The main acupoints Huantiao (GB 30) and Juliao (GB 29), combined with the adjunct acupoints according to the pain characteristic, were punctured, after arrival of qi, needle warming therapy was performed, and then Tuina therapy was applied after the removal of needles. Among 72 cases of sciatica treated by these therapies, 53 cases were cured, 13 cases were remarkably improved, 4 cases were effective, and 2 cases got no effect, with the total effective rate being 97.2%.

Objective To analyse effect of Danhong injection on serum levels of bone markers and healing time in postoperative patients with tibial fractures.Methods 52 patients who were diagnosed with tibial fractures in our hospital were collected and randomly divided into control group and experimental group.All patients were treated with intramedullary nailing.After the operation, on the basis of routine treatment, patients in control group were treated with ossotide injection 50 mg, dissolved in 250 mL 0.9% sodium chloride solution, one times per day.Patients in experimental group was treated with Danhong injection 20 mL, dissolved in 250 mL 0.9% sodium chloride solution, one times per day.14 days for one period of treatment, two groups were treated for two cycles.After the treatment,the serum levels of bone Gla-protein (BGP), carboxyterminal propeptide of typeⅠprocollagen (PICP), bone alkaline phosphatase (BALP) and the racture healing time were detected in all patients.Results Compared with control group post-treatment, the serum BGP and PICP levels were higher in experimental group (P<0.05);the serum BALP level was higher in experimental group (P<0.05); the average fracture healing time of the patients in experimental group was shorter (P<0.05).Conclusion Danhong injection can significantly increase the serum BGP, PICP and BALP level in postoperative patients with tibial fractures, shorten fracture healing time.

Seventy-three cases of cervical spondylosis were treated with cervico-occipital traction belt. During the conduction of traction, acupoimts Bailao (Ex-HN), Fengchi (GB 20),Tianzhu (BL 10), Jianjing (GB 21), Tianzong (SI 11) and Shousanli (LI 10) were needled. When needling sensation arrived, moxibustion was at the same time given by igniting moxa around the needles. After two-course treatment, 60 cases got clinical cure, 8 cases got marked effectiveness, 2 cases got effectiveness and 3 cases failed, the total effective rate being 95.9%.

Objective To investigate the difference and significance of the clinical index for the aged suffered from type 2 diabetes mellitus in southern part of Guizhou. Methods A total of 240 old patients of type 2 diabetes mellitus were divided into 5 groups according to the complications (non-complications, 43; cerebrovascular disease, 20; macroangiopathy, 43; peripheral neuropathy, 67; diabetic retinopathy, 67) and 5 groups according to the different nationalities (Han 90, Miao 60, Buyi 70, Shui 12, Other 8). The chemical index including serum FBG, HbAlc, TC, TG, and HDL-c of all patients were detected. The results were treated with t-test and X2 test. Results The serum levels of FBG, HbAlc, TC, TG in the diabetic patients with complications were higher than that of non-complication patients (P

Objective Preliminary study of the cough reflex sensitivity test in chronic cough patients with different gender,disease duration and causes to evaluate the clinical diagnostic significance of the test and further provide some information for the treatment of chronic cough.Methods Totally 108 chronic cough patients in our hospital were enrolled in the study with the final diagnosis of upper airway cough syndrome(UACS),cough variant asthma(CVA)and gastroesophageal reflux cough(GERC).They all went through the cough reflex sensitivity test and the retrospective analysis of the results was made.Results In 108 patients with chronic cough(76 UACS,19 CVA and 13 GERC),lgC5 was significantly slower in the female(1.80)than the male(2.40,P<0.05)and in the patients with cough duration over 12 months(1.80)than those with cough duration under 12 months(2.40,P<0.05).Cough sensitivity in patients with different causes was significantly different(P<0.05)with lower lgC5 in GERC patients(1.49)than CVA (2.40,P<0.05)and UACS(2.40,P<0.05)patients.Conclusion Cough sensitivity is different in chronic cough patients with different gender,disease duration and causes.Cough reflex sensitivity test is of great value in the etiology diagnosis of chronic cough.

BACKGROUND:Most bone marrow mesenchymal stem cel s are infused intravenously and have very low efficiency of homing to the bone marrow. However, cel infusion via the femoral approach is little reported. OBJECTIVE:To explore the distribution of luciferase gene modified red fluorescent protein transgenic bone marrow mesenchymal stem cel s in vivo through different infusion routes. METHODS:Luciferase gene modified bone marrow mesenchymal stem cel s at different gradients (5×106, 1×106, 1×105, 1×104) were seeded or injected into the in vitro pore plate or free femurs to observe the fluorescence imaging and select the best concentration of cel s. Luciferase gene modified bone marrow mesenchymal stem cel s at the best cel concentration were injected into the mice via the femur and the tail vein, respectively. The distribution of fluorescence and cel number in the mice were explored by using bioluminescence, pathological examination, flow cytometry and quantitative PCR. RESULTS AND CONCLUSION:Ex vivo fluorescence intensity of luciferase gene modified bone marrow mesenchymal stem cel s was positively correlated with the cel concentration;fluorescent cel s in vivo appeared in the femur first and then quickly spread to the lungs in the femur group, while fluorescent cel s in the tail vein group spread to the lungs quickly after cel infusion. Fluorescent cel s could be seen in the spleen, liver and other organs 24 hours later in the two groups. The distribution and migration of cel s in mice could be observed successful y by bioluminescence;5 minutes after cel infusion, the lungs of mice in the two groups began to emit fluorescence that could spread to the liver, spleen and other tissues 24 hours later, and the fluorescence intensity reached its peak after 15 minutes. The distribution of bone marrow mesenchymal stem cel s in mice had no significant difference between the femur group and the tail vein group. To conclude, cel injection through the bone marrow cavity and tail vein fails to promote the homing of bone marrow mesenchymal stem cel s to the bone marrow.

