Venous thrombotic disease is a serious disease,which impact on health and life-threatening.Pulmonary embolism and deep-vein thrombosis are the two components of a single disease called venous thromboembolism in obstetrics and gynecology.but it can be difficult to diagnose because clinical symptoms and signs are non-specific or absent in early venous thrombus embolism (VTE).It has great value that how to use the most economic,simple,efficient method for screened high-risk groups,timely and accurate laboratory diagnosis of VTE.

Objective:To study the surface enhanced Raman scattering ( SERS) of safflower and identify safflower injections by SERS quickly and effectively. Methods:Through comparative analysis of the Raman spectroscopy of safflower injections and the corre-sponding control herbs, the rapid identification of safflower injections was realized. Results:The results showed that several character-istic peaks of safflower were enhanced obviously in SERS, which could be used to identify safflower injections. Conclusion:The meth-od is reliable, rapid, accurate and specific, which can be applied as a method to identify safflower and its injections.

ObjectiveTo develop the military acute stress scale and examine the reliability and validity of this scale.MethodsBased upon the previous acute stress researches and the rule of psychometrics,the military acute stress scale was compiled according to open-ended inventory survey.A total of 630 personnel were chosen by random cluster sampling,and tested with this scale,50 of them were tested by Chinese Military psychosomatic health Scale simultaneously,and 50 of them were retested after two week.The reliability and validity were examined by exploratory factor analysis,confirmatory factor analysis and correlation analysis.ResultsThe military acute stress scale was comprised of 9 factors and one lie sub-scale ; Main fit indexes of model were the following CMIN =1219.476,DF =593,P =0.000,CMIN/DF =2.056,IFI =0.927,TLI =0.902,CFI =0.924,RMSEA =0.059; The conrelation coefficients between subscales and subscales were 0.284-0.572.The correlation coefficients between subscales and total scale were 0.700 ～ 0.846 (P ＜ 0.05 or 0.01 ) ; The correlation coefficients was significant between this scale and Chinese military psychosomatic health scale.The correlation coefficients were 0.338-0.962 (P ＜ 0.01 ).The test-retest correlation coefficients of total scale and subscales were 0.379-0.909 (P ＜ 0.01 ).The Cronbaeh's α coefficients were 0.606-0.926 ; and the split-half correlation coefficients were 0.466-0.855.ConclusionThe military acute stress scale has acceptable psychometrics properties on reliability and validity.

Objective To detect the effects of chemotherapy on the immune function of patients with ovarian cancer.Methods Thirty-four patients with ovarian cancer(ovarian cancer group)who accepted chemotherapy were collected.Thirty healthy women were used as control group.The lymphocyte sub-cluster CD3+,CD4+,CD8+ and CD25+of peripheral blood were detected by flow cytometry.Results The peripheral blood CD3+,CD4+ and CD4+/CD8+ in ovarian cancer group were lower than those in control group(0.5524±0.1121 vs.0.6685±0.0869,0.3183±0.0864 vs.0.4139±0.0524,1.06±0.29 vs.1.43±0.21),CD4+ CD25+was higher than that in control group(0.1026±0.0418 vs.0.0287±0.0132),there was significant difference between two groups(P<0.05).There was no significant changes observed 1 week after chemotherapy(P>0.05).Three weeks after chemotherapy,the CD3+,CD4+ and CD4+/CD8+ in ovarian cancer group were higher and CD4+ CD25+ was lower,there were significant difference between two groups(P<0.05).Conclusions The ratio of lymphocyte sub-cluster is imbalance in the patients with ovarian cancer.Chemotherapy can improve the immunosuppression condition and recovery the ratio of lymphocyte sub-cluster in the patients with ovarian cancer.

Based on the analysis of the medical students' humanities quality and current college English teaching,this paper explored four strategies including pre-class lead-in activity,making full use of current teaching materials,reforming present college English teaching method and developing extracur-ricular practical activities in order to foster humanities quality in college English teaching and to improve medical students' humanities quality.

Necrotizing enterocolitis(NEC) is a common and devastating gastrointestinal disease that occurs predominantly in premature infants.Despite various advances in management,the mortality of this disease remains high.During the last decade,studies have shown that heparin-binding epidermal growth factor-like growth factor (HB-EGF),can protect intestinal epithelial cells from various forms of injury in vitro.Furthermore,studies using both an intestinal ischemia-reperfusion injury model in adult rats and neonatal rat model of NEC,have shown that HB-EGF can protect the intestines from injury.Present studies provide a new research direction in the prevention and treatment.

