Cross-Cultural Comparison ; Humans ; Medicine, Chinese Traditional ; Terminology as Topic ; Translations

Objective To explore the effect of transforming growth factor ?_1 (TGF-?_1) on expression of paxillin(Pax) in human kidney proximal tubular epithelial cell line (HKC cells) in different phases. Methods HKC cells cultured in vitro were divided into four groups at random. Control group(C group): HKC cells were cultured with serum free medium (FSM), and TGF-?_1-treated groups (T1, T2, T3 group): HKC cells were cultured with FSM containing 10ng/ml TGF-?_1. In the latter groups, duration of treatment varied as follows: T1 group for 24h, T2 group for 48h, T3 group for 72h. The proliferation of HKC cells induced by TGF-?_1 was assessed by MTT and the expression of Pax was determined. The gene expression of Pax was determined by reverse transcripton polymerase chain reaction (RT-PCR) and the protein expression of Pax was assessed by immunohistochemical staining and Western blot. Results TGF-?_1 could induce the proliferation of HKC cells in a time-dependent manner. In C group there was expression of Pax mRNA and protein in HKC cells. The expression of Pax mRNA and protein in HKC cells was positive in all the T groups, especially in T2 group and T3 group they were significantly increased compared with T1 group (P

Objective To investigate the expression of Paxillin (Pax) in human kidney proximal tubular epithelial cell line (HKC) induced by transforming growth factor ?1(TGF?1). Methods HKC cells cultured in vitro were divided into three groups at random: control group(C): cultured with free serum medium(FSM), TGF?1-treated groups (T1 and T2): cultured with FSM containing different concentrations of TGF?1 (T1: 5 ng/ml, T2: 10 ng/ml). After 48-hour treatment, the expression of Pax mRNA and protein was detected by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical staining in HKC cells respectively. Results In Group C, the expression of Pax mRNA and protein in HKC cells was elementary. However, in T1 and T2 groups , the expression of Pax mRNA and protein in HKC cells was greatly increased, especially in T2 group which was more significantly increased than T1 group (P

Objective To investigate the clinical effect of Allevyn Thin on phlebitis caused by PICC. Methods Total 60 patients with phlebitis caused by PICC were randomly divided into 2 groups:experiment group and control group. The patients in the experiment group were treated with Allevyn Thin, while the patients in the control group were treated with hydropathic compress of 50% magnesium sulfate solution. The curative effect and healing time of the two methods were observed and compared. Results The average healing time of the experiment group was significantly shorter than that of the control group, and the curative effect of the experiment group was significantly better than that of the control group (P<0.05). Conclusion Allevyn Thin can shorten the healing time and improve the curative rate in treating phlebitis caused by PICC, suggesting that Allevyn Thin has a good potential of extensive clinical application.

Objective To explore the effects of resveratrol on the express of inflammatory factors (IL‐1 ,IL‐6 ,TNF‐α)of hu‐man umbilical vein endothelial cells(HUVEC) induced by Aβ1 -42 .Methods HUVEC were stimulated with Aβ1 -42 5 × 101 μmol/L , last 24 h and administrated with resveratrol 160 ,80 ,40 ,20 μg/L ,HUVEC viability were detected by CCK‐8 ;the concentration of IL‐1 ,IL‐6 and TNF‐α were detected by ELISA .Results Compared with model group ,160 ,80 ,40 ,20 μg/L resveratrol could in‐crease HUVEC survival rate ,reduced HUVEC damage by Aβ1 -42 ,inhibited the concentration of IL‐1 ,IL‐6 and TNF‐α(P<0 .05) . Conclusion Resveratrol over 20 μg/L could reduce the release of IL‐1 ,IL‐6 and TNF‐αof HUVEC inducing by Aβ1 -42 .

Objective To explore the effect of applying PDCA Circulation Theory in practical teaching of Basic Nursing at Higher Vocational College.Methods By using cluster random sampling method 2 classes of each 50 people from the nursing class of Grade 2013 were selected as the control group and the experimental group respectively.The traditional method of practice teaching was used in control group,that is,from the presentation of teachers,students practice,the guidance of teachers,periodic assessment to the final examination,while the PDCA Circulation Theory was used in experimental group.With periodic skill examination result,the final skill examination result and students' learning experience as evaluation index,two groups were compared with t test,using SPSS 17.0 for data analysis,expressed as percentage and-x ± s.Results The results of both single skill examination and the final skill examination as well as study experience of experimental group were higher than those of control group,and the differences are statistically significant.Conclusion Using PDCA Circulation Theory in practical teaching of Basic Nursing at Higher Vocational College can stimulate the students' consciousness and initiative,cultivate students' critical thinking,promote the student to study in a planned way with purpose and system and improve the learning effect.

Today,we are facing more and more needs for the protection of patients' privacy in the medical education.In order to avoid the invasion of patients' privacy and establish the harmonious professional-patient relationship,we should strengthen the consciousness of the law,set up the moral fibre,perfect the system for the protection of patients' privacy and enhance the training of the humanities to medical students.

At present higher education evaluation system in our country is gradually consummated and more and more significant, but the evaluation conclusion and its usage have not attained enough attention.This paper has appraised the formation and manifestation of conclusion of undergraduate teaching evaluation in Chinese common higher institutions,discussed the feasibility of evaluation conclusion in the government,universities and social public,and suggested how to greatly use the evaluation conclusion. Its purpose is to make more people put more attention to the usage of evaluation conclusion.

Objective To compare the results of blood glucose detected by the POCT glucose meters and the automatic biochem‐ical analyzer ,and to periodically evaluate the reliability of the portable glucose meters .Methods 22 patients with fasting blood‐glu‐cose of 0 .88 - 30 .82 mmol/L were selected and the NaF anticoagulation whole blood was collected .Each sample were detected for 3 times .The mean value was taken for conducting the bias and correlation evaluation .Results The bias of 20 glucose meters was within ± 0 .2 and PT > 80% ,and the bias of 5 glucose meters ± 0 .2 in 2 - 6 concentration values .Conclusion The testing results of 20 glucose meters are identical with those detected by the biochemical analyzer and meet the accuracy standards stipulated by the Ministry of Public Health ,and 5 glucose meters have relatively big differences .

Objective:To search for the efficient immune routes for rotavirus DNA vaccine by evaluating efficacy in inducing immune responses to rotavirus.Methods:In this study BALB/c mice were immunized by intramuscular and intranasal routes with rotavirus DNA vaccine,pcDNA 1/VP7,then rotavirus specific systemic immunity was measured.Results:Serum rotavirus specific IgG but not IgA titers of mice immunized by both routes were significantly higher than that of the unimmunized controls.Conclusion:Both intramuscular and intranasal routes are candidates for rotavirus DNA vaccine.