OBJECTIVE:To observe the application of sevoflurane combined with different dose of fentanyl in general anesthesia-inducing endotracheal intubation in adults. METHODS:60 selective patients were randomly divided into three groups(n=20). In haled 8% sevoflurane inductal anesthesia with tidal volume method. When the patients breathing and his own,and loss of eyelvaisah i .rve.f lTehxe, SgBroPu,p DⅠBwP earne dg iHvRen in2 e?agc?hk ggr-o1 ufpe nwtaenrye l dveitae cit.evd., agt rtoimupe Ⅱof 3lo s?sg-? okf-g -ey1 efleanstha nryelf lvexia ain.vd. baenfdo rger oinudpu Ⅲcti o4n ?agn?esktgh-e1s ifae(ntTan0)y,l before intubation(T1),at the moment of intubation(T2),at 1 min after intubation(T3),3 min after intubation(T4),5 min after intubation(T5)and 10 min after intubation(T6). RESULTS:Compared with at T0,SBP,DBP and HR in three groups were significantly decreased at T1(P0.05). SBP, fDeBntPa naynld i sH eRff einc tigvreo utop iⅢnd uwceer ea ndeescthreeassiae di nm aadrukletd elyn daot trTa(2chPe

Objective To explore clinical value of magnetic resonance imaging (MRI) in preoperative risk assessment and choice of operation scheme for knee cartilage injury.Methods A retrospective study was conducted of the 69 cases of knee cartilage injury which had been confirmed by surgery between January 2013 and June 2014.They were 29 males and 40 females,aged from 15 to 76 years (average,46.9 years).All cases underwent preoperative 1.5 T MRI by a special diagnostic instrument for limbs.The injury was assessed after MRI according to the Outerbridge criteria to evaluate surgical risks and choose an appropriate operation scheme (drilling,micro-fracture or autoallergic cartilage grafting).The intraoperative observations were compared with the preoperative images.Results MRI found in the 69 patients 345 cartilage surfaces,of which 208 were abnormal.Of the abnormal ones,75 (21.7％) were grade Ⅰ which presented with intact or slightly eminent contour and dots or patches of low or high signals in the cartilage;39 (11.3％) were grade Ⅱ which presented with thinned cartilage,defects ＜ 50％ of the full thickness,or (and) indented or wavy cartilage surface,dots or patches of low or high signals in the cartilage,and dropsy signals in cavity;43 (12.5％) were grade Ⅲ which obviously thinned cartilage,defects ＞ 50％ of the full thickness,and massive dropsy signals in cavity;51 (14.8％) were grade Ⅳ which presented with full thickness defects of cartilage,subchondral bone exposure beneath the defcts,and abnormal adjacent tissues.The preoperative findings were in agreement with what were observed during surgery in terms of number and extent of cartilage injury.Conclusion Because MRI can exactly display knee cartilage injury in terms of location,degree,and size,it provides solid evidence for preoperative risk assessment and choice of operation scheme for knee cartilage injury.

0.05).CONCLUSION:Neuromuscular blockade induced by single i.v.of Cisatracurium showed no gender difference.

Objective: To better understand the function of mast cells in acupuncture points (acupoints) inacupuncture-induced analgesia. The author tested their sensitivity to mechanical, thermo and light stimulation.Methods: The tail flick model was applied to measure analgesia in rats, and the author determined the density of mast cells in tissue slices and their degranulation ratio before/after acupuncture. The author also applied the patch-clamp technique to investigate activation of human mast cells (HMC 1 cell line) by mechanical stress or noxious heat, and the author optically observed degranulation phenomena of mast cell in response to red laser light.Results: Manual stimulation by acupuncture at Zusanli (ST 36) of the rat resulted in analgesia and the effect was more pronounced than after stimulation of a sham point nearby the acupuncture point. A higher density of mast cells was found at Zusanli (ST 36) than at the sham point, and acupuncture caused a remarkable increase in degranulation. Pretreatment with disodinm chromoglycate (DSCG, a mast cell stabilizer) injected into Zusanli (ST 36) impaired the analgesic effect of acupuncture. In whole-cell patch-clamp, increasing hydrostatic pressure induced a current that could be inhibited by 10 ～tM of ruthenium red (RuR), an inhibitor of TRPV2. Temperatures above 50～C, which are reached during moxibustion, induced a similar RuR-sensitive current. Laser light of 640 nm (48 mW) applied to acupoints had been shown previously to be highly effective in analgesia. 20 min of light application induced pronounced degranulation in HMC 1 that could be blocked by 0.02 g/mL DSCG as well as by RuR. Conclusion: The author found that mechanical stimulation of acupoints is associated with mast cell degranulation and mast-cell degranulation can be correlated with acupuncture-induced analgesia. The author suggest that TRPV2 plays a key role in mast-ceU degranulation in response to mechanical, thermal (moxibustion) as well as laser-light stimulation forming a facilitating step in acupuncture-induced analgesia.

