Objective To investigate the method and application of measurement uncertainty in clinical biochemical tests .Meth-ods The synthetic uncertainty and expanding uncertainty of 13 emergency biochemical tests items were compared between Hitachi 7600 and Beckman DXC800 automatic biochemical analyzer by usingmodel method.The test items included aspartate aminotrans-ferase ,total protein ,albumin ,glucose ,urea ,creatinine ,creatine kinase ,lactate dehydrogenase ,amylase ,potassium ,sodium ,calcium and chloride .Results Measurement uncertainty of aspartate aminotransferase and lactate dehydrogenase were 3 .66% and 3 .51%respectively ,which were the top two on Hitachi 7600 analyzer ;measurement uncertainty of calcium and creatinine were 4 .00% and 3 .90% respectively ,which were the top two on Beckman DXC800 .Compared between the two analyzers ,the test item with the lar-gest difference of measurement uncertainty was sodium(2 .62% ) ,and the second was creatinine(2 .40% ) .The difference of meas-urement uncertainty of the 13 emergency biochemical test items between the two analyzers were relatively small .Conclusion The two analyzer both have good performance ,Model methodis suitable for the evaluation of regular test items′measurement uncer-tainty in clinical biochemical laboratory .

The normal and ischemic dog myocardia were treated with freeze-etching method and observed by transmission electron microscope, with special reference to the ultrastructural changes of myocardial cell membrane.Following 6 hours of coronary occlusion, the myocardial samples were taken from central and borderline ischemic areas as well as from distant normal area. The samples were cut into slices of about 1.5?1.5?3 mm~3 in size and proceeded by the routine freeze etching procedure.The myocardial cell membrane were fractured into the P face and E face. In normal myocardial cell membrane, the number of intramembranous particles of the P face was exceedingly larger than that of the E face. There were numerous caveolae appearing as depressions on the P face and elevated structures on the E face. In central and borderline ischemic areas, intramembranous particles were significantly decreased and aggregated. Following coronary occlusion, the caveolae of the myocardial cell membrane were found to be decreased, deformed or absent. In the severe ischemic area, spherical elevations with "rosette" configuration were occasionally observed as elevation on P face and depressions on E face.

The normal rabbit pancreas was treated with DMSO freeze-cracking method and observed by scanning electron microscope, with special reference to examine the ultrastructure of pancreatic exocrine portion.The pancreatic acinus was composed of five to six pyramidal acinar cells around the lumen. A few short microvilli were present at the apex of each acinar cell. The spherical nucleus was located in the basal region, with one to three nucleoli.Numerous rough surfaced endoplasmic reticula were found in the basal region of acinar cells arranged either in longitudinal arrays, or concentrically. Mitochondria were located between rough surfaced endoplasmic reticula. The rough surfaced endoplasmic reticulum may extend into supranuclear portion where well-developed Golgi apparatus and numerous spherical zymogen granules were situated.

Ten novel conjugates with ursolic acid core and different chalcone ligands were synthesized via esteri-fication using the natural ursolic acid as starting material. The structures of these conjugates were confirmed by 1 H NMR, 13 C NMR and HRMS. The preliminary biological results showed that these compounds displayed signifi-cant antiproliferative effect on CNE2, KB, MCF-7, A549 and HepG2 cells. These compounds were more effective than ursolic acid and tamoxifen against MCF-7 cells. Especifically, compound 11e ( IC50 =4. 7 μmol/L) showed the greatest potency against MCF-7, which was about 3-times more potent than tamosifen ( IC50 =15. 2 μmol/L) . Additionally, all conjugates were nontoxic to health MCF-10A and VERO cells, and had higher security than tamoxifen.

To investigate the influence of Bufei Jianpi Granule (BJG) on immune function in mice. Spleen cellular proliferation and the activities of IL-1 and IL-2 in mice were observed. The differences of spleen cellular proliferation stimulating by Con A, the secretion of IL-2 from spleen cells and the secretion of IL-1 from celiac macrophage were significant among BJG group, the control group and the water-drinking group. [Conclusion] BJG can promote the function of cellular immune and the secretion of IL-1 and IL-2 from immune active cells in mice.

To study the therapeutic effectiveness of “Gongxuening Capsule” in the treatment of prolonged lochia. The therapeutic effectiveness of “Gongxuening Capsule” was observed in the treatment of prolonged lochia to compare with the use of antibiotic combined with Yimucao electuary. Out of 42 cases treated, significant therapeutic effectiveness was observed in the treatment of prolonged lochia by using “Gongxuening Capsule”, with the total effective rate of 95 2%. The result was better than the group of patients in whom antibiotic combined with Yimucao electuary was used ( P

