Objective To investigate the efficacy of Xuebijing injection on the septic shock and its impact on the patient's PCT and CRP.Methods According to the digital table,60 patients with septic shock were divided into two groups according to random number table methods.30 patients in the control group were only given conventional treatment,while the research group were used of Xuebijing injection in addition.The clinical efficacy was compared after treatment and the PCT and CRP levels were detected before and 7d after treatment.The days of hosoitalization were recorded.Results The total effective rate of the study group was 70％,significantly higher than 40％ of the control group's (x2 =5.45,P ＜ 0.05),and the study group's PCT and C RP levels were significantly lower than before (t =33.54,48.02,all P ＜ 0.05),after treatment 7d and the extent significantly greater than the control group (t =6.68,7.67,all P ＜ 0.05).Furthermore,the number of days of study group's hospitalization was significantly lower than that of control group(t =10.21,P ＜ 0.05).Conclusion Septic shock treated by Xuebijing injection has a significant effect as well as a greater impact on the PCT and CRP,which is worthy of clinical use.

Objective To investigate the role of cystatin C in hypertensive disorder complicating pregnancy.Methods Peripheral blood from 39 healthy pregnant women,23 patients with mild gestational hypertension ,25 patients with moderate gestational hypertension and 27 patients with severe gestational hypertension,CysC,BUN,UA and crea were determined .Resultss CysC ,BUN,UA and crea were higher in gestational hypertension group. CysC ,BUN,UA and crea were higher in severe gestational hypertension than mild and moderate gestational hypertension group.CysC had higher positive predictive ratio than UA for severe gestational hypertension.Conclusion CysC was a sensitive index to detect the early defection of kidney in severe gestational hypertension combining with other index such as BUN,UA and crea.

Objectives To localize the initiation site of the transcript driven by the cDNA corresponding to SARS - CoV 5 ' - UTR when it act as a promoter. Methods The plasmid pGI3 -5' - UTR, in which a reporter gene Luc was driven by the cDNA corresponding to SARS - CoV 5' -UTR, was transfected into HepG2 cells, and then the total RNA was extracted. Subsequently, the extracted total RNA was reverse - transcribed using a specific primer and subjected to two turns of semi - nested PCR. The PCR product was cloned into a T - vector for sequencing. Results A 440bps product was harvested by reverse - transcription and two turns of semi - nested PCR, and the 56th nucleotide of SARS - CoV 5 ' - UTR was found being the initiation site, followed downstream with a highly conserved transcription - regulating sequence (TRS). Conclusion The 56th nucleotide and its downstream TRS of SARS - CoV 5 ' - UTR is key roles in regulating gene transcription.

OBJECTIVE To find out the situation of peri-operational lower respiratory tract infection in inpatients with congenital heart disease of left to right shunt and provide evidence for clinical prevention and treatment.METHODS Peri-operational lower respiratory tract nosocomial infection in 2 564 cases of congenital heart disease with left to right shunt during Jan 2003 to Dec 2005 was analyzed retrospectively.RESULTS The incidence rate of lower respiratory tract infection was 11.27% among 2 564 cases.The incidence rate was 15.51% in patients less than 2 years old.The most common pathogen was Pseudomonas aeruginosa(18.60%).The death rate in cases with lower respiratory tract nosocomial infection was higher than these non-infection cases.CONCLUSIONS Pulmonary congestion,less than 2 years old,mechanical ventilation and antibiotics abuse are main causes of lower respiratory tract nosocomial infection in patients with congenital heart disease with left to right shunt.Nosocomial infection can increase death rate.

Objective To observe effects of BMP-4 gene combined with TGF-β1 in repairing rabbit femur defect resulted from firearm wound. Methods The femur defect was made with firearm steel ball. Plasmid encoded BMP-4 gene identified in vitro and TGF-β1 were injected into the tissue of upper, lower and central parts of the defects at the second week after wound. The mRNA and protein expressions of BMP-4 in vivo were detected by real time-polymerase chain reaction (RT-PCR) and Western blot. Activity of alkaline phosphatase (ALP) and calcium content were measured for evaluating osteogenetic ability. The process and quality of osteogenesis were determined by pathological and X-ray examinations. Results mRNA and protein of BMP-4 could continually express for six weeks in vivo after injection. Activity of ALP in the experimental group was increased to ( 13.17 ±0.51 ) U/100 ml at the 8th week, which was significantly higher than (8.77 ± 0.44) U/100 ml in the control group, indicating that osteogenetic ability was markedly enhanced, which accorded with determination of calcium content. Pathological observation and X-ray proved prominent improvement of osteogenesis, with a shorter time and better quality, in the experimental group. Conclusion BMP-4/TGF-β1 can promote repair of firearm femur defect.

