Objective To explore the effect and lung function of the budesonide combined with montelukast for treatment of chronic obstructive pulmonary disease(COPD)and the effect on serum IL-6,IL-8 and TNF-α.Methods 120 patients with COPD in our hospital from April 2012 to December 2013 were randomly divided into observation group and control group (n =60 ).Observation group was given budesonide combined with montelukast on the basis of general routine treatment and control group was given budesonide on the basis of general routine treatment.The lung function and the level of IL-6、IL-8 and TNF-αin serum were detected berore and after treatment.Results The efficacy in observation group was significantly better than that in control group(P<0.05).The forced vital capacity(FVC),forced expiratory volume in one second(FEV1),maximal mid expiratory flow(MMEF),and peak expiratory flow(PEF)in the observation group were significantly higher than those in the control group(P<0.05 ).In addition, after treatment the levels of IL-6,IL-8 and TNF-αexpression in serum of the observation group and control group were significantly lower than before treatment,but the levels of the observation group after treatment was significantly lower than the control group,the difference were statistically significant (P<0.05).Conclusion The efficacy of the budesonide combined with montelukast for treatment of COPD is exact by reducing cytokine release, controling airway inflammation to improve the lung function.It is worthy to be popularized.

Since the release rate of protein in hydrogels is directly dependent upon the size of the protein and the hydrogel, how to deliver low molecular weight protein for prolonged periods has always been a problem. In this article, we present a usage of self-assembling peptide (P3) with the RGD epitope on its N terminus. The concentration of the released insulin-like growth factor 1 (IGF-1) was determined by UV-vis spectroscopy and the release kinetics suggested a notable reduction of the IGF-1 release rate. Cell entrapment experiments revealed that IGF-1 delivery by biotinylated nanofibers could promote the proliferation of the mouse chondrogenic ATDC5 cells when compared with cells embedded within nanofibers with untethered IGF-1.
Animals ; Biotin ; Cell Line ; Delayed-Action Preparations ; Drug Carriers ; chemistry ; Hydrogels ; chemistry ; Insulin-Like Growth Factor I ; pharmacology ; Mice ; Nanofibers ; Oligopeptides ; chemistry

Talent training is the foundation for discipline construction and it is also the fundamental guarantee of hospital's sustainable development.The affiliated Zhongshan hospital of Fudan university launched investigations from the teaching management,teaching staff,resident and clinical skills to look for problems in clinical teaching and to propose improvement measures aiming at improving the clinical teaching and promoting hospital resident and specialists training.

Aim To investigate the effects of total gin-senosides ( TG) on microvascular regeneration and car-diac function in rat after acute myocardial infarction ( AMI ) . Methods The acute myocardial infarction model was created with left coronary artery ligated in male Sprague Dawley rats. The model rats were ran-domly divided into sham, model, TG low and high dose groups. TG groups were injected into abdominal cavity with TG(20 mg·kg-1·d-1, 40 mg·kg-1· d-1 ) . Sham and model groups were injected with e-qual-volume normal saline. On the 35th day post-opera-tion, heart function was examined by color doppler ul-trasoundination. HE, Masson and immunohistochemis-trical staining were used to observe the histopathologi-cal changes of myocardium and micro vessel density. The level of vascular endothelial growth factor( VEGF) and basic fibroblast growth factor( bFGF) mRNA were detected by real-time PCR. Results Compared with the model group, high and low dose TG obviously de-creased the left ventricular end diastolic dimension, the left ventricular end-systolic dimension, the left ventric-ular end-diastolic volume and the left ventricular end-systolic volume(P<0. 05 and P<0. 01), and signifi-cantly increased the ejection fraction and the fractional shortening ( P <0. 01 ) . The histopathological changes of myocardium on myocardial infarction and fibrosis were dramatically reduced by TG. But ventricular wall was thicker. Two dose TG remarkably increased the expressions of VEGF and bFGF mRNA and micro ves-sel density of compositive CD31 + cells in ischemic myocardial tissue and around of infarct area(P<0. 01, P <0 . 0 5 ) . Conclusions TG could improve the car-diac function of acute myocardial infarction rat. The mechanism may be related to the upregulation of VEGF and bFGF gene expression, the promotion angiogene-sis, then the improvement of blood supply in myocardi-al infarction area.

