Objective:To study the surface enhanced Raman scattering ( SERS) of safflower and identify safflower injections by SERS quickly and effectively. Methods:Through comparative analysis of the Raman spectroscopy of safflower injections and the corre-sponding control herbs, the rapid identification of safflower injections was realized. Results:The results showed that several character-istic peaks of safflower were enhanced obviously in SERS, which could be used to identify safflower injections. Conclusion:The meth-od is reliable, rapid, accurate and specific, which can be applied as a method to identify safflower and its injections.

Venous thrombotic disease is a serious disease,which impact on health and life-threatening.Pulmonary embolism and deep-vein thrombosis are the two components of a single disease called venous thromboembolism in obstetrics and gynecology.but it can be difficult to diagnose because clinical symptoms and signs are non-specific or absent in early venous thrombus embolism (VTE).It has great value that how to use the most economic,simple,efficient method for screened high-risk groups,timely and accurate laboratory diagnosis of VTE.

ObjectiveTo develop the military acute stress scale and examine the reliability and validity of this scale.MethodsBased upon the previous acute stress researches and the rule of psychometrics,the military acute stress scale was compiled according to open-ended inventory survey.A total of 630 personnel were chosen by random cluster sampling,and tested with this scale,50 of them were tested by Chinese Military psychosomatic health Scale simultaneously,and 50 of them were retested after two week.The reliability and validity were examined by exploratory factor analysis,confirmatory factor analysis and correlation analysis.ResultsThe military acute stress scale was comprised of 9 factors and one lie sub-scale ; Main fit indexes of model were the following CMIN =1219.476,DF =593,P =0.000,CMIN/DF =2.056,IFI =0.927,TLI =0.902,CFI =0.924,RMSEA =0.059; The conrelation coefficients between subscales and subscales were 0.284-0.572.The correlation coefficients between subscales and total scale were 0.700 ～ 0.846 (P ＜ 0.05 or 0.01 ) ; The correlation coefficients was significant between this scale and Chinese military psychosomatic health scale.The correlation coefficients were 0.338-0.962 (P ＜ 0.01 ).The test-retest correlation coefficients of total scale and subscales were 0.379-0.909 (P ＜ 0.01 ).The Cronbaeh's α coefficients were 0.606-0.926 ; and the split-half correlation coefficients were 0.466-0.855.ConclusionThe military acute stress scale has acceptable psychometrics properties on reliability and validity.

Objective:To explore the opportunity and the manner of termination of pregnancy.Methods:The data of 200 cases of ICPfrom January 2007 to June 2008 were retrospectively analyzed.Maternal pruritis,liver function ,glycocholic acid(CG),amniotic fluid condition, the time and the way of termination of pregnancy,Apgar score were examined.Three groups were classified by the values of CG:Group A (CG

OBJECTIVE:To evaluate the efficacy of pulmonary surfactan (PS) in the treatment of acute respiratory distress syndrome (ARDS) in infants after undergoing cardio-pulmonary bypass. METHODS: Twenty ADRS infants were randomly assigned to receive routine therapy alone (control group) or routine therapy in combination with PS (trial group). The clinical efficacy in two groups was monitored. RESULTS: In the trial group compared with the control group, the mortality rate was significantly lower(P

Necrotizing enterocolitis(NEC) is a common and devastating gastrointestinal disease that occurs predominantly in premature infants.Despite various advances in management,the mortality of this disease remains high.During the last decade,studies have shown that heparin-binding epidermal growth factor-like growth factor (HB-EGF),can protect intestinal epithelial cells from various forms of injury in vitro.Furthermore,studies using both an intestinal ischemia-reperfusion injury model in adult rats and neonatal rat model of NEC,have shown that HB-EGF can protect the intestines from injury.Present studies provide a new research direction in the prevention and treatment.

Objective:To observe the effect of intrapulpal injection in the pulpectomy of primary molars.Methods:22 petients with 44 diseased primary molars were divided into 2 groups(n =22 teeth).The intrapulpal injection with a computer-controlled local anesthetic delivery system STATM was performed in the experimental Group,and traditional infiltration anesthesia technique was conducted in the control group.Results:During the anesthesia process,the VAS value of the experimental group was lower than the control group(P ＜ 0.05).The pain control in the experimental group was better during pulp removal within 1 min.There was no occurrence of postoperative soft tissue numbness and factitial ulcer in the experinental group.Conclusion:The intrapulpal injection,administered with STATM,is more effective than traditional infiltration anesthesia in pulpectomy of primary molar teeth.

