The purpose of foreign language teaching in Chinese medicine is not only translation,but also clearly the role and the purposes of translation.Inputing a culture to another culture is not culture exchange,but cultural coagulation.Expressions with modem scientific system allow speculative natural philosophy freed from the ancient Chinese-style paper into the theory expressed by modem scientific language in order to solve the problems of teaching foreign languages of traditional Chinese medicine texts.

Objective To synthesize an iron-based metal organic framework(MOF) material and investigate its adjuvant effect on immune response in mice. Methods The iron-based MOF material was synthesized via the hydrothermal reaction method, of which the morphology and composition were characterized by transmission electron microscope(TEM), scanning electron microscope(SEM), dynamic light scattering particle analyzer(DLS), X-ray photoelectron spectrometer(XPS), and specific surface area analysis(Brunauer Emmett Teller, BET). Using ovalbumin(OVA) as immunogen, 20 female BALB/c mice were randomly divided into iron-based MOF + OVA group(60 μg iron-based MOF + 20 μg OVA), Alu + OVA group(50 μg Alu+ 20 μg OVA), OVA group(20 μg OVA), and blank group(0. 9% normal saline), and were injected intramuscularly through the lateral thigh at 0, 14 and 28 d, respectively, with 100 μL/mouse. Blood samples were collected from the tail vein at 7, 21 and42 d after the initial immunization, the serum was separated, and the specific IgG antibody level was determined by ELISA.At 42 d after the initial immunization, the levels of specific IgG subtype antibodies in serum, IFNγ secreted by splenocytes,as well as IFNγ, IL-2 and IL-4 secreted by CD4~+T and CD8~+T cells were detected by ELISA, ELISPOT and flow cytometry,respectively. The body mass of mice at 0, 7, 14, 21, 28, 35, and 42 d after the initial immunization was measured, and the behavior of mice in various groups was observed. Results The synthesized iron-based MOF material exhibited a spherical morphology, an average particle size of approximately 129. 4 nm, a specific surface area of 16. 7 m~2/g and a pore diameter of7. 73 nm, containing C, N, S and Fe elements with more content of Fe element. At 42 d after the initial immunization, there was no significant difference in IgG level between iron-based MOF + OVA group and Alu + OVA group(F = 1. 00, P > 0. 05),but IgG2a and IgG2b levels increased significantly in iron-based MOF + OVA group(F = 16. 90 and 16. 57, respectively,each P < 0. 05);the level of IFNγ secreted by spleen cells increased significantly(F = 19. 47, each P < 0. 05);the level of IFNγ secreted by CD4~+T cells increased significantly(F = 14. 94, P < 0. 01), but that secreted by CD8~+T cells had no significant difference(F = 1. 00, P > 0. 05), and the levels of IL-2 and IL-4 secreted by CD8~+T cells increased significantly(F =100. 00 and 945. 80, each P < 0. 001). During the whole immunization process, there were no abnormal behaviors such as depilation or refusal to eat, and the body mass of mice in various groups changed little. Conclusion The prepared iron-based MOF material is simple to synthesize, stable in structure with nano-scale size, which can elicit humoral and cellular immune responses in mice, and is expected to become a new vaccine adjuvant.

PurposeTo explore the value of prenatal ultrasound diagnosis of pentalogy of Cantrell.Materials and Methods A retrospective study was performed in 6 fetuses with pentalogy of Cantrell diagnosed with prenatal ultrasound. The first diagnostic ultrasound time, ultrasound images and follow-up results were reviewed to analyze ultrasonographic features.Results All the fetuses were characterized by omphalocele and ectopic heart. All 6 fetuses were diagnosed by prenatal ultrasound during the first ultrasound screening, of which 5 were diagnosed before 16 weeks of gestation. Five cases were confirmed with induced labor and 1 was confirmed postnataly. The accuracy of prenatal ultrasonic diagnosis was 100%.Conclusion The characteristic features of pentalogy of Cantrell are omphalocele and ectopic heart. Prenatal ultrasound is reliable and valuable to diagnose pentalogy of Cantrell.

Objective To investigate the value of BISAP scoring system in predicting the severity of acute pancreatitis.Methods Medline,EMBASE,Science Direct,Springer link,CBM,Cnki,Wan fang and VIP database were retrieved by computer between January 2000 and March 2013,and articles of BISAP score system in predicting the acute pancreatitis were collected according to strict inclusion criteria.Quality assessment was made by QUADAS scale.Meta-Disc1.4 software was used to analyze the heterogeneity of included articles and perform quantitative synthesis,as well as calculate the pooled sensitivity and specificity,positive likelihood ratio,negative likelihood ratio,and draw the ROC curve,and the results were presented with 95％ CI.Results A total of 11 articles were included,and there were 7 Chinese articles and 4 English articles.According to QUADAS scale,there were 4 articles of A grade,5 articles of B grade,2 articles of C grade.There were 6 studies using 2 as BISAP cut-off value,and 9 articles using 3 as cut-off value,while 4 studies using 2 cut-off values.Pooled analysis showed diagnostic odds ratio of 8.03 (95 ％ CI 5.66-11.38) when cut-off value was 2,and diagnostic odds ratio of 7.49 (95％ CI 5.35-10.49) when cut-off value was 3.There were moderate heterogeneity in both groups (I2 =63.3 ％,P =0.018; I2 =56.1％,P =0.019).When BISAP cut-off value was 2,the pooled analysis of sensitivity,specificity,positive likelihood ratio,negative likelihood ratio and area under curve (AUC) were 59％ (95％ CI56％-63％),82％ (95％ CI 80％-83％),3.50 (95％ CI2.96-4.14),0.45 (95％ CI0.36-0.56) and0.82; and when BISAP cut-off value was 3,the corresponding values were 44％ (95％ CI41％-47％),90％ (95％ CI 89％-91％),4.59 (95％ CI 3.31-6.37),0.64 (95％ CI 0.61-0.68) and 0.64.The former had a high sensitivity,low specificity and large AUC,while the latter had a low sensitivity,high specificity and small AUC.Conclusions The best cut-off value of BISAP in predicting SAP is 2.When cut-off value is 2,the misdiagnosis rate is low,and the false positive rate is in the acceptable range,which is suitable for clinical application.