Objective To evaluate clinical therapeutic efficacy and adverse effecacy of transcatheter arterial chemoembolization(TACE)combined with radiofrequency capacitive heating(RCH) for metastatic hepatic carcinoma(MHC). Methods Thirty-nine cases of MHC were enrolled in this study and divided into two groups: study group(n = 19)and control group(n = 20). Before therapy, the Karnofsky's score of the patients was all beyond 60. Results The carcinoma growth rate of the study group was -(0.38 ? 0.22), while that of the control group was -(0.13 ? 0.25), showing significant statistical difference(P 0.05). Conclusion The therapeutic effect of MHC can be further improved by the treatment of TACE combined with radiofrequency capacitive heating without increase of adverse side effects.

Objective To find direct effect of Chinese herbs Bu Gu Zhi (Psoralea corylifolia L) and Bai Zhi (Dahurian angelica root) Extracts on melanocyte adhesion and migration in vitro. Methods Ethanol extracts obtained from two kind of Chinese medicinable herbs were tested. Human melanocytes were obtained from neonatal foreskins and 48-well culture dish covered with fibronectin were used to perform melanocyte adhesion assay; Motility was assessed using the micropore filter method. Results: The extracts of Bu Gu Zhi(Psoralea corylifolia L), Bai Zhi(Dahurian angelica root) obviously showed an effect in increasing of human melanocyte adhesion and migration on fibronectin. Conclusion It is suggested that Buguzhi(Fructus Psoraleae) and Baizhi(Radix Angelicae Dahuricae) might induce melanocyte adhesion and/or migration in the treatment of vitiligo.

Background Diabetic retinopathy (DR) has become the main cause of blindness in the world,But the etiology of DR is still not clear,and the results of the studies on the risk factors of DR are not completely consistent.Fully understanding the risk factors of DR has an important clinical value for the prevention and treatment of DR.Objective This study was to analyze the prevalence rate and risk factors of DR inpatients with type 2 diabetes and provide a basis for the establishment of ophthalmic intervention programs and measures for diabetic inpatients.Methods Cross sectional study was performed.Four hundred and seventy three patients with type 2 diabetes in the department of endocrinology,Dongguan People's Hospital from July 2011 to July 2012 were included.The patients were divided into DR group and non diabetic retinopathy (NDR) group.The DR group was subdivided into mild,moderate and severe non-proliferative DR (NPDR) group and proliferative DR (PDR) group.The sex and age of patients,course of diabetes,body mass index (BMI),systolic blood pressure,diastolic blood pressure,fasting blood glucose (FBG),2 hours postprandial blood glucose (2 h PBG),glycosylated hemoglobin (HbA1 c),fasting insulin,2 hours postprandial insulin,high density lipoprotein cholesterol,low density lipoprotein cholesterol,apolipoprotein A1 (APOA1),APOB,alpha lipoprotein,total cholesterol,three glycerol,urea nitrogen,creatinine,uric acid,percentage of neutrophil,24 hours urinary albumin total (ALBU-24 h) and ALBU were detected.Logistic regression was used to analyze the relationship between DR and various factors,and the risk factors of DR were screened out.Results The prevalence of DR inpatients with type 2 diabetes was 28.33％,the prevalence of mild,moderate and severe NPDR were 2.54％,16.28％ and 4.23％,respectively,the prevalence of PDR was 5.29％.The prevalence of DME was 10.36％ in the DR patients.The course of diabetes,the levels of serum lipoprotein,creatinine,ALBU-24 h and ALBU were statistically significant between DR group and NDR group (all at P＜0.05).By stepwise Logistic regression analysis,the course of disease,FBG were identified as the independent risk factors of DR (course of disease:odds ratio [OR] =1.155,95 ％ confidence interval [CI]:1.067-1.251;FBG:OR =1.313,95％ CI:1.071-1.610).Conclusions The course of diabetes,lipoprotein,creatinine,ALBU-24h,ALBU are closely related to the occurrence and development of DR.The course of diabetes and FBG are the independent risk factors of DR.

Objective To observe the clinical efficacy and adverse reactions between pemetrexed plus cisplatin and paclitaxel plus cisplatin in the treatment of advanced non-small cell lung cancer (NSCLC).Methods Forty-two cases with non-small cell lung cancer in our hospital from June 2013 to June 2015 were selected.Patients were randomly divided into observation group and control group with 21 cases in each group.The observation group was treated with pemetrexed plus cisplatin and the control group was treated with paclitaxel plus cisplatin.The clinical efficacy and adverse reactions were observed after two courses (21 days of a course) and the statistical analysis was performed.Results In the observation group and control group,response rate and clinical benefit rate were33.33％ (7/21),28.57％ (6/21) and76.19％ (16/21),71.43％ (15/21) respectively.No statistical significance were seen between the two groups (P＞0.05).The rate of hair loss (23.81％) (5/21) and the total number of adverse reactions (18.37％) (27/147) in the observation group were significantly lower than the rate of hair loss (47.62％) (10/21) and the total number of adverse reactions (25.17％) (37/147) in the control group (P＜0.05) No statistical significance was found in the incidence rate of leukopenia,neutropenia,erythropenia,thrombocytopenia,nausea and vomiting and phlebitis between the two groups (P ＞0.05) Conclusion Pemetrexed plus cisplatin is as effective as paclitaxel plus cisplatin in the treatment of advanced NSCLC with less adverse reactions and is much safer than paclitaxel plus cisplatin.Pemetrexed plus cisplatin is more valuable in the clinical treatment of advanced NSCLC.