1.Determination of Berberine in Sanhuang Tablets
Yinghong ZHU ; Qingming ZHANG ;
Chinese Traditional Patent Medicine 1992;0(01):-
Objective: To establish a method for the determination of berberine in Sanhuang Tablets. Methods: By means of colorimetry the complex was determined by combining picric acid and berberine hydrochloride. Results: The method is reproducible. The average recovery was 103.3%. Conclusion: The method can be used for content determination of berberine in Sanhuang Tablets.
3.Effects of estrogen on the cultured osteoclasts in vitro
Daohai LU ; Zhongmin ZHANG ; Yinghong CHEN
Chinese Journal of Geriatrics 2000;0(06):-
Objective To study the effects of estrogen on the cultured osteoclasts in vitro. Methods The acid phosphatase (ACP) and tartrate resistant acid phosphatase (TRAP) activities were measured kinetically and the introcellular hydrogen ions were calculated with the confocal laser scanning microscopy(CLSM). The area and number of the resorption pits were determined with the Leica Quantimet 500 system. Bone resorption pits were observed with scanning electronicmicroscope. Results With the estrogen concentration increased, the ACP and TRAP activities decreased from (1 69?0 13) U/L and (1 60?0 14) U/L to (1 16?0 31) U/L and (0 93?0 34) U/L, respectively (experiment group vs control group, P
4.The chemical constituents of Rhododendron ovatum Planch
Ziming FENG ; Yinghong WANG ; Peicheng ZHANG
Acta Pharmaceutica Sinica 2005;40(2):150-152
Aim To study the chemical constituents of Rhododendron ovatum Planch. Methods The chemical constituents were isolated and purified by silica gel column chromatography and identified on the basis of their physiochemical and spectral data. Results Seven compounds were isolated and identified. Their structures were established as 3,5,7-trihydroxylchromone 3-O-β-D-xylopyranoside (I), taraxerol (II), β-sitosterol (III), betulinic acid (IV), quercetin (V), quercetin-3-O-α-L-rhamnopyranoside (VI), and D-glucose (VII). Conclusion Compound I is a new compound. Compounds II-VII were isolated from this plant for the first time.
5.Knowledge about Hand Hygiene among Outpatients:An Investigation
Ying LI ; Yinghong WU ; Lijie ZHANG
Chinese Journal of Nosocomiology 2006;0(03):-
OBJECTIVE To investigate the knowledge about hand hygiene among the outpatients and then take some valid methods to enhance their understanding about hand hygiene.METHODS By randomly asking patients from two departments in a level A of the tertiary hospital,one was a digestion and the other was a non-digestion department question designed by ourselves.The data were generally described by percentage and ?2 test with SPSS 6.0.RESULTS All the patients had not enough and correct hand hygiene knowledge from both two groups.The patients from digestion department paid more attention on their hand hygiene than from non-digestion one.The most patients from both two groups thought they needed to know more about hand hygiene and hospital should equipped with some fast hands cleaning instruments.CONCLUSIONS It is a very important duty for nurses to explain and publicize more about general hygiene and show patients how to clean their hands in order to decrease hospital infection.
7.Analysis of symptoms in patients with allergic rhinitis.
Yinghong ZHANG ; Li ZHU ; Lin WANG
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(2):133-135
Adolescent
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Adult
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Aged
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Child
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Eye
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Female
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Humans
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Male
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Middle Aged
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Nose
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Rhinitis, Allergic
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diagnosis
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Skin Tests
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Thorax
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Young Adult
8.Clinical Observation of Yinhe Gargle for Disinfection of Infected Root Canals
Liuying QI ; Zhongli CHEN ; Xing ZHANG ; Ying LIU ; Yinghong ZHANG
Journal of Guangzhou University of Traditional Chinese Medicine 2014;(5):743-745
Objective To evaluate the clinical efficacy of Yinhe gargle on infected root canals. Methods Forty-seven cases of root canal infection ( involving 48 affected teeth) were randomly divided into two groups. The experimental group (22 cases involving 22 affected teeth) was given intracanal medicament with Yinhe gargle, and the control group ( 25 cases involving 26 affected teeth) was given root canal disinfection with camphor phenol. After intracanal medicament for one week, therapeutic effect and safety were evaluated in the two groups. Results ( 1) During root canal disinfection, none of the subjects had adverse reaction such as hypersensitiveness or cauterization of soft tissue. Canal obturation was available after the first intracanal medicament for the infected root canals. (2) After intracanal medicament for one week, the effective rate in the experimental group was 95.45%, and was 100.00%in the control group, the difference being in significant (P>0.05). Conclusion Yinhe gargle has similar disinfection effect to camphor phenol on infected root canals, and it can be used as disinfection medicament for root canals.
