Objective To investigate the effects of electroacupuncture at meridian point on the mRNA expressions of corticotropin-releasing factor (CRF) and corticosteroid (CORT) in the hippocampus after cerebral ischemia reperfusion in rats.MethodsA total of 150 SD rats were randomly divided into 5 groups by random number table method: normal control group, sham operation group, model group, meridian point electroacupuncture group and non-meridian point electroacupuncture group, with 30 rats in each group. A model of focal cerebral ischemic reperfusion was induced using the modified intraluminal thread method. In the meridian point electroacupuncture group, at 6 h after cerebral ischemia reperfusion, acupuncture “LI11” on both sides, bilateral “ST36”, “GV20”, ”GV16” daily for 7 d. The neurological deficits were evaluated 1 d, 3 d, and 7 d after cerebral ischemia reperfusion, and then all rats were sacrificed and the hippocampi were harvested. The mRNA expressions of CRF and CORT in the hippocampus were determined by quantitative real-time PCR.ResultsThe mRNA expressions of CRF (1.122 ± 0.249, 1.190 ± 0.666, 0.454 ± 0.612 in the model group; 0.021 ± 0.049, 0.021 ± 0.027, 0.035 ± 0.005 in the sham operation group) and CORT (0.917 ± 0.113, 1.024 ± 0.290, 0.709 ± 0.055 in the model group; 0.016 ± 0.013, 0.016 ± 0.006, 0.043 ± 0.006 in the sham operation group) in the hippocampus 1 d, 3 d, 7 d after cerebral ischemic reperfusion were significantly increased in the model group compared with the sham operation group (all P<0.01). The mRNA expressions of CRF (0.424 ± 0.104, 0.339 ± 0.476, 0.095 ± 0.021) and CORT (0.377 ± 0.073, 0.138 ± 0.025, 0.158 ± 0.010) in the hippocampus 1 d, 3 d, 7 d after cerebral ischemic reperfusion were significantly decreased in the meridian point electroacupuncture group compared with the mode1 group (allP<0.01). The neurological deficits scale 1 d, 3 d, 7 d after cerebral ischemic reperfusion were significantly decreased in the meridian point electroacupuncture group compared with the mode1 group (1.83 ± 0.75, 1.50 ± 0.55 and 1.17±0.41 in the meridian point electroacupuncture group; 2.50 ± 0.84, 2.33 ± 0.52 and 1.67 ± 0.52 in the model graoup; allP<0.01). Conclusion Electricacupuncture at meridian point can reduce the mRNA expressions of CRF and CORT in the hippocampus, and improve neurological deficits after cerebral ischemia reperfusion in rats.

Objective: To understand the effects of different expansion ways on collagen and actin of skin and to provide an experiment foundation for the suitable expansion method clinically. Methods: The rabbits were divided into five groups. The expanders were embedded in experimental group. The sirius-red specific staining method was applied to observe the changes of type I collagen and type III collagen and their proportion. Immunochemistry method was used to detect the expression of actin. Results: 1: In group A, B and C, type I collagen arranged turbulently and was thicker and type III collagen decreased relatively at earlier maintaining period. After 4 week, type I collagen arranged regularly and type III collagen was relatively increased. 2: Comparisons of the area about type I, III collagen: At the same maintaining period, there were no significant difference among the group A,B and C. With the extension of maintaining period, the area of type I, III collagen increased gradually, but there was inclined to stabilize at the fourth week in group A, B and at the third week in group C. Comparison of the proportion of collagen I and collagen III: There were significant difference between group A, B and group D, but no significant difference was observed after the fourth week. There were significant difference between group C and control group, but no significant difference was observed after three week. 3. With the extension of maintaining period, the positive expression rates of actin decreased gradually in group A, B, C. At the same maintaining period, significant difference were observed among group A, B, C. The positive expression rates of actin in group C were lower than that of group A at the same maintaining period. Conclusion: The repeated rapid expansion can not only shorten the clinical period but also promote the quality of skin flap. It is a safe and feasible method in clinic.

Objective To evaluate the effects of nurse-led team management on self-management and hyperten-sion control for community-dwelling hypertensive patients. Methods A quasi-experimental study design was adopt-ed,two communities were elected as the intervention group and the control group in Wuhou District,Chengdu,and 102 hypertensive patients were recruited from each group. The intervention group received nurse-led team manage-ment for 2 years,and intervention methods included individual management,phone or home follow-up,collective inter-vention and so on. While the control group received doctor-led hypertension management. The patients' self-man-agement behaviors and hypertension control were evaluated before the intervention and 6,12,24 months after the intervention. Results After 24-month nurse-led team management,scores of self-management behaviors and hyper-tension control rate of patients in the intervention group were significantly higher than those of patients in the con-trol group(P<0.05). Conclusion Nurse-led team management could significantly improve self-management behaviors and the rate of hypertension control for hypertensive patients.

Medical ethics is the standard ofbehavior toadjust the relationship between medical personnel and patients,medical personnel,medical personnel and society.It is a general social morality in medical field in special performance.Teachers should carry through the ethics education to the end,making students smoothly enter society from the school,cultivating good ethics during the process of learning basic knowledge of oral cavity,and developing high-quality oral physicians for our country.

