Objective To evaluate the curative effect of ilio-inguinal and Kocher-Langenbeck (K-L) approach internal fixation and reduction in the treatment of acetabular fractures in a floppy lateral position.Methods A retrospective study was done on 31 patients treated from February 2005 to March 2009,including 15 patients with complex acetabular fractures treated with ilio-inguinal and K-L approach internal fixation and reduction in a floppy lateral position ( Group A) and 16 patients with complex acetabular fractures treated with anterior ilio-inguinal approach and posterior K-L approach internal fixation and successive reduction in a floppy lateral position Group B).Operation time,intra-operative blood loss,number of X-ray fluoroscopy times,degree of postoperative reduction,results of Merled' Aubigne and Postel scores as well as the rate of postoperative complications were recorded.Results All the patients were followed up for 15-27 months.In Groups A and B,the operation time was for ( 176 ± 17 ) minutes and ( 196 ± 23 ) minutes ( P ＜ 0.05 ),the intra-operative blood loss for ( 880 ± 16) ml and (874 ± 19) ml (P＞0.05),the number of X-ray fluoroscopy times for 6 ±2 and 14 ±6 respectively ( P ＜ 0.05 ).According to Mata analysis standard,the postoperative fracture reduction was anatomical in 11 patients (73％),good in three and poor in one in Group A; while the postoperative fracture reduction was anatomic in nine patients 56％ ),good in five and poor in two in Group B.The rate of anatomical reduction between the two groups had statistical difference (t =2.731,P ＜ 0.05 ).The modified Merled' Aubigne and Postel score system showed excellence rate of joint function of 93％ ( 14/15 ) in Group A and 75％ (12/16) in Group B,with statistical difference ( x2 =15.292,P ＜ 0.05).There was no statistical difference in the aspect of postoperative complications between the two groups,such as heterotopic ossification,traumatic arthritis,avascular necrosis of the femoral head,nerve injury and so on.Conclusions In a floppy lateral position,combined ilio-inguinal and K-L approach internal fixation and reduction takes advantages of sufficient exposure,good reduction,strong fixation,short operation time and few complications in the treatment of certain complex acetabular fractures,while its long-term effects are worthy of further clinical researches.

Objective To examine the effect of target muscle injection with different dosages of Methycobal in the sciatic nerve regeneration of adult rats so as to determine a better method and proper dosage of Methycobal for clinical treatment of peripheral nerve injury. Methods A total of 30 healthy Wistar rats were employed to make sciatic nerve injury model through immediate suture of the adventitia posterior to transection of the left sciatic nerve. The rats were divided randomly into three groups, ie, high dosage group (Methycobal for 300 ?g/kg, Group A), low dosage group (Methycobal for 100 ?g/kg, Group B) and control group (1 ml normal saline once a day, Group C),10 rats in each group. At 4 and 8 weeks, 5 rats from each group were taken to observe the effect of different dosages of Methycobal on rat sciatic nerve regeneration, where the detection indices included morphological changes and image analysis of sciatic nerves by optic and electron microscopic investigations as well as tricipital muscle wet weight. Results Four weeks after operation, tricipital muscle wet weight was (1.367?0.012) g in the Group A, (1.164? 0.011) gin the Group B and (0.950?0.009) g in the Group C, with significant difference among groups A, B and C (P

Objective To investigate the angiogenesis characteristics of normal breast,simple hyperplasia,atypical hyperplasia,intraductal carcinoma in situ and invasive ductal carcinoma.Methods Twenty cases in normal controls,20 cases with simple hyperplasia,20 cases with atypical hyperplasia,20 cases with intraductal carcinoma in situ and 30 cases with invasive ductal carcinoma were inspected with power Doppler imaging(PDI)and contrast enhanced microvascular imaging(MVI)on the quantity,configuration and distribution of angiogenesis.The microvessel density(MVD)were detected by immunohistochemical technique.ResultsWith the progression of breast cancer,MVD and grades of PDI kept increasing.Meanwhile,the blood vessels became twisted and increased in quantity with expansion into the lesion,the configuration of blood vessels became disordered.However,the changes mentioned above were not obvious in the very early stages.they became significant from the atypical hyperplasia(AH)stage.Conclusions Contrast MVI and PDI may be useful to screen high risk population with high risk of breast cancer due to their detection of changes in angiogenesis of breast carcinoma and precancerous lesions.

