Objective Cerebral ischemia would rapidly initiate structural and functional damages in brain, including blood-brain barrier disruption, inflammation, and angiogenesis. The purpose of present study is to investigate the effects of indomethacin, an agent that inhibits cyclooxygenase, on the inflammatory reaction after cerebral ischemia-reperfusion in transient middle cerebral artery occlusion(MCAO)rats and its possible mechanism. Methods Adult male SD rats weighed 250-250g were subjected to either sham surgery or middle cerebral artery occlusion (MCAO) for 2h of brain ischemia and 24h reperfusion. After 24h of reperfusion,the size of cerebral infarction and the neurological deficit were determined by the method of TTC (2,3,5-Triphenyltetrazolium Chloride) staining and Longa's score analysis.The contents of IL-8, IL-1?, TNF-? and MPO in brain tissue were assayed by ELISA. The expressions of ICAM-1 ande E-selectin were evaluated with Western-blot. Results It was observed that indomethacin (6 or 9mg/kg i.p pretreatment for 5d, once a day) significantly reduced cerebral infarct volume and ameliorated the neurological deficit (P

Objective To detect mite allergens in the dust of air conditioner filter-net and floating air in room. Methods Samples were collected from rooms of asthma patient and normal families with or without air conditioner. Der p 1,Der f 1 and Der 2 were determined by two monoclonal antibody-based ELISA. Results In asthma patient families,the concentration of airborne Der p 1,Der f 1 and Der 2 was (0.23 ? 0.13),(2.62 ? 1.08),(0.93 ? 0.41) ng/m3,and (0.56 ? 0.25),(4.74 ? 1.22),(2.33 ? 0.64) ng/m3 respectively before and after the air conditioner switched on,all showing a significant difference (P

AIM: To study the interaction effects of Astragalus polysaccharide(APS) and total glucosides of paeony(TGP) with various dosage on the platelet aggregation. METHODS: Platelet aggregation was estimated by the turbidimetric method. The best interaction effects were determined by uniform design method. RESULTS: APS showed apparent anti-platelet effects and better combination with TGP,and the ratio of 121 was the best one. CONCLUSION: APS concerted with TGP inhibits platelet aggregation.

OBJECTIVE: To observe the effects of Pollen Typhae total flavone (PTF) on glucose and lipid metabolism in 3T3-L1 adipocytes. METHODS: The content of glucose which disappeared from the culture medium after incubation with drugs for 24 hours was determined as glucose consumption of the cells. The activity of cells was detected by XTT method. The transport of glucose was observed by (3)H-glucose uptake method. The efflux of free fatty acid (FFA) from adipocytes was observed by the concentration of FFA in the culture medium. RESULTS: The glucose concentration in culture medium was significantly decreased with a concentration-dependent effect, when PTF concentrations were from 0.025 g/L to 0.4 g/L. The toxic effect on cells appeared while PTF concentration was 0.4 g/L, and the MTT value decreased. PTF also significantly increased glucose transportation in the 3T3-L1 adipocytes as rosiglitazone (ROS) did. At the same time, FFA concentration in culture medium was significantly decreased as compared to the normal control group, while ROS-treated group did not show any difference. CONCLUSION: PTF can increase insulin sensitivity by increasing glucose transportation and consumption in the 3T3-L1 adipocytes as well as decreasing the FFA efflux from the cells.

Objective: To analyze the prevalence of silicosis in the militias with contribution to the former Xiangyu Railway construction in Nanchong, China, from 2008 to 2017, and to provide a scientific basis for the future development of pneumoconiosis prevention and control strategies based on the current status. Methods: A database was established for the data of 11863 militias with contribution to the former Xiangyu Railway construction in Nanchong, China, who attended our hospital from 2008 to 2017, including 11485 males (96.81%) and 378 females (3.19%). The SPSS 17.0 software was used to analyze the differences in the detection rate of silicosis between militias with different sexes or those with different job types, as well as the age, length of service, and regional distribution of patients. Results: Of the 11863 railway militias in Nanchong, 3169 (26.71%) were diagnosed with silicosis, including 2761 (87.12%) in stage I, 359 (11.33%) in stage II, and 49 (1.55%) in stage III; the males had a significantly higher detection rate of silicosis than the females (χ²=64.496, P<0.05); there was a significant difference in the detection rate of silicosis between the militias with different job types (χ²=856.839, P<0.05). There were significant differences in the mean age of diagnosis and the mean length of service between the patients with different stages of silicosis (F=4.944, P<0.05; F=3.174, P<0.05). There was a significant difference in the number of militia patients with silicosis between different areas of Nanchong (χ²=843.330, P<0.05). Conclusion The prevalence of silicosis is relatively high among the militias with contribution to the former Xiangyu Railway. It is necessary to strengthen the occupational health monitoring of patients, actively treat and prevent complications, and improve the quality of life of patients.

Objective Analyzing the performance of microbial barrier of needleless positive pressure closed connectors. Methods Three kinds of brand needleless positive pressure closed connectors were chosen to do the access of microorganisms test. Results Positive results were detected in the three experimental groups. Conclusions So far, lots of the connectors are not qualified strictly on the market, some improvement is required in the structure design and process.

