1.How to confirm it is motor neuron amyotrophy resulting from spinal anterior horn cells damage
Chinese Journal of Practical Internal Medicine 2001;0(02):-
To confirm a patient's muscular dystrophy is a motor neuron amyotrophy or not,we should take history in detail,physical examination completely,and assistant test reasonably.The history include the first onset symptom such as dysarthrosis,dysphagia,weakness and muscular dystrophy in the hand.We should pay attention to the fasciculation,insensibility,sphincter problem,the symptom progress,and family history.The lab tests include serum creating kinas,electromyogram,muscle biopsy,and gene(SOD1,SMA)analysis.It is important distinguish motor neuron amyotrophy from other muscular dystrophy with the cause of the spinal anterior horn cells damage clear.
2.Study on p16 methylation status of BEP-2D cells during its malignant transformation by cigarette smoke condensate(CSC)
Journal of Chongqing Medical University 2003;0(05):-
Objective:To study p16 methylation of BEP-2D cells during its malignant transformation.Methods:Normal BEP-2D cells and BEP-2D cells treated by cigarette smoke condensate(CSC) for 15 weeks(W-15),25 weeks(W-25),38 weeks(W-38) and 43 weeks(W-43)respectively were chosen to study the p16 methylation status by Nested methylation specific PCR (Nested-MSP).Results:The p16 methylation was found in W-15,W-25,W-38 and W-43 BEP-2D cells,but not in the normal BEP-2D cell.Conclusion:The p16 methylation may be considered as a kind of biology-marker of the early diagnosis of lung cancer because the p16 methylation occurs in the earlier period of lung cancer.
3.Clinical analysis of pemetrexed chemotherapy combined with radiotherapy in brain metastases of lung adenocarcinoma
Chinese Journal of Practical Nursing 2013;29(19):4-7
Objective To observe the clinical effects of pemetrexed chemotherapy combined with radiotherapy in brain metastases of lung adenocarcinoma patients.Methods The data of lung adenocarcinoma patients with brain metastases were conducted retrospectively diagnostic analysis,and they were stratified randomized divided into the observation group (26 cases) and the control group (25 cases).The observation group was treated with pemetrexed chemotherapy combined with radiotherapy; the control group was treated with radiotherapy alone.Brain metastases,quality of life,short-term efficacy,adverse reactions,and patients' survival period were observed.Results The efficacy and quality of life of patients in the observation group were improved significantly than those of the control group,and adverse reactions were mild,the survival period was not significant between two groups.Conclusions Pemetrexed in combination with radiation therapy for the treatment of brain metastases from lung adenocarcinoma can effectively improve the short-term efficacy and quality of life of patients,and the adverse reactions are mild,it possess great application value in the treatment of brain metastases of lung adenocarcinoma.
4.How to diagnose muscular atrophy in children
Journal of Clinical Pediatrics 2009;(11):1007-1013
In clinical practice, muscular atrophy is a common sign in children. Because of relatively thick subcutaneous fat in children, muscular atrophy is not easy to be discovered. In order to confirm the diagnosis earlier, it is very important to take history in detail, to observe the motor function, cry, the posture of sitting and standing carefully, to do the physical examination thoroughly, and to use the assistant test reasonably.