Objective To examine the changes of brain inhibitory neurotransmitters and their precursors with glutamine supplement. The hypothesis is that the supplement of glutamine may delay the fatigue of central nervous system involved in exercise. Methods Rats were suffered with exhaustion treadmill running after different dosages of glutamine supplement of twenty days duration and sacrificed right after the running. Rat free tryptophan (F-Trp), branched chain amino acids (BCAA) in serum and brain, glucose in serum, and 5-hydroxytryptamine (5-HT) in the brain had been quantified with the double blind procedure. Results The results showed that the serum glutamine had no statistical significance among each group. The ratios of F-Trp/BCAA in the serum and brain were lower significantly in large dosage group than the placebo group. The 5-HT level of large dosage group was lower significantly than the other groups, while serum glucose was significantly higher than all the other groups. The ratios of F-Trp/BCAA in the brain were lower significantly in small dosage glutamine supplement rats than the other groups. Conclusion The results suggest that glutamine supplement take advantage of the balance of serum glutamine level. Glutamine supplement improves rat central nerves performance in long time treadmill running, that may due to the decrease of precursor of inhibitory neurotransmitter and itself, and large dosage supplement is more effective than small one.

Objective To observe the effect of Yangxue Qingnao Granule on the ultrastructures and expression of p38 mitogen activated protein kinases (p38MAPK) in CA1 area of hippocampus in vascular dementia rats. Methods 180 Sprague-Dawley rats were randomly di-vided into sham group, vascular dementia model group (model group) and Yangxue Qingnao Granule treatment group (treatment group). The vascular dementia was modeled with modified Pulsineli's four-vessel occlusion. The ultrastructure of CA1 area was observed with trans-mission electron microscope, while the expression of p38MAPK in CA1 area was detected with immunohistochemstry and Western blotting 1, 2, 4 and 8 weeks after modeling. Results In the model group, pyknosis, nuclear dissolution, heterochromatin margination and mitochon-dria swelling were found in most of the neurons in CA1. In the treatment group, the distribution of chromatin was well-proportioned, and mi-tochondrion and other organelle were normal. In the model group, the expression of p38MAPK increased at each time point compared with the sham group (P<0.01), and peaked 4 weeks after modeling, and decreased in the treatment group compared with the model group (P<0.01). Conclusion Yangxue Qingnao Granule can improve the ultrastructure of neuronal in CA1 area of hippocampus of vascular dementia rats, which may relate with the inhibition of the expression of p38MAPK.

Objective To study methods and effect of granule bone grafting into the vertebral pedicle pyramid under endoscope for treatment of thoracolumbar fractures. Methods There were 26 cases (7 males and 19 females; age range of 32 79 years, average 51.2 years) with thoracolumbar fractures. The fractures included 8 cases (30.8%) of thoracolumbar compression burst fractures with no compression of the spinal cord by broken bone fragments and 18 (69.2%) compression fractures due to senile osteoporosis. Posterior to a preliminary reduction of the lumbar region on the operating table, all cases underwent vaginal vertebral pedicle reduction under endoscope and autogenous or hererogeneous granule bone graft in order to restore the height of the fractured vertebra pyramid. The patients kept in bed for two months postoperatively; meanwhile, the excise for the back muscle was performed. Results The mean follow up period was 3 18 months. Twenty one cases (80.8%) got excellent therapeutic result, four (15.4%) moderate and one (3.8%) poor. (1) The vertebral height recovered average 29%, with mean height loss of 5% through six month follow up. (2) The posterior convex cobb angle of the spine recovered for 10? 20? (mean 13.8?). (3) No lumbago or nerve compression symptoms were found in 21 cases. Conclusions Granule bone graft into vertebra pyramid under endoscope in the treatment of thoracolumbar fractures is a minimally invasive procedure that can enhance the bone capacity of the vertebra, alleviate pain, restore the height of the fractured vertebra pyramid and accelerate bone healing.

This study was aimed to observe the effect of Yang-Xue Qing-Nao(YXQN) granules on the expression of brain-derived neurotrophic factor (BDNF) and basic fibroblast growth factor (bFGF) in CA1area of hippocampus among vascular dementia (VD) rats.A total of 72 SD rats were randomly divided into the sham operation group,VD model group (model group) and YXQN treatment group (treatment group).The VD rat model was prepared by modified Pulsineli's four-vessel occlusion.The learning and memory abilities of rats were detected by the Morris water maze.The protein expressions of BDNF and bFGF were detected by the immunohistochemical analysis.The results showed that compared with the sham operation group,there was obvious learning and memory disorders in the model group with increased protein expressions of BDNF and bFGF in CA1 area of hippocampus at difference time points (P<0.01).Compared with the model group,the learning and memory abilities of rats in the treatment group were significantly improved; and the protein expressions of BDNF and bFGF in CA1 area of hippocampus were significantly increased at difference time points (P<0.01).The expression was the highest on the 4th week.It was concluded that YXQN granules can improve the learning and memory abilities of VD rats.Its mechanism may be related to the upregulation of protein expression of BDNF and bFGF.

