Atrioventricular septal defect (AVSD)is a frequently diagnosed congenital heart malformation affecting the atrioventricular valves and septa. Heart transcription factors play fundamental roles in atrioventricular valves development and atrioventricular septa formation. Now cardiac transcription factors ( GATA4, NKX2. 5,TBX5 ,ZIC3)have been identified to involve in the pathogenesis of AVSD in humans. Their abnormal expression or function change may cause atrioventricular valve and septa developmental anomalies. Here we review advances in the understanding of the relationship between these transcription factors and AVSD.

By analyzing the current situation of TCM science popularization, We found that internet is serving as the most influential and rapid developing media at present. Therefore, a through excavation and utilization of internet as a tool for TCM science popularization will not only expand TCM science popularization platform, enhance mass foundation, promote mass understanding and recognition of TCM, but also meet the need of TCM self-development.

Object A method for the determination of daidzein, genistein, daidzin and genistin in soybean extract by RP HPLC was established Methods Zorbax SB C 18 column (4 6?15 mm, 5 ?m) was used Daidzein and genistein were separated with mobile phase Ⅰ∶methanol acetonitrile 0 1% phosphoric acid solution (30∶8∶62) Daidzin and genistin were separated with mobile phase Ⅱ∶methanol acetonitrie 0 1% phosphoric acid solution (16∶24∶60) The UV detection wavelength was 254 nm Results The average recoveries of daidzein, genistein, daidzin and genistin were 98 7%, 99 5%, 98 4%, 98 6% and the precision (RSD) were 1 4%, 0 9%, 0 9%, 1 7% (n=4) respectively Conclusion: The method was accurate, rapid and with good reproducibility

Objective: To study the protection for Compound Zigui Capsule on climacteric syndrome and the mechanism of the action, connected clinical application.Methods: Ovaritectomized rats were used as model of climacteric syndrome and to observed the effects of Compound Zigui Capsule on weight and the index of the organ of OVX rats. Bone metabolism of OVX rats was examined by single photon radionulide emission computed tomograpy.Results: Compound Zigui Capsule could antagonize an increase in body weight and atrophy of the uterus of OVX rats. The estrogenic content and phosphoric level in serum increased significantly. Results of bone metaboism examination showed that the radioactive cound/weight(c/w t) increased. Conclusion: Compound Zigui Capsule had markedly effect on improvement of endocrine disorder, increase of weight, atrophy of the sexual organs and osteopenia in OVX rats.

Objective:To observe the change of quotas such as vision acuity,fusion acuity etc after CDP-choline therapy,and evaluate comprehensively the curative effect and feasibility of it.Methods:Eighty-one amblyopic subjects were divided into 3 groups randomly:medical group with CDP-choline administration,combined group CDP-choline administration plus occlusion and meticulous work,occlusion group occlusion and meticulous work.Various kinds of side effects were carefully observed and all results were analyased statistically.Results:The appearance of the improvement of visual acuity in medical group was the earilest,but deteriorated gradually after 4 months.The combined group had improvements in all kinds of amblyopia,lasting throughout the observation period.Retina light sensitivity significantly increased in the medical group and combined group.The latency of the P 100 wave was not improved after the treatment in all of the three groups.Moreover,generalized tonic-clonic seizure was induced by CDP-choline administration in one subject.Conclusion:CDP-choline can improve the VA of amblyopic eyes,and raise the retina light sensitivity.CDP-choline treatment plus occlusion has a better curative effect.CDP-choline may induce seizure.

Objective:To investigate the distribution and regulation of uterine NK cells in mouse uterus during early pregnancy. Methods:Using a unique uNK cell marker,Dolichos biflorus agglutinin (DBA) lectin,to localize NK cells in uterus of normal pregnancy,delayed implantation and artificially induced decidualization. Results:DBA staining is mainly distributed in some vascular endothelial cells during embryo pre-implantation. In D5 of pregnancy after embryo implantation,NK cells were distributed in the stroma of uterine mesometrial pole,with round,immature and small lymphocyte-like cells. During D7 and D8 of pregnancy,the immunostaining results showed that uNK cells were increasingly accumulated in the mesometrial pole of implantation sites,with their morphology changing from small round cells with scattered cytoplasm to large and mature cells with heavy cytoplasmic granules. In the artificially induced decidualization model,the distribution and maturity of NK cells are similar to those of the normal pregnancy in the mouse uteri. Conclusion:The distribution and maturation of uterine NK cells are dependent of uterine decidualization in mouse early pregnancy.

Objective To review and analyze the causes of infant malnutrition and its relation with the way of feed.Methods Infant malnutrition was analysed with the emphasis on pathogeny,the onset month of the birth, its complication and clinical reflection in 63 cases.Results Infant malnutrition occurred in the case with incorrect feeding way:feeding on low protein powdered milk or cereals untimely without considering the supplementary food. Malnutrition easily brought out other kinds of disease.Conclusion It is recommended that infants younger than 5 months should be fed on breast-milk.In case of no breast-milk or short,dairy products are introduced,especially in- fant formula,with adding supplementary food timely.If not so,infant malnutrition occurs.

Objective To simulate the blood flow features of the susceptible sites of atherosclerosis in vitro.The cell micro-patterning is to be obtained on the bottom of channel for the information about single cell or cell groups.Methods Poly-dimethylsiloxane(PDMS) flow channel and PDMS stamp were fabricated by soft lithography.Fibronectin pattern was formed on chamber bottom by micro-contact printing method and cells selectively attached on fibronectin pattern.Results The PDMS-patterned chamber that possessed favorable optical property and the PDMS stamps with different sizes were obtained.Cells attached on fibronectin pattern presented square array.Conclusion The fabricated methods are quick and simple.The chamber could be used for the advanced real-time investigation of cell mechanical behavior in vitro.

Objective:To explore the clinical feature and prevention of delayed vitamin K deficiency in infants.Methods:The clinical data of 36 infants with delayed vitamin K deficiency who had been admitted to paediatric ward in our hospital during the last 4 years were analyzed retrospectively.Results:28 cases were cured with a cure rate of 77.78%;5 cases died with a mortality rate of13.89%;3 cases gave up treatment which accounts for 8.33%.Conclusion:The incidence rate of delayed vitamin K deficiency is high in infants on pure breastfeeding,and it could be prevented by periodical administration of supplementary vitamin K_1 in small doses to all infants from birth to 3 months.The early diagnosis and proper treatment of delayed vitamin K deficiency are of much clinical significance in increasing cure rate and reducing mortality rate.