Objective:To investigate the inhibition of Eleo-Zedoary solution to human endometrial cancer cell line RL-952 in vitro and its possible mechanism.Methods:Using the MTT assay to display the sensitivity to the drug,the inhibition of Eleo-Zedoary parenteral solution to RL-952 cells in vitro was observed;and using light microscope and transmission electron microscope,the morphological alterations of the cells were observed.Results:The inhibition rate of Eleo-Zedoary parenteral solution to RL-952 indicated both dose-dependent and time-dependent anti-proliferative effect.Microscopically,the large vacuoles in the cytoplasm of the cells were found.Ultramicroscopically,many fat-like drops and obvious swelling of organelles as well as the mitochondrial cristaes in the cytoplasm of cells were observed.In addition the nucleus concentration and the chromatin fragmentation and coagulation,including the apoptotic bodies were observed.Conclusion:Eleo-Zedoary parenteral solution can inhibit the proliferation of human endometrial cancer cell line RL-952 by induing apoptosis and secondary necrosis.

Pharmacy is a professional subject with strong practicality and applicability,in which experimental teaching is an essential part of developping students' innovation and overall qualities. Based on the pharmaceutical experimental teaching status,some reform programs including experimental teaching measures,utilization of online teaching and optimization of evaluation manners were provided on the purpose of improving overall quality of the students and developing more comprehensive students.

Vascular eognitive impairment (VCI) is a large class of syndromes from mild cognitive impairment to dementia, which are caused by the cerebrovascular risk factors (eg, hypertension, diabetes and hyperlipemia), obvious (eg, cerebral infarction and intracerebral hemorrhage) or unobvious cerebrovascular diseases (leukoaraiosis and chronic cerebral ischemia). Accurate early diagnosis provides the best treatment opportunity and slows down the progress of the disease. In recent years, the research on VCI has made a great progress. This article reviews VCI from the aspects of the etiology, clinical manifestation, neuropsychology, imaging, neuroelectrophysiology, biomarkers, and diagnostic criteria.

Mechanisms of UV-B-induced apoptotic regulation in yeast Saccharomyces cerevisiae were studied. The results showed that UV-B irradiation indeed inhibited the growth of yeast cells as well as induced extensive apoptosis during 96 h experiment period. However, survival of 96 h irradiated cells remained 10% while most control cells finally dead after re-growth under UV-B irradiation for 12 d. And by exposed to 0.01 mol/L or 0.1 mol/L H2O2 for 30 min, survival rate of 24 h irradiated cells were 3.0-fold or 3.2-fold than control, respectively. By to heat shock for 30 min or 60 min, survival rate of 24 h irradiated cells were 3.5-fold or 9.0-fold than control, respectively.

?AIM: To explore the application of 25G vitreous cavity perfusion in phacoemulsification for cataract after vitrectomy.?METHODS:Forty-eight patients (50 eyes) with cataract after pars planavitrectomy in our hospital from September 2013 to September 2014 were selected to receive phacoemulsification and implantation of artificial lens with 25G vitreous cavity perfusion. Best corrected visual acuity ( BCVA ) , intraocular pressure ( IOP ) , postoperative discomfort, healing of scleral puncture and postoperative complications were observed.?RESULTS: All patients underwent surgery successfully. Compared to before operation, there was less patients with BCVA ≤0. 1, more patients with BCVA 0. 3-0. 5,>0. 5,>0. 3 (P<0. 05). The visual acuity of 1 eye decreased because of vitreous hemorrhage caused by original fundus lesion recurrence. The mean IOP before operation and at 1, 7d, 6mo after operation were 14. 67±0. 62mmHg, 14.75±0.56mmHg, 14.86±0.58mmHg, 14.88±0.59mmHg, respectively. There were no statistically significant differences of IOP between that before operation and those at 1, 7d, 6mo after operation (P>0. 05). No obvious eye discomfort and severe complications after operation were observed.? CONCLUSION: The effect of 25G vitreous cavity perfusion in phacoemulsification for cataract after vitrectomy is good and it can maintain IOP, keep anterior chamber stable and decrease the complications risk.

Objective To provide references to NSF funding policy and planning based on the grant project analysis.Methods Funding grants of NSF Distinguished Young Scholars in medical field during 2011-2015 were collected,and compared with the data of 2001-2010.Indicator data was analyzed including grant distribution in supporting institutes,geography locations and medical subject.Results In recent 5 years,the number of application for the grant is increasing,while the funding number stays the same.The universities governed by Ministry of Education and China Academy of Science are the primary grantee institutions.Beijing,Shanghai,and other developed areas got more grants than other provinces.Most grants were focused on oncology,neurology and psychology,medical imaging and medical engineering,and pathogen biology and infection.Conclusions The funding number of NSF supporting for Distinguished Young Scholars should be incresed with applications increasing.The supporting covers in areas,institutes and medical subjects should be extensively.

