PEGylating is an effective way for prolonging the half-time period and decreasing the immunogenicity of protein drugs. With experiments of single factor, it was proved that the optimal processes for PEGylating the fused protein of LL-37 and interferon (IFN)-α2a were: PEG molecular weight was 5,000, fused protein concentration was 0.6 mg/mL, the mole ratio of protein to mPEG₅₀₀₀-SS was 1:10, the reaction temperature was 4 °C, and the pH was 9.0, respectively. With orthogonal experiments, we proved that the influential order of 3 main factors is: the fused protein concentration > the mole ratio of protein and mPEG₅₀₀₀-SS > pH and the optimal conditions were the fused protein concentration as 0.6 mg/mL, the mole ratio of protein and mPEG₅₀₀₀-SS as 1:10, pH as 8.8. Under these optimal conditions, the average rate of PEGylated protein with 3 times parallel experiments was 86.98%. After PEGylated, the interferon activity and antimicrobial activity of fused protein could be remained higher than 58% and 97%, respectively.
Anti-Infective Agents ; chemistry ; Antimicrobial Cationic Peptides ; chemistry ; Interferon-alpha ; chemistry ; Polyethylene Glycols ; chemistry ; Recombinant Proteins ; chemistry

The high mutation rate during hepatitis B virus (HBV)replication leads to HBV quasispecies.The study of HBV quasispecies has an important significance for the hepatitis B pathogenesis,prognosis,and outcome prediction.Recently,the next-generation sequen-cing (NGS)is extensively used in many biological and medical fields due to its high throughput,ultra-deep coverage,and high sensitivity, which also brings new strategies to HBV quasispecies studies.This article describes the working principles and features of several commonly used NGS technologies,reviews the application of NGS technologies in HBV quasispecies detection in recent years,and particularly discus-ses the variations in different regions of HBV genome and the population characteristics of HBV quasispecies.For now,NGS technologies used in HBV variation detection mainly presents the advantages of high sensitivity and high throughput.However,how to lower the cost,in-crease the accuracy of sequencing,and standardize the procedure of massive sequencing data management and personalized analysis are still challenging problems.The issue about NGS that matters at present and urgently needs to be solved is how to overcome its limitations,and then put it into HBV-related studies and ultimately clinical application.

OBJECTIVE:To study the measures to improve the patients’compliance.METHODS:An investigation was carried out in136patients in a given hospital in Wuhan in the way of questionnaire and interview.RESULTS:There was no significant difference in compliance among the patients with different sex,age,education background and income,but there we_ re significant differences among the patients with different initiatives,conceptions,habits,and knowledge of drug use.CONCL_ USION:It is suggested that IMB model should be adopted to improve patients’compliance in the respect of external informa-tion,internal motivation,and behavioral skills.

The present therapy for primary hepatocellular carcinoma (HCC)consists of surgery as well as local radiotherapy and chemother-apy.However,the majority of patients are susceptible to recurrence after comprehensive treatment,and the overall treatment outcome is not ideal due to the lack of effective drugs and strategies.Increasing evidence has demonstrated that the immune system is closely related to the development,progression,metastasis,and recurrence of HCC.Thus,immune therapy,especially cellular immunotherapy,could regulate immune function and induce specific antitumor immunity to achieve the goal of controlling HCC and reducing its recurrence and metastasis, which has become an essential part in the comprehensive treatment of HCC.The findings in preclinical and clinical studies on cellular immu-notherapy for HCC data are reviewed,and the current problems are discussed.

claudin protein is the major component of tight junctions complex between cells,which plays an important role in cells junction and adhesion. Its specific expression in different tissues directly affects the biological behavior of tumor cells. In pancreatic tumors, the claudin's expression provides an important refer-ence for the diagnosis and treatment of tumors. In addition, claudin are involved in the proliferation and ap-optosis of tumor cells. In the treatment of tumors, claudin-mediated targeting therapy has great prospects forthe same.

