Objective To research the association between the prognosis of Acinetobacter baumannii infection and drug resistance among patients in the intensive care unit( ICU). Methods Eighty-four patients with Acine tobacter baumannii infection from February 2003 to April 2009 in Peking University Shenzhen Hospital ICU were retrospectively analyzed and divided into alive group(59 patients) and dead group(25 patients) by using 30-day mortality. The impact of drug resistant Acinetobacter baumannii on mortality was assessed by binary logistic regression. Results The mortality of patients with Acinetobacter baumannii infection was related to age, haematological malignancy, the Chanson Comorbidity Index, APACHEII score, multidrug-resistant Acinetobacter baumannii ( MDRAB) , carbapenem-resistant Acinetobacter baumannii (CRAB ) , septic shock and antimicrobial inappropriate therapy by univariate analysis( P < 0.05). Binary logistic regression revealed that CRAB( OR = 3. 360,95%CI: 1. 563 ～7.223,P< 0.05)and the Charison Comorbidity Index( OR = 1. 633,95% CI: 1. 201 ～2. 221 ,P <0.05) were independent risk factors. Conclusions The prognosis of Acinetobacter baumannii infection was closely correlated with drug resistance. The patients with CRAB infection had a higher mortality.

[Objective] To investigate the improvement of abdominal symptoms,quality of life,and depression of Soothing Liver Regulating Qi and Regulating Spirit and Relieving Depression massage combined with western medicine treating diarrhea type irritable bowel syndrome(IBS) patients.[Methods] From January 2015 to December 2016,a total of 60 diarrhea type IBS patients were randomly divided into control group and treatment group.The control group was treated with Trimebutine Maleate Dispersible Tablets,while the treatment group was treated with Soothing Liver Regulating Qi and Regulating Spirit and Relieving Depression massage on the base of the control group.Two groups patients were treated for 4 weeks.The abdominal symptoms,quality of life,depression and serum 5-HT were collected before and after treatment.[Result] After 4 weeks treatment,the abdominal pain/abdominal discomfort,diarrhea,HAMD of both groups were lower than before treatment,and the treatment group was lower than the control group(P＜0.05).The SF-36 of both groups were higher than before treatment,and the treatment group was higher than the control group(P＜0.05).Serum 5-HT of both groups were lower than before treatment,and the treatment group was lower than the control group(P＜0.05).[Conclusion] Soothing Liver Regulating Qi and Regulating Spirit and Relieving Depression massage combined with western medicine can effectively improve the abdominal symptoms and quality of life for diarrhea type IBS patients.

Objective To assess antibiotic prescription habits,cost pattern and the prospective intervention in Intensive Care Unit was analyzed.Methods Data on antibiotic utilization and antibiotics susceptibility were col-lected prospectively from individual electronic charts from July 2014 to September 2014.Results 225 of 246 patients surveyed used antimicrobial during the ICU stay,and antibacterial drug utilization rate was 91.46%.Cefperazone-sulbactam and piperacillin-tazobactam were the most prescribed medications.Total defined daily dose ( DDDs) was 1121.1 DDDs.Bacteria culture was 98 positive in 677 cases and 101 pathogenic bacteria were isolated.Conclusion Interventional programs should focus on promoting infectious control with rational antibiotic prescription aimed at mini-mizing the future emergence of bacterial resistance and futile.

Objective To investigate the perioperative management and video-assisted thoraco-scopic (VATS) extended thymectomy for myasthenia gravis ( MG ). Methods A total of 102 patients who received extended thymectomy for MG from June 2000 to August 2007 were divided into 2 groups by operative approach: 51 in the VATS group and 51 in the full median sternotomy group. The clinical features, including operation time, operation blood loss, post-operative drainage, inci-dence of crisis, hospital stay, and the 1-year total effective rate after the operation were compared. Complications were treated exactly and cholinesterase inhibitors were applied to control the symptom of MG before the operation. The whole thoracic gland and fat in the anterior mediastinum were removed. Steroid, cholinesterase inhibitors and plasmapheresis were used to prevent and cure masthemic crisis after the operation. Results No patients died in the operation and hospitalization. There was no sig-nificant difference in the operation time [(128.14±34.82) min vs. (130.46±28.71) min] and the 1-year total effective rate after the operation (85.8% vs. 87.2% )in the 2 groups(P>0.05).The operation blood loss, postoperative drainage, incidence of crisis, and postoperative hospital stay in the median sternotomy group were higher or longer than those in the VATS group [(93.77±21.64) mL vs. (45.42±10.96)mL,P<0.05; (174.65±21.64)mL vs. (101.33±28.76) mL,P <0.01;7.84% vs. 1.96%, P<0.05;(14.23±3.17)d vs. (8.37±1.18)d, P<0.05)]. Conclusion VATS extended thymectomy for myasthenia gravis is safe, less invasive and effective. Preoperative preparation and the perioperative managemant can decrease the incidence of crisis. The plasmapheresis is the key of curing masthemic crisis.