Objective To explore the preference of emotional memory in different cognitive style of undergraduates.Methods A 2 cognitive style (field independent style / field dependent style) * 3 facial expressions (positive/neutral/negative faces) mixed designs were adopted to compare the reaction time,which in the condition of stimuli presentation time 500 ms in field independent and dependent participants (n =25,respectively).E-Prime program was used to present the stimulus and to record the reaction time and accuracy to stimuli of all subjects.Repeated Measurement ANOVA was used in the research,facial expressions (positive/neutral/negative faces) as the within group factor,and groups (stress/non stress) as between group factor.Both the main effect of each variable and the interaction effect of variables were tested.Results There were a significant difference of response time and accuracy between the field independent and field dependent college students.It was shown by repeated measurement A NOVA that the main effect(F(2,47) =4.321,P＜ 0.05)in facial expressions properties and the interaction effect(F(2.47) =3.299,P＜ 0.05)between groups and facial expressions properties were found.Further analysis showed that the reaction time to detect stimulus of positive((610.71 ± 11.26)ms)and neutral ((606.24 ± 18.27) ms) facial expression was shorter than negative ((653.17 ± 20.91) ms) faces for field independent participants (P＜0.05).Interestingly,for field dependent group,the neutral faces ((675.67 ± 21.01) ms)were faster than others (P ＜ 0.05).Conclusion Field independent students had significant advantages over field dependent students in emotional memory.

Objective To compare the influence of propofol combined with sufentanil or remifentanil on the quality of wake-up during scoliosis surgery by wake-up test.Methods Fifty pa-tients undergoing scoliosis surgery were randomized into two groups.During the surgery,propofol combined with sufentanily 0.3-0.6 μg·kg-1·h-1 (group SF)or remifentanil 0.2-0.3 μg·kg-1·min-1 (group RF)were continuously infused to maintain anesthesia,and BIS was maintained at 40-60.In wake-up test,the infusion of sufentanyl in group SF was paused and,the infusion rate of remifentanil in group RF was adjusted to 0.05 μg·kg-1·min-1 until the patient completed the wake-up test under instruction.The time that spontaneous breathing occurred,body movement was detected and the capa-bility to follow instructions in both two groups were recorded.MAP,HR,PET CO2 were measured at the time 10 min after medication adjustment (T1 ),waking up(T2 )and 10 min after waking up (T3 ), respectively,in both two groups.Wake-up quality was also recorded.Results The time that sponta-neous breathing occurred,body movement was detected and the capability to follow instructions in group RF were significantly shorter than those in group SF (P <0.05).At T2 the incidence of agita-tion in group RF was significantly higher than that in group SF(P <0.05).And the hemodynamics of group SF were more stable than those of group RF (P <0.05).Conclusion Propofol combined with sufentanil can improve wake-up quality during scoliosis surgery,but the wake-up time is relatively lon-ger.

Objective To summarize the clinical experience of nasal endoscopic treatment for refractory epistaxis. Methods .A retrospective analysis was made on 258 patients with refractory epistaxis treated with intranasal endoscopic exploration and hemostatic treatment from January 1998 to December 2004 in this hospital. Results .Hemorrhage was found on the nasal septum and the lateral nasal wall in 118 and 47 patients, respectively. Nose bleeding resulted from intranasal tumor in 8 patients. The site of bleeding was not clearly identified in 85 patients. Of the 258 patients, 257 responded to the treatment (99.6%) and 1 died ( 0.4%).Recurrent bleeding occurred in 43 patients. The incidence of recurrent bleeding was significantly higher in patients with unknown bleeding sites than in clearly diagnosed patients (?2=17.689,P=0.000). Conclusions .The emphasis of nasal endoscopic treatment for refractory epistaxis should be laid on the initial management, during which the bleeding site should be carefully identified. For patients with repeated nose bleeding, a combination treatment, including endoscopic filling-up of the anterior nasal cavity, endoscopic embolization of anterior and posterior nose, or embolization of the internal maxillary artery, is necessary.

Objective To clone CD34 extracellular region encoding cDNA and to construct its eukaryon expression vector to explore the feasibility of its monoclonal antibody preparation by gene immunization. Methods Total RNA was extracted from KG-1a cell lines. CD34 extracellular region encoding genes were amplified by RT-PCR method and then confirmed by enzymatic lysis and DNA sequencing, and its eukaryon expression vector was constructed as pcDNA3.1-CD34. Twelve BABL/c mice aged 4-6 weeks were selected and randomly divided into 3 groups. Before immunization, 50?l 25% saccharin solution was injected into mouse musculus quadriceps femoris. Fifteen minutes later, blank control PBS, PBS diluted empty vector pcDNA3.1(50?g), or PBS diluted pcDNA3.1-CD34 was injected into the same site of the above three groups, respectively. Immunization was taken every two weeks for a total of three times. The antibody was detected regularly by FACS using tail blood from immunized mice. Results The results demonstrated that the length of CD34 cDNA was 886bp which was identical to the theoretical value and its sequence was confirmed by DNA sequencing which was identical to the registered sequence. The accuracy of CD34 expression vector recombination was confirmed by restriction enzymatic lysis. Hind III and EcoRI restriction enzymatic lysis sites were introduced into 5 and 3 terminals of amplified cDNA sequence, respectively. Terminate code TGA was also introduced into CD34 extracellular encoding cDNA. The expression vector possessing target genes was named as pcDNA3.1-CD34. FACS detection indicated that only 1/4(25%) immunized mice had a lower titer CD34 antibody in their tail vein serum 2-6 weeks after immunization, and the others did not show no antibody production. Conclusion The eukaryon expression vector pcDNA3.1-CD34, which express CD34 extracellular region, has been constructed. The feasibility of CD34 McAb preparation can primarily be confirmed by gene immunization.