Based on the report of previous clinical study which showed cholinergic receptor antagonist scopolamine had antidepressant activity, this study was to investigate the antidepressant activity of scopolamine and explore its effective dose in mice, and to evaluate the effect of scopolamine on the central nervous system and learning/memory ability at its antidepressant effective dose. Tail suspension test, forced swimming test, step-down passive avoidance test and open field test were used to evaluate its effects on mice. Compared with the vehicle control group, single-dose administration of scopolamine (0.1-0.4 mg x kg(-1), ip) significantly decreased the immobility time (P < 0.01 or P < 0.001) in tail suspension test, and significantly decreased the immobility time (P < 0.001) in forced swimming test, but had no effect on the step-down latency and errors in step-down passive avoidance test. Scopolamine (0.1 and 0.2 mg x kg(-1), ip) had no influence on the locomotor activity in open field test, while at dose of 0.4 mg x kg(-1) significantly increase the locomotor activity. These results showed that scopolamine produced reliable antidepressant effect at doses of 0.1 and 0.2 mg x kg(-1), without impairment on learning and memory, as well as excitory or inhibitory effect on central nervous system in mice.

Research of oncogenes related to thyroid cancer has an important significance to reveal pathogenesis and identify benign and malignant lesions.At the mean time,these genes are helpful to find the reliable and feasible detection means of early diagnosis for thyroid cancer.

Objective To screen a thermostable urate oxidase-producing strain, optimize the fermentation conditions and study the enzymatic properties.Methods A urate oxidase-producing strain was screened from high temperature starter based on transparent circle method.Its 16S rDNA sequence was then amplified and analyzed.Meanwhile, the phylogenetic trees were built.Optimization of the fermentation conditions from this strain was carried out.The enzymatic properties of urate oxidase were studied.Results A urate oxidase-producing strain, named Bacillus subtilis ZX-6 by molecular identification, was obtained.The production of urate oxidase under the optimized conditons (135.9 U/L) was 133.7%higher than before.The optimum reaction temperature and pH were 45℃ and 7.6 respectively.The residual activity of urate oxidase at 37℃ for 48 h was still 17.2%.Conclusion The successful screening of a thermostable urate oxidase-producing strain and optimization of the fermentation conditions will lay a foundation for the further research.

BACKGROUND: The endothelial-mesenchymal transition is known to play a central role in the pathological process of heterotopic ossification. OBJECTIVE: To verify the inhibitory effect of SMAD7 on endothelial-mesenchymal transition such as myofibroblast transformation and to explore whether it is a potential target for heterotopic ossification.METHODS: A lentivirus overexpressing SMAD7 was contructed and the optimal titre and transfection efficiency in rat aortic endothelial cells were determined. The lentivirus was then injected into a rat model of Achilles tendon injury, while the controls were given the injection of normal saline. Expressions of endothelial and mesenchymal markers at the injured site were analyzed by quantitative PCR and western blot assay. The heterotopic ossification was observed radiologically and histologically.RESULTS AND CONCLUSION: Local injection of SMAD7-delivering lentivirus resulted in an upregulation of CD31 and VE-cadherin, and a downregulation of N-cadherin and vimentin, suggesting that the endothelial-mesenchymal transition is blocked due to local SMAD7 overexpression. The inhibitory effect became more evident at 10 weeks than at 6 weeks after modeling. Radiology and histological staining further confirmed that the ossified structures in the tendon tissue disappeared after injection of SMAD7-delivering lentivirus, as opposed to the control group. These data suggest that local overexpression of SMAD7 can prevent postoperative heterotopic ossification with no effect on wound healing.

The paper introduces the design idea of physical examination system in students recruitment of colleges and universities in Beijing Physical Examination Center, elaborates the functions of personnel examination, department examination, test results input, query statistics, etc.The application of this system could improve the quality of physical examination work, management level and work effi-ciency, summarizes the effect of informatization management in Beijing Physical Examination Center.

Objective To investigate the relationship between rs7903146-T/C polymorphism of transcription factor7-like 2 gene (TCF7L2) gene and gestational diabetes mellitus in Han population of Liaoning province.Methods The rs7903146-T/C polymorphism was detected in 115 healthy Han controls and 113 patients with gestational diabetes mellitus by using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.Results There were three genotypes:CC,CT,and TT; and their frequencies in control group and patients were 95.65％/84.96％,4.35％/15.04％,and 0/0,respectively.The allele frequency T of rs7903146 in control group and the patients was 2.17％ and 7.52％,respectively.There was statistical difference between two groups.Conclusions The rs7903146-T/C polymorphism of TCF7L2 might be associated with gestational diabetes mellitus in Liaoning Han population.