Objective:To exploring the developmental characteristics of 8 to 16 year-old children of parental authority and behavioral autonomy.Methods:251 children and adolescents aged 8 to 16 were investigated by questionnaires and interviews in light of different domains(moral,conventional,personal,and friendship)to research about parental authority and behavioral autonomy.Results:①The results suggested that children and adolescents' justment of legitimacy was significant in different domains;②From 8 to 16,age had significant differences in all issues;③From 8 to 16,children and adolescents' justification of personal issues had significant difference among grades,and 55% of them focused on personal reasons.④From 8 to 16,there were significant differences in justment of legitimacy in personal diary between boys and girls.Conclusion:From 8 to 16,children and adolescents' justment of legitimacy and their obedience to parental authority are significant in different domains.With the increasing of years,the development of their behavioral autonomy is rising.

Objective To establish a simple,rapid,accurate and practical method for determination of chloride in drinking water by chloride ion-selective electrode.Methods The chlorine ion-selective electrode was used as indicator electrode and type217saturated calomel electrode of the double salt bridge(the lower salt bridge was filled with a solution of0.1mol/L potassium nitrate)was used as reference electrode.The contents of chloride in water were calculated using the calibration curve method in the electrolyte containing0.1mol/L potassium nitrate.Results The linear range of the method was10 -5 -10 0 mol/L and the best lin ear range was10 -4 -10 -1 mol/L with r=0.9999and the slope=53.5?0.667.The detection limit,recovery rate and coefficient of variation(CV)were0.3545mg /L,94.2%-102.2%(mean98.1%)and2.8%respectively.The suitable pH for the determination of the method was2-10.In the solution containing3.545mg/L chloride,50times more BrO -3 ,NO -2 ,CH 3 COO - ,SO 42- ,F - ,CrO 42- and10times more CO 32- could not interfere with the detection,but Br - and I - were interfering factors.The re-sults obtained by the chlorine ion-selective electrode method and the national standard determination method showed no statistic differences.Conclusion The method was easy to operate,rapid,accurate and suitable for determination of chloride in water.

0.05). Conclusions The detections of survivin and MCM5 in urine sediments are sensitive and non-invasive diagnostic tests for bladder cancer.The combination of survivin and MCM5 may be more effective than either of them alone.

Objective To investigate the protective effects and mechanism of nitric oxide(NO) inhalation on rabbit lung ischemia/reperfusion injury. Methods Total 32 Japanese white rabbits were randomly divided into four groups, each group containing 8 rabbits. In group A, animals received only thoracotomy. In group B, after animals left chest were opened, their left main pulmonary arteries were occluded for 2h, then reperfused for another 2h. In group C, animal inhaled 20 ppm NO for 30min since the eleventh minute after reperfusion. In group D, animal inhaled 60ppm NO for 30min since the eleventh minutes after reperfusion. The levels of blood PaO 2, cGMP, slCAM-1, XOD and MDA were measured before operation and after reperfusion. The pathologic changes of left lung was observed. The wet/dry (W/D) weight ratio of left lung tissue was measured. Results Both PaO 2 and cGMP levels after reperfusion in group B were much lower than that in groups A, C and D(P

Objective To evaluate the influence of glutamine(Gln)-enriched total parenteral nutrition on nutritional status,suppression of excessive systematic inflammatory responses and immunological competence on critical patients in ICU.Methods Sixty critical patients were included in a randomized controlled clinical trial and divided into Gln group and control group,with 30 case for each group.Both groups received isocaloric and isonitrogenous nutritional formulas on everyday.In addition,Gln was added to Gln group from day 1 to day 8.On day 1 and 8 after treatment,serum albumin,proalbumin,transferin,IgA,IgG and IgM were determined respectively.The plasma level of CPR,IL-6,TNF-?,blood suger,liver function were also monitored.Results (1)The level of serum albumin,proalbumin,transferin,IgA,IgG,and IgM decreased in all patients.Albumin,proalbumin,transferin,IgA,IgG,and IgM were significantly improved in Gln group on day 8 compared with control group and before treatment(P

Objective To investigate the effect of dihydromyricetin on proliferation and apoptosis of breast cancer MCF-7 cells.Methods From March 2014 to February 2015,breast cancer MCF-7 cells were treated with 99% pure DMY as an inhibitor.MTT assay,flow cytometry and immunocytochemistry were used to analyze the proliferation,apoptosis and protein expression of breast cancer cell MCF-7.Results When the DMY concentration was higher than 20 μg/mL,the inhibitory effect appeared,but not good.When 40 and 80 μg/mL DMY were used,the proliferation of MCF-6 cells were significantly inhibited,and have different degrees of sensitivity to it.When DMY was 80 μg/mL,the IC50 was 226.9 μg/mL.The inhibition rate and IC50 were compared with 0 μg/mL DMY,there was significant difference(P<0.05).Indicating that the relatively high concentration of DMY could significantly improve the patient′s condition of breast cancer;MCF-6 cells treated with 40 and 80 μg/mL DMY could induce G2/M phase arrest in DMY with concentration greater than 20 μg/mL(P<0.01),and showed significant cell apoptosis death phenomenon.At the same time,G0/G1 phase was also blocked and S phase cells decreased significantly,the difference was significant(P<0.05).The positive rate of PCNA in breast cancer MCF-6 cells significantly decreased(P<0.01) when the DMY concentration was higher than 50 μg/mL,the positive rate was> 50%,especially in DMY with 80 μg/mL,the positive rate was 10.00%.Compared with 0 μg/mL DMY,the difference was significant(P<0.05).Conclusion The use of dihydromyricetin in breast cancer patients can effectively inhibit the rapid increase of cancer cells,accelerate apoptosis,slow down the patient′s condition,the effect is outstanding.