Objective To explore the relevance between resting heart rate (RHR) and metabolic syndrome in young and middle-aged patients with essential hypertension.Methods Three hundred and forty patients with essential hypertension were enrolled in this study.According to the RHR,the hypertension patients were categorized into 3 groups:group RHR 1 (RHR ＜ 70 bpm,52 cases),group RHR 2(RHR 70-85 bpm,168 cases) and group RHR 3 (RHR ＞ 85 bpm,120 cases).The RHR,blood pressure,fasting blood glucose,triglyceride,high density lipoprotein cholesterol,2 h postprandial blood glucose and waistline were determined and compared.Results The level of fasting blood glucose,2 h postprandial blood glucose,triglyceride,waistline,systolic pressure,diastolic pressure,and high density lipoprotein cholesterol in group RHR 1 were (4.96 ± 0.65) mmol/L,(6.85 ± 0.51) mmol/L,(1.69 ± 0.43) mmol/L,(78.3 ± 6.5) cm,(143.2 ± 5.6) mmHg (1 mmHg=0.133 kPa),(85.4 ±5.2) mmHg and (1.08 ±0.64) mmol/L.In group RHR 2 they were (5.66 ± 0.77) mmol/L,(7.94 ± 0.66) mmol/L,(2.58 ± 0.75) mmol/L,(88.6 ± 7.3) cm,(156.7 ± 6.1) mmHg,(93.6 ± 4.7) mmHg,and (0.95 ± 0.59) mmol/L.In group RHR 3 they were (6.85 ± 0.73) mmol/L,(8.97 ± 0.59) mmo]/L,(3.52 ± 0.90) mmol/L,(95.8 ± 9.2) cm,(164.3 ± 7.4) mmHg,(101.2 ± 3.5) mmHg and (0.78 ± 0.33) mmol/L.With the increase of RHR,the level of fasting blood glucose,2 h postprandial blood glucose,triglyceride,waistline,systolic pressure and diastolic pressure increased step by step,high density lipoprotein cholesterol decreased step by step,and there were significant differences (P ＜ 0.05).Correlation analysis showed that RHR was positively correlated with fasting blood glucose,2 h postprandia blood glucose,triglyceride and waistline (r =0.52,0.45,0.50,0.36,P ＜ 0.05),and negatively correlated with high density lipoprotein cholesterol(r =-0.39,P ＜ 0.05).Conclusion RHR is closely related to the metabolic disorder in young and middle-aged patients with essential hypertension,and doctors should pay attention to the heart rate control while lowering blood pressure.

Pulmonary embolism(PE)is a relatively common cardiovascular emergency.Haemodynamic instability is the characteristics of massive pulmonary embolism,with a mortality as high as 20%.The goals of PE treatment are to remove the thromboembolic obstruction rapidly,exert beneficial effects on haemodynamic parameters and save the lives of patients.Haemodynamic and respiratory support is of vital importance in some critically ill patients with PE.The basic treatment is anticoagulation,the methods of which are different for PE during pregnancy,among cancer patients,with right heart thrombi and with heparin-induced thrombocytopenia.

Objective To optimize the medium for rhizomatous induction of Belamcanda chinensis in vitro. Methods By plant tissue culture technology, the effects of various carbon source, NAA, and active carbon at different concentration on the rhizomatous formation of B. chinensis in vitro were studied. Results MS+6-BA 2.0 mg/L+NAA 0.5 mg/L+6% white sugar was the optimal medium for the rhiz-omatous formation of B. chinensis in vitro. Active carbon should not be added to the medium. The germination rate of rhizomatous in vitro was 61.03% on the MS + BA 2.0 mg/L+3% white sugar. Conclusion Sugar concentration is the main factor of the influence on the rhizomatous formation of B. chinensis in vitro.

ObjectiveTo investigate and analyze the influence of modified admission education on im-provement of satisfaction degree of patients with coronary heart disease. Methods300 patients with coronary heart disease were randomly divided into the observation group and the control group with 150 cases in each group after admission. The observation group adopted diversified admission education, while the control group received simple education style, the satisfaction degree of both groups was compared. ResultsThe satisfac-tion degree of the observation group was higher than that of the control group. ConclusionsAdoption of di-versified admission education is beneficial to improve the satisfaction degree of patients, increase their cognition degree, thus make patients cooperate with treatment and nursing better and facilitate their rehabilitation.

Objective:To study the possible therapeutic mechanisms of triptergium wilfordi in treating glomerulonephritis.Methods:Thirty seven patients with IgA nephropathy were detected urine mononeuclear cells densities with flow cytometry during taking triptergium wilfordi tablets,30 healthy people were used as normal control.Results:The patients urine CD3 +?CD4 +?CD8 +?CD14 + and CD44 + cells densities were much higher than normal control's,but obviously decreased after triptergium wilfordi treatment.CD4/CD8 ratio was much lower in patients than in normal control.It increased markedly after treatment.Patients who reached remission initially had a lower CD4 + cell percentage and higher CD14 + cells percentage than those showed no response to triptergium wilfordi treatment.Conclusion:Triptergium wilfordi could modulate kidney immune cell function and adhesive molecule CD44 expression.Its effects were associated with renal immunity.

