical activity (P<0.05).After glucocorticoid treatment, the expressions of IL-17 in active period TAO patients decreased significantly(P<0.05), and it was significantly positive correlation with CAS score (before treatment:r=0.8847,P=0.042;after treatment:r=0.8886,P=0.0439) the expression of IL-21 in active period TAO patients was significantly positive correlation with CAS score (before treatment:r=0.8893, P=0.0435;after treatment:r=0.8876,P=0.045).CONCLUSION:IL-17 and IL-21 is closely related to the TAO disease activity, and glucocorticoids impact treatment by reducing IL-17 and IL-21 in activity TAO, IL-17 and IL-21 can be used as one of indexes of predicted curative effect and condition in patients with TAO.

Recently,increasing evidence suggests that gingivitis and periodontitis may be one of the etiological factors for some adverse pregnancy outcomes such as preterm birth,preeclampsia,fetal growth restriction and lateral miscarriage.Although there are some conflicting findings and potential problems regarding uncontrolled underlying risk factors,most of the clinical studies indicate a positive correlation of gingivitis and periodontitis with those complications.This review discusses the relationship and its possible pathological mechanism in order to better prevent the development of adverse pregnancy outcomes.

HL7 is a widely-applied international standard for medical information exchange. With a HL7 data interface applied to a mainstream fully automatic biochemical instrument, this paper implements the information exchange between biochemical instruments and LIS following HL7 standard. This paper can be referred to when HL7 applied in other instruments.

Objective To study the risk factors of type 2 diabetes mellitus patients complicated with cerebral infarction.Methods 32 type 2 diabetes mellitus patients complicated with cerebral infarction(group A) and 30 type 2 diabetes mellitus patients (group B) were enrolled in the study. The living habit, history, blood lipids, blood glucose, body mass index and height were investigated, also common carotid artery intimal thickness (CCA-IMT) and plaque incidence rate were measured by two-dimensional color Doppler ultrasound, and the relationship between them was evaluated.Results There were significant differences between two groups in age, duration of smoking, course of diabetes, high density lipoprotein (HDL) and fasting blood glucose (FBG) (all P

Objective To study the chemical constituents in the immature fruits of Momordica charantia. Methods Isolation and purification were carried out by macroporous absorption resin and silica gel, and compounds were identified and elucidated by spectral and chemical methods. Results Ten compounds were obtained from alcohol extract and five of them were determined as germanicyl acetate (Ⅰ), aglycone of momordicoside Ⅰ (Ⅱ), aglycone of momordicoside L (Ⅲ), charantin (Ⅳ), and ?-sitosterol (Ⅴ). Conclusion For all the first time, compound Ⅰ is found in the plants of Momordica L., compound Ⅱ is found as novel natural product in this plant, and compound Ⅲ is found in China.