Objective　To investigate the changes of free triiodothyronine (FT3) and its therapeutic effects on heart dysfunction after thoracic trauma.　 Methods　Eighteen rabbits were assigned randomly into 3 groups. The rabbits in Group A were served as a control, that in Group B were shot at the left thoracic wall passing through the cardiac region with small diameter rifle, that in Group C were treated by FT3 into veins (10 ng/kg) after thoracic trauma. 　Results　In Group B, MAP was much lower than that in Group A after trauma (P<0.05). In Group B, LVEDP and CVP respectively was much higher than that in Group A. Plasm concentration of cTnT in Group B was much higher than that in Group A. But level of FT3 was in Group B decreased more markedly than that in Group A after trauma. In myocardial interstitium, many red cells were leaked out of vessel. In Group C, MAP was much higher than that in Group B, no significant difference was found. CVP and LVEDP in Group C were much lower than that in Group B. Level of FT3 was near to normal and much higher than that in Group B. Edema of myocardial cells and hemorrhage of myocardial interstitium were alleviated markedly. 　Conclusions　Rabbits shot dead at the left thoracic wall passing through cardiac region with small diameter rifle can contribute to an animal model establishment of heart dysfunction. Reduction of FT3 is in correspondence with heart dysfunction. Correcting thyroid sick syndrome after trauma can improve significantly the heart function.

Objective To summarize the image feature of normal colorectal mucosa and colorectal cancer tissues based on high-resolution microendoscopy (HRME), and evaluate the feasibility of HRME in the diagnosis of colorectal cancer. Methods Twelve colorectal cancer surgical specimens confirmed by pathology and 56 biopsy specimens from colorectal lesions by conventional endoscopy were selected for the study. The normal mucosa and colorectal cancer tissue were imaging-observed to establish the HRME-diagnostic criteria of the normal mucosa and colorectal cancer tissues taking pathological diagnosis as the gold standard. According to the established criteria, the 56 biopsy specimens of colorectal lesions were HRME-imaged, and the prediagnosis was made. The prediagnosis result was compared with the pathological results for evaluating the diagnostic value of HRME. Results In the prospective study of 56 colorectal lesion specimens, 25 patients were diagnosed with cancer by HRME, of which 21 cases were confirmed by pathological examination. 31 specimens were diagnosed as non-cancerous tissues by HRME, and 29 were confirmed by pathology. The sensitivity, specificity, accuracy, positive predictive value, and negative predictive value of HRME in diagnosis of colorectal cancer were 91.3%, 87.9%, 89.3%, 84.0%, 93.5% respectively. The Kappa value was 0.78. Conclusions HRME can distinguish cancerous lesion from non-cancerous colon tissue, and have good consistency with pathological diagnosis, thus is of good prospects in terms of guidance for biopsy and diagnosis of colon cancer.

An important premise of Marxism is to attach importance to human health. Although Marx did not specially discuss health problems， many of his writings contain a rich view of health， which is an important part of the Marxist theoretical system. From the viewpoint of the formation mechanism， the emergence of Marx’s health thought is based on the historical development of the demand for the scientific view of health， developed in Marx’s in-depth thinking and research on historical materialism， sublimated in the need to guide the realistic movement of the working class at that time， and continuously enriched and developed based on practical experience. In terms of the composition of content， Marx’s health thought includes emphasis on workers’ health rights， concern for the public living environment， and the proposition that health is a necessary quality for people in future society. It provides ideological guidance for the cultivation of people’s view of health， theoretical enlightenment for the promotion of the construction of a healthy China， and a powerful weapon for resolving global health problems.

Objective To study the expression of receptor for advanced glycation end products ( RAGE ) in chronic adriamycin ( ADR )-induced cardiotoxicity mice . Method A mouse model of chronic cardiotoxicity was established by intraperitoneal injection of 5 mg/kg ADR once a week for continuous 3 weeks.Mice treated with the same volume of saline were used as control group (6 mice in each group).Echocardiography and histopathological examination were used to evaluate the myocardial injury , cardiac function and ventricular remodeling .The expression of RAGE in cardiac tissues was detected by immunohistochemical staining and Western blotting .Results Echocardiography indicated that ADR-treated mice had reduced left ventricular ejection fraction , while histopathological analysis of cardiac tissues showed obvious cardiac damage . RAGE protein in cardiac tissues was highly expressed in chronic ADR-induced cardiotoxicity mice .Conclusion RAGE is highly induced and may be involved in the cardiotoxicity after ADR treatment .

Objective To observe the effect of marrow mesenchymal stem cells(MSCs)transplantation combined with Qidantongmai tablet and simple MSCs transplantation on myocardial cells apoptosis and heart function in acute myocardial ischemia rats.Method Rat MSCs were isolated by density gradient method,and then cultured and amplified to the third passage labeled with BrdU.16 SD rats were divided into two groups randomly,transplantation combined with Qidantongmai tablet group and simple transplantation group.The isochoric of suspension of Qidantongmai tablet(2 g/kg)and NS were administered to SD rats by gastrogavage respectively for 14 days.The left anterior descending coronary artery of rat was ligated to establish animal models of acute myocardial infarction.The MSCs labeled with BrdU were implanted intramyocardially into the infarct area.After 3 hours,the heart function were detected by an echocardiographic system(HP 5500 system),equipped with a 12 MHz probe.After 4 weeks,the heart function were rechecked and MVD were detected by immunofluorescence,myocardial cells apoptosis were detected by TUNNEL.Results Heart function were improved in both groups,and was more significant in MSCs transplantation combined with Qidantongmai tablet group.Apoptotic index were decreased and the MVD of ischemic zone were increased in transplantation combined with Qidantongmai tablet group than simple transplantation group.Conclusion MSCs transplantation combined with Qidantongmai tablet could improve heart function of acute myocardial ischemia rats,decrease apoptosis of myocardial cells,increase the MVD of ischemic zone,the therapeutic effect were more significant than simple MSCs transplantation.

