1.ISOLATION OF A DEEP-SEA STRAIN THAT PRODUCES ALKALINE PROTEASE AND STUDIES ON ITS FERMENTATION CONDITION
Microbiology 1992;0(04):-
From 38 pieces of deep\|sea samples,a strain,DY\|A,which produces protease was isolated.It can grow at a wide range of pH and salt concentration and best at 10℃.The strain produced maximum protease activity after growth at 10℃,initial pH 10 0 and inoculation volume 0 5%for 48~72h on a shaker by speed of 200r/min.The optimum pH and temperature for the protease activity production are pH 10.0 and 40℃.The enzyme is alkaline protease.Some other properties of the strain and the protease were discussed in detail.
2.Effective penetration of cell-permeable peptide mimic of tyrosine residue 654 domain of beta-catenin into human renal tubular epithelial cells.
Rui, ZENG ; Gang, XU ; Min, HAN
Journal of Huazhong University of Science and Technology (Medical Sciences) 2007;27(6):630-4
Phosphorylation of beta-catenin tyrosine residue 654 plays an important role in the epithelial to myofibroblast transition (EMT). Introducing mimic peptide of tyrosine residue 654 domain of beta-catenin into cells may influence phosphorylation of beta-catenin tyrosine residue 654. To deliver this mimic peptide into renal epithelial cells, we used penetratin as a vector, which is a novel cell permeable peptide, to deliver hydrophilic molecules into cells. A tyrosine 654 residue domain mimic peptide of beta-catenin (PM) with fused penetratin was constructed, purified and then detected for the penetration of the mimic peptide into human renal tubular epithelial cells (HK-2). The results showed that purified fusion mimic peptide could efficiently and rapidly translocate into human renal tubular epithelial cells. It is concluded that a cell-permeable peptides mimic of tyrosine residue 654 domain of beta-catenin was successfully obtained, which may provide a useful reagent for interfering the human renal tubular epithelial-mesenchymal transition.
Carrier Proteins/*metabolism
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Epithelial Cells/cytology
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Epithelial Cells/*metabolism
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Fibroblasts/cytology
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Fibroblasts/metabolism
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Kidney Tubules/*cytology
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Peptides/metabolism
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Permeability
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Phosphorylation
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Tyrosine/*metabolism
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beta Catenin/*metabolism
3.Karyotype analysis of second-trimester amniotic fluid cells in 572 high-risk pregnant women
Rui ZHU ; Aiqun ZENG ; Jingchun DU
The Journal of Practical Medicine 2016;32(18):3050-3052
Objective To explore the relationship between prenatal diagnosis indications and fetal chromosomal aberrations , and the security of amniocentesis. Methods The amniotic fluid cells were sampled by amniocentesis and cultured in 572 high-risk pregnant women from January 2012 to August 2015. The chromosomal karyotypes were examined by G-banding. Results The success rate of the first amniotic fluid cells culture reached 99.83%. In all the 572 valid samples , there were 20 cases of chromosomal aberrations and the abnormal rate was 3.50%, including 17 of numeric aberrations and 3 of structural aberrations. There were 7 cases of chromosomal aberrations in all the 299 elderly parturient in high-risk indications and the abnormal rate was 2.34%, and there were 13 cases of chromosomal aberrations in all the 273 non-elderly parturient and the abnormal rate was 4.76%. Conclusions (1)It is necessary to further diagnose in pregnant women with high-risk factors , including high age , abnormal screening and ultrasonic findings , and history of abnormal gestation and birth. (2)The chromosomal karyotype examination of amniotic fluid cells in high-risk pregnant women is one of the effective prenatal diagnosis methods in high security and accuracy , with which it can reduce the incidence of birth defects and the burden of family and society , and improve the quality of the population.
4.Research on Enzyme Linked Immunosorbent Assay for Erythropoietin
Zhaowei ZENG ; Rui WANG ; Hui SUN
Tianjin Medical Journal 2009;37(12):1029-1031
Objective: To establish an enzyme linked immunosorbent assay(ELISA)for erythropoietin(EPO) in serum, and observe its clinical application value thereof. Methods: Prepare the EPO polyclonal antibody, wash the plate with isopropyl alcohol, and then choose the suitable concentration of the antibody, enzyme labeled antibody, and antigen. After the reaction, check the sensitivity, recovery, specificity and stability of the method. The serum samples of anaemia and breast carcinoma individuals were detected. The results of radioimmunodetection were compared with that of normal control group. Results: The immo-assay plate showed strong adherence to proteins. The optimal concentrations of the antibody, enzyme labelled antibody and antigen were 1∶1 000, 1∶6 000 and 1∶800 separately. The sensitivity was 0.46 U/L. The cross-reaction with growth hormone and ferritin was low. The mean recoveries of samples with high and low concentrations were 96.3%, 97.3% respectively. The coefficients of variation of intra-assay and inter-assay were just 8.31% and 7.82%, and the stability was good. The EPO levels were higher in anaemia and breast carcinoma groups than that of normal group. There was no significant difference between the results of the radioimmunodetection and ELISA. Conclusion: The double-antibody sandwich ELISA method was established for EPO in serum, which had certain clinical application value.