Objective: To understand the current situation of physical exercise participation of primary and secondary school students in Nanjing, and to provide the reference for promoting the healthy development of physical fitness of primary and secondary school students. Methods: By using stratified cluster sampling method, 4 161 students from 14 primary and secondary schools in Nanjing, Jiangsu Province were selected to conduct a questionnaire survey on their participation in physical exercise. Results: Among the primary and secondary school students in Nanjing, 55.57% had more than three hours of physical education class per week, 81.09% of them participated in large class activities organized by the school every day, 23.19% of them participated in extracurricular sports activities more than three times a week, 13.96% of them participated in physical exercise for one hour every day,there were significant in physical exercise items among students of different stages(χ2=52.76, 29.90, 16.14, 39.34; 624.61, 53.04, 151.25, 189.45, P<0.05). 34.34% of the parents supported and accompanied their classmates in family physical exercise, 46.07% of parents supported students’ physical exercise but do not accompanied them, 14.83% of parents’ attitude was not clear, 4.76% of parents did not support students’ physical exercise, 14.32% of families made physical exercise plans; There were significant differences in family physical exercise items among students of different genders and stages(χ2=42.72; 229.44, 26.43, P<0.01). 49.82% of students had more than three sports skills in personal physical exercise, 17.45% of students participated in physical training classes, only 7.14% of students participated in physical exercise weekly. There were genders and stages differences in the current situation of students’ participation in physical exercise(χ2=12.32, 5.60, 24.06; 167.51, 237.90, 223.15, P<0.05). Conclusion In Nanjing, the reporting rate of regular participation in physical exercise among primary and secondary school students is low, and family-school interaction is needed to promote the healthy development of students.

Objective: To understand the current situation and influencing factors of college students’ participation in physical exercise in Jiangsu Province, and to provide reference for promoting the healthy development of college students’ physique. Methods: A questionnaire survey was conducted among 2 643 college students in 12 universities in Jiangsu Province. Results: Only 2.3%(60/2 643) of college students attended physical education classes more than 3 hours a week, 29.9%(790/2 643) of them participated in physical exercise more than 3 times a week, 14.8%(391/2 643) of them participated in physical exercise more than 1 hour a day, and 23.0%(609/2 643) of them mastered more than 3 sports skills. Among the items, 45.7%(1 208/2 643) of the college students did not participate in sports associations, 17.8%(471/2 643) of the college students participated in sports social organizations such as off-campus sports clubs; 40.1%(1 059/2 643) of the students thought that the content of physical education curriculum lacked interest. About 31.9%(845/2 643) of the students did not like the physical education teachers or their teaching methods; 19.4%(514/2 643) of the students thought that they lacked sports venues or equipment; 21.1%(558/2 643) of the students thought that physical exercise had no effect on learning, and 28.9%(764/2 643) of the students thought that physical exercise had no effect on learning. The students thought that physical education curriculum and related activities had no effect on employment. About 17.1%(451/2 643) of the students thought that the performance of physical education did not affect the evaluation and progress; The present situation of college students’participation in physical exercise and the reasons for their dislike of physical exercise are different in gender, grade and region(P<0.05); single factor Logistic regression analysis showed that gender, grade, place and so on. The lack of interesting contents, dislike of PE teachers’ teaching methods, lack of sports venues or equipment were the influencing factors of college students’ participation in physical exercise in Jiangsu Province(P<0.05). Conclusion The current situation of college students’ participation in physical exercise in Jiangsu Province is not optimistic. To promote college students’participation in physical exercise needs the co-operation of society, schools and individuals.

Objective: To understand the current situation and influencing factors of physical exercise participation of rural primary and secondary school students in Jiangsu Province, and to provide reference for promoting healthy development of rural youth in Northern Jiangsu. Methods: A total of 3 597 rural primary and middle school students in Northern Jiangsu Province were selected to conduct a questionnaire survey on the status quo of physical exercise. Results: Only 37.6% of students in Northern Jiangsu had more than 3 hours of physical education every week, 23.6% of the students participated in extracurricular physical exercise for more than 3 times a week, 14.3% of the students participated in physical exercise for more than 1 hour every day, 77.9% of the students master more than 1 sports skills, 72.0% of students participated in extracurricular activities perceived, and 27.3% of the students participated in extracurricular sports professional counseling; 35.7% of the students reported lack of parental support physical education curriculum lacks of interest, 17.0% of the students didn t like the physical education teachers or their teaching methods, 28.6% of the students reported lack of sports venues or equipment, 8.4% of the students reported that their parents do not support, 38.2% of the students reported lack of extracurricular sports professional guidance, 18.1% of the students think that the performance of physical education didn t affect the evaluation and progress of the school. Univariate Logistic regression analysis showed that gender, academic stage, dislike of physical education teaching methods and lack of physical education professional guidance were the influencing factors of physical exercise participation havior of rural students(P<0.05). Conclusion The present situation of physical exercise of rural primary and secondary school students in Northern Jiangsu Province is not optimistic. Special attention to the formation of female exercise behavior, improve the teaching quality of rural physical education and support the off campus sports training institutions.