The content of zinc and copper in the hair of 321 persons was detected by an atomic absorption spectropolarimeter. The subjects included young healthy group (35 men, 30 women), middle aged healthy group (72 men, 18 women), arteriosclerosis group (81 men, 20 women), cerebral embolism group (23 men, 8 women) and its control group (23 men, 8 women). The ratio of zinc to copper in the hair of the persons suffered from cerebral embolism was obviously higher than that of healthy controls. By comparing the function of the susceptible factors in cerebral embolism with Feisher's determination analysis method, diastolic pressure was found to be 75.02 percent, the ratio of zinc to copper 16.32 percent. The diagnostic coincidence was found to be above 82 percent.

0.05).The prevalence of strains with resistance to rifampin was lower than that of strains with resistance to clarithromycin(P

Three plasmids (pGenesil-P1, pGenesil-P2, pGenesil-P3) with different p27Kip1-shRNA sequences were designed and synthesized. Their effects on the proliferation of bovine corneal endothelial cells (bCEC) were investigated. Plasmid expressing irrelevant shRNA with a random combination was used as negative control (pGenesil-HK). The recombination of four plamids was confirmed by restrictive enzyme digestion and sequence analysis. The expression of mRNA and protein of p27Kip1 was detected by RT-PCR and Western blotting after stable transfection. The expressions of p27Kip1 mRNA and p27Kip1 protein of pGenesil-P1 group, pGenesil-P2 group and pGenesil-P3 group were all lower than those in the pGenesil-HK group and the blank group (non-transfected group). pGenesil-P3 had the strongest inhibitory effect and was selected for the next steps. The proliferation rates of the pGenesil-P3 group, the pGenesil-HK group and the blank group were assessed by MTT. The influence of shRNA-p27Kip1 on bCEC cell cycle was detected by flow cytometry (FCM). Compared with the control groups, the proliferation rate of the pGenesil-P3 group was increased significantly, and the ratio of S-phase also increased. It is concluded that shRNA-p27Kip1 could down-regulate the expression of p27Kip1 effectively and increase the proliferation of bCEC. RNA interference (RNAi) may be an effective means to promote the proliferation of CEC.
Cell Proliferation ; Cornea/cytology ; Cyclin-Dependent Kinase Inhibitor p27/*metabolism ; Endothelial Cells/*cytology ; Endothelial Cells/metabolism ; Gene Expression Regulation ; Models, Biological ; Plasmids/metabolism ; RNA Interference ; RNA, Messenger/metabolism ; RNA, Small Interfering/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Tetrazolium Salts/pharmacology ; Thiazoles/pharmacology ; Transfection

