Objective: To investigate the effect of levocarnitine on contrast-induced nephropathy (CIN) in coronary artery disease (CAD) patients after percutaneous coronary intervention (PCI).
Methods: A total of 138 CAD patients with PCI were studied. Upon basic hydration, the patients were divided into 2 groups:Levocarnitine group, the patients received intravenous infusion of levocarnitine 2g at 24h before PCI and levocarnitine 2g/day after PCI for 4 days and Non-levocarnitine group. n=69 in each group. Blood levels of urea nitrogen (BUN), creatinine (Scr), creatinine clearance rate (Ccr) and the occurrence rate of CIN were compared between 2 groups at 24h and 72h after PCI. All contrast agent used in the operation was Ultravist non ionic low permeability contrast solution and the dosage was recorded during operation.
Results: Compared with Non-levocarnitine group, Levocarnitine group had decreased blood levels of BUN and Scr at 24h and 72h after operation;increased post-operative Ccr, all P<0.05;the occurrence rate of CIN in Levocarnitine group was lower than Non-levocarnitine group (5.80%vs 15.94%), P<0.05.
Conclusion: Application of levocarnitine has certain preventive and protective effect for CIN occurrence in CAD patients after PCI.

Objective To observe the clinical efficacy of thread-embedding at scalp points in treating insomnia.Method Sixty-four insomnia patients were randomized into a treatment group of 33 cases and a control group of 31 cases. The treatment group was intervened by thread-embedding at scalp points, while the control group was by orally taking Estazolam. Four weeks were taken as a treatment course, and the clinical efficacies and changes of indexes were measured after a treatment course.Result The total effective rate was 90.0% in the treatment group versus 90.3% in the control group, and the difference was statistically insignificant (P>0.05). The item scores of Pittsburgh Sleep Quality Index (PSQI) were changed significantly after treatment in both groups (P<0.01). The changes of sleep latency and daily function disturbance in the treatment group were significantly different from that in the control group (P<0.05).Conclusion Thread-embedding at scalp points and oral administration of Estazolam both can improve insomnia symptoms. Thread-embedding at scalp points is inferior to Estazolam in improving sleep latency but is superior to Estazolam in improving daily function.

Objective To observe the effect of penetrating needling with thick needle at Shenzhu (GB 12) on the Unified Parkinson’s Disease Rating Scale (UPDRS) score in patients with Parkinson’s Disease (PD). Methods Sixty-one PD patients were randomized into a treatment group of 31 cases and a control group of 30 cases. The treatment group was intervened by penetrating needling with thick needle at Shenzhu (GV 12) in addition to oral administration of Levodopa and Benserazide Hydrochloride;while the control group was by oral administration of Levodopa and Benserazide Hydrochloride alone. The UPDRS was evaluated before and after intervention in both groups. Results Respectively after 30-day treatment, 90-day treatment, and 90 d after the intervention, the UPDRS scores were significantly changed compared to that before intervention in both groups (P<0.01, P<0.05);there were also significant differences in comparing the UPDRS score between the two groups at each time point (P<0.01, P<0.05). Conclusions Penetrating needling with thick needle at Shenzhu (GV 12) can improve the UPDRS score in PD patients, and it’s an effective method in treating PD.

Immune thrombocytopenia (ITP), the most common hemorrhagic disease, is an organ-specific autoimmune disease characterized by decreased platelets count due to auto-antibodies mediating platelets destruction and insufficient platelets production. The etiology of ITP is still not completely known. Regulatory T cells, also called Tregs, are character-ized by CD4+CD25+, and positive of transcription factor forkhead box P3. They belong to a subpopulation of T cells special-ized for immune regulation and play an important role in maintaining the immune balance. Decreased production and defect-ed function of CD4+CD25+Treg was found not only in the ITP animal model but also in the ITP patients. It indicates that the Treg was involved in the pathology of ITP. This review focus on the characteristic and function of Tregs and their relationship with pathogenesis of ITP.

Objective] To study the effect of 50 hz frequency of electricity stimulating finger extensor tendon for hand dysfunction caused by incident storke. [Methods] 67 cases diagnosed as hand dysfunction of incident storke patients randomly divided into treatment group and control group(33 cases in treatment group, 34 cases in control group). The control group was given Bobath method for hand function recovery training. The treatment group was given 50 hz frequency of electricity stimulating finger extensor tendon on the basis of Bobath rehabilitation treatment, 5 times every week, for 4 weeks. Evaluate patients' symptoms improvement, before and after, with Fugl-Meyer grade(hand part) and modified Barthel index evaluation, and then make statistical analysis of the results. [Result] After the treatment,the grades, Fugl-Meyer grade(hand part) and modified Barthel index, of two groups both increased significantly, with statistical significance(P<0.01); Both of the grades of treatment group were significantly higher than that of control group, with statistical significance(P<0.01). [Conclusion] 50 hz electrical stimulating finger extensor tendon can significantly improve hand dysfunction caused by incident storke, improve their abilities of daily life.