5.Patient radiation dose of spine X-ray panoramic imaging: Comparison of two techniques based on Monte Carlo mathematical model
Rui JIN ; Yongming ZENG ; Xianfan LIU
Chinese Journal of Radiological Medicine and Protection 2015;35(10):792-796
Objective To compare patient radiation dose of two techniques of spine X-ray panoramic imaging under the same image quality.Methods An anthropomorphic phantom was used.The reasonable imaging parameters were found for spine panoramic radiography using Sonialvision safire17 Slot scan with HQ mode.And the panoramic films were obtained with different parameters using GE XR650 DR system.The panoramic images were scored by three experienced radiologists.The imaging parameters with the same score in groups were input into PCXMC 2.0 software to get effective dose(E) and organ dose.Results The reasonable imaging parameters of Slot scan were high quality(HQ) mode, SID 150 cm, 100 kVp, and 2 mAs;and the corresponding parameters of XR650 were SID 200 cm, 100 kVp, and 3.2 mAs.The E of the Slot scan with HQ mode, XR650 with manual mode and XR650 with AEC mode was(0.118 7±0.001 4),(0.0847±0.0008), and (0.1580±0.001 5) mSvrespectively, while the E of XR650 with manual mode was lower than the others(F =3 007.293, P <0.05).The organ dose using XR650 DR with manual mode were lower than that using Slot scan with HQ mode in all samples except breasts(P <0.05);the organ dose using XR650 DR with AEC mode were higher than that using XR650 DR with manual mode and Slot scan with HQ mode for all samples except for thyroid, oesophagus and lungs (P <0.05).Conclusions The radiation doses of both spine X-ray panoramic imaging with manual mode are low, and low dose spine X-ray panoramic imaging can be achieved if reasonable parameters are used.
6.Correlation Analysis between Quality ofRheum palmatumL. and Ecological Factors
Wenlong WEI ; Rui ZENG ; Linfang HUANG
World Science and Technology-Modernization of Traditional Chinese Medicine 2015;(9):1849-1854
This study was aimed to investigate the relationship between the quality ofRheum palmatum L. and ecological factors, in order to provide the basis for nurture and cultivation ofR. palmatumL. The ultra performance liquid chromatography (UPLC) was used in the determination of 8 effective components in samples from 3 majorR. palmatumL. producing provinces, which wereGansu,SichuanandQinghai province. The SPSS software was applied to analyze the effective components by the one-way analysis of variance. And the correlation analysis between the content and ecological factors was also conducted. The results showed that the pressure, relative humidity and temperature had close relationship with the content of bioactive compounds inR. palmatumL. The content of effective components inR. palmatumL. was positively related to pressure, relative humidity, and temperature. It was concluded that the quality ofR. palmatumL. in Gansu province was better. And the ecological factors affected the accumulation of effective components. This research provided experimental basis for the quality ofR. palmatumL. in different regions and ecological adaptation. The results demonstrated critical meaning forR. palmatumL. quality improvement, appropriate ecological division, and industrialized development promotion ofR. palmatumL.
7.Less invasive stabilization system for high-energy fractures of proximal tibia
Congfeng LUO ; Rui JIANG ; Bingfang ZENG
Chinese Journal of Orthopaedic Trauma 2004;0(12):-
Objective To report the preliminary results of Less Invasive Stabilization System (LISS) applied in treatment of proximal tibial fractures. Methods 22 patients with acute fractures of proximal tibia were treated with LISS plates from November, 2003 to June, 2004. There were 6 females and 16 males, with a mean age of 41.8 years (ranging from 22 to 69 years). According to AO classification, 7 cases were type 41- A, and 15 cases were type 41- C (9 combined with other fractures). 5 of these were open fractures (3 cases of Gustilo- Anderson type Ⅲ A and 2 cases of type Ⅲ B). Results All fractures were eventually healed. There were 2 cases of superficial infection. No deep infection, failure of fixation or malalignment was found in this group. However, 1 case showed displacement of articular surface 6 months postoperatively. The range of motion was from 2? to 98.6? . The mean time of radiographic healing was 14.5 weeks (ranging from 11 to 17 weeks) and the mean time of full weight bearing was 15.2 weeks (ranging from 12 to 18 weeks). By Johner - Wruhs' evaluation, 13 cases were rated as excellent, 7 as good, 2 as fair 1 year postoperatively. Conclusions LISS is a safe and effective fixation device for proximal tibial fractures, especially for those caused by high- energy injuries. The benefits of this system include less intervention of blood supply, less soft tissue complications and a high union ratio of fractures.
