Intravitreal injection of antiangiogenic agents is widely used to treat retinal vascular disease.This therapy can induce regression of neovascular vessels; reduce intraocular inflammation and retinal vascular permeability,and control macular edema.However the action period of these agents is short,and thus this therapy need repeated injections which cause higher operation risk and cost.Retinal laser photocoagulation therapy can close retinal capillary non-perfusion area and neovascular vessels,reduce macular edema caused by vascular leakage.However,as its therapeutic effect is based on the destruction of the retinal tissues in the lesion area,this therapy need longer time to show its effects.When the disease is controlled by this method,it may already induce some structural irreversible damages to the retina,especially the macular.This is why the visual acuity is not satisfactory in some patients,even though the disease get controlled,macular edema gets disappeared and anatomical structure of retina get improved.Properly evaluating all the pros and cons of retinal photocoagulation and intravitreal injection of antiangiogenic agents,will allow us to explore a better way to combine these two therapies to treat retinal vascular diseases.

OBJECTIVE:To evaluate the importance of monitoring serum concentration of Sodium Valproate(VPA)in the treatment of epileptic children.METHODS:Serum VPA concentration was determined by Chemiluminescence Enzyme Immunoassay(CLEIA),and the correlation between serum drug concentration and the daily dosage(mg?kg-1?d-1),dosage form,individual differences,blood sampling time and the clinical efficacy was analyzed.RESULTS:The serum VPA concentration which was lower than 50 mg?L-1 was found in 35.45% of the cases,and that which was higher than 100 mg?L-1 was found in 8.99%.CONCLUSION:The serum VPA concentration which was not in the therapeutic window can be resulted from multiple factors,and the monitoring results of the serum concentration should be analyzed comprehensively and made good use of.

The ocular fundus changes and the damage of visual function were various at different stages of diabetic retinopathy (DR). To get hold of timing and different therapic method correctly of early diagnosis, whole body treatment, laser photocoagulation and vitreous-retina surgery and adopting targeted interventions could help patients receiving the most reasonable and effective treatment at different stages,both of them are keys to reduce the damage of visual function.

Objective:To study the relationship between the cell density and the cell proliferation phenotype. Methods: Plate clonality assays was used to measure the impact of cell density to cell clonality and cell cycle in BT325、786-0、293、C6 and NIH3T3 cell lines. Results: The clonality decreased when the cells grown to confluence in NIH3T3 and 7860 cell lines respectively.It seem need more cells to decrease the clonality in 293 cell line but there is no relationship between cell density and cell clonality in BT325 and C6 cell lines.Cell cycle analysis show that cell density have no effect on BT325 and C6 but on 786-0、293 and NIH3T3 cell lines. Conclusion: There might exist preventer or preventers,which is proportional to the number of cells,of immortal stem cell to expand.In addition,the rate of stem cell expansion is proportional to that of cell mitosis in immortal cell lines.

Objective To investigate the effect of different doses of rosuvastatin Calcium in the treatment of coronary heart disease complicated with hyperlipemia and its effect on blood lipid level.Methods 100 patients with coronary heart disease complicated with hyperlipemia were randomly divided into the three groups according to the number table methods,the patients in group A received rosuvastatin Calcium 5mg/d,group B was given 10mg/d, group C was given 20mg/d.A treatment course was 4 weeks,and continuous treatment lasted 3 courses.The effects after treatment and serum TC,TG,LDL-C and HDL-C levels before and after treatment of the three groups were compared,and the occurrence of adverse reactions in the three groups of patients was recorded.Results The effective rate of C group in the treatment of coronary heart disease was 87.9%,the effective rate in the treatment of hyperlipi-demia was 93.9%,which were significantly higher than those in group A and group B (χ2 =6.54,P<0.05 );The serum TC,TG,HDL-C and HDL-C levels of three groups after treatment were significantly improved compared with before treatment,and which in C group were improved better than the other two groups(F=5.45,P<0.05);There was no significant adverse reactions in the three groups during treatment.Conclusion Large dose rosuvastatin Calci-um has a significant therapeutic effect in treatment of coronary heart disease complicated with hyperlipemia,which can effectively regulate blood lipid levels,with no obvious adverse reaction and high security,which is worthy of clinical application.

Objective To investigate the correlation between mechanical ventilation (MV) initiation time during cardiopulmonary resuscitation (CPR) and the prognosis of patients with in-hospital cardiac arrest (IHCA) in emergency . Methods Retrospective analysis of clinical records of patients with IHCA in emergency from January 2011 to April 2016 treated in Zhangye People's Hospital of Hexi University was performed. Patients with restoration of spontaneous circulation (ROSC) and were on MV with aged over 18 years were divided into early treated group (≤ 10 minutes) and later treated group (> 10 minutes) according to the initiation time of MV. Corneal reflex, pupillary reflex, pain-avoidance responses and motor response 24 hours after CPR, neurological function and cure rate of the two groups were analyzed. Results 210 patients were selected into our study including 130 males and 80 females (mean age: 60.24±13.17 years). There were no significant differences in gender, age, type of heart rate and etiological factor of cardiac arrest (CA) between the MV early stage group (124 cases) and the MV late stage group (86 cases). The restoration of corneal reflex, pupillary reflex, pain-avoidance responses, motor response and achievement ratio of CPR in early group were higher than those of later group (respectively, 59.68% vs. 31.40%, 59.68% vs. 31.40%, 54.84% vs. 24.42%, 54.84% vs. 24.42%, 70.16% vs. 51.16%, all P < 0.01); The D-dimer levels in the early group patients were significantly lower than those in the later group (μg/L: 478.39±57.21 vs. 510.05±62.83, P < 0.01). However, no statistical difference was observed between the two groups with respect to pH (7.24±0.72 vs.7.13±0.67, P > 0.05); The average hospitalized day of the early group was significantly shorter than that of the later group (days: 24.15±3.04 vs. 30.28±4.17, P < 0.01); Besides, the early group showed a higher survival rate at discharge and had more cases with neurologic level of grade 1-2 than those of the later group (Respectively, 41.94% vs. 26.74%, P < 0.05; 44.35% vs. 15.12%, P < 0.01). Conclusion Initiation MV on IHCA patients in the early stage of CPR (≤ 10 minutes) could help improve the hypoxic condition and prognosis of neurological function, and increase the achievement ratio of CPR.

Adenine ; analogs & derivatives ; therapeutic use ; Animals ; Antiviral Agents ; therapeutic use ; Combined Modality Therapy ; Genetic Therapy ; Hepatitis B Antigens ; immunology ; Hepatitis B Vaccines ; therapeutic use ; Hepatitis B, Chronic ; therapy ; Humans ; Interferon-alpha ; administration & dosage ; Lamivudine ; therapeutic use ; Organophosphonates ; therapeutic use ; Recombinant Proteins

Humans ; Liver Failure

Anemia, Aplastic ; therapy ; Humans ; Treatment Outcome

Gene associated with retinoid-IFN-induced mortality-19(GRIM-19)is originally isolated as a growth suppressive gene using a genetic screen.GRIM-19, initially found in nucleus and mitochondria, is essential for the assembly and functioning of mitochondrial complex I.GRIM-19 involves the regulation of cell proliferation and apoptosis, and low expression or mutation of GRIM-19 contributes to abnormal proliferation.During viral oncogenesis, GRIM-19 may be a general target protein similar to other cellular tumor suppressors.GRIM-19 plays an important role in tumor formation and apoptosis inhibition, and may be as a new tumor marker to early cancer screening.