Osteoporosis is a bone pathology leading to increased fracture risk and challenging the quality of life. The aim of this study was to evaluate the effect of an anthraquinone glycoside, aloin, on osteogenic induction of MC3T3-E1 cells. Aloin increased alkaline phosphatase (ALP) activity, an early differentiation marker of osteoblasts. Aloin also increased the ALP activity in adult human adipose-derived stem cells (hADSC), indicating that the action of aloin was not cell-type specific. Alizarin red S staining revealed a significant amount of calcium deposition in cells treated with aloin. Aloin enhanced the expression of osteoblast differentiation genes, Bmp-2, Runx2 and collagen 1a, in a dose-dependent manner. Western blot analysis revealed that noggin and inhibitors of p38 MAPK and SAPK/JNK signals attenuated aloin-promoted expressions of Bmp-2 and Runx2 proteins. siRNA mediated blocking of Wnt-5a signaling pathway also annulled the influence of aloin, indicating Wnt-5a dependent activity. Inhibition of the different signal pathways abrogated the influence of aloin on ALP activity, confirming that aloin induced MC3T3-E1 cells into osteoblasts through MAPK mediated Wnt and Bmp signaling pathway.
Adult ; Alkaline Phosphatase ; Blotting, Western ; Calcium ; Collagen ; Core Binding Factor Alpha 1 Subunit ; Humans ; Osteoblasts ; Osteoporosis ; p38 Mitogen-Activated Protein Kinases ; Pathology ; Quality of Life ; RNA, Small Interfering ; Signal Transduction ; Stem Cells

Hemodialysis patients need to acquire exercise habits because physical inactivity in hemodialysis patients is associated with increased mortality. However, factors associated with exercise habits in hemodialysis patients are unknown. The purpose of this study was to investigate the factors related to exercise habits in hemodialysis patients. We measured 104 hemodialysis patients' exercise habits (transtheoretical model：TTM), laboratory data, self-efficacy (SE), short physical performance battery and grip strength, nutritional index, dialysis vintage, fluid removal, and Kt/V. Multiple linear regression analysis was carried out to assess the association of the aforementioned factors with TTM. As a result, in multiple linear regression analysis, SE, age, and Kt/V were associated with TTM (R2＝0.34, p＜0.05). hemodialysis patients may require mental support, such as SE and adequate dialysis dose, to acquire exercise habits.

Hemodialysis patients need to acquire exercise habits because physical inactivity in hemodialysis patients is associated with increased mortality. However, factors associated with exercise habits in hemodialysis patients are unknown. The purpose of this study was to investigate the factors related to exercise habits in hemodialysis patients. We measured 104 hemodialysis patients' exercise habits (transtheoretical model：TTM), laboratory data, self-efficacy (SE), short physical performance battery and grip strength, nutritional index, dialysis vintage, fluid removal, and Kt/V. Multiple linear regression analysis was carried out to assess the association of the aforementioned factors with TTM. As a result, in multiple linear regression analysis, SE, age, and Kt/V were associated with TTM (R2＝0.34, p＜0.05). hemodialysis patients may require mental support, such as SE and adequate dialysis dose, to acquire exercise habits.