Based on the report of previous clinical study which showed cholinergic receptor antagonist scopolamine had antidepressant activity, this study was to investigate the antidepressant activity of scopolamine and explore its effective dose in mice, and to evaluate the effect of scopolamine on the central nervous system and learning/memory ability at its antidepressant effective dose. Tail suspension test, forced swimming test, step-down passive avoidance test and open field test were used to evaluate its effects on mice. Compared with the vehicle control group, single-dose administration of scopolamine (0.1-0.4 mg x kg(-1), ip) significantly decreased the immobility time (P < 0.01 or P < 0.001) in tail suspension test, and significantly decreased the immobility time (P < 0.001) in forced swimming test, but had no effect on the step-down latency and errors in step-down passive avoidance test. Scopolamine (0.1 and 0.2 mg x kg(-1), ip) had no influence on the locomotor activity in open field test, while at dose of 0.4 mg x kg(-1) significantly increase the locomotor activity. These results showed that scopolamine produced reliable antidepressant effect at doses of 0.1 and 0.2 mg x kg(-1), without impairment on learning and memory, as well as excitory or inhibitory effect on central nervous system in mice.

Aiming at the problems of cosmetic elective courses teaching,this article discusses some effective teaching methods such as adjusting teaching contents according to students'interest and putting comprehensive examination and evaluation into practice through optimizing teaching means.These measures have improved the teaching quality and effect of the elective course and meanwhile are beneficial attempts for the quality education

It has been suggested that some unusual molecules and genes might be involved in the development of soft tissue sarcomas (STS) and that drugs targeting specific molecules and genes would selectively kill tumor cells, which have been demonstrated to be more effective approach with less side effect than traditional chemotherapy. However, most of the effective targets of STS are currently not elucidated, and the effects of trial-target therapy are mostly not satisfactory, thus multiple targeting drugs might guide a future direction for tumor therapy.

Objective To explore the curative effect of anterior and posterior wall of the uterus through suture combined with intrauterine balloon compression and uterine artery ascending ligation to treat the hemorrhage of the placenta.Methods In June 2015 to June 2017 in our hospital 60 cases of placenta previa during cesarean section hemorrhage patients, were randomly divided into treatment group and control group , the control of intrauterine balloon compression and ascending branch of uterine artery using the group of patients, patients in the treatment group in the control group based method on the back wall of the lower uterine segment through suture in the treatment of. The curative effect and operation of the two groups were compared.Results The total effective rate was higher in the treatment group than the control group(P<0.05). The intraoperative blood loss, postoperative 24h blood loss of the treatment group patients were lower than the control group, the treatment group patients with operation time, length of hospital stay were lower than the control group(P<0.05).Conclusion The lower uterine segment through anterior and posterior wall suture combined with intrauterine balloon compression and ascending branch of uterine artery ligation is an effective method in treatment of hemorrhage in cesarean section for placenta previa, facilitate clinical application.

Objective Human amniotic mesenchymal stem cells (AM-MSCs) transplantation and to investigate the effect of propofol treatment, recovery of brain injury in rats. Methods 80 SD rats, male and female, weight 300-350 g. Human amniotic mesenchymal stem cells cultured in vitro and recovery (CM-Dil) before transplantation standby. The fluid percussion brain injury device, give 2.5-3.0 ATM hydraulic impact, severe fluid percussion brain injury model was made. After injury 6h give corresponding treatment which were randomly divided into 4 groups: injury group (medium injection group), AM-MSCs transplantation group and propofol group, AM-MSCs + propofol group. The number of positive cells in each group was observed under the fluorescence microscope using CM-Dil markers. 4 weeks after transplantation, 6 rats were randomly executed, and RT-PCR and Blot Western were used to detect the changes of GAP-43, AQP4 gene expression and protein synthesis in brain tissues. After transplantation, 24 h, 3 days and 1, 2, 3, 4 weeks for animal neurological deficit score, in 4 weeks after transplantation of Morris water maze (Morris Water Maze, MWM) test. They were killed after four weeks by immunohistochemistry, HE staining. Results Number of CM-DIL positive cell was the most in AM-MSCs + propofol group, which was in propofol group and AM-MSCs transplantation group were lower than that in the AM-MSCs + propofol group, which in injury group was the least, and the differences among the groups had statistically significant (P<0.05). 4 weeks after transplantation, AQP4 and its mRNA expression of brain damage surrounding tissue in injury group were higher than those in AM-MSCs transplantation group and propofol group; which in AM-MSCs transplantation group and propofol group was higher than those in AM-MSCs + propofol group (P< 0.05); GAP-43 protein and mRNA expression in injury group were lower than those in AM-MSCs transplantation group and propofol group, which in AM-MSCs transplantation group and propofol group were lower than those in AM-MSCs + propofol group (P<0.05). 1 weeks after transplantation, rat nerve dysfunction score in AM-MSCs + propofol group was lower than that in propofol group and AM-MSCs transplantation group; which in propofol group and AM-MSCs transplantation group was lower than that in the control group (P<0.05). The times of crossing platform in AM-MSCs + propofol group was higher than that in propofol group and AM-MSCs transplantation group, significantly higher than that in the injury group (P<0.01). The pathological section was observed 4 weeks after injury, there was no axonal passage in the injury group. In propofol group and AM-MSCs transplantation group, a few axonal like structures were observed, more axonal like structures were observed in AM-MSCs + propofol group. Conclusion Propofol combined with human amniotic mesenchymal stem cells transplantation can significantly improve the neurological function of rats with brain injury.