Objective:To screen auramine O in Guci tablets. Methods:Auramine O was detected by HPLC method on a Diamon-sil-C18(250mm×4.6mm,5μm)columnwiththemobilephaseofacetonitrile-0.025mol·L-1monopotassiumphosphatebuffersolu-tion(containing 0. 2% triethylamine,adjusting pH to 3. 0 with phosphoric acid)(36∶65)at the flow rate of 0. 8 ml·min-1. The detec-tion wavelength was 432 nm and the column temperature was 35℃. Results:The LOD of auramine O was 0. 05ng. Among 45 batches of tested samples, auramine O was found in seven ones. Conclusion:The method is simple, rapid and accurate, which can guarantee the drug safety of Guci tablets.

Objective To investigate the therapeutic effect of distal radius fractures operation.Methods Eighty distal radius fractures patients who were treated from January 2010 to January 2011 were divided into observation group and control group by random digits table method,each group had 40 cases.The observation group was treated with plate internal fixation,the control group took closed reduction and splint external fixation.The palmar tilt,radial ulnar deviation,axial shortening before treatment and after treatment for 3 months were compared between two groups.The clinical curative effect after treatment for 1 year was compared.Results The palmar tilt,radial ulnar deviation,axial shortening before treatment between two groups had no significant difference (P ＞ 0.05).After treatment for 3 months,the palmar tilt,radial ulnar deviation,axial shortening in observation group were (17.0 ± 1.6)°,(23.0 ± 2.1)°,(1.0 ± 0.2) mm,in control group were (4.0 ± 0.8)°,(10.0 ± 1.1)°,(4.0 ± 1.1) mm,there was significant difference(P＜ 0.05).After treatment for 1 year,the excellent and good rate in observation group was 90.0％(36/40),significantly higher than that in control group [50.0％ (20/40)] (P ＜ 0.05).Conclusion Plate internal fixation in the treatment of distal radius fractures is more conductive to the wrist recovery of joint structure and function,reduce the occurrence of wrist joint dysfunction,improve the quality of life,and is worth to be applied in clinic.

Equipment Design ; Humans ; Pacemaker, Artificial

Objective To evaluate the safety of epidural resiting and the incidence of postdural puncture headache following accidental dural puncture. HZ Methods Among 63 patients of ASA I-II undergoing lower abdominal or lower extremity surgery, forty-two with postdural puncture who had received epidural resiting were randomly assigned to patient controlled epidural analgesia (PCEA) ( n =21) or control treatment ( n =21). The remaining 21 patients who had successful epidural insertion received the routine PCEA. All patients after operation received 48-hours monitoring of BP, HR, RR and SpO_2. The analgesic effect of each treatment and the occurrence of headache were also assessed. Results All operations were uneventful under epidural anesthesia. A satisfactory level of analgesia was observed in patients who received PCEA. There was no statistically significant difference in pain control between patients who had had epidural resiting and who received PCEA and those who received routine PCEA. No respiratory inhibition was observed. The occurrence of headache in the epidural resiting group was 19% (4/21), compared to 57% (12/21) in the control group ( P

Objective:To explore the diagnostic value of multi-tumor marker protein biochip detective system for primary hepatic cancer.Methods:Using pathological examination,or at least one-year clinical follow-up results for the gold standard,we divided patients with liver disease into three two groups:primary liver cancer group,benign liver disease group and normal group,using protein chip technology to detect the measurement serum tumor markers.Results:CA199,CEA,CA242,FER,AFP,CAl25 and CA153 were significantly higher in PHC group than those in benign liver disease group and normal group(P

Objective: To establish a method for the determination of the related substances in pantoprazole sodium capsules. Methods:A Kromasil C18 column (250 mm × 4. 6 mm, 5 μm) was used. 0. 01 mol·L-1 monopotassium phosphate solution (adjus-ting pH to 7. 0 with phosphoric acid)-acetonitrile was adopted as the mobile phase with gradient elution. The detection wavelength was 289 nm and the column temperature was 40℃. The injection volume was 20μl and the flow rate was 1. 0 ml·min-1 . Results:Panto-prazole sodium and its degradation substances could be well separated. The limit of detection and quantification of pantoprazole sodium was 0. 16 ng and 0. 48 ng, respectively. Conclusion:The method is simple, specific and sensitive, and can be applied to determine the related substances in pantoprazole sodium.

Objective To analyze the falling events using root cause analysis (RCA) in hospitalized elderly patients in order to supply theoretic reference for precaution of falling events.Methods RCA method was used to analyze the root cause against 62 cases of nursing adverse events of hospitalized elderly patients' falling.Results Hospitalized elderly patients' falling is related to various factors such as body,disease,medicine,psychology,environment and so on.The root cause lied in the elderly' physical function decline,risk factors of external environment,unfulfilled health education,and lack of the care and support system for the elderly,among which cerebral infarction belonging to the disease factors accounted for 22.58％ (14/62),which was the highest;next was senile dementia [20.97％ (13/62)].The rates of agents against psychiatric disorders belonging to the medicine factors and no braking of bed pulley and no use of blister light belonging to environmental factors were the lowest,all were 3.23％ (2/62).Conclusions The falling reason of elderly inpatients can be found out by RCA in order to attain the purpose of improving procedure and prevention in advance.

Anti-retroviral (ARV) drugs have been successfully used for HIV/AIDS therapy,and their uses in pre-exposure prophylaxis (PrEP) for HIV infection is becoming increasingly important,and is drawing more attentions.Reports on seven large scale,multicentric clinical trials provide significant clinical data for further study and implementation of PrEP.This article reviews the current situation,necessity,questions,problems and prospects of PrEP for HIV infection.

Toll-like receptor( TLR) ,which belongs to pattern recognition receptor,is type I transmem-brane glycoprotein. TLR activates natural immune and induces several cytokines. Foreign studies have showed that bacterial infection is closely related with TLR. In this paper,the relationship between TLR and bacterial in-fection is reviewd.

Atherosclerosis ; etiology ; therapy ; Humans ; Lupus Erythematosus, Systemic ; pathology ; therapy