Objective: To investigate the relationship between the serum ferritin concentration and insulin resistance in patients with impaired glucose tolerance(IGT).Methods: The serum ferritin concentration and insulin levels were measured in 40 IGT patients(20 with normal body weight and 20 with obesity),and 40 healthy controls.Blood glucose levels,HbA1C,blood adipose and urine protein concentration were obtained simultaneously.Results: In the IGT patients,the serum ferritin concentration was correlated with Hba1c but not with any other indexes,and it was significantly higher than in the normal controls.Significant differences were observed between the two groups in all the indexes but the fasting insulin level.Conclusion: The serum ferritin concentration increases in IGT patients,which implicates some association of ferritin with glucose metabolism and insulin resistance.

Objective To describe the diagnosis and preoperation evaluation of pancreatic carcinoma with spiral CT three-phase contrastenhanced scan and post-processing.Methods Three-phase helical CT enhanced scan and post-processing images of 41 patients with pancreatic carcinoma were retrospectively analyzed,including the location,size,characteristic of tumor and peripancreatic blood vessels,the results were compared with that of surgery.Results Low degree of enhancement of pancreatic carcinoma in pancreatic phase was showed compared with parenchymal phase.There was significant difference in density between focus and normal pancreas,the lesions were showed clearly.In comparison with surgical results,the diagnostic accuracy of CT was 80.7% and the accurate rate in evaluating the metastasis was 75.8%.Conclusion Three-phase helical CT enhanced scan and postprocessing are of great value in diagnosis of pancreatic carcinoma and it is helpful in choosing the treatment program preoperatively and assessing the respectability of tumors.

Objective To evaluate the academic level and the popularity of Chinese Journal of Laboratory Medicine.Method With Chinese Medical Citation Index(CMCI), we analyzed the amount and distribution of the originals in Chinese Journal of Laboratory Medicine cited by the journals indexed in CMCI.Result The percent of cited papers in all published articles was 55.68% during the period, the average number of cited times is 4.83, the top cited value was 108.206 articles (30.03% of total papers) were cited 5 or more, and the all cited times from them were 2428(73.33% of total cited times). 686 cited articles were written by 526 authors from different countries.The most frequently cited author wrote 12 papers, the authors with one paper cited were 430(81.75% of total authors).The cited authors are from the 31 provinces and other country; Beijing, Zhejiang and Shanghai are in the front of Laboratory Medicine research.Beijing was the top of rank (44.40% of total authors).292 institutes were cited and the most frequently cited institute was Beijing Hospital(10.48% of total cited times).There were 563 citing journals where the self-citing rate is 9.85%.Conclusion The Chinese Journal of Laboratory Medicine has published high quality articles. It is the one of the most important information resource for the laboratory medicine researchers and the most important medical journal.

ObjectiveTo investigate the expression and significance of CDX2,Ki-67 in adenoma and colorectal adenocarcinoma,and the correlation between CDX2 and Ki-67.MethodsThe expression of CDX2 and Ki-67 was evaluated in the colorectal adenocarcinoma,adenoma and normal colorectal mucosa as control subjects by immunohistochemistry.ResultsThe magnitude of CDX2 were negatively correlated with the level of dysplasia in adenoma(rs=-0.38,P =0.02),and negatively correlated with differentiation,lymph node metastasis,diversion and Dukes stage of cancer in colorectal adenocarcinoma(rs=-0.49,rs=-0.37,rs =- 0.38,rs =-0.37,P =0.01).The expression and relativity of Ki-67 in the samples were contrary to the CDX2.The levels of CDX2 and Ki-67 expression had significant difference between the adenoma and adenocarcinoma,and showed negatively correlation in this two type tissues (rs=-0.69,P =0.00; rs =-0.40,P =0.00).ConclusionsCDX2 may be used as an effective marker to evaluate malignancy degree and prognosis of large bowel neoplasm patient.The levels of Ki-67 expression in colorectal adenocarcinoma are correlated with malignancy degree and clinical stage.The expression of CDX2 is negatively correlated with Ki-67.Combined detection of CDX2 and Ki-67 may be helpful for the judgment of colorectal tumor biological characters.