Objective To investigate the relationship between the autoantibody detection and abortion of pregnant women.Meth-ods The dot immunogold method,indirect immunofluorescence and enzyme-linked immunosorbent assay(ELISA)were used to de-tect serum anti-double stranded DNA antibody (dsDNA),anti-smooth muscle antibody (SMA),antinuclear antibody (ANA),anti-endometrial antibody (EMAb),anti-cardiolipin antibody(ACA),anti-sperm antibody (ASAb),anti-ovarian antibody(AoAb).Re-sults Compared with healthy pregnant women,the values of EMAb,ACA,AoAb,ANA and SMA in abortion pregnant women were increased with statistical differences (P <0.05).Conclusion EMAb,ACA,AoAb,ANA and SMA have high correlation with abortion of pregnant women.Confirming the causes of unexplained abortion and prenatal screening are especially important for the pregnant women in the border area.

The multiple primary carcinoma (MPC) is defined as more than 2 or up carcinomas occured with in same or multiple organs in an individual.Along with the science development and advancement of human society,the life-span of human being has been prolonged,and more of MPC are identified.But the misdiagnosis of MPC as the metastases of primary tumors or missed diagnosis will mislead therapeutic regimens and exacerbate the diseases.Therefore,the accurate differentiation of MPC is becoming increasingly important.This article mainly reviews the epidemiology,diagnostic criteria,etiology,frequent sites of involvement,classification,and diagnostic protocols of MPC.

Objective:To study the structure of teacher’s app raisal of pupil’s self-regulationMethod:Based on the open que stionnair es that teachers provided of their students’ characteristics, a self-regulation questionnaire of primary school students was developed 736 students were asse ssed by 56 teachersResults:Through exploratory factor analysis and confirmatory factor analysis, four factors, independence and conscientiousn ess, assault and resisting, friendship and obedience, irritability and activenes s, were obtained The ideal reliability and validity were gotConclusi on:In the view of teachers, the self-regulation structure of primary sch ool students includes four factors

Objective:To construct an expression vector containing RNA interfering(RNAi) sequence targeting human vitamin D_3 receptor(VDR) protein gene,and to investigate its inhibitory effect on the expression and function of VDR in a human osteosarcoma cell line.Methods: Two small interfering RNA sequences targeting VDR were designed by online screening and were cloned into the expression vector(pSilencer-2.1-U6).Then the products,pSilencer-2.1-U6-VDR1 and pSilencer-2.1-U6-VDR2,were separately transfected into a human osteosarcoma cell line(HOS-8603) by liposome.Western blot was employed to determine their effect on VDR protein expression and 1,25(OH)_2D_3-induced phosphorylation of PKC.Cell counting and MTT were used to evaluate their influence on the inhibitory effects of 1,25(OH)_2D_3 on HOS-8603 cells proliferation.Results: Compared to HOS-8603 cells transfected with control vectors,those transfected with pSilencer2.1-U6-VDR1 or pSilencer2.1-U6-VDR2 had significant decreases in the expression of VDR protein, in the inhibitory effects of 1,25(OH)_2D_3 on the cell proliferation,and in quick phosphorylation of PKC induced by 1,25(OH)_2D_3.Conclusion: The vectors constructed in the present study(pSilencer2.1-U6-VDR1 and pSilencer2.1-U6-VDR2) can significantly suppress the VDR expression in HOS-8603 cells,and block some biological activities of 1,25(OH)_2D_3 mediated by VDR, which paves a way for further study on the biological functions of VDR and vitamin D_3.

Like other mammals,human has obvious circadian rhythm,and light is recognized as the principal circadian synchronizer.Rod/cone photoreceptors of the outer retina and the melanopsin-containing retinal ganglion cells(mcRGCs) of the inner retina mediate non-image forming visual responses including entrainment of the circadian clock to the ambient light,the pupillary light reflex (PLR),and light modulation of activity by means of a set of mcRGCs projecting to the suprachiasmatic nucleus (SCN)through retinohypothalamic tract (RHT).Glaucoma is a group of diseases which cause degenerative changes and loss of RGCs including mcRGCs,so the circadian rhythm of patients with glaucoma will be interrupted.The progress in the study on the effect of glaucoma and experimental hypertension on circadian rhythm was reviewed.

Glaucoma is a common eye disease which may cause irreversible blindness.This ocular degenerative disease affects retinal ganglion cells (RGCs) and eventually causes optic nerve dysfunction by way of axonal loss.Patients with glaucoma may show characteristic changes of optic disc,damages of visual field and impairment of vision.Detection and evaluation of the impairment of visual field in patients with glaucoma are essential for the diagnosis and estimation of progression of this disease.Numerous techniques have been developed to detect field progression,but no standard has been widely accepted.Assessment Methods including visual field indices,glaucoma hemifield test (GHT),Advanced Glaucoma Intervention Study (AGIS),Early Manifest Glaucoma Trial (EMGT),Collaborative Initial Glaucoma Treatment Study(CIGTS),as well as the strength and limitation of each method were reviewed in this article in order to find out suitable ways to evaluate visual field defects in patients with glaucoma in clinical works and researches.

Adult ; Female ; Humans ; Intestinal Obstruction ; etiology ; therapy ; Postoperative Complications ; etiology ; therapy ; Rectal Neoplasms ; radiotherapy ; surgery

Tumor angiogenesis is a complicated pathologic process, which plays an important role in the growth and metastasis of most solid tumors. Targeting at the angiogenesis and inhibiting tumor growth or metastasis, specific antibodies or inhibitors of tumor angiogenesis have been developed for this purpose. We summarized recent researches about the mechanism of tumor angiogenesis and the development of tumor angiogenesis inhibitors in this review.

Refined management calls fordetail decides result,operated in health achievements transformation in hospital,which is a new try.First of all,the author expatiated upon the connotation of refined management in this paper,and then,elaborated the practice of refined management of health achievements transformation in hospital.After that,he analysed the shortage of health achievements transformation.Finally,according to the actuality of health achievements transformation in hospital,the author proposed some countermeasures,such as strengthening the application of scientific achievements and the transformation consciousness,reforming the management system,reinforcing the construction of talents rank,intensifying the construction of promotion institution of scientific and technological achievements,and enhancing the hospital and enterprise' s joint,actively absorbing research funds.

The estrogen receptor (ER) coregulators regulate the transcriptional activities of ER,and then affect the transcription and translation of target genes mediated by ER.ER coregulators not only plays an important role in the initiation,progression and metastasis of breast cancer,but also involves in the response to endocrine therapy of breast cancer via the genomic and nongenomic function.