5.Study on the prevention and treatment of multiple organ dysfunction syndrome following cardiac surgery
Journal of Chongqing Medical University 2003;0(05):-
0.05);There are significant differences between treatment group and control group in moderate and severe patients(P
6.Pharmacokinetics of Nalmefene hydrochloride in healthy volunteers
Journal of Chongqing Medical University 2007;0(09):-
Objective:To study the phamacokinetics of Nalmefene hydrochloride. Methods:30 healthy Chinese volunteers were assigned to 3 groups according to randomized design, and each group were given respectively a single intravenous administration dose of 0.5 mg, 1mg and 2mg of Nalmefene hydrochloride. The electrospray ionization positive selected reaction monitoring detections for Nalmefene hydrochloride and allyln-oroxymorphone (the internal standard) in the plasma and in the urine were used. The pharmaceutical parameters were calculated by 3P87 software. Results: The limit of the detection for Nalmefene hydrochloride was 0.0008 ng/ml in plasma and 0.0003 ng/ml in urine. There were good linear relationship range from 0.04 to 625ng/ml in plasma and 0.2 to 625 ng/ml in the urine. The variation coefficient in day and days, and the recoveries fitted the clinical pharmacokinetic study requirements. After a single intravenous administration of 0.5 mg,1mg, and 2mg of Na-lmefene hydrochloride, the obtained pharmacokinetic parameters were respectively as follows:Cmax were(1.74?0.48)?g/L,(4.17?1.08) ?g/L,(7.88?2.89) ?g/L;t1/2? were(12.83?3.98) h,(12.6?6.4) h, (10.58?3.74) h;V were (3.45?0.16) L/kg;(4.11?0.0716)L/kg、(4.25?0.56)L/kg;CL were (0.81?0.11) L/(h?kg), (0.85?0.15)L/(h?kg), (0.87?0.11) L/(h?kg);AUC0-∞ were (21.62?9.34) (ng/L)*h,(24.89?8.52) (ng/L)*h, (35?11.2) ng/L)*h;the urine accumulatived exc-retory rate were (78?21)%,(71?29)%,(78?19)%. Conclusion: The determination method for Nalmefene hydro-chloride in the plasma and in the urine by the electrosprayionization positive selected reaction monitoring detections is exclusive, accurate, sensitive and suitable. Pharmacokinetic characteristics in 3 groups of human treated with intravenous administration were described by a two-compartment model. The pharmaceutical characteristics of doxycycline hydrochloride in human body were linear in the range of 0.5~2.0 mg.
7.Clinical efficacy of laparoscopic diaphragmatic hernia repair
Maisiyiti ALIMUJIANG ; Cheng ZHANG
Chinese Journal of Digestive Surgery 2015;14(10):832-834
Objective To investigate the clinical efficacy of laparoscopic diaphragmatic hernia repair.Methods The clinical data of 15 patients who underwent laparoscopic repair for acquired diaphragmatic hernia at the People's Hospital of Xinjiang Uyghur Autonomous Region from August 2011 to June 2015 were retrospectively analyzed.Two-side composite patch at the hernia ring-center was fixed on the diaphragm using laparoscopic hernial screw nail for defect repair.The follow-up was performed by telephone interview and outpatient examination till August 2015, which included postoperative recurrence of hernia and complications.Measurment data with normal distribution were presented as mean value (range).Results Fifteen patients received successful laparoscopic repair for tension-free diaphragmatic hernia.The operation time, volume of intraoperative blood loss and average duration of hospital stay were 134.5 minutes (range, 120.0-150.0 minutes), 25.6 mL (range, 10.0-50.0 mL) and 5 days (range, 10-12 days), respectively.No postoperative complications of respiratory and digestive system in 15 patients were detected, and there were the remissions of preoperative respiratory symptoms (cough, cough sputum, wheezing and suffocating) and digestive symptoms (nausea, vomiting and acataposis) of some patients.All the 15 patients were followed up for 2-48 months without severe postoperative complications and recurrence.Conclusion Laparoscopic diaphragmatic hernia repair is safe and feasible.
8.Progress of t (14;18) transiocation and c-myc gene rearrangement in diffuse large B-cell lymphoma
Hongwei ZHANG ; Niuliang CHENG
Journal of Leukemia & Lymphoma 2009;18(10):630-633
Diffuse large B-cell lymphoma (DLBCL) is a heterogeneous group of tumors composed of large B cells. It is the most common type of non-Hodgkin lymphoma. As its variety of clinical, morphological and genetic characters, the classification and prognosis are still debated. This article aims to elucidate t(14;18) translocation and c-myc gene rearrangement is of great significance to the clinical diagnosis, classification, treatment and prognosis of DLBCL.
