Acute Coronary Syndrome ; metabolism ; pathology ; physiopathology ; Cholesterol ; metabolism ; Erythrocyte Membrane ; metabolism ; Humans

Caspase 3 ; metabolism ; Child ; Female ; Glomerulonephritis, Membranous ; enzymology ; etiology ; pathology ; virology ; Hepatitis B ; complications ; pathology ; Hepatitis B virus ; Humans ; Male

Alzheimer Disease ; immunology ; therapy ; Amyloid beta-Peptides ; antagonists & inhibitors ; immunology ; metabolism ; Animals ; Antibodies, Monoclonal ; therapeutic use ; Blood-Brain Barrier ; Brain ; drug effects ; immunology ; Enzyme Inhibitors ; therapeutic use ; Humans

OBJECTIVETo study the effects of transcutaneous electrical nerve stimulation on multimodal analgesia after total knee arthroplasty.

METHODSSixty patients diagnosed as knee osteoarthritis and suffered unilateral total knee arthroplasty in the department of orthopedics, Zhejiang Traditional Chinese Medicine Hospital from March 2009 to May 2012 were randomly divided into control group and test group, 30 cases in each group. All the patients received celecoxib preoperatively, knee periarticular injection with the drug mixture intraoperatively, and celecoxib and morphine sulfate controlled-release tablets postoperatively. The patients in the test group were also treated with transcutaneous electrical nerve stimulation each day after operation. All the patients started doing functional exercises at 24 h after operation. Postoperative visual analogue scales(VAS), passive and active range of motion of knee joint,and complications were recorded.

RESULTSThe VAS scores of test group during postoperative 24 h to 1 week were 3.39 +/- 0.69, 2.79 +/- 0.51, 2.16 +/- 0.52, and 1.07 +/- 0.57 separately,which were lower than 3.80 +/- 0.86, 3.22 +/- 0.58, 2.53 +/- 0.54 and 1.38 +/- 0.52 in the control group. The passive and active range of knee joint motion in the test group during postoperative 24 h to 2 weeks were (30.67 +/- 3.65) degrees, (39.17 +/- 3.96) degrees, (47.83 +/- 4.86) degrees, (93.67 +/- 7.30) degrees, (107.67 +/- 7.51) degrees and (29.83 +/- 5.33) degrees, (78.33 +/- 8.24) degrees, (95.17 +/- 5.94) degrees respectively,which were higher than (28.67 +/- 3.92) degrees, (36.83 +/- 4.25) degrees, (45.17 +/- 5.17) degrees, (89.83 +/- 7.25) degrees, (103.17 +/- 7.37) degrees and (24.17 +/- 10.26) degrees, (73.83 +/- 9.07) degrees, (91.33 +/- 7.42) degrees in the control group. In the test group, 19 patients had ring-shaped haemostasis impression to some extent in the skin of knee joint to different degree, and 5 patients had blister formation.

CONCLUSIONIt is meaningful to apply transcutaneous electrical nerve stimulation for treating multimodal analgesia after total knee arthroplasty,which can not only relieve patients' postoperative pain, and also promote the rehabilitation of knee function as far as possible.

Aged ; Aged, 80 and over ; Analgesia ; Arthroplasty, Replacement, Knee ; adverse effects ; Female ; Humans ; Knee Joint ; surgery ; Male ; Middle Aged ; Pain, Postoperative ; etiology ; therapy ; Transcutaneous Electric Nerve Stimulation ; Treatment Outcome

Objective To observe the expressions of stem cell factor(SCF) and macrophage colony-stimulating factor(M-CSF) in bronchoaveolar lavage fluid(BALF) of asthmatic mouse,and the effect of bacillus calmette-guerin(BCG) intervention on them.Methods Thirty Kunming rats were randomly divided into asthmatic group,BCG intervention group and control group,each group had 10 rats.Mice were sensitized and challenged with ovalbumin (OVA) to establish asthmatic model.Asthmatic mice were injected intradermally with BCG on 7,3,1 d before sensitization.After 24 h of last challenge,BALF were collected.The total cells and eosinophils(EOS) were counted in the BALF.The SCF and M-CSF levels of BALF were determined by enzyme-linked immunosorbent assay(ELISA).Results The number of total cells and EOS [(27.06?4.25)?107 L-1,(7.58?1.30)?107 L-1]in BALF in asthmatic mice were more than those in control group[(4.93?1.43)?107 L-1,0](Pa0.05).The M-CSF level also increased noticeably in asthmatic mice[(204.30?10.39) ng/L] compared with the control group[(181.33?8.63) ng/L](P

Objective To explore the effect of budesonide(BUD) on dendritic cell(DC) and airway inflammation in the asthmatic mice.Methods Forty female Kunming mice were divided into 4 groups:asthmatic model group,therapeutic control group,BUD treated group,and normal control group,with 10 mice in each group.Mice were sensitized by an intraperitoneal injection of 50 ?g ovalbumin(OVA) adsorbed to 1 mg aluminum hydroxide dissolved in 0.2 mL saline.Animals were boosted on the 14th day in the same way.From the 21th to 35th days,and mice were challenged with 10 g/L aerosolized OVA for 30 min a day to establish a murine model of asthma.To evaluate the effect of BUD,60 minutes prior to OVA exposure,the mice were treated with 1 mg aerosolized BUD or placebo(saline).Control animals were sensitized intraperitoneally with saline and challenged with aerosolized saline alone.Eosinophil(EOS) count,degree of mucus secretion and DC count around the airways were measured by haematoxylin and eosin staining,periodic acid schiff's staining,immunochemistry technique and computerized image analysis system.Results In asthmatic model group,EOS count,DC count and the degree of mucus secretion around the airways were increased compared with nomal control group(P_a0.05).In BUD treated group,EOS count,DC count and the degree of mucus secretion around the airways were decreased compared with the asthmatic model group(P_a

The academic connotation of "pathogenic factors" is discussed in this article from three aspects of etiology,diagnosis and treatment.It is considered that endogenous or exogenous pathogenic factors oppose to and destruct healthy energy.TCM pathogenic factors,obtained from "examining syndromes for causes of diseases",are diverged from physical,chemical and biological those in modern medicine.What is meant by "therapy of expelling pathogenic factors" is not a single method of dispelling toxin and killing germs,but the resort of regulating the integrated abilities of the human body fighting against pathogenic factors.