Objective To better understand the clinical characteristics of Familial Idiopathic Basal Ganglia Calcifi?cation (FIBGC), including at the perspective of hereditary pattern, clinical test results, onset age, clinical heterogeneity and the volume of basal ganglia calcification (VBGC). Method 8 Eight FIBGC families were collected and draw family pedigrees were draw. Analysis of was conducted on the patient's clinical test results, head CT and MRI changes, onset ag?es, relationship of clinical manifestations with VBGC. Results No significant difference was found in serum calcium, alu?minum, arsenic, cobalt, magnesium, phosphorus, iron, parathyroid hormone and calcitonin concentration between the fam?ily members of patients and healthy controls (P>0.05). Family members from 8 FIBGC families including the two with consanguineous marriage manifested autosomal dominant heredity. The severity of , symptomatic s was correlated with VBGCpatients showed the same clinical manifestations in the dyskinesia family. The psychiatric symptoms was not asso? ciated with VBGC whereas patients with dyskinesia had a large VBGC. There was a significant difference in onset age be?tween patients with psychiatric symptoms and those with dyskinesia. P.atients with dyskinesia suffer larger VBGC, and is characterized by Patients with dyskinesia had relatively later onset age (43.95 ± 2.47 y) whereas those with. psychiatric symptoms hadsymptomatic patients with early onset age (31.32±10.16y). The comparison of the onset age (43.954±2.473 vs. 31.319±10.156 y, t=4.438, P=0.001) and VBGC (1.748±0.622 vs. 0.392±0.276 cm3, t=2.518, P=0.028) with symptom?atic patients between dyskinesia and psychogenic families was significant. Conclusions Eight FIBGC families manifested autosomal dominant heredity. Patients with dyskinesia suffer have a larger VBGC and are associated with a, and is char?acterized by relatively later onset age. In contrast, patients with psychiatric symptomspsychogeny is not related withhave a the small VBGC and showedand their age of onset is young. earlier onset age.

Objective To detect the global level of histone 4 lysine 20 (H4K20) methylation and its relation with DNA damage-repair in peripheral blood cell of arsenic-exposed residents in the coal-contaminated arsenism areas in Guizhou,in order to provide a basis to deepen the interpretation of the role of arsenic in inhibiting DNA damage-repair.Methods Jiaole village in coal-burning-borne arsenism areas in Xingren County of Guizhou was selected as the survey point,and 115 cases of arsenic-exposed residents were selected as the arsenic exposed group on the basis of physical examination.Moreover,53 residents from one village of non-epidemic area neighboring the diseased area were selected as controls.Hair and peripheral blood samples of these subjects were collected,and the histone protein was extracted from the lymphocytes separated from blood samples.The hair samples were digested with microwave digestion instrument,and the hair arsenic content was tested via the inductively coupled plasma-mass spectrometry (ICP-MS) method;the level of H4K20 1,2,3 methylation (H4K20me2,me2,me3) in peripheral blood cell was tested by enzyme-linked immunosorbent assay (ELISA);DNA damage of peripheral blood cell was measured by single cell gel electrophoresis (SCGE).Results The testing results of hair arsenic contents showed that the arsenic levels of hair in arsenic exposed group [0.30 (0.19-0.46)μg/g] were significantly higher than those of control group [0.12 (0.08-0.18) μg/g,F=11.968,P ＜ 0.05].Compared with the control (0.44 ± 0.14,0.99 ± 0.41,1.06 ± 0.33),the level of H4K20me1 (0.60 ± 0.29) in arsenic exposed group was higher (F =2.513,P ＜ 0.05),H4K20me2 (0.75 ± 0.26) was lower (F =4.707,P ＜ 0.05),and H4K20me3 (1.20 ± 0.62) was of no significant difference (F =0.582,P ＞ 0.05).The detecting results of DNA damage of the lymphocytes separated from peripheral blood showed a statistically significant increase (F =9.307,9.457,all P ＜ 0.05) in TailDNA％ and Olivetailmoment in arsenic exposed group [Median (M):10.75,11.69]compared with those of control group (M:2.12,1.16).The correlation analysis indicated that the arsenic levels of hair of subjects were positively correlated with H4K20me1 (r =0.214,P ＜ 0.05) and inversely with H4K20me2 (r =-0.224,P ＜ 0.05);H4K20me1 was positively associated with TailDNA％ (r =0.383,P ＜ 0.05) and Olivetailmoment (r =0.380,P ＜ 0.05);H4K20me2 was inversely associated with TailDNA％ (r =-0.290,P ＜ 0.05) and Olivetailmoment (r =-0.298,P ＜ 0.05).Conclusion H4K20 methylated modification makes a response to arsenic exposure of human body,the alteration of H4K20mel/me2 may participate in regulating DNA damage-repair induced by arsenic in vivo.