Objective To establish a method for the determination of Ca2 + cations in the nutritive food by ion chromatography.Methods After being digested by the mixture of nitric acid and perchloric acid,the Ca2+ cations in the nutritive food were determined by ion chromatography(IC).Results The optimum chromatographic conditions were studied thoroughly,the linear range was 0.10-500 mg/L,r=0.9998.The lowest detection limit of Ca2+ cations in nutritive food was 0.02 mg/L,RSDs were 0.50%-4.00%,the rates of recovery tested by the standard addition method were in the range of 95.0%-103.0%.Conclusion This method is simple,fast,sensitive and accurate.

Objective To assess the efficacy and safety of multimedia visual training system for ambly-opic children through a randomized, controlled clinical trial. Methods A total of 203 amblyopic children (253 eyes) ages 3 to 12 years were randomly divided into a study group (100 patients, 170 eyes) and a control group (103 patients, 183 eyes). The study group received multimedia training system; while the control group received conventional integrated therapies. Best corrected visual acuity was examined 3 and 6 months after treatment. The efficacy and safety of multimedia visual training system for amblyopic children was assessed by comparing the ef-fect of treatment in both groups. Results 6 months after treatment, the total effectiveness rate was 74.1% in the study group and 52.5% in the control group, and there was a statistical difference (P < 0.0001). The effective-ness rate for refractive amblyopia was higher in the study group than in the control group (80.5% vs. 50.8%), and there was a significant difference (P < 0.001). As compared with the effectiveness rate for different ages, the subgroups of 3 to 6 years and 7 to 12 years in the study group was 80.8% and 58.0%, much higher than those in the control group (54.9% and 43.9%), and there were statistical differences(P < 0.001 and P < 0.05). Conclusions The efficacy of multimedia visual training system is superior to that of the traditional treatment , especially for refractive amblyopia. It is effective in all age groups.

Objective To establish a method to determine dicofol and eight pyrethroid pesticides residues in vegetables and fruits by gas chromatography.Methods The crushed samples were extracted with organic solvent,then the extract was analyzed by DM-17 capillary column gas chromatography after being purified by PestiCarb-NH2 cartridge and silica cartridge solid phase extraction.Results The detection curve covered the linear range of 0.10-2.0 ?g/ml .The minimum detection limit was 0.011-0.090 ?g/kg.The rates of recovery were 85.4%-105%.The variable coefficient(RSD) was under 7.0%.Conclusion This method has the advantages of less disturb, simple and high accuracy, and it can be applicable to the routine analysis of dicofol and pyrethroid pesticides residues.

OBJECTIVE:To improve the formulation of Kangfu cream,in order to increase the stability of it. METHODS:Ac-cording to the problem that demulsion of zinc oxide and poor stability of Kangfu cream,orthogonal test was used to optimize the formulation of cream matrix with oil phase ratio (stearic acid-white vaseline-glycerol monostearate),with the amount of polysor-bate 80,triethanolamine and SG-10 as factors,using comprehensive score of glossiness,delicacy,consistence,coating and stratifi-cation(10 points as full mark)as index. The stability of the cream was validated and investigated,such as compatibility,heat-re-sisting,cold-resistant,lighting. RESULTS:The formulation were as follows as 100 g cream containing stearic acid 8 g,white vase-line 8 g,glycerol monostearate 6 g,polysorbate 80 4 g,triethanolamine 3 g,SG-10 2 g and appropriate amount of water. The comprehensive scores of the cream were >9.5 points (n=3). Compared with the cream prepared by previous formulation,the cream prepared by modified formulation had no significant change in appearance and color,and demulsion and layering hadn’t been found. CONCLUSIONS:Kangfu cream with better stability than original formulation is successfully prepared.

Adenomatous Polyps ; diagnostic imaging ; etiology ; Aged ; Aged, 80 and over ; Colorectal Neoplasms ; diagnostic imaging ; etiology ; Female ; Humans ; Liver ; diagnostic imaging ; Male ; Middle Aged ; Non-alcoholic Fatty Liver Disease ; complications ; diagnostic imaging ; Ultrasonography

Objective:To revise the Chinese version of Devereux Early Childhood Assessment for Preschool-ers,Second Edition (DECA-P2 ),and assess its validity and reliability.Methods:Totally 608 children aged 3 -5 years were rated by their teachers with the Chinese version of DECA-P2 and two criterion scales,which included the Chinese version of Preschool Behavioral and Emotional Rating Scale,and Social Competence and Behavior Evalua-tion Scale-30.Data of 570 were valid.After identifying valid items using item analysis,exploratory factor analysis was performed for half of all samples.Confirmatory factor analysis was also conducted with another half samples.Moreover,the present study tested criterion-related validity,internal consistency reliability and split-half re-liability of the Chinese version of DECA-P2.Results:Through the exploratory factor analysis,the obtained structure of protective factors was the same as the original scale,accounting for 59.82%of the variance.The results of con-firmatory factor analysis achieved the standard of psychometrics (χ2/df=2.50,RMESA=0.07,CFI=0.91,IFI=0.91).Generally,each subscale had significant correlation with the two criterion scales (r=-0.21 ~0.80). Be-sides,the Cronbach αcoefficients of all subscales were between 0.80 and 0.93,and the split-half reliabilities of them were between 0.79 and 0.89.Conclusion:The Chinese version of DECA-P2 has acceptable psychometric quality and can be applied to evaluate resilience for children aged from 3 to 5 years in China.