Objective To observe the effect and safety of different doses of sufentanil plus subarachnoid block during labor. Methods One hundred parturients (single birth) were divided into 5groups by random digits table with 20 cases in each, which sufentanil 3,4,5,6,7 μ g in group A, B, C, D, E plus ropivacaine 3 mg were injected respectively into subarachnoid space. The onset and duration of subarachnoid analgesia were observed; visual analogue scale (VAS) were recorded respectively before injection and 5,15,30,60 min after injection; the vital sign,labor stage,labor type,adverse reactions of parturients,fetal heart rate (FHR) and the Apgar score at 1,5 min of neonate were recorded respectively.Results The onset of subarachnoid analgesia in group A was higher than that in group B,C, D,E;the duration of subarachnoid analgesia in group A,B was shorter than that in group C,D,E.VAS declined gradually 5,15,30,60 min after injection compared with before injection in 5 groups (P ＜ 0.01 );VAS in group B, C, D, E was lower than that in group A at 5 min after injection (P ＜ 0.05 ), and VAS in group C, D, E was lower than that in group A,B at 60 min after injection (P＜ 0.05). There was no significant difference in vital sign,labor stage,labor type,FHR and the Apgar score of neonate among 5 groups (P ＞0.05). The occurrence of itch of skin and nausea in group D,E [25%(5/20), 15%(3/20) in group D,and 40%(8/20),20%(4/20) in group E] was higher than that in group A, B, C [5%(1/20), 0 in group A, 10%(2/20), 0 in group B, 10% (2/20), 0 in group C] (P ＜ 0.05 ). Conclusion Subarachnoid block (sufentanil 4-6 μ g +ropivacaine 3 mg) can offer safety and efficacy of labor analgesia; sufentanil 5 μg is the best dose with subarachnoid block during labor at present.

Objective To investigate the effect of Fructus Ligustri Lucidi(FLL),a kidney-tonifying Chinese herbal medicine,on calcium metabolism and vitamin D-dependent gene expression in ovariectomized(OVX) rats.Methods Four weeks after OVX surgical operation the OVX rats were ig admini-(stered) with estradiol and FLL for 14 weeks.Ca~(2+) Level in serum,urine,and feces was measured by colorimetric methods,and gene expressions in duodenum and kidney were analyzed by reversed transcriptase-polymerase chain reaction(RT-PCR).Results Ovariectomy of rat led to significant loss of calcium in vivo,as demonstrated by decreasing serum Ca~(2+) level and increasing Ca~(2+) excretion rate in urine and feces in OVX rat group.Treatment of OVX rats with FLL could effectively restore serum Ca~(2+) level and prevent the increase of Ca~(2+) excretion from urine and feces.RT-PCR Analysis showed that both estradiol and FLL could prevent OVX-induced decrease of mRNA expression in duodenal vitamin D receptor(VDR).In kidney,FLL did not alter mRNA expressions of 25-hydroxyvitamin D 1-hydroxylase(1-OHase) and 25-hydroxyvitamin D 24-hydroxylase(24-OHase),while increased the mRNA expression levels of renal cal-ciumbinding protein-9k(CaBP-9k) and calcium-binding protein-28k(CaBP-28k).Conclusion FLL could improve the state of calcium imbalance induced by estrogen deficiency and the potential mechanism that contributes to the improvement of calcium status by FLL might involve the increase in intestinal sensitivity to active vitamin D through its up-regulation of VDR expression in duodenum and the enhancement in Ca~(2+) reabsorption via the up-regulation of calcium binding proteins expression in kidney.

Objective:To investigate the expression of five drug resistance genes in hepato cellular cancer,lung cancer,stomach cancer,colorectal cancer,ovarian cancer,breast cancer tissue. Methods:The expression of P-gp,MRP,LRP,GST-?,Topo-Ⅱ in 120 cases of malignant tumors were examined by immunohistochemistry stain. Results: 1. The expression of P-gp,MRP,LRP,GST-? in the 6 common cancers were relatively high,while Topo-Ⅱ was relatively low. 2. P-gp,MRP,LRP,GST-?,Topo-Ⅱ were all high expressed in hepatocellular cancer. 3. The coexpression rate of two and more genes was 95.83%,the coexpression rate of five genes was 21.67%. Conclusion:P-gp,MRP,LRP,GST-?,Topo-Ⅱ are associated with multidrug resistance in different degree,which can be regarded as valuable indicators for the decision of individual chemotherapeutic program.

Objective:To identify the best method for isolating and culturing mature rat adipocytes in vitro.Methods:Three different primary culture methods,i.e.organ culture,ceiling culture,and modified ceiling culture were used in the present study.Mature Sprague-Dawley rat adipocytes were cultured in vitro.The cells were observed under microscope and oil red O staining to compare the distinction of cells with different culture methods in the same condition.Rusults:Adipocytes were successfully cultured by the three primary cell culture methods.Cells cultured were highly homogenous.Modified ceiling culture method was the best in terms of the efficiency of culturing and cell morphology.Conclusion:Modified ceiling culture,is a long-term stable primary culture method for mature rat adipocyte,providing a good technique platform for the studies related to adipocytes.