9.Effects of Panax notoginseng saponins on mRNA expressions of interleukin-1 beta, its correlative factors and cysteinyl-aspartate specific protease after cerebral ischemia-reperfusion in rats
Yinghong TANG ; Shuping ZHANG ; Yan LIANG ; Changqing DENG
Journal of Integrative Medicine 2007;5(3):328-32
OBJECTIVE: To investigate the effects of Panax notoginseng saponins (PNS) on mRNA expressions of interleukin-1 beta (IL-1 beta), interleukin-1 receptor type I (IL-1RI), interleukin-1 receptor antagonist (IL-1ra), intercellular adhesion molecule-1 (ICAM-1), cysteinyl-aspartate specific protease-1 (caspase-1), caspase-3 and caspase-8 after cerebral ischemia-reperfusion in rats. METHODS: Focal cerebral ischemia reperfusion in rats was induced by the method of nylon monofilament via the internal carotid artery. PNS was administered intraperitoneally respectively five minutes before cerebral ischemia and twelve hours after cerebral ischemia. After cerebral ischemia for two hours followed by reperfusion for twenty two hours, the mRNA expressions of IL-1 beta, IL-1RI, IL-1ra, ICAM-1, caspase-1, caspase-3 and caspase-8 in brain tissue were determined by reverse transcription polymerase chain reaction assay. RESULTS: After cerebral ischemia for two hours followed by reperfusion for twenty two hours, the mRNA expression levels of IL-1 beta, IL-1RI, IL-1ra, ICAM-1, caspase-1, caspase-3 and caspase-8 in brain tissue in the untreated group were obviously elevated as compared to those in the sham-operation group (P<0.05 or P<0.01). The mRNA expression levels of IL-1 beta, IL-1RI, IL-1ra in brain tissue in the PNS group were lower than those in the untreated group, but higher than those in the sham-operation group, and without statistical differences as compared to those in the sham-operation group and in the untreated group (P>0.05). The mRNA expression level of caspase-3 in brain tissue in the PNS group was significantly lower than that in the untreated group (P<0.05), but PNS had no effect on the mRNA expression levels of ICAM-1, caspase-1 and caspase-8 in brain tissue. CONCLUSION: PNS can inhibit the mRNA expression of caspase-3, slightly inhibit the mRNA expressions of IL-1 beta and its correlative inflammatory factors in brain tissue. The protective effects of PNS on cerebral injury induced by ischemia-reperfusion may be related to inhibiting the mRNA expressions of caspase-3, IL-1 beta and its correlative inflammatory factors in brain tissue.
10.Changes of TXA_2 and PGI_2 content and anticoagulative and fibrinolytic activity in the plasma in a rat model of topical FeCl_3-induced carotid artery thrombosis
Yinghong TANG ; Yan LIANG ; Jing YANG ; Shuping ZHANG ; Changqing DENG
Chinese Pharmacological Bulletin 2003;0(11):-
0.05).Ticlopidine could inhibit thrombosis induced by ferric chloride,but it did not influence TXB_2 and 6-keto-PGF_1? content and activities of AT-Ⅲ and PC in the plasma.Ticlopidine could enhance activity of t-PA in the plasma.It may be related to the decrease of t-PA consumption by inhibiting thrombosis.Antithrombosis action of ticlopidine may not be concerned to inhibiting production of TXB_2 of platelet.LMWH could inhibit thrombosis also,but it did not influence TXB_2 and 6-ketoPGF_(1?) content in the plasma.LMWH could enhance activities of t-PA and t-PA/PAI-1 ratio,which may be related to the promoting release of t-PA in vascular endothelial cells.LMWH could reduce activity of AT-Ⅲ,which may be concerned with combination of LMWH and AT-Ⅲ result in AT-Ⅲ consumption.Conclusion Ferric chloride may induce occlusive thrombosis in rats.Thrombosis may be associated with activation of platelet and blood coagulation system,lower of anticoagulative protein and fibrinolytic activity.