Objective To investigate the effects of Modified Siwutang aqueous extract (mSWT) on bone marrow cell mobilization and plantation in mice after acute myocardial infarction (AMI).Methods The AMI model was established by ligating the left anterior descending artery in Kunming mice.Bone marrow cells were marked by injection of DAPI into the cavitas medullaris of shin bones.Mice were administered with mSWT 60 mg/kg by intragastric administration once a day after surgery.After haematoxylin-eosin and immunofluorescent staining,the plantation of bone marrow cells in AMI area and microvessel density were detected by histopathological assay.The EPCs proportion of peripheral blood was measured by flow cytometry.Results Compared with model mice,infarct size obviously was decreased after mSWT 60 mg/kg treatment,with EPCs ratio increased significantly.Inflammatory cell infiltration of myocardium was much less.Myocardium cellular degeneration and necrosis,fibrosis were reduced,which survival cardiac muscle tissue was increased.DAPI positive cells of plantation and survive were found in AMI region of mSWT group.The number of positive cells were increased in mSWT group than that of model group.There were lot of microvessels of vWF positive expresseion in mSWT than model group.Conclusion:mSWT significantly improved histopathology of AMI mice.The mechanism may be involved in moved bone marrow cells,homed to AMI area and promote angiogenesis.

Patients in intensive care unit were more critically ill, and often accompanied by dottiness and restless. In order to avoid the adverse events and self-injury, body constraints were often used on the patients. Therefore, this paper would discuss the ethics of body constraints and give the suggestions. According to the literature review and the analysis of the clinical nursing experience of relevant ethical principles, data was collected. The principle of informed consent, respect, and harmless was hard to decide on the issue of body constraints for patients in ICU. Nurses might pay attention to the application of ethical principles, and strengthen the humanistic care. On the issue of body constraints for ICU patients, nurses might pay special attention to the application of possible ethics principles in nursing process and reduce the application of the body constraints, and promote the physical and mental comfort of patients. It reflected the progress of the humanistic solicitude in the current clinical nursing and important value.

Abstract: Objective To investigate the effect of lifting operation on fatigue susceptibility of local muscles under different Methods liftingmass,frequencyandangle. Fifteenmalecollegestudentvolunteerswereselectedasthestudysubjectsusinga- purerandomsamplingmethod.Eightkindsofliftingoperations(setasA Hoperationgroup)wereperformedunderthespecified mass (5 and 10 kg), frequency (10 and15 times/min) and angle (45° and 90°), five minutes each. The percentage of maximum voluntaryelectricalactivation(MVE%)andaveragepowerfrequency(MPF)oferectorspinaeandmultifidusmuscleatmaximumResultsvoluntarycontractionweremeasuredbysurfaceelectromyography.TherewasanassociationbetweentheleftandrightP0.05).Theresults ofmultiplelinearregressionanalysisshowedthattheprimaryinfluencingfactoroffatigueofleftandrighterectorspinaeandleft multifidusmusclewasfrequency,followedbyquality,andtheinfluencingfactorsoffatigueofrightmultifidusmuscleweremass, angleandfrequencyindescendingorder.TherewasnosignificantdifferenceinMPFvalueofGgroupwiththechangeofliftingPP0.05),butthereweresignificantdifferencesinMPFvalueofothergroupswiththechangeofliftingtimes(all0.01). ThelinearregressiondeclineofMPFwasthemostobviouswiththechangeofliftingtimeswhentheliftingweightwas 10kg,the Conclusion frequency was 10 times/min, and the angle was 90°. In the lifting operation of left turn, the erector spinae and multifidusmusclesaremostlikelytobecomefatiguewith 10kgliftingmass,10timesperminfrequency,and90°angle.Lifting