9.Serum progesterone levels in early pregnant women and its relation to luteal maintenance therapy for pregnancy protection
Chinese Journal of General Practitioners 2015;14(4):269-273
Objective To investigate serum levels of β-human chorionic gonadotrophin (β-HCG) and progesterone (P) in early pregnant women,and their relation to luteal maintenance therapy for early pregnancy protection.Methods One hundred and thirty five infertility women treated in Department of Gynecology and Obstetrics of Beijing Chaoyang Hospital from January 2007 to December 2011.Total 150pregnancy cycles,including 84 with intrauterine insemination (IUI) and 66 with natural conception,were divided into two groups:normal intrauterine pregnancy group (group A,n =80) and early pregnancy loss group (group B,n =70).Medical history,ultrasonic findings,serum female hormone,P and β-HCG levels at early pregnancy stage were analyzed.Results The age of group A and group B was (30.0 ± 3.9) years and(30.7 ± 4.9) years,respectively (P > 0.05).The follicle-stimulating hormone/luteinizing hormone in group A and group B was 1.57 ±0.96 and 1.56 ± 1.08 ; the estradiol levels in two groups were (152 ±66) pmol/L and (147 ± 69) pmol/L,respectively (both P > 0.05).There were no differences in dominant follicles and endometrial thickness between groups A and B (P > 0.05).Ovulation promotion and luteal support treatments were adopted in both groups:50% (40/80) of cycles in group A received ovulation promotion,73% (58/80) of cycles received luteal support,while 44% (31/70) and 76% (53/70) received in groups B,respectively.The levels of serum progesterone in group A during 14-21 d,22-27 d and ≥ 28 d after ovulation were higher than those in group B at each time points (P < 0.0l).There were no significant differences in serum progesterone levels between women with luteal support treatment and those without luteal support treatment in both groups (P > 0.05).Conclusion Dynamic monitoring of serum progesterone level in early pregnancy may be used as an auxiliary index for prediction of pregnancy outcome,but it may not be an indicator for luteal maintenance therapy.
10.Clinical application of basilic vein transposition arteriovenous fistula in hemodialysis patients
Clinical Medicine of China 2015;31(6):535-538
Objective To investigate the clinical effect of basilic vein transposition arteriovenous fistula in hemodialysis patients.Methods NInety patients with maintenance hemodialysis received in the NO.180 Hospital of the Chinese People's Liberation Army from September 2011 to September 2012 were randomly divided into two groups,and each group of 45 cases.Patients in the observation were given brachial-basilica transposition arteriovenous fistula,while patients in the control group received artificial vascular graft arteriovenous fistula.The fistula maturation time,dialysis blood flow,urea removal index (Kt/V),patency rates and complications were respectively recorded.Results Compared with control group,fistula maturation time,dialysis blood flow,Kt/V and complications rates in observation group were significantly higher((14.4±3.2)weeks vs.(16.1±2.7) weeks,(291.5±33.9) ml/min vs.(252.6±29.8) ml/min,(1.6±0.2) vs.(1.3±0.3);t =4.538,3.984,4.016;P< 0.05).Complications (ipsilateral upper limb swelling,thrombosis,venous ectasia and arteriovenous fistula stenosis) incidence were significantly lower than those of control group (2.2%vs.13.3%,2.2% vs.11.1%,6.6% vs.17.8%,11.1% vs.24.4%;x2=5.463,4.972,5.017,3.968;P <0.05).Patency rates of observation group in 3 months,6 months,1 year and 2 years were also significantly higher than those of control group (97.8% vs.93.3%,91.1% vs.84.4%,88.9% vs.75.6%,84.4% vs.68.9%,x2 =5.315,4.238,7.024,5.913;P<0.05).Conclusion Basilic vein transposition arteriovenous fistula is reliable and effective for hemodialysis patients.It can achieve adequate dialysis and less complications and It is worth of clinical application.