Objective To explore the effect of transforming growth factor ?_1 (TGF-?_1) on expression of paxillin(Pax) in human kidney proximal tubular epithelial cell line (HKC cells) in different phases. Methods HKC cells cultured in vitro were divided into four groups at random. Control group(C group): HKC cells were cultured with serum free medium (FSM), and TGF-?_1-treated groups (T1, T2, T3 group): HKC cells were cultured with FSM containing 10ng/ml TGF-?_1. In the latter groups, duration of treatment varied as follows: T1 group for 24h, T2 group for 48h, T3 group for 72h. The proliferation of HKC cells induced by TGF-?_1 was assessed by MTT and the expression of Pax was determined. The gene expression of Pax was determined by reverse transcripton polymerase chain reaction (RT-PCR) and the protein expression of Pax was assessed by immunohistochemical staining and Western blot. Results TGF-?_1 could induce the proliferation of HKC cells in a time-dependent manner. In C group there was expression of Pax mRNA and protein in HKC cells. The expression of Pax mRNA and protein in HKC cells was positive in all the T groups, especially in T2 group and T3 group they were significantly increased compared with T1 group (P

Objective To investigate the expression of Paxillin (Pax) in human kidney proximal tubular epithelial cell line (HKC) induced by transforming growth factor ?1(TGF?1). Methods HKC cells cultured in vitro were divided into three groups at random: control group(C): cultured with free serum medium(FSM), TGF?1-treated groups (T1 and T2): cultured with FSM containing different concentrations of TGF?1 (T1: 5 ng/ml, T2: 10 ng/ml). After 48-hour treatment, the expression of Pax mRNA and protein was detected by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical staining in HKC cells respectively. Results In Group C, the expression of Pax mRNA and protein in HKC cells was elementary. However, in T1 and T2 groups , the expression of Pax mRNA and protein in HKC cells was greatly increased, especially in T2 group which was more significantly increased than T1 group (P

Cross-Cultural Comparison ; Humans ; Medicine, Chinese Traditional ; Terminology as Topic ; Translations

Objective:Vasculogenic mimicry(VM) is a new microcirculation form of tumor cells which found resent years. This research observed whether gastric cancer SGC-7901 cells can form vasculogenic mimicry in 3-dimentional culture and had a primer examination on its mechanism. Methods:Put SGC-7901 cells in 3-dimentinal culture and observed if it could form vasculogenic mimicry. Immunohischemistry tested the MMP-2 and MT1-MMP level of 3-dimentional cultured SGC-7901 cells. Observe the effect of PI3K way inhibitor on MMP-2、MT1-MMP level and VM formation of gastric cancer cells. Results:Gastric cancer SGC-7901 cells can form VM in 3-demintional culture. MMP-2 and MT1-MMP highly expressed in gastric cancer cells that took part in the tube structure. PI3K way inhibitor decreased MT1-MMPmRNA and MMP-2 protein level and inhibited VM formation of SGC-7901cells. Conclusion:Gastric cancer SGC-7901 cells had the ability to form vasculogenic mimicry in vitro 3-dimentional culture.One of its mechanism was probably through decreasing MT1-MMP and MMP-2 level.

Objective:To search for the efficient immune routes for rotavirus DNA vaccine by evaluating efficacy in inducing immune responses to rotavirus.Methods:In this study BALB/c mice were immunized by intramuscular and intranasal routes with rotavirus DNA vaccine,pcDNA 1/VP7,then rotavirus specific systemic immunity was measured.Results:Serum rotavirus specific IgG but not IgA titers of mice immunized by both routes were significantly higher than that of the unimmunized controls.Conclusion:Both intramuscular and intranasal routes are candidates for rotavirus DNA vaccine.