10.3969/j.issn.2095-4344.2013.23.010

Collagen α3 (IV) chains are one of the major constituent components of the basement membrane in the mammalian testis. Studies have shown that biologically active fragments, such as noncollagenase domain (NC1)-peptide, can be released from the C-terminal region of collagen α3 (IV) chains, possibly through the proteolytic action of metalloproteinase 9 (MMP9). NC1-peptide was shown to promote blood-testis barrier (BTB) remodeling and fully developed spermatid (e.g., sperm) release from the seminiferous epithelium because this bioactive peptide was capable of perturbing the organization of both actin- and microtubule (MT)-based cytoskeletons at the Sertoli cell-cell and also Sertoli-spermatid interface, the ultrastructure known as the basal ectoplasmic specialization (ES) and apical ES, respectively. More importantly, recent studies have shown that this NC1-peptide-induced effects on cytoskeletal organization in the testis are mediated through an activation of mammalian target of rapamycin complex 1/ribosomal protein S6/transforming retrovirus Akt1/2 protein (mTORC1/rpS6/Akt1/2) signaling cascade, involving an activation of cell division control protein 42 homolog (Cdc42) GTPase, but not Ras homolog family member A GTPase (RhoA), and the participation of end-binding protein 1 (EB1), a microtubule plus (+) end tracking protein (+TIP), downstream. Herein, we critically evaluate these findings, providing a critical discussion by which the basement membrane modulates spermatogenesis through one of its locally generated regulatory peptides in the testis.

OBJECTIVETo investigate the proliferation and differentiation of cultured corneal stem cells and determine the effect of corneal stem cells cultured on amniotic membranes on the limbal area for treating corneal burns.

METHODSThe proliferation and differentiation of corneal stem cells in vitro had been examined using colony-forming efficiency and immunohistochemistry. The stem cells had been cultured on amniotic membranes and transplanted to the limbal area for treating corneal burns.

RESULTSCorneal stem cells had a high proliferation capacity in primary and first passage, cytokeratin 3 was not expressed in primary culture but partly in first passage. The stem cells could proliferate to form cell layer on an amniotic membrane. When transplanted, stem cells could survive on limbus. After transplantation, ocular inflammation resolved, the cornea re-epithelialized, the stromal opacity reduced, the superficial neovascularity was lessened and the conjunctival fornix re-established.

CONCLUSIONSOcular surface conditions could be improved by allograft of corneal stem cells cultured on amniotic membranes.

Alkalies ; Amnion ; transplantation ; Animals ; Burns, Chemical ; surgery ; Cell Culture Techniques ; methods ; Cell Differentiation ; Cell Division ; Cell Transplantation ; methods ; Cells, Cultured ; Epithelium, Corneal ; chemistry ; cytology ; Eye Burns ; chemically induced ; surgery ; Graft Survival ; Humans ; Keratins ; analysis ; Limbus Corneae ; chemistry ; cytology ; Rabbits ; Stem Cells ; chemistry ; cytology ; Treatment Outcome

Objective To explore the lived experience of children with acute leukemia about acupoint therapy during chemotherapy, so as to provide the theoretical and practice evidence for development of more scientific nursing measures. Methods Totally 14 children with acute leukemia participated in half-structured interviews regarding their experience of acupoint therapy.The NVivo 10.0 software was used to supervise, excavate and seek data.The data were analyzed by the Co1aizzi phenomenological methodology. Results Five themes were extracted including benefit sense,a sense of accomplishment of self-fulfilling, a sense of attachment to medical, sigh with emotion the magic of traditional Chinese medicine and bearable mild discomfort. Conclusions The experience of children with acute leukemiais positive feelings for the most part, nursing staff should listen to them and give corresponding nursing according to specific circumstances in order to improve the quality of nursing.

Objective To survey the status and related demands of advanced clinical training among community general practitioners (GPs) in Shanghai.Methods A survey on the status and demands of advanced clinical training was conducted with a self-designed questionnaire among GPs in Minhang District and Jiading District of Shanghai from January to May 2016.The questionnaire included basic information , experience of advanced clinical training , satisfaction with training and training demands .Results Total 526 questionnaire were distributed and 478 valid questionnaires were returned with a response rate of 90.9%. Among the participants 202 (42.3%) had experience of advanced clinical study ; the training units were mainly secondary hospitals in Shanghai (69.3%, 140/202); 67.2%(131/195) participants considered that the previous training did not fully meet the needs of the community .There was significant difference in the training demands among participants with different ages , professional titles, and working years ( χ2＝12.754, 7.912, 4.501, all P＜0.01); those with younger age, higher education and shorter working years had higher training demands .Survey showed that 57.3%( 274/478 ) of participants demanded further studies in the future; 58.3%( 239/410) of them chose tertiary hospitals in Shanghai as training unit , 79.0%(321/406) chose internal medicine and geriatrics as training specialties , particularly in outpatient clinic (73.2%, 298/407); the length of study should be 3 months (32.8%, 132/402) or 6 months (27.6%, 111/402), and completed in segments (61.5%, 253/411).The purpose of training was mainly to upgrade clinical competence (94.2%, 258/272); busy working schedule (53.4%, 66/116) was the main reason for not intending the advanced clinical studies .Conclusions The community general practitioners in Shanghai have high demands for advanced clinical training .The training should meet the needs of clinical practice in community and the mode of study should be more flexible .

Objective To explore the real experience of parents of children with acute leukemia in children undergoing first chemotherapy so as to provide the theoretical and practical evidence for development of individualized nursing measures.Methods A total of 16 parents with acute leukemia in children undergoing first chemotherapy were investigated using half-structured interviews via phenomenological research methods. The NVivo10.0 software was used to manage, discover and seek data. The data were analyzed using Colaizzi phenomenological method.Results Three themes including complex negative emotions, excessive economic burden and multiply needs were extracted.Conclusions Parents with acute leukemia in children undergoing first chemotherapy need more attentions. Nurses should take individualized measures to really meet their needs and reduce psychological load so as to be better with follow-up treatment of children.