OBJECTIVE: To develop a rapid, accurate and sensitive method for the determination of nifedipine, nitrendipine and nimodipine which were added into traditional patent medicine illegally. METHODS: The LC-MS method was used to detect the extractive of Chinese patent medicine for antihypertension in respects of relative molecular mass, tandem mass spectrometry fragment, retention time, UV spectrum. The compounds added into the Chinese patent medicine were identified by comparing with standard sample in terms of spectrum, chromatographic and mass spectrometric behavior. RESULTS: According to four aspects of determination, nifedipine, nitrendipine and nimodipine were found in three kinds of Chinese patent medicine for antihypertension. CONCLUSION: The method is selective and sensitive for the detection of nifedipine, nitrendipine and nimodipine which were added into traditional patent medicine illegally.

The aim of this study was to investigate the role of adhesion molecules P selectin (CD62P) in the pathogenesis of primary nephrotic syndrome (NS) and its relationship with its different pathological types. The expression of CD62P in renal biopsy tissue and peripheral blood platelets from patients with NS were measured with direct immunofluorescence labelling monoclonal antibody and flow cytometry respectively. The results showed that CD62P was expressed in 140/165 cases with NS (84 8%). Among these patients, renal expression of CD62P was lower in MN and FSGS patients than that in MPGN and MsPGN patients, and even lower in MCD patients. The expression of CD62P in peripheral blood platelets of patients with NS was significantly higher than that in the normal control group( P

Objective To study some biological properties of mouse bone marrow stromal cells(MSCs)and their ability to differentiate endothelial cells and surviving ability in ischemic tissue,and provide an experimental foundation for applying MSCs to ischemic repair.Methods After the tibias and femurs were dissected from 5-to 6-week-old mice from Jan.2005 to Nov.2005,the marrow was flushed out with ice-cold DMEM/F12 medium.The mononuclear cells of the marrow were obtained with density gradient centrifugation and the plated and cultured in DMEM/F12 medium.The cultured cells in vitro were induced with endothelial cell growth supplement.The ability of MSCs was also was examined in ischemic tissue.Results The adherent fibroblast-shaped cells approached confluence in single layer 12~16 d after plating.The cultured MSCs in vitro differentiated into endothelium.Numerous scattered DAPI-labeled cells were found in the specimen seven days after implantation,and hematoxylin and eosin staining of the adjacent section showed concordance between dense hematoxylin staining and presence of DAPI epifluorescence,and there was no obvious inflammatory response.Conclusion The subcultured MSCs possess potential to differentiate into endothelial cells.MSCs show stable growth in vitro,easy survival in the subculture and rapid proliferation in present culture condition.MSCs may be used in therapy for myocardium ischemia.

Objective To approach the related factors and emergency endoscopic hemostasis measures for duode-nal papilla hemorrhage after ERCP, then sum up the clinical experience of prevention and treatment. Methods Clin-ical data of 27 patients with duodenal papilla hemorrhage treated by emergency endoscopic hemostasis were retro-spectively analyzed. Results 26 cases treated by emergency endoscopic hemostasis were successful, the success rate was 96.30 %. Conclusions Prevention is very important for duodenal papilla hemorrhage. Once bleeding after ER-CP, emergency endoscopic hemostasis is the most direct, simple and effective measure.

BACKGROUND:Studies have shown that human umbilical cord mesenchymal stem cel s can improve pulmonary ventilation function by reducing inflammations. OBJECTIVE:To observe the therapeutic effect of human umbilical cord mesenchymal stem cel transplantation on acute lung injury. METHODS:Thirty Sprague-Dawley rats were randomized into normal group, model group and experimental group. Rats in the latter two groups were used to establish animal models of acute lung injury by intratracheal instil ation of lipopolysaccharide. One hour after modeling, rats in the experimental group were intratracheal y administered human umbilical cord mesenchymal stem cel suspension (0.1 mL, 1×106 cel s), and those in the other two groups were given normal saline in the same dose intratracheal y. Twenty-four hours after treatment, the pathological changes of lung tissue were observed using hematoxylin-eosin staining;the wet and dry weight ratio of the lung tissue and the levels of serum interleukin-1 and interleukin-8 were detected. RESULTS AND CONCLUSION:Compared with the normal group, the wet and dry weight ratio of the lung tissue and the levels of serum interleukin-1 and interleukin-8 were significantly increased in the model group (P<0.05), while compared with the model group, these levels were significantly decreased in the experimental group (P<0.05). Hematoxylin-eosin staining results showed clear alveolar space structure with complete alveolar septum in the normal group. In the model group, the alveolar septum was markedly thickened, and there was visible pulmonary capil ary hyperemia, edema, as wel as a large amount of inflammatory cel infiltrations in the pulmonary capil aries and alveolar space. Edema fluid rich in proteins was observed in a part of the pulmonary alveoli, and an extensive transparent membrane formed in the alveolar space. In the experimental group, the alveolar structure was clear, but the alveolar septum became thickened, and red blood cel s and a smal amount of infiltrated inflammatory cel s were leaked from the pulmonary interstitial tissue. In conclusion, human umbilical cord mesenchymal stem cel transplantation for treatment of acute lung injury can reduce inflammatory factor levels and al eviate lung injury.