Objective To approach the alleviating effect of medicative diet for food therapy on hepatic function of patients with gastric cancer and adjuvant chemotherapy after operation and supply references for effective treatment and nursing. Methods We divided 123 patients with gastric cancer after operation into the test group (65 cases) and the control group (58 cases).The control group adopted routine treatment project while the test group received medieative diet for food therapy based on routine treatment project. The changes in hepatic functions were compared between the two groups. Results The changes in hepatic functions of the test group were better than those of the control group (P<0.01 ). Conclusion Medicative diet for food therapy could significantly restore the seriously damaged hepatic function after chemotherapy.

To determine the content of dendrobine in Dendrobium nobile from different harvest times and plant parts, to research the inherent rule about it. GC with internal standard was used to determine. The content of dendrobine had significant differences in different periods and parts. The dendrobine content is higher in four-year root than in three-year root. The dendrobine content in the upper segment of stem is the highest, secondly is in the middle seg-ment, and in the low segment is the lowest. This offered evidence to determine the most appropriate harvest time and fair use of different parts for D.nobil.

Objective To observe the changes and clinical significance of procalcitonin(PCT) level in children with bacterial pneumonia.Methods The levels of PCT,hs-CRP and WBC were detected in 31 pneumonia children pre and post antibiotic treatment,and the changes were observed.Results The PCT levels of the 31 patients were all increased before treatment.After cured,PCT level of 28 cases returned to normal.But in the 28 normal cases,WBC level increased in 10 cases(35.7％),and hs-CRP level elevated in 4 cases(14.3％).In the other 3 cases,the PCT levels decreased from 5.69ng/mL,1.25ng/mL and 0.83ng/mL at admission to 0.51ng/mL,0.55ng/mL and 0.52ng/mL,respectively.And in these 3 cases,there was one case with the levels of WBC and hs-CRP respectively ascended,in which WBC level descended from 13.1 × 109/L to 12.1 × 109/L and hs-CRP level reduced from 20.92mg/L to 7.38mg/L.Conclusion The sensitivity of the index PCT is the highest at the accurate time phase of bacterial pneumonia,which can guide the reasonable use of antibiotics.

Objective To retrospectively review and compare the clinical results of allogeneic peripheral blood stem cell transplantation (allo-PBSCT) from HLA- matched sibling donors mobilized with different regimens. Methods Seventy-one patients with hematological malignant diseases received allo-PBSCT from HLA-matched sibling donors in our department. Among them, 24 received allografts mobilized with G-CSF (group G), and the remaining (47 cases) were mobilized with G-CSF and GM-CSF (group G+ M). CD34+ subsets and T cell subsets in the allografts were analyzed, and the time of hematopoietic reconstitution and the incidence of graft versus host diseases (GVHD) were compared. The adverse effects on the donors after mobilization were also observed. Results The enough targeted CD34+ cells in all donors were harvested by 1-3 aphereses. Ninety-six h after mobilization, WBC counts of the donors were significantly higher in group G than in group G + M [(49. 6± 19. 5) 109/L vs (25.4 ± 10. 4) 109/L, P＜0. 05]. Analysis of the CD34+ subsets showed that the percentage of cells with the CD34+/CD38- phenotype was significantly higher in group G + M than in group G [(37. 7 ± 5. 7) % vs (31.4 ± 4. 5) %, P＜0. 05]. There was no significant difference in T cells and subsets of grafts. There was no significant difference in the number of total CD34+ cells and CD34+ CD38- cells, and infusion of T cells between two groups. The days required for the recovery of neutrophils and platelets was inversely correlated with the infused CD34+ and CD34+ /CD38- cell number. There was no significant difference in incidence of acute and chronic GVHD between two recipient groups. Seventeen cases and 10 eases among 71 eases died of relapses of primarydiseases, and complications of transplantation such as severe GVHD and infections respectively.Fourteen cases in group G (58.3 %) and 31 cases in group G+ M (66.0 %) survived. The most common adverse events in the donors were bone pain and fever, which mostly occurred 36 h after mobilization and could be relieved by non-steroidal anti-inflammatory drugs. Conclusion Two mobilization regimens showed equivalent clinical results. But the combined regimen of G-CSF and GM-CSF demonstrated a significantly greater mobilization of cells with the CD34+/CD38- phenotype.Meanwhile in allogeneic PBSCT, a greater number of total CD34+ cells and CD34+ CD38- cells infused may be associated with faster hematopoietic reconstitution of recipients.