Objective To investigate the effect of Fingomod（FTY720）on the cerebral ischemia-reperfusion injury in rats. Methods SPF rats（250 g～300 g）of 8 weeks old were divided into sham operation group，blank control group，group of Clopidogrel and group of FTY720.Rat model of ischemic stroke was made by tMCAO. The behavioral disorder scores were scored in different groups after different drug treatments. And observing the area of infarctus tissue by the TTC method and the nerve cells changed by HE method in different groups. The levels of inflammatory factors IL-1β and IL-6 in brain tissue of rats were changed by method of ELISA. The level of p-p38MAPK and NF-κB in brain tissue of rats were determined by Western-blot method. Results（1）FTY720 can alleviate the behavior disorder of rats（P<0.01）；（2）FTY720 can reduce the area of infarctus tissue（P<0.01）and the injury of neurology cells in rats；（3）FTY720 can reduce the level of p-p38MAPK and NF-κB（P<0.01）in the ischemic brain tissue of rats；4，FTY720 can be reduced The level of IL-1β（P<0.01）and IL-6（P<0.01）in the ischemic brain tissue. Conclusion Fingomod（FTY720）has a protective effect on the cerebral ischemia-reperfusion rat model by decreasing the level of inflammatory response.

In the process of drug clinical trials， while proposing positive countermeasures to the legal and practical issues they raise， more attention should be paid to the ethical aspects of their responsibility， such as loss of the subjects’ sense of personal responsibility， insufficient supervision by regulatory bodies， responsible parties of researcher ignoring the spirit of the contract， and conflicts between professional requirements and individual requirements. In response to the above issues， based on the principle of responsible ethics， specific countermeasures were proposed， such as cultivating personal awareness of responsibility ethics， strengthening the standards of industry ethical review， improving informed consent compensation clauses， and cultivating research subjects’ awareness of responsibility ethics， thus to enhance the protection of the rights and interests of drug clinical trials’ subjects， as well as to protect the subjects’ rights and interests of life and health.

Palliative care， as the most special way to treat malignant tumors， is not only conducive to improving the quality of life and life value of patients， as well as alleviating the physical and mental pain of patients and their families， but also can help to relieve their financial burden and effectively avoid the waste of limited public resources. However， in the actual promotion of palliative care services， various ethical issues are encountered， such as the dignity of death， the need for psychological care， and the patient’s right to independent decision-making. From the perspective of bioethics， by analyzing different types of practical disputes， this paper revealed their inherent ethical problems， and proposed specific countermeasures from the aspects of defending patient dignity， breaking the limitations of traditional cognition， and establishing a field model for joint decision-making between doctors and patients， so as to build a palliative care service model that suits China’s national conditions and safeguard the patients’ life rights and interests with malignant tumors.

The study is designed to evaluate the protective effect of xanthan gum (XG) injection on cartilage injury in the rabbit osteoarthritis (OA) model induced by anterior cruciate ligament transection (ACLT), and to explore the effect of XG on the expression of caspase-3 and Bax protein in OA cartilage. Sixty male New Zealand white rabbits were randomly divided into 6 groups (n=10) according to random number table method, and one group was selected randomly as the normal control group (control) while the other 5 groups of right knee were used to establish the OA model with ACLT, which were then divided into model group (model), XG-0.6 mg·kg^-1, XG-1.2 mg·kg^-1, XG-2.4 mg·kg^-1 treatment group and sodium hyaluronate (SH-1.2 mg·kg^-1) treatment group according to drug intervention. The knee joint temperature and knee joint width of each group were measured in the course of treatment. After treatment, the macroscopic morphology of rabbit joints in each group was observed. The pathological morphology of articular cartilage of rabbits in each group was observed using HE staining. The expression of Bax and cleaved caspase-3 in the cartilage of rabbits were detected by Western blot. The result shows that XG inhibited the increase in knee joint temperature and knee width caused by OA in a dose-dependent manner. XG improved the morphological abnormalities and tissue injuries of the femoral condyle and tibial plateau caused by OA. Western blot result shows that, compared with the control group, the levels of Bax and cleaved caspase-3 in knee cartilage cells of model group and XG-0.6 mg·kg^-1 group were significantly increased (P<0.01). However, no significant difference was observed in the levels of Bax and cleaved caspase-3 between the model group and the XG-0.6 mg·kg^-1 group (P>0.05). These two groups are significantly higher than those of XG-1.2 mg·kg^-1 and XG-2.4 mg·kg^-1 (P<0.01) groups. Meanwhile, no significant difference was observed in the level of cleaved caspase-3 between the knee cartilage in XG-2.4 mg·kg^-1 and XG-1.2 mg·kg^-1 group (P>0.05). The level of Bax in knee cartilage in XG-2.4 mg·kg^-1 group was lower than that of XG-1.2 mg·kg^-1 group (P<0.05). In conclusion, XG effectively protected cartilage damage in OA, and inhibited the expression of Bax and caspase-3 protein in OA cartilage.

In view of the current norms and demands of human gene editing technology at home and abroad, the paper explained that the regulatory difficulties faced by human gene editing technology were due to the conflict between economic interests and moral bottom line by constructing a game model in a hypothetical way. On this basis, the ideas of the supervision mode of human gene editing technology were put forward: establish unified international standards based on the country as the main body, enact more stringent and effective laws, to jointly deal with the behavior of genetic manipulation of human gametes, zygotes and embryos for the purpose of reproductive, and ensure the normalization and legalization of gene editing technology to avoid technology abuse.