Abstract: Objective To investigate the effect of lifting operation on fatigue susceptibility of local muscles under different Methods liftingmass,frequencyandangle. Fifteenmalecollegestudentvolunteerswereselectedasthestudysubjectsusinga- purerandomsamplingmethod.Eightkindsofliftingoperations(setasA Hoperationgroup)wereperformedunderthespecified mass (5 and 10 kg), frequency (10 and15 times/min) and angle (45° and 90°), five minutes each. The percentage of maximum voluntaryelectricalactivation(MVE%)andaveragepowerfrequency(MPF)oferectorspinaeandmultifidusmuscleatmaximumResultsvoluntarycontractionweremeasuredbysurfaceelectromyography.TherewasanassociationbetweentheleftandrightP0.05).Theresults ofmultiplelinearregressionanalysisshowedthattheprimaryinfluencingfactoroffatigueofleftandrighterectorspinaeandleft multifidusmusclewasfrequency,followedbyquality,andtheinfluencingfactorsoffatigueofrightmultifidusmuscleweremass, angleandfrequencyindescendingorder.TherewasnosignificantdifferenceinMPFvalueofGgroupwiththechangeofliftingPP0.05),butthereweresignificantdifferencesinMPFvalueofothergroupswiththechangeofliftingtimes(all0.01). ThelinearregressiondeclineofMPFwasthemostobviouswiththechangeofliftingtimeswhentheliftingweightwas 10kg,the Conclusion frequency was 10 times/min, and the angle was 90°. In the lifting operation of left turn, the erector spinae and multifidusmusclesaremostlikelytobecomefatiguewith 10kgliftingmass,10timesperminfrequency,and90°angle.Lifting

Abstract: Objective To investigate the effect of lifting operation on fatigue susceptibility of local muscles under different Methods liftingmass,frequencyandangle. Fifteenmalecollegestudentvolunteerswereselectedasthestudysubjectsusinga- purerandomsamplingmethod.Eightkindsofliftingoperations(setasA Hoperationgroup)wereperformedunderthespecified mass (5 and 10 kg), frequency (10 and15 times/min) and angle (45° and 90°), five minutes each. The percentage of maximum voluntaryelectricalactivation(MVE%)andaveragepowerfrequency(MPF)oferectorspinaeandmultifidusmuscleatmaximumResultsvoluntarycontractionweremeasuredbysurfaceelectromyography.TherewasanassociationbetweentheleftandrightP0.05).Theresults ofmultiplelinearregressionanalysisshowedthattheprimaryinfluencingfactoroffatigueofleftandrighterectorspinaeandleft multifidusmusclewasfrequency,followedbyquality,andtheinfluencingfactorsoffatigueofrightmultifidusmuscleweremass, angleandfrequencyindescendingorder.TherewasnosignificantdifferenceinMPFvalueofGgroupwiththechangeofliftingPP0.05),butthereweresignificantdifferencesinMPFvalueofothergroupswiththechangeofliftingtimes(all0.01). ThelinearregressiondeclineofMPFwasthemostobviouswiththechangeofliftingtimeswhentheliftingweightwas 10kg,the Conclusion frequency was 10 times/min, and the angle was 90°. In the lifting operation of left turn, the erector spinae and multifidusmusclesaremostlikelytobecomefatiguewith 10kgliftingmass,10timesperminfrequency,and90°angle.Lifting

A sensitive and selective method based on gas chromatography hyphenated to mass spectrometry (GC-MS) was developed and validated for the determination of atractylon in rat plasma. Plasma samples were processed by liquid-liquid extraction with ethyl acetate-n-hexane (1:1, v/v) using acetophenone as an internal standard (IS). Analytes were determined in selective ion monitoring (SIM) mode using target ions at m/z 108.1 for atractylon and m/z 105.1 for acetophenone. The calibration curve was linear over the concentration range of 10-1000 ng/mL with lower limit of quantification of 10 ng/mL. The intra- and inter-day precision variations were not more than 10.4% and 9.6%, respectively, whilst accuracy values ranged from -6.5% to 4.9%. Extraction recovery of the assay was satisfactory. This method was suc-cessfully applied to quantification and pharmacokinetic study of atractylon in rat plasma after in-tragastric administration of Atractylodis extract.