Objective The aim of this paper is to study the hemostasis and security of soluble hemostatic gauze in the rabbit liver hemorrhage model.Methods After making the rabbit liver hemorrhage model, the control group used sterile gauze to stop bleeding, the positive control group used TISTAT to stop bleeding, the test group used soluble hemostatic gauze to stop bleeding. Hemostasis, blood loss and animal clinical symptoms were measured. Liver and kidney parameters, along with histopathology were recorded and analyzed. Transmission electron microscopy examination was also done. Results The blood loss is cut back and hemostasis is shortened in the test group. Other tests have no difference with the control group.Conclusion No toxic effects on rabbit are found in the test group. The hemostatic effects have no difference with positive control group.

OBJECTIVETo evaluate the effect of aorta-iliac bypass total thoracoabdominal aorta aneurysm repair to spinal cord function.

METHODSThis was a prospective study. From June 2014 to April 2015, 31 patients underwent total thoracoabdominal aorta aneurysm repair were treated with aorta-iliac bypass technique. There were 23 male and 8 female patients with a mean age of (36±12) years. A 4-branched tetrafurcate graft was used. The aorta-iliac bypass was established, then distal descending aorta was perfused in a retrograde fashion via bypass graft. Thoracic and abdominal aorta were replaced in a staged fashion. Evoked potentials (EP) monitoring was adopted to assess the spinal cord ischemia throughout the procedure. The intraoperative evoked potentials results, clinical outcomes and follow-up results of this technique were evaluated.

RESULTSThe EP wave disappeared after proximal descending aorta clamped and gradually recovered after the patent segmental arteries reattached. Motor evoked potentials disappeared for (56±18) minutes, somatosensory evoked potentials disappeared for (50±19) minutes. The EP wave was restored to normal at the end of operation in all cases. The somatosensory evoked potentials remained unchanged in 2 cases (false negative). One case died after operation. There were acute kidney dysfunction in 3 cases, and pulmonary haemorrhage in 1 case. No spinal cord injure occurred. The median follow-up after operation was 8 months (ranging from 1 to 11 months). There was no delayed neurologic deficit or relative death.

CONCLUSIONSThere is a transient function loss of spinal cord during the aorta-iliac bypass total thoracoabdominal aorta aneurysm repair. But the process is reversible. The technique of the aorta-iliac bypass is practicable.

Adult ; Aorta, Abdominal ; surgery ; Aortic Aneurysm, Abdominal ; surgery ; Aortic Aneurysm, Thoracic ; surgery ; Evoked Potentials, Motor ; Evoked Potentials, Somatosensory ; Female ; Humans ; Male ; Middle Aged ; Prospective Studies ; Spinal Cord ; physiopathology ; Vascular Surgical Procedures ; adverse effects

This paper reports a case of fetomaternal hemorrhage (FMH), which was diagnosed as choriocarcinoma due to late postpartum hemorrhage 20 days after full-term vaginal delivery. The patient fully recovered after chemotherapy combined with surgical treatment, and no disease progression was observed during a one-year follow-up. Choriocarcinoma is one of the risk factors for FMH. Therefore, it is suggested that the placenta should be examined in any suspected cases of FMH. Maternal human chorionic gonadotrophin (hCG) level should also be monitored after delivery for early diagnosis and better prognosis of choriocarcinoma.

Objective:To explore the clinical characteristics of hemoglobin H (HbH) disease during pregnancy, and evaluate the impact of prepregnant anemia on maternal and fetal outcomes.Methods:Clinical data of 12 pregnant women, who were diagnosed with HbH disease by genetic testing in the Third Affiliated Hospital of Guangzhou Medical University from January 1, 2017, to December 31, 2022, were retrospectively collected. Thalassemia genotypes, general conditions before and during pregnancy, and perinatal outcomes were analyzed using descriptive analysis.Results:The 12 patients were categorized into moderate anemia (Hb<90 g/L, n=5) and mild anemia (Hb≥90 g/L and <110 g/L, n=7) groups according to the level of Hb before conception. In the moderate anemia group, with one deletional HbH disease and four non-deletional type, all had blood transfusion histories, and four developed severe anemia during pregnancy with four cases of fetal growth restriction. The mild anemia group comprised six deletional and one non-deletional genotype, with no severe anemia or transfusion requirement during pregnancy. In terms of maternal and fetal outcomes, two moderate anemia cases opted for pregnancy termination, while three delivered via cesarean section with two preterm infants having mild asphyxia and one full-term. In the mild anemia group, one case terminated due to intrauterine fetal death, and the remaining six continued the pregnancies, including two were delivered normally, one was assisted by forceps, and three through cesarean section. Among the six delivered cases, one was preterm birth, one infant was born with severe asphyxia and four mothers developed postpartum complications such as puerperal infection and postpartum hemorrhage. Conclusions:Clinical manifestations of HbH disease vary greatly. Individualized management should be offered based on the degree of anemia before pregnancy. For women with moderate anemia, close monitoring and appropriate intervention are required during pregnancy to improve both maternal and infant outcomes.