8.Studies on the correlation between XRCC2 gene polymorphism and the susceptibility to lung cancer in population of North China
Medical Journal of Chinese People's Liberation Army 1981;0(04):-
Objective To investigate the correlation between the polymorphism of XRCC2 gene,a DNA double-strand break repair(DDSBR)gene,and the susceptibility to lung cancer in population of North China.Methods By PCR-based restriction fragment length polymorphism(RLFP)technique(PCR-RLFP),a case-control study was performed among 300 patients with lung carcinoma and 300 healthy controls to detect XRCC2(C41657T)polymorphism.Results There was no significant difference in XRCC2(C41657T)allele frequencies between lung cancer patients and healthy controls(P=0.16).The C/C,C/T and T/T genotype frequencies were 72.7%,24.0% and 3.3% in lung cancer patients,and 76.3%,22.0% and 1.7% in healthy controls,respectively.There was no significant difference in C/C and C/T genotype frequencies between cancer patients and healthy controls(?2=0.48,P=0.48).However,the C/T genotype frequency in non-smoke group was significantly higher in lung cancer patients than in healthy controls(?2=6.67,P=0.01).The risk for lung cancer in non-smokers was 2.11 times higher in C/T genotype carriers than that in C/C genotype carriers.There was no significant difference in C/C and C/T genotype frequency between lung cancer patients and healthy controls in the smoke group(P=0.16).Conclusion Overall,there was no significant correlation between genetic polymorphism of XRCC2(C41657T)and the susceptibility to lung cancer.But the C/T genotype might increase the risk of suffering from lung cancer in no-smoking populations of Northern China.
9.Antisense oligonucleotides targeting TNF-? suppress on Co-Cr-Mo particle-induced osteolysis
Rui WANG ; Ting GUO ; Xiaofeng ZENG
Orthopedic Journal of China 2006;0(02):-
[Objective]To investigate the effect of a single subcutaneous dose of an antisense oligonucleotide(ASO) on particle-induced osteolysis.[Method]The murine calvaria osteolysis model was utilized in ICR mice.Bone resorption was measured with the toluidine blue staining.Osteoclasts were detected by tartrate resistant acid phosphatase(TRAP) staining assay and were quantified by a TRAP quantification kit.[Result]Bone resorption was 0.347 ? 0.09 mm2 in animals with particle implantation,and decreased to 0.123 ? 0.05 mm2 and 0.052 ? 0.02 mm2 after ASO treatment in low and high doses,respectively.The bone resorption was reestablished in animals given an additional TNF-?.The number of osteoclasts in animal calvaria treated with ASO was reduced obviously compared with those untreated animals and the quantification results indicated that about 90% osteoclastgenesis was suprressed by the ASO.Additionally,the osteoclastgenesis was reestablished by the addition of TNF-?.[Conclusion]An antisense oligonucleotide targeting an inflammatory factor,TNF-?,has been to suppress the osteolysis induced by particle for the first time.This new finding holds a great promise.It is a therapeutic strategy for the component loosening.
10.Effect of angelica on protein kinase C during myocardial ischemia reperfusion injury in rat
Yanlin WANG ; Chengyao WANG ; Rui ZENG
Chinese Journal of Anesthesiology 1996;0(08):-
Objective To study the molecular biological mechanism of the protective effect of angelica on myocardium during ischemia reperfusion Methods The myocardial ischemia/reperfusion was induced with the 40 min cross clamp/120 min declamping of anterior decending coronary artery Forty SD rats were randomly divided into 3 groups: group A without myocardial ischemic reperfusion , group B with intravenous adminisrtration of normal saline 0 8ml/100g before ischemia reperfusion , and group C with intravenous adminisrtration of 25% angelica 0 8ml/100g before ischemia reperfusion The content of protein kinase C (PKC) in cardiac myocyte was measured with immunohistochemical method, and the PKC activity with the isotope lable method Results Compared with those in group B, the myocardial infarct size reduced significantly in group C (P0 05) but increased obviously in group C (P0 05) The PKC activity was significantly higher in group C than that in group A(P