This report is intended to study the changes of TxB2 and 6-keto-PGF1 ?(the stable metabolites of TxA2 and PGI2) in rabbits suffering from experimental decompression sickness (DCS).The prophylactic effect of indomethacin on acute DCS was also investigated. It was found that there was a relationship between Doppler ultrasonic signals and severity of DCS, and that in rabbits which were dying of DCS, TxB2 and 6-keto-PGF1? levels were higher than those of pre-compression controls (P

Objective To investigate the clinical use of low molecular weight heparin (LMWH) with thromboelastography (TEG)R time monitoring in malignant tumor patients, and guide clinical rational drug use. Methods 145 patients with malignant tumors were respectively first times and second times with low molecular weight heparin 4 h after venous blood determination of TEG R time and anti Xa activity, compare the effects of different doses of LMWH on TEG R time; the effects of different doses of LMWH against the activity of Xa; the correlation between the doses of LMWH at first time and TEG R time; the correlation between the doses of LMWH at first time and the activity of anti Xa; the correlation between TEG R time and the doses of LMWH at first time. Results Different doses of LMWH treatment had a significant effect on the TER G time (P < 0.05); no significant effect of different doses of LMWH against the activity of Xa; TEG R time with the first LMWH dose significantly correlated (P < 0.05); anti Xa activity and LMWH at the first time had no significant correlation between the dosage; no significant correlation with the anti Xa activity of TEG R time. Conclusion Using TEG R time to detect the clinical application of low molecular weight heparin is more sensitive and accurate compared with anti Xa activity detecting in patients with malignant tumor.

Objective To investigate the surgical treatment of congenital vertical talus (CVT). Methods Ten feet in six patients (4 girls and 2 boys) with CVT were reviewed. Four of the feet were associated with neuromuscular disorders(Ⅱ), five with malformation syndromes. one foot was idiopathic. The age at surgery ranged from 13 to 39 months with an average of 16.5 months. All were operated with a single-stage soft tissue procedures by using double of posterior and dorsal incisions. Elongation of tendo Achillis and capsulotomy of the ankle and subtalar joints were performed through a straight posterior incision. The dorsal approach was made over the anterior inferior ankle crease from the tip of fibula to talonavicular joint laterally. The extensor digitorum longus and peroneal tendons were lengthened, whereas the peroneus tertius was always tenotomized. The calcaneocuboid capsule, subtalar capsule, and talonavicular capsule also were released. The tibilis anterior tendon was placed through a drill hole in the neck of the talus. One K-wire was passed through the navicular and then retrograde across the talonavicular joint to maintain the reduced position. The other K-wire drove from the heel pad across the subtalar joint. A cast was applied with the ankle in slight plantarflexion for 3 months. Then special shoe was applied for 12 months. Results Six cases (10 feet) were available for follow-up from 6 to 33 months (mean, 23.1 months). The postoperative clinical outcome was evaluated by using a version of Adalaar score. All patients got good cosmetic appearance of the feet and the medial longitudinal arch of feet, had no prominent talar head, and had no the ankle and subtalar motion loss. The presence of mild hindfoot valgus on weight-bearing was noted in only two feet. Seven of ten feet were wearing special shoes. Radiographically, two parameters-talar and calcaneal axis-first metatarsal base angles (TAMBA and CAMBA) were measured from the lateral view of the feet. Preoperative and final follow-up radiographs were obtained. None had avascular necrosis of the talus. TAMBA changed from 66?(range, 50?-98?) to 1.6?(range, -11?-16?), CAMBA changed from 26.1?(range, 14?-60?) to -5.45?(range, -13?-3?). Conclusion Operative reduction has been advocated as the only effective treatment for CVT. It is the best choice to use a single-stage soft tissue surgery for infants with CVT. It is concluded that the results of this procedure are satisfactory.

Objective To compare the results of blood glucose detected by the POCT glucose meters and the automatic biochem‐ical analyzer ,and to periodically evaluate the reliability of the portable glucose meters .Methods 22 patients with fasting blood‐glu‐cose of 0 .88 - 30 .82 mmol/L were selected and the NaF anticoagulation whole blood was collected .Each sample were detected for 3 times .The mean value was taken for conducting the bias and correlation evaluation .Results The bias of 20 glucose meters was within ± 0 .2 and PT > 80% ,and the bias of 5 glucose meters ± 0 .2 in 2 - 6 concentration values .Conclusion The testing results of 20 glucose meters are identical with those detected by the biochemical analyzer and meet the accuracy standards stipulated by the Ministry of Public Health ,and 5 glucose meters have relatively big differences .

Today,we are facing more and more needs for the protection of patients' privacy in the medical education.In order to avoid the invasion of patients' privacy and establish the harmonious professional-patient relationship,we should strengthen the consciousness of the law,set up the moral fibre,perfect the system for the protection of patients' privacy and enhance the training of the humanities to medical students.