Objective To evaluate the expression of immunohistochemistry markers p53, p16 and Ki-67 in penile cancer and penile intraepithelial neoplasia (PIN). Methods 33 patients who suffered penile cancer and received organ-sparing surgery from Jan 2004 to Dec 2013 were retrospectively analysed. The expression of p53, p16 and Ki-67 were detected by EnVision/HRP two steps immunohistochemistry. Results The positive expression rates of p53 in normal penile epithelium, low-grate penile intraepithelial neoplasia, high-grate penile intraepithelial neoplasia, well-differentiated squamous cell carcinoma and poor-differentiated squamous cell carcinoma were 0, 16.7 %, 38.5 %, 30.0 % and 66.7 % respectively. The expression rates of p16 were 20.0 %, 50.0 %, 53.8 %, 70.0 % and 33.3 % respectively. The expression rates of Ki-67 were 0, 16.7 %, 61.5 %, 65.0 % and 75.0 % respectively. Conclusions The combined detection of p16 and Ki-67 can contribute to discover PIN and penile carcinoma much more earlier. p53 may be related to the occurrence and development of low differentiated squamous cell carcinoma, and p16 may be related to the occurrence and development of well-differentiated squamous cell carcinoma.

Full ceramic restorations become more and more popular for their excellent properties. Bond strength between ceramic and composite resin cement is the key factor to success in clinical use. This paper illuminated the importance of ceramic surface treatments and reviewed recent progresses on ceramic surface treatments. The difference of bond strength caused by different dental materials was discussed too.

Objective Obese patients with type 2 diabetes mellitus ( T2DM) characterized by hyperglycemia are li-able to more severe myocardial infarction .Semen Cassiae is proved to reduce serum lipid level .This study was to investigate whether the Semen Cassiae extract (SCE) reduces myocardial ischemia and reperfusion (MI/R) injury with or without diabetes and the underlying mechanisms .Methods The high-fat diet-fed streptozotocin ( HFD-STZ) rat model was used as T2DM model.Normal and DM rats received SCE treatment orally (10 mg/kg/day) for 1 week.Subsequently these animals were subjected to MI /R.Results Compared with the normal animals , DM rats showed increased plasma total cholesterol ( TC) and triacylglycerol ( TG) , more severe MI/R injury and cardi-ac functional impairment .SCE treatment significantly reduced the plasma TC and TG , improved the instantaneous first derivation of left ventricle pressure and reduced infarct size , decreased plasma creatine kinase and lactate dehydrogenase levels, and reduced apoptosis index at the end of reperfusion in diabetic rats .Moreover, SCE treat-ment increased the antiapoptotic protein Akt and stimulated ERK 1/2 phosphorylation .Pretreatment with a PI3 K in-hibitor wortmannin or an ERK1/2 inhibitor PD98059 not only blocked Akt and ERK1/2 phosphorylation, but also inhibited the cardioprotective effects of SCE .However , SCE treatment did not show any effects on the MI/R injury in the normal rats.Conclusions SCE effectively improves myocardial function and reduces MI/R-induced injury in diabetic but not normal animals , which is potentially attributable to the reduced TC/TG levels and the triggered cell survival signaling Akt and ERK 1/2 .

With the pluralistic need of medical service in the country,non-technical ser-vice has become the crucial part of core competencies of the medical institution.Therefore,pro-fessional ethics is considered the most important by the units in employing new members.It’s necessary to train students with good morality by combining the moral education with profes-sional education so as to meet the changes in the social needs.

ObjectiveTo study the effects of Pulse Magnetic Needle Apparatus on nerve growth factor(NGF) expression in cerebral ischemia model of rats.Methods70 Wistar rats were divided randomly into 7 groups as normal group,sham group,model group,magnetic group,acupuncture group,elecupuncture group and pulse magnetic group.Cerebral ischemia model was made by middle cerebral artery occlusion.The neural function of all the rats after operation was assessed by Nervous Scale of Function.The expression of NGF in each group was determined using immunohistochemical method.ResultsIn the Nervous Scale of Function between 6h and 15d after operation,there was no difference in model group(P>0.05),but there was difference in magnetic group(P<0.05) and in other therapeutic groups(P<0.01). The curative effect in pulse magnetic group was as the same as that in elecupuncture group and acupuncture group on the 15th day after operation.The expression of NGF increased in all therapeutic groups compared with the model group on the 15th day after operation(P<0.05 or 0.01).Furthermore, the expression of NGF increased in pulse magnetic group and acupuncture group rather than that in magnetic group and elecupuncture group(P<0.05).There was no distinct difference between acupuncture group and pulse magnetic group(P>0.05).ConclusionsThe Pulse Magnetic Needle Apparatus can promote and prolong the expression of NGF and recover the function of limbs.