Objective: To establish an HPLC method for the determination of gentiopicroside,gardenia and baicalin in Xiegan Anshen pills.Methods: The analysis was carried out on a Phenomenex Luna C18(2) column(250 mm×60 mm, 5 μm).The mobile phase consisted of methanol-0.2% phosphoric acid solution with gradient elution and the flow rate was 1.0 ml·min-1.The column temperature was 30℃, the detection wavelength was 254nm, and the sample size was 10 μl.Results: The linear range of gentiopicroside was 10.55-168.80 μg·ml-1 (r=0.999 9),and the average recovery was 98.68%(RSD=1.2%, n=6).The linear range of gardenia was 29.85-477.60 μg·ml-1 (r=0.999 8), and the average recovery was 98.76%(RSD=1.1%, n=6).The linear range of baicalin was 43.05-688.8 μg·ml-1 (r=0.999 7), and the average recovery was 98.36%(RSD=1.4%, n=6).Conclusion: The method is simple and accurate, and can determine the contents of the three ingredients simultaneously, which can be used for the quality control of Xiegan Anshen pills.

Objective To observe the effect and safety of different doses of sufentanil plus subarachnoid block during labor. Methods One hundred parturients (single birth) were divided into 5groups by random digits table with 20 cases in each, which sufentanil 3,4,5,6,7 μ g in group A, B, C, D, E plus ropivacaine 3 mg were injected respectively into subarachnoid space. The onset and duration of subarachnoid analgesia were observed; visual analogue scale (VAS) were recorded respectively before injection and 5,15,30,60 min after injection; the vital sign,labor stage,labor type,adverse reactions of parturients,fetal heart rate (FHR) and the Apgar score at 1,5 min of neonate were recorded respectively.Results The onset of subarachnoid analgesia in group A was higher than that in group B,C, D,E;the duration of subarachnoid analgesia in group A,B was shorter than that in group C,D,E.VAS declined gradually 5,15,30,60 min after injection compared with before injection in 5 groups (P ＜ 0.01 );VAS in group B, C, D, E was lower than that in group A at 5 min after injection (P ＜ 0.05 ), and VAS in group C, D, E was lower than that in group A,B at 60 min after injection (P＜ 0.05). There was no significant difference in vital sign,labor stage,labor type,FHR and the Apgar score of neonate among 5 groups (P ＞0.05). The occurrence of itch of skin and nausea in group D,E [25%(5/20), 15%(3/20) in group D,and 40%(8/20),20%(4/20) in group E] was higher than that in group A, B, C [5%(1/20), 0 in group A, 10%(2/20), 0 in group B, 10% (2/20), 0 in group C] (P ＜ 0.05 ). Conclusion Subarachnoid block (sufentanil 4-6 μ g +ropivacaine 3 mg) can offer safety and efficacy of labor analgesia; sufentanil 5 μg is the best dose with subarachnoid block during labor at present.

Objective To investigate the dose-effect relationship of sufentanil combined with dexmedetomidine which used in clinical effectiveness and safety of intravenous analgesia after hip arthroplasty.Methods Sixty patients (after hip arthroplasty) were randomly divided into three groups:group A (sufentanil 0.5 μg/kg),group B (sufentanil 0.75 μg/kg),and group C (sufentanil 1 μg/kg).Each group was combined with dexmedetomidine 1.5 μg/kg in patient controlled analgesia (PCA) pump.Mean arterial pressure (MAP),heart rate (HR),SpO2,numerical rating scale (NRS) scores,and Ramsay sedation scores were recorded at T1 (4 h after operation),T2 (8 h after operation),T3 (24 h after operation),and T4(48 h after operation).Harris hip scores were recorded at T0(preoperative),T3,and T4.The total number of pushing patient controlled intravenous analgesia (PCIA),dezocine dosage,and the occurrence of adverse events in each group situation were recorded.Results There were no significant differences in MAP,HR,and SpO2 among groups.Compared to group A,NRS scores of groups B and C were lower during the analgesia (P ＜0.01).Compared to group A,Ramsay sedation scores of groups B and C were higher at T1,and T2 (P ＜ 0.01).Compared to group A,Harris hip scores of groups B and C were higher at T3,and T4 (P ＜ 0.01).Compared to group C,adverse reactions of groups A and B were less during the analgesia (P ＜ 0.01).Compared to group A,the total number of press PCIA and dezocine amount were decreased in groups B and C (P ＜ 0.01).Conclusions The 0.75 μg/kg sufentanil combined with 1.5 μg/kg right beauty holds the minimum for intravenous analgesia effect after hip arthroplasty and calm exactly satisfied,less adverse reaction,for PCIA ideal drug formula.