Objective To assess the clinical efficacy of direct Stenting (DS) and conventional post-dilation stenting (CS) for A and B1 lesions in Patients with coronary artery disease.Methods Demographic characters, final baloon dilation pressure, instent angiographic results, and long term outcomes were compared between patients receiving DS (n=62) and patients receiving CS(n=51). Results The operation time in DS group was much shorter than that in CS group ( 17.2 ?8.6 min vs 26.3 ?7.1 min, P

Objective To study the effect of tail-suspending on myostatin(MSTN)expression in the soleus muscle of rats.Methods The MSTN mRNA levels in soleus muscle of tail-suspended and normal control rats were measured and compared.Results The MSTN mRNA in soleus muscle of tail-suspended rats was 2.2 times(hind limb unloading for 14 days)and 3.5 times(hind limb unloading for 30 days) higher than that in normal control rats (P<0.05).The relative wet weight of the soleus muscle in tail-suspended rats was decreased by 11%(unloading 14 days)and 1 9%(unloading 30 days),respectively,compared with that in normal control rats.Conclusion The expression of MSTN mRNA is elevated in the soleus muscle of tail-suspended rats,probably due to atrophy of the soleus muscle.

Objective To develop the public mood scale and test its reliability and validity,and explore the components construct of the public mood.Methods 423 college students responded to the inventory.Correlation analysis,exploratory factor analysis and confirmatory factor analysis were applied to the data analysis.Results Public mood scale was consisted of two dimensions,positive emotions and negative emotions.Factor analysis indicated that the two factors explain 61.3％ of the variance with factor loading ranging from 0.68 to 0.86;The Cronbach' s coefficients of positive and negative public mood were 0.81 and 0.76 respectively; Conffirmatory factor analysis indicated that the public mood scale had ideal structure validity.Joint factor analysis showed that public mood and personal mood were separated.Positive public mood had positive correlations with Big Five personalities,which were extraversion,agreeableness,responsibility and openness (r =0.22-0.33,P＜ 0.01),negative correlation was found between neuroticism and positive public mood(r=-0.25,P＜0.01).Positive public mood had significant positive correlation with government satisfaction,economic development confidence and social stability(r=0.34-0.45,P＜0.01),while they had significant negative correlations with negative public mood (r =-0.32--0.24,P＜ 0.01).Conclusion The public mood scale obtained in the present study has ideal validities and reliabilities and it is suitable to assess the public mood.

Objective To explore effect of immune function of children with EB virus infectious mononu-cleosis (IM). Methods From Sep. 2011 to Jun. 2013, a total of 62 cases of children with IM were analyzed retrospectively. The proportion of T-lymphocyte subsets ,B-lymphocytesand and NK cells in EBV-DNA group with positive and negative were compared. Results The numbers of IM EBV-DNA-negative cases were 18 (29.03%), the numbers of IM EBV-DNA-positive cases were 44(70.97%), the averages of EBV-DNA in positive group is 6.1 × 103/mL. CD3+,CD8+cell ratios in IM children are significantly higher than control group (P<0.01), CD4+, CD19+cell ratios are significantly lower than control group (P<0.01), NK cells are not significantly different (P>0.05). In the IM children, CD4+, CD19+cells in DNA-positive group are significantly less than DNA-negative group (P<0.01), CD8+cells are higher than DNA-negative group (P<0.05), NK cells are not significantly different (P>0.05). Conclusions Positive blood EBV-DNA is closely related to changes in T-lymphocyte and B-lymphocyte, but isn′t obviously related in NK cells. So effect of EB virus in IM child have certain clinical values to judge the severity and prognosis.

Objective:To meet the requirements of hospital consultation management, promote the hospital consultation work in order to make the hospital a huge consultation data processing becomes simple, reduce the burden of management personnel and medical personnel management information system.Methods: Through the on-site investigation to collect the needs of the management personnel and the clinical front-line personnel, visit the application of other hospitals, and combine with the actual work, the development of suitable for hospital consultation management information system, the consultation for effective management and monitoring.Results: Based on the analysis of the needs of all kinds of personnel and consultation management information system, the basic function of the consultation management information system is realized.Conclusion: The realization of the consultation management information system can meet the needs of all kinds of people in the process of hospital consultation management, so as to meet the high efficiency management of hospital consultation, so as to improve the level of hospital information resource utilization.