This paper reported a method of determination of phosphorus in human hair by Spectrophotometry of phosphomolybdenum Blue.Samples were decomposed by means of dry ashing.Vitamin C was used as a reducing agent.The conditions of ashing and colouration of phosphomolybdehum Blue,the measurement wavelength and the confidence of this meth od were also studied.

Objective To evaluate the diagnostic value of the combined detection of serum Fer,AFP and AFP-L3 in patients with primary hepatic cancer (PHC).Methods The serum levels of Fer,AFP and AFP-L3 were determined in 92 patients with PHC,84 patients with benign liver disease,and 45 control subjects.Results The PHC group serum Fer was (819.4±616.8) μg/L,AFP was (556.9±451.5) μg/L,AFP-L3 was (109.0±62.5) μg/L.The benign liver disease group Fer was (229.3±155.5) μg/L,AFP was (6.0±4.2) μg/L,AFP-L3 was (26.0±49.4) μg/L.The healthy control group Fer was (125.6±110.5) μg/L,AFP was (2.8±0.8) μg/L,AFP-L was (7.0±1.8) μg/L,there was significant difference between the three groups (all P ＜ 0.01).The sensitivity of PHC group alone in detection of serum Fer was 85.9 ％,the sensitivity of AFP was 71.0 ％,the sensitivity of AFP-L3 was 83.7 ％,the combined detection of Fer and AFP or AFP-L3 improved the sensitivity of the test to 90.2 ％ and 95.7 ％,the three combined detection sensitivity was 98.9 ％.Conclusion Combined detection of serum Fer,AFP and AFP-L3 is essential in the diagnosis of PHC.

Objective To study the correlation between scan orientation and the detectability of rotator cuff injury.Methods Different scan orientations of magnetic resonance imaging of the rotator cuff injury were analyzed in high magnetic field in 103 patients.Results The supraspinatus tendon and signal abnormities were obtained in 103 patients,the infraspinatus tendon and signal abnormities in 53 patients,subscapular tendon and signal abnormities in 18 patients,knolls tendon and signal abnormities in 15 patients.The oblique coronal MR imaging was clearest in supraspinatus tendon,while at the axial images was clearest in infraspinatus tendon.Conclusion Rotator cuff and the surrounding tissue injury could be clearly scanned in high magnetic field,the detectability of rotator cuff injury depended on different scan orientations of magnetic resonance imaging.

Objective The patients with non-small cell lung cancer ( NSCLC) are still at risk of brain metastasis after surgi-cal treatment.The study was to investigate the clinical characteristics and the risk factors for postoperative brain metastasis in patients with NSCLC in order to provide references for prophylactic cranial irradiation ( PCI) . Methods The clinical and prognostic data of 213 patients with NSCLC in our department of oncology from February 2008 to February 2012 were retrospectively analyzed.The occur-rences of postoperative brain metastasis in NSCLC patients with different characteristics were observed.Cox model was used to investi-gate the risk factors for postoperative brain metastasis in patients with NSCLC. Results The overall cumulative rate of brain metasta-sis was 23.9%(51/213) and the rates of brain metastasis at 1-year, 2-year, 3-year after surgery were 6.1%, 14.1%, 22.5% re-spectively.Univariate analysis showed that the rates of postoperative brain metastasis in NSCLC patients with different ages, histological types, tumor stages and CEA levels were statistically significant (P<0.05).Cox regression showed that adenocarcinoma (HR=2.00, 95%CI:1.11~3.64), lung cancer stageⅢ(HR=3.70, 95%CI:1.67~8.25) and high CEA (≥5.0μg/L)(HR=3.16, 95%CI:1.60~6.25) were risk factors for postoperative brain metastasis in patients with NSCLC.The survival time in NSCLC patients with brain metastasis was remarkably lower than that in patients without brain metastasis (P<0.05). Conclusion The NSCLC pa-tients with adenocarcinoma, III stage disease, or high CEA are more likely to develop brain metastasis after surgery, which should be the focus of PCI.

Alopeeia areata (AA) has been well recognized with familial tendencies, but the genetic basis of this clinical observation remains unknown. The cytokine interleukin-1 receptor antagonist (ILlra) is a potent anti-inflammatory protein that can prevent immune-mediated inflammatory response in the skin. We characterized a polymorphism within the gene for this cytokine ILlra in this study and tested the gene as a possible marker in patients with alopecia areata. We have determined allele frequencies of the polymorphic cytokine genes in a control population and a group of 72 patients with alopecia areata. The frequency of allele 2 of interleukin-1 receptor antagonist in patients with AA was significantly higher than that of control group. It suggests that interleukin-1 receptor antagonist gene may be a candidate gene or severity factor for alopecia areata.

Objective To discuss the effect of iohexol on the renal function in the old patients undergoing coronary angiography and/or percutaneous coronary imervention(PCI).Methods The changes of plasm Cr,BUN before and after coronary angiography and/or PCI of the 60 old patients with the indicatmns of coronary angiography were compared and analyzed.Results The plasm Cr,BUN in 60 cases were fluctuated,but were not significantly changed(P＞0.05).Conclusion Iohexol has little effect on the renal function in the old patients undergoing coronary angiography and/or percutaneous coronary intervention(PCI).

Objective To discuss the effect of stress hyperglycemia on prognosis of nondiabetic patients with acute myocardial infarction. Methods 80 nondiabetic patients with acute myocardial infarction(AMI) were divided into 2 groups according to the early blood glucose level. Group A:blood glucose ≥ 8mmol/L;group B: < 8mmol/L. Serious cardiac arrhythmia, backward heart failure, cardiac shock and death incidence and so on were observed. Re-sults The incidences of serious cardiac arrhythmia,backward heart failure,cardiac shock and death were significantly higher in patients with stress hyperglycemia than those of patients without stress hyperglycemia(35% vs 13%, 38% vs 17%, 19% vs 2%, 31% vs 11%, P <0.05). Conclusion The incidences of serious cardiac arrhythmia,backward heart failure,cardiac shock and death in patients with stress hyperglycemia were significantly higher than those of pa-tients without stress hyperglycemia,which could be used as the parameters to judge the prognosis of patient.

Objective:To compare the choriocapillaris flow density and choroidal vessel volume (CVV) of acute and chronic central serous chorioretinopathy (CSC).Methods:A cross-sectional observational clinical study. A total of 64 eyes of 64 patients (CSC group) diagnosed with CSC at Department of Ophthalmology of West China Hospital of Sichuan University from May 2019 to October 2020, and a total of 64 eyes of 64 age and gender matched healthy volunteers (control group) during the same period were included in this study. In the CSC group, there were 34 patients with acute CSC (acute CSC group) and 30 patients with chronic CSC (chronic CSC group). There was no significant difference in age ( t=-0.041) and sex composition ratio ( χ2=0.191) between CSC group and control group ( P>0.05). There were statistically significant differences in age ( t=-1.872) and sex composition ratio ( χ2=8.778) between acute CSC group and chronic CSC group( P<0.05). Swept source optical coherence tomography angiography (SS-OCTA) was performed using VG200D. The scanning mode was 512×512 and scannig range was 12 mm × 12 mm. The choriocapillaris flow density of the 3 mm, 6 mm, 12 mm circular area and 1-3 mm ring, 3-6 mm ring, and 6-12 mm ring, and the CVV of the of the 3 mm, 6 mm, 12 mm circular area was automatically generated by the built-in software (v1.28.6). The age, choriocapillaris flow density and CVV were compared between two groups using independent sample t test. Results:Compared with the control group, the choriocapillaris flow density decreased in the CSC group, and there were statistically significant differences in the 3 mm, 6 mm circular area ( t=-7.210, -4.040; P<0.001). There were statistically significant differences between CSC group and control group in the 3 mm, 6 mm, 12 mm circular area ( t=1.460, 12.270, 11.250; P<0.05). Compared with the acute CSC group, the choriocapillaris flow density decreased in the chronic CSC group, and there were statistically significant differences ( P<0.05) in the 3 mm, 6 mm circular area ( t=3.230, 2.330), the total and four quadrants of 1-3 mm ring ( t=2.780, 2.060, 2.140, 2.620, 3.770), the superior quadrants of the 3-6 mm ring ( t=2.550), and the superior and temporal of 6-12 mm ring ( t=3.070, 2.610). There was no significant difference of CVV in the 3 mm, 6 mm and 12 mm circular area between the acute CSC group and the chronic CSC group ( t=0.250, 0.070, -0.110; P>0.05). Conclusion:Compared with acute CSC, chronic CSC exhibits significant decreased choriocapillaris flow density and no change in CVV.

AIM:To explore the characteristics of hepatitis B virus S gene mutation in the vertical transmission after active and passive vaccination .METHODS:Fifteen cases of immunoprophylaxis failure were enrolled in the study . HBV S gene (including pres-S and S) from the mothers, newborns before active and passive vaccination and 7-month-old infants with immunoprophylaxis failure were detected by PCR amplification .The characteristics of HBV S gene mutation were compared among the 3 groups.RESULTS: The genotype of HBV in the newborns and the infants was the same as that in the mothers .The frequencies of mutation in the 2 fragments of the HBV S gene had no significant difference between the 3 groups.The homology tree model based on HBV S gene was analyzed in the 3 groups, in which every group had their own cluster.There were 15 different mutation sites between 7 pairs of mothers and newborns .There were 3 different muta-tion sites between 3 pairs of newborns and infants (nt273A→A/G, nt512C→C/T and nt1139C→A), among which the first 2 were located in the S gene region but not in the “a” determinant , and the latter was located in the overlap region of S and X genes .There were 25 different mutation sites between 9 pairs of mothers and infants , but only 1 case had a differ-ent mutation site between the mother , newborn and infant .CONCLUSION: The HBV species in newborns and infants with immunoprophylaxis failure were transmitted from the mothers .The mutations in the HBV S gene with immunoprophy-laxis failure happened before and after active and passive vaccination , mainly before vaccination .The relationship between HBV S gene mutations and immunoprophylaxis failure should be further explored .

Aim To elucidate the biochemical mechanism of gossypol in inducing the decline of sperm quality.Methods Gossypol was administered orally at the dose of 50 mg?kg-1/2 d for two weeks.Then,the sperm was collected from the left caudal epididymis and was analysed by CASA.The morphological changeand the concentration of nitric oxide(NO)in testes as well as the level of hormone〔follicle-stimulating hormone(FSH)〕,luteotrophic hormone(LH),testosterone(T)in serum were assayed.Results Gossypol could induce the decrease of sperm number and sperm quality.The concentration of NO in testes increased significantly.Among the three kind of hormone,only the concentration of T showed decrease after the oral administration of gossypol.NA1108 could antagonize the decline of sperm quality damaged by gossypol and decrease the content of NO in testes.Conclusions The concentration of NO in testes beyond normal value was one of the toxic mechanism of gossypol that contributed to the inhibition of spermatogenesis.Some drugs with the ability to